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Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

An avian influenza virus and influenza virus technology, which is applied in the cultivation devices of tissue cells/viruses, biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of economic and foreign trade losses, and achieve the effect of low price.

Inactive Publication Date: 2005-06-01
SUN YAT SEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, even if the laboratory proves that the isolate is of low pathogenicity, the chickens are treated as strictly as highly pathogenic influenza, thus causing great losses to the economy and foreign trade

Method used

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  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection
  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection
  • Method and device for identifying pathogenic influence of concurrent bacteria infection against poultry influenza virus infection

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Embodiment Construction

[0027] refer to figure 1 and figure 2 , the used cell in vitro homogeneous culture device of the present invention method is made of cell culture bottle 1 and bacteria culture room 3, and bacteria culture room is placed in the cell culture bottle; This cell culture bottle can adopt conventional Kjeldahl cell culture bottle; The bacteria culture The chamber consists of a sealing ring 33 sandwiched between a wall 31 and b wall 32, and a number of fixing screws 35 are fixed to form a closed structure, so that bacteria cannot contact the cells in the cell culture bottle; there are openings (holes) on the b wall 36. The opening is closed with a microporous filter membrane 34 with a pore size not greater than 0.22 microns as an isolation barrier, so that the culture solution in the cell culture bottle can enter the bacterial culture chamber, and at the same time, the enzyme secreted by the bacteria can enter the cell culture through the microporous filter membrane The culture medi...

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Abstract

The invention relates to a method for identifying the influence of concurrent bacterial infection on the pathogenicity of avian influenza virus infection and a device used therefor. Co-inoculate bacteria that secrete subtilisin-like protease or / and bacteria that secrete tryptase and low-pathogenic avian influenza virus in the CEF culture, so that the bacteria cannot directly contact CPE but the enzymes they secrete can enter the culture medium, continue Cultivate and observe the presence or absence of CPE; according to the appearance of CPE, the cause of a large number of chicken deaths can be identified. The cell in vitro homogeneous culture device used in the method is composed of a cell culture bottle and a bacteria culture room, and the bacteria culture room is a closed structure with a microporous filter membrane with a pore size not greater than 0.22 microns that can communicate with the cell culture bottle. The method of the invention is simple and easy to operate, the identification time is short, the result is accurate and reliable, and the device used is low in price, and has important significance for the detection and prevention of bird flu.

Description

technical field [0001] The invention relates to a method for identifying the influence of concurrent bacterial infection on the pathogenicity of avian influenza virus infection and an in vitro homogeneous cell culture device used therein. Background technique [0002] Existing bacterial culture is carried out separately from cell culture. If bacterial contamination occurs in the cell flask during the cell culture process, the cell culture and subculture will not be able to go on for a long time. The main reasons are: [0003] a) Cannibalization of cells by bacteria. [0004] b) The effects of toxins secreted by bacteria during growth on the cell membrane or different parts of the cell. [0005] c) The enzymes secreted by bacteria during the growth process affect a certain link of cell growth. [0006] d) During the process of high-speed division, bacteria occupy most of the growth space and absorb a large amount of nutrients, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12Q1/00
Inventor 杨德威刘福安
Owner SUN YAT SEN UNIV
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