33 results about "Alternaria helianthi" patented technology

The invention relates to a hybridoma cell strain 4F4 and an antibody thereof. The hybridoma cell strain 4F4 is preserved in China Center for Type Culture Collection on Dec. 27, 2016 with the preservation number of CCTCC NO:C201702. The hybridoma cell strain 4F4 generates an anti-tenuazonic acid monoclonal antibody, specifically detects tenuazonic acid, and is of great significance to detect the tenuazonic acid.

The invention relates to the field of biotechnology, in particular to application of alternaria alternate metabolic products in cucumber rhizoctonia solani prevention and control. In the invention, endophytic fungus of camphor trees is separated from camphor tree living bodies collected from Tianmu mountain in Hangzhou, Zhejiang province by adopting endophytic fungus separation technology, and isnamed alternaria alternata 31 through microbial taxonomy identification, and the strain is stored in China General Microbiological Culture Collector Center (CGMCCC) on December 31, 2010, and the collection register number is CGMCC No.4508. The invention has a most obvious characteristic that the strain liquid can be fermented to produce active metabolic products which have inhibiting effect on pathogenic bacteria of plant fungus diseases such as Rhizoctonia solani, Fusarium oxysporium, Botrytis cinerea and the like, thus alternaria alternate is an important microbial resource in agriculture.

The invention discloses an application of an activator protein prepared from Alternaria tenuissima by microbial fermentation to a pesticide preparation. The activator protein from Alternaria tenuissima which is prepared from Alternaria tenuissima by fermentation and special extraction processes is a single and stable protein with biological activities. The protein can be prepared with glucosamine oligosaccharides and related protein protectants into wettable powder, the protein can be combined with a receptor protein on a plant cell membrane, in order to induce increases of a series of related enzymatic activity and gene expression quantity in the plant, stimulate a series of metabolism regulation in the plant, promote growth of roots, stems and leaves of the plant, increase chlorophyll content, and increase crop yield. The wettable powder prepared by the protein from Alternaria tenuissima completely accords with each national index, and has the effects for preventing and controlling plant virus diseases, greatly improving physiological functions of plants, enhancing disease resistance of plants, and increasing crop output to a certain degree.

The invention relates to a hybridoma cell strain 7G11 and an antibody thereof. The hybridoma cell strain 7G11 is delivered to the China Center for Type Culture Collection for collection on December 27, 2016, with the collection number of CCTCC NO: C201703. The hybridoma cell strain 7G11 can generate a monoclonal antibody capable of resisting streptospora acid, can specifically detect the streptospora acid and has important significance for detection of the streptospora acid.

The invention belongs to the technical field of the biological pesticide and particularly relates to a biocontrol bacterium for controlling plant diseases and a preparation method thereof. The invention has the following main contents: (1) the separation and identification of Bacillussp.CC09 strain; (2) the preparation of Bacillussp.CC09 strain biocontrol microbial inoculum and the applications of the microbial inoculum in the control of plant diseases; and (3) the stability of the Bacillussp.CC09 biocontrol microbial inoculum. The Bacillussp.CC09 biocontrol microbial inoculum is characterized in that the anti-fungal spectrum is wide, the effect is long-lasting, harmful bacteria are difficult to generate drug resistance, the microbial inoculum is safe for human, livestock and crops and is environmentally friendly, etc. The biocontrol bacterium has obvious inhibitory activity for the growth of Fusariumgraminearum, Alternariaalternata, Rhizoctoniasolani and Phytophthoracapsici and especially obvious control effect on wheat powdery mildew and scab.

