34results about How to "Highly pathogenic" patented technology

The invention discloses a negative pressure ambulance used to prevent nosocomial infection, containing car body, hermetic board cabin connected with car body chassis, control device; the hermetic board cabin is consisted of nose functional cabin and medical afterhold; the nose functional cabin is the flow direction of air controlling cell, the medical afterhold contains cabin air inlet chamber, cabin exhaust chamber, also a medical equipment in cabin; the medical afterhold sets closable port; the cabin air inlet chamber of medical afterhold and air mixing chamber of nose functional cabin can be connected, the cabin exhaust chamber of medical afterhold and the air filtration disinfection chamber can be connected. The air flow of hermetic board cabin has three kinds of state, the air in cabin can all realize directional airflow and pressure gradient. This car can be used as normal ambulance, high pathogenic infectious patient ambulance, various diseases checking car, mobile p3 lab, carrier vehicle of biological bacteria danger foe things; it can be asepsis operation car after switching, high infectivity, strong virulence weapon battlefield command, arm carrying car.

The invention discloses a sclerotium rolfsii solid fermentation culture medium and an application thereof. The solid fermentation culture medium comprises a solid substrate and water, wherein the solid substrate comprises wheat bran and agricultural wastes, the wheat bran accounts for 20%-60% of the total weight of the solid substrate, and the weight ratio of the water to the solid substrate is (20-45):100; and the agricultural wastes select rice husk, sweet sorghum straw, an organic fertilizer, a wheat bran substrate, vinegar residue, wine lees, garden dead twigs or sawdust. The sclerotium rolfsii solid fermentation culture medium prepared by the preparation method disclosed by the invention is low in cost, the preparation process is simple, and a herbicide prepared by adopting the solid fermentation culture medium can be preserved at room temperature.

A process for preparing the efficient bacterial herbicide includes sampling the soil near the root of weeds such as crabgrass herb, caper euphorbia, cassia, etc. or their stem or leaves, separating with NPC culture medium, and the chlorella pyrenoidosa and glutamine synthetase depressants, and screening the bacterial strains with high herbiciding activity and broad spectrum. Its advantage is highherbiciding effect.

The invention discloses a multi-gene detection method of Listeria monocytogenes based on a quantum dot/graphene oxide nanometer platform and belongs to the technical field of gene nanometer detection. The multi-gene detection method of Listeria monocytogenes comprises the following steps of: selecting two or more than two genes of the Listeria monocytogenes as target points of genetic detection and designing two pairs or more than two pairs of genetic PCR (Polymerase Chain Reaction) amplimers according to the analysis result of a gene conserved area; amplifying two or more than two genetic special sequences at the same time according to the principle of LATE-PCR to obtain a corresponding single stranded amplification product; then, hybridizing the single stranded amplification product with a quantum dot fluorescence probe; and finally, quenching and removing the non-hybridized quantum dot probe by using graphene oxide. When the target gene does not exist, all quantum dot fluorescence probes are quenched. On the contrary, when the target gene exists, corresponding fluorescent signals are obtained. The detection method has high detection reliability to Listeria monocytogenes.

The invention discloses a biological weeding organic fertilizer. The biological weeding organic fertilizer is a solid granule prepared through wrapping a base granule with Sclerotium rolfsii Sacc. SC64 mycelia; a mass ratio of the Sclerotium rolfsii Sacc. SC64 mycelia to the base granule s 1.7-2.0:1; and the base granule is prepared from, by mass, 5-45% of an organic fertilizer, 1.3-28.6% of an adhesive, 10-15% of water, and the balance of a solid matrix. The invention also discloses a preparation method of the biological weeding organic fertilizer, and an application of the biological weeding organic fertilizer in control of dicotyledon weeds. The biological weeding organic fertilizer simultaneously has the advantages of a traditional organic fertilizer and a biological herbicide, so garden wastes and crop straws are fully used to realize changing of wastes into valuables and reduce environment pollution caused by straw burning, and the use of chemical fertilizers and pesticides is reduced to enhance the stress resistance of crops and improve the quality of the crops.

The invention relates to the field of virology and aims to provide an avian infectious bronchitis virus natural mutant, kept in China General Microbiological Culture Collection Center, named infectious bronchitis virus and collected under CGMCC No. 11491. A genome of this mutant is 27, 541 lbp in length, a complete genome sequence of the mutant is as shown in SEQ ID NO: 1, with structural characteristics of a typical IBV genome coding structure. This mutant having special genes inserted in and provided by the invention can break the immunity protection of vaccines to breed in an organism, this mutant can proliferate in susceptible chickens without causing significant pathogenicity to sensitive chickens, and thus this mutant can serve as base material for the further study on mutation mechanism of an attenuated vaccine or IBV virus strain.

