Polypeptide drug for treating diseases caused by highly pathogenic avian influenza virus H5N1

An avian influenza virus, H5N1 technology, applied in the field of biomedicine, can solve the problems of reduced replication efficiency in mice, and achieve the effect of easy automated mass production, easy transportation and storage, and obvious effects

Inactive Publication Date: 2011-05-18
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Watanabe et al. found that after cutting off 11 amino acids at the hydroxyl end of H5N1 virus M2, the virus had similar growth ability compared with the wild type, but the replication efficiency in mice was greatly reduced

Method used

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  • Polypeptide drug for treating diseases caused by highly pathogenic avian influenza virus H5N1
  • Polypeptide drug for treating diseases caused by highly pathogenic avian influenza virus H5N1
  • Polypeptide drug for treating diseases caused by highly pathogenic avian influenza virus H5N1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: its amino acid sequence can be:

[0024]

[0025] The polypeptide can stimulate HLA-A*0201 positive human peripheral blood mononuclear cells in vitro, increase the number of these peripheral blood mononuclear cells transformed into cytotoxic T lymphocytes (CTL for short) by 60%, and activate CTL to dissolve For cells with the H5 gene of the highly pathogenic avian influenza virus H5N1, the lysis rate is 83.10%.

Embodiment 2

[0026] Embodiment 2: its amino acid sequence can also be:

[0027]

[0028] The polypeptide can stimulate peripheral blood mononuclear cells of HLA-A*0201 positive human body in vitro, increase the number of these peripheral blood mononuclear cells transformed into cytotoxic T lymphocytes (CTL for short) by 50%, and activate CTL to dissolve For cells with the H5 gene of the highly pathogenic avian influenza virus H5N1, the lysis rate is 79.0%.

Embodiment 3

[0029] Embodiment 3: its amino acid sequence can also be:

[0030]

[0031] The polypeptide can stimulate HLA-A*0201 positive human peripheral blood mononuclear cells in vitro, increase the number of these peripheral blood mononuclear cells transformed into cytotoxic T lymphocytes (CTL for short) by 55%, and activate CTL to dissolve For cells with the H5 gene of the highly pathogenic avian influenza virus H5N1, the lysis rate is 68.0%.

[0032] The amino acid sequence method described above is an existing mature technology, which is made according to the following method:

[0033] Adopt the standard Fmoc scheme, start to select 0.0125mmol, PSC resin (produced by ABI company, lot number A5F013), respectively according to the sequence feature described in the claim, make the peptide chain extend one by one from the C end to the N end, each amino acid raw material (U.S. ABI Company production) consumption is 0.1mmol. Various amino acid protection groups are: the alpha amino ...

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PUM

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Abstract

The invention relates to a polypeptide drug for treating diseases caused by highly pathogenic avian influenza virus H5N1, in particular to a polypeptide drug about diseases caused by highly pathogenic avian influenza virus H5N1, belonging to the field of biomedicine. The polypeptide drug is characterized in that the amino acid sequence of the polypeptide drug is amido-FLNVEWSYI-carboxyl, which is a special connection mode formed by using the characteristic that lysine has two amidoes and one carboxyl and using the lysine as a joint. The invention has the advantages of convenience for transportation and preservation and automated batch production; the polypeptide drug has the capacity of activating specific cytotoxicity T lymphocyte (CTL); and the specific CTL cells have target cells for killing and dissolving H5 protein having the highly pathogenic avian influenza virus H5N1, with obvious effects but without toxic or side effects.

Description

(1) Technical field [0001] The invention relates to the field of biomedicine, in particular to a polypeptide drug for diseases caused by highly pathogenic avian influenza virus H5N1. (two), background technology [0002] Avian influenza (Avian influenza) is abbreviated as bird flu, which is a widespread infectious disease in poultry caused by type A influenza virus (AIV). According to incomplete statistics, the global bird flu epidemic has spread to 47 countries and regions, and there have been more than 195 cases of human bird flu, of which at least 110 people died, with a mortality rate as high as 56.4%. The avian flu that occurred in Hong Kong in 1997 caused human morbidity and death, which aroused great attention from all over the world. AIV can be divided into 16 H subtypes (H1-H16) and 10 N subtypes (N1-N10) according to the differences in the antigenicity of hemagglutinin (HA) and neuraminidase (NA). [Alexander DJ. A review of avian influenza in different birdspecie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61K38/10A61K38/16C07K7/06C07K7/08C07K14/00A61P31/16
Inventor 吕凤林田菲菲杨力
Owner CHONGQING UNIV
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