The invention relates to a method for rapidly detecting alternaria tenuissima ketone acid. The method is based on a horse radish peroxidase catalysis luminol-hydrogen peroxide luminescence system, indirect competitive chemiluminescent enzyme-linked immunosorbent assay of the alternaria tenuissima ketone acid is built, and the method has the advantages of high sensitivity of a chemiluminescence and high specificity of an immunoassay. The detection range of the method is 0.032ng/mL-30.244ng/mL, IC50 (half maximal inhibitory concentration) is 0.973ng/ mL, lowest detection limit is 0.010ng/mL, a linear regression equation is y=49.82-20.14x, and R20=0.9966. Compared with a traditional ELISA (enzyme-linked immunosorbent assay) method, the IC50 and the lowest detection limit are lower and high in specificity, duration time of light-emitting signals is long and can reach several hours, linear detection range is wider, and the method provides bases for research of limit standards of the alternaria tenuissima ketone acid, detection of pollution level of the alternaria tenuissima ketone acid and risk analysis.

The invention provides an aspergillus flavipes strain TR32 having an inhibiting function on phytopathogens and application thereof. The strain TR32 which is obtained through screening is identified and determined that the classification of the strain TR32 is named aspergillus flavipes, the aspergillus flavipes is preserved in CCTCC (China Center for Type Culture Collection), and the preservation number is CCTCC M2018504. Bacteria inhibition experiments on common phytopathogens such as fudarium graminearum, cytospora sp, physalospora piricola and alternaria alternate verify that the strain TR32obtained in the invention has a wide-spectra and efficient bacterial inhibition function on multiple phytopathogens, the strain TR32 can be prepared into a biocontrol agent for preventing and treating the multiple phytopathogens, and a good market application prospect is obtained.

The invention discloses alternaria tenuissima, which is classified and named as alternaria tenuissima of deuteromycotina alternaria with a bacterial strain number of JSNL005-1, and is already preserved in the general microbiology center of China microbe preservation management committee with a preservation number of CGMCC No. 11311 and a preservation date of September 11, 2015. The alternaria tenuissima JSNL 005-1 disclosed by the invention has remarkable effects in aspects of improving growth rate and drought resistance ability of the dendrobium officinale, and the artificial culture can be realized; the reproductive speed is fast; the alternaria tenuissima is convenient for large-scale production, and is good in development and application prospect.

The invention discloses Streptomyces sp.B5017. The Streptomyces sp.B5017 is characterized in that the preservation number is CCTCC NO: M2019648, and the Streptomyces sp.B5017 is classified and named as Streptomyces sp.B5017. Tests show that the marine-derived streptomyces new strain disclosed by the invention has the effect of antagonizing various phytopathogens, and has an inhibiting effect on gloeosporium musarum, colletotrichum gloeosporioides, colletotrichum pearl plum, botryosphaeria dothidea, fusarium oxysporum f.sp.cubense race 4, fusarium oxysporum f.sp.cubense race 1 microspecies or alternaria alternata. Fermentation liquor prepared from the Streptomyces sp.B5017 disclosed by the invention can effectively avoid withered and yellow withering of leaves caused by the fusarium oxysporum f.sp.cubense race 4 and race 1 in potting experiments, which proves that the Streptomyces sp.B5017 is capable of effectively preventing and treating banana wilt and enhancing the disease resistanceof bananas, has good ecological benefits and social benefits, and has a good application prospect when being developed as a biocontrol agent.

The invention discloses an Alterneria tenuissima strain and application thereof in biological weeding. The classified name of the strain is Alterneria tenuissima, the complete name of the strain is Alterneria tenuissima HZ-1, the strain is preserved in the China General Microbiological Culture Collection Center located at the No.3 room, No.1 yard, Beichen west road, Chaoyang district, Beijing city, China on January 11, 2016, and the preservation number is CGMCC No.11992. The Alterneria tenuissima is used for biological weeding of broad-leaved weeds such as galium tricorne, chenopodium album and malva verticillata in a highland barley field, a broad bean field and a pea field and can effectively control and prevent the broad-leaved weeds.