The invention discloses a Nimbya alternantherae effector Na2-g9900, and a protein and an application thereof. The nucleotide full-length sequence of the effector gene is represented by SEQ ID NO.1, the open reading frame sequence is represented by SEQ ID NO.2, and the amino acid sequence of the effector Na2-g9900 protein is represented by SEQ ID NO.3. The effector Na2-g9900 found IN the inventionhas significant pathogenicity to Alternanthera Philoxeroides, can be used for preparing preparation products for controlling Alternanthera Philoxeroides, can also be used for constructing biocontrol bacteria, and has an excellent application prospect in the treatment of Alternanthera philoxeroides.

The invention provides a bio-organic fertilizer as well as a production method and an application thereof and relates to the technical field of bio-organic fertilizers. Crushed and sterilized crop straw and an organic potassium fertilizer are mixed and then mixed with glutinous rice flour to be made into granules, the granules are sterilized, and sterile granules are obtained; the sterile granules are mixed with Sclerotium rolfsii Sacc, so that Sclerotium rolfsii Sacc wraps the sterile granules, and the bio-organic fertilizer is obtained after drying. The Sclerotium rolfsii Sacc wraps the sterile granules, can be quickly attached to the roots of weeds and crop straw after the bio-organic fertilizer is scattered in direct-sowing paddy water for crop rotation of rice and wheat, has a quicker infection function and can quickly perform the functions of weeding and straw composting. The Sclerotium-rolfsii-Sacc-containing bio-organic fertilizer prepared with the method is low in cost, simple in preparation process and can be stored at normal temperature.

A novel transgenic sugar beet event designated GM RZ13 is disclosed. The invention relates to nucleic acids that are unique to event GM RZ13. The invention also relates to assays for detecting the presence of the GM RZ13 event based on DNA sequences of the recombinant constructs inserted into the sugar beet genome that resulted in the GM RZ13 event and of genomic sequences flanking the insertion site. The invention further relates to sugar beet plants comprising the genotype of GM RZ13 and to methods for producing a sugar beet plant by crossing a sugar beet plant comprising the GM RZ13 genotype with itself or another sugar beet variety. Seeds of sugar beet plants comprising the GM RZ13 genotype are also objects of the present invention.

The invention discloses a reference product for detecting pathogenic microorganisms of bloodstream infection and a preparation method thereof. The preparation method comprises the following steps: (1) respectively obtaining genomic DNAs (Deoxyribose Nucleic Acid) of human cells and pathogenic microorganisms; (2) fragmenting the genomic DNAs of human cells and pathogenic microorganisms by using an enzyme digestion method; (3) recovering and purifying the fragmented DNA; (4) valuing and mixing the fragmented DNA; (5) mixing the fragmented DNA and the plasma; and (6) extraction and quality inspection of the reference product for detecting bloodstream infection. The reference product for detecting the pathogenic microorganisms of bloodstream infection is prepared by using the preparation method. The reference product has a stable source, is close to a natural plasma sample, covers various pathogens, is more accurate in valuing, can be used for performing quality evaluation on a detection result of bloodstream infection detection based on high-throughput sequencing, can be used for comparing results of different sequencing platforms, detection processes and sampling modes, and formulating a technical standard for bloodstream infection detection based on the high-throughput sequencing.

The invention provides a construction method of infectivity clones for black nightshade curtoviruses. The method comprises the steps of respectively designing primers P1 and P2, and P3 and P4, using pLB-NCTV as formwork, and performing PCR amplification of a first copy segment of NCTV and a second cope segment of NCTV; designing primers P5 and P6, using a pCB301 carrier as a formwork, performing PCR, and using the product as a carrier framework of NCTV infectivity clones; and constructing the first copy fragment and the second copy fragment by a multi-segment homologous recombination techniqueto the carrier framework to obtain the infectivity clones containing two copy fragments of NCTV. A construction system of infectivity clones for black nightshade curtoviruses is established, so thatthe high fidelity of a virus sequence is guaranteed, the pathogenicity is high, the construction success rate of the virus infectivity clones is high, and the infection efficiency is high. Besides, the invention further provides a black nightshade curtoviruses freeze-dry positive sample, and the method has important significance in further research on the black nightshade curtoviruses.