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A kind of influenza A virus subtyping detection method

A type A influenza virus and detection method technology, applied in the field of biotechnology detection, can solve problems such as lack of good detection methods, and achieve the effect of improving efficiency and specificity detection

Active Publication Date: 2022-02-11
宁波怡和医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are currently no good detection methods for these subtypes

Method used

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  • A kind of influenza A virus subtyping detection method
  • A kind of influenza A virus subtyping detection method
  • A kind of influenza A virus subtyping detection method

Examples

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Embodiment 1

[0029] 1. Design and synthesis of primers

[0030] According to the HA gene sequences of influenza A viruses H1N1, H5N1 and H7N9 in the Genebank database, design corresponding core primers, reverse primers with fluorescent labels at the 5' end (abbreviated as reverse primers in Table 1) and forward primers, and the sequences are as shown in the table 1.

[0031] Table 1 Primer Sequence

[0032]

[0033] Connect the fluorescent label CY5 to the 5' end of the H1N1 reverse primer to form a fluorescently labeled CY5 H1N1 reverse primer; connect the fluorescent label VIC to the 5' end of the H5N1 reverse primer to form a fluorescently labeled VIC H5N1 reverse primer; A fluorescently labeled FAM was connected to the 5' end of the H7N9 reverse primer to form a fluorescently labeled FAM-labeled H7N9 reverse primer.

[0034] In this example, the linking molecule at the 5' end is -NH 2 -(CH 2 ) 6 -. Therefore, the corresponding forward primers for H1N1, H5N1 and H7N9 are 5’-NH ...

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PUM

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Abstract

The invention discloses a subtype detection method of influenza A virus, which comprises the following steps: Step 1, designing and synthesizing primers; Step 2, preparing carboxyl magnetic beads connected with magnetic beads and forward primers; Step 3, A Influenza virus RNA extraction; Step 4, RT‑PCR; Step 5, magnetic adsorption and washing; Step 6, detection. The influenza A virus subtype detection method of the present invention can perform high-throughput, rapid, sensitive and specific typing of the influenza A virus subtype, and has good application value.

Description

technical field [0001] The invention belongs to the field of biotechnology detection, in particular to a subtype detection method of influenza A virus. Background technique [0002] Influenza A virus is a common influenza virus that mutates very easily, so there are many subtypes of influenza A virus. These subtypes are known as "bird flu". Influenza A is highly pathogenic to humans and has caused worldwide pandemics many times. Among the influenza A viruses, the subtypes of avian influenza viruses that can directly infect humans have been found so far: A H1N1, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 and H10N8. Among them, H1, H5, and H7 subtypes are highly pathogenic, and H1N1, H5N1, and H7N9 deserve special attention. Therefore, it is very necessary to detect influenza A virus subtypes H1N1, H5N1, and H7N9. There are currently no good detection methods for these subtypes. Contents of the invention [0003] The purpose of the present invention is to provide a method...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686
CPCC12Q1/686C12Q1/701C12Q2563/143C12Q2563/149C12Q2563/107C12Q2523/308
Inventor 陈小芬
Owner 宁波怡和医药科技有限公司
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