Method of biochip for simultaneous testing avian influenza infection of human and fowls

A bird flu virus, biochip technology, applied in the direction of biological testing, measuring devices, material inspection products, etc.

Inactive Publication Date: 2011-01-19
深圳市赛尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is time-consuming and labor-intensive to identify so many types by traditional methods
At present, there is no chip array method to simultaneously detect the prevalence of various subtypes of avian influenza in the world.

Method used

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  • Method of biochip for simultaneous testing avian influenza infection of human and fowls
  • Method of biochip for simultaneous testing avian influenza infection of human and fowls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A: Prepare the activated three-dimensional chip base (produced by Shenzhen Saier Biotechnology Co., Ltd.);

[0025] B: Fragments of each subtype of avian influenza to be sampled: common hemagglutinin fragments H1, H3, H5, H7, H9, neuraminidase fragments N1, N2, nucleoprotein NP, nonstructural protein NS1. Specimens with H1, H3, H5, H7, H9 and N1, N2, NP, NS1 monoclonal antibodies, that is, ascites after purification, diluted to different concentrations, mixed in proportion and all mixed, as the specimen to be tested. At the same time, normal human serum and chicken serum were used as controls, respectively. In practical application, the specimen can be human serum or chicken serum. And require it to be carried out in a specific laboratory. These fragment samples are all antigen fragments expressed by genetic engineering (developed by Shenzhen Saier Biotechnology Co., Ltd. in cooperation with a certain research institute), all of which have yeast expression products. T...

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PUM

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Abstract

A biochip for detecting avain fluenza virus of both human and fowls is prepared as providing activated chip substrate, providing various fluenza virus subset sections to be sample-set, providing quality control protein, carrying out arrangement pattern design of point measurement, making sample-sets of quality control protein and various avain fluenza virus subset sections according to prepared pattern for detecting out antibodies generated by various subsets simultaneously.

Description

technical field [0001] The invention relates to a method for detecting human and poultry avian influenza virus (AIV) infection in the field of virus detection, specifically a protein chip method for detecting and screening high pathogenicity, low pathogenicity and non-pathogenicity Establishment of a method for avian influenza virus (AIV). Background technique [0002] Avian influenza is an infectious disease of poultry caused by type A influenza virus. Avian influenza virus infection can manifest as mild respiratory symptoms and gastrointestinal symptoms with low mortality; or as severe systemic, hemorrhagic and septic symptoms with high mortality. This difference in symptoms is mainly determined by the virulence of the bird flu virus. [0003] According to the pathogenicity and virulence of avian influenza virus, avian influenza can be divided into highly pathogenic avian influenza, low pathogenic avian influenza and apathogenic avian influenza. [0004] Both H and N re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N33/569
Inventor 林连成
Owner 深圳市赛尔生物技术有限公司
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