The invention discloses a loquat endophytic unciform trichoderma strain and applications of the loquat endophytic unciform trichoderma strain. The endophytic trichoderma strain is obtained through separation, then morphological and molecular biological identification is carried out, meanwhile, the antibacterial activity of the endophytic trichoderma strain is tested, and thus a material is provided for the development and utilization of the strain; the endophytic trichoderma strain has the antibacterial activity for five phytopathogens, wherein the inhibiting effect for fungi is obviously stronger than that for bacteria, specifically, the inhibiting effect for pyricularia grisea is the strongest and is 83.17%, the antibacterial rate for alternaria tenuissima takes the second place and is 75.10%, the antibacterial rates for fusarium proliferatum and fusarium sambucinum have no obvious differences and are respectively 52.02% and 49.07%, and the antibacterial rate for Xanthomonas oryzae pv.oyzae is the poorest, and is 38.24%. The trichoderma strain has the antibacterial wide spectrum, and has the development and utilization values and prospects.

The invention relates to a microbial herbicide and a preparation method thereof, belonging to the technical field of pesticide production. The microbial herbicide is prepared from the following ingredients in parts by weight: 10-35 parts of spore suspension mother liquor of Alternaria augustiovoidea strains AAEC05-3, 15-25 parts of spore suspension mother liquor of A. amaranthi-3 and 5-12 parts of Tween-80. The preparation method comprises the steps of mixing the two kinds of spore suspension mother liquor according to a certain ratio, then, adding Tween at the spray volume of 100-150mL/m<2>, and carrying out moisture retention for 40-60 hours, thereby obtaining an aqueous microbial herbicide. The herbicide can effectively inhibit Echinochloa crusgalli and Amaranthus retroflexus, is non-toxic and readily degradable, and is a product with development and application prospects.

The invention relates to a yield increasing method of Alternaria tenuissima activator protein. According to the method, spores are prepared, a spore suspension liquid is prepared, and beta-sitosterol and betaine are added to a fermentation culture medium when a strain liquid is cultured, so that the yield of Alternaria tenuissima activator protein is increased. With the adoption of the yield increasing method, the culture medium and the culture conditions are optimized, so that the activator protein is increased by four times or more; ingredients of the culture medium all come from plants, the sources are convenient and safe, the cost is low, and large-scale production is facilitated; dynamic parameters of fermentation in a shaking flask are researched, and a fermentation culture medium suitable for strains to grow is acquired; influences of the inoculation amount, the initial pH value of the fermentation culture medium, the quantity of liquids in the shaking flask, the temperature and the like on fermentation are researched, and the composition of Alternaria tenuissima activator protein culture medium and the optimum fermentation culture conditions in the shaking flask are determined.

The invention discloses a Fermentation method of alternaria tenuissima. The fermentation method comprises the following steps: taking out an alternaria tenuissima culture HZ-1 stored at 4 DEG C; picking up part of bacterial colony and inoculating the same to a fresh PDA culture medium to be cultured, then picking up part of the bacterial colony from the culture medium and inoculating the same to afresh PDA culture medium, and repeating the step 2-3 times to obtain an activated HZ-1 culture which is vigorous in growing power; taking the HZ-1 culture cultured for 7d in a petri dish, perforating8mm bacterial cakes by a perforator, and inoculating the bacterial cakes 10/L into the PDA liquid culture medium; and culturing the cakes in an oscillator, and filtering the mixture to obtain a HZ-1fermenting liquid. According to the fermentation method, the culture HZ-1 is reproduced massively, thereby laying a theoretical foundation for industrial production and further popularization and application of a biological fungicide.

The invention discloses a Nimbya alternantherae effector Na2-g9900, and a protein and an application thereof. The nucleotide full-length sequence of the effector gene is represented by SEQ ID NO.1, the open reading frame sequence is represented by SEQ ID NO.2, and the amino acid sequence of the effector Na2-g9900 protein is represented by SEQ ID NO.3. The effector Na2-g9900 found IN the inventionhas significant pathogenicity to Alternanthera Philoxeroides, can be used for preparing preparation products for controlling Alternanthera Philoxeroides, can also be used for constructing biocontrol bacteria, and has an excellent application prospect in the treatment of Alternanthera philoxeroides.

The invention provides a fungus for converting phlorizin into phloretin and use thereof, the standard strain is