Method of biochip for simultaneous testing avian influenza infection of human and fowls

An avian influenza virus and biochip technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as time-consuming and labor-intensive, and achieve the effect of protecting people's lives, property and animal resources.

Inactive Publication Date: 2007-07-18
深圳市赛尔生物技术有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Therefore, it is time-consuming and labor-intensive to identify so many types by traditional methods
At present, there

Method used

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  • Method of biochip for simultaneous testing avian influenza infection of human and fowls

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Embodiment 1

[0024] A: Prepare the activated 3D chip substrate (produced by Shenzhen Saier Biotechnology Co., Ltd.);

[0025] B: Fragments of various subtypes of avian influenza to be spotted: namely common hemagglutinin fragments H1, H3, H5, H7, H9, neuraminidase fragments N1, N2, nucleoprotein NP, non-structural protein NS1. Specimens are prepared with monoclonal antibodies of H1, H3, H5, H7, H9 and N1, N2, NP, and NS1, that is, the ascites is purified and diluted to different concentrations, and then mixed in proportion and all mixed, as the specimen to be tested. Meanwhile, normal human serum and chicken serum were used as controls, respectively. In practical application, the specimen can be human serum, chicken serum. and require it to be carried out in a specific laboratory. These fragment samples are all antigen fragments expressed by genetic engineering (co-developed by Shenzhen Saier Biotechnology Co., Ltd. and a research institute), all of which are expressed in yeast, with goo...

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Abstract

A biochip for detecting avain fluenza virus of both human and fowls is prepared as providing activated chip substrate, providing various fluenza virus subset sections to be sample-set, providing quality control protein, carrying out arrangement pattern design of point measurement, making sample-sets of quality control protein and various avain fluenza virus subset sections according to prepared pattern for detecting out antibodies generated by various subsets simultaneously.

Description

technical field [0001] The invention relates to a method for detecting human and avian avian influenza virus (AIV) infection in the field of virus detection, in particular to a method for detecting and screening high pathogenicity, low pathogenicity and non-pathogenicity by a protein chip method Development of a method for the detection of avian influenza virus (AIV). Background technique [0002] Avian influenza is an infectious disease of birds caused by influenza A virus. Avian influenza virus infection can manifest as mild respiratory symptoms and gastrointestinal symptoms, with a low mortality rate; or with more severe systemic, hemorrhagic, and septic symptoms, with a high mortality rate. This difference in symptoms is mainly determined by the virulence of the avian influenza virus. [0003] According to the different pathogenicity and virulence of avian influenza virus, avian influenza can be divided into highly pathogenic avian influenza, low pathogenic avian influ...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/569
Inventor 林连成
Owner 深圳市赛尔生物技术有限公司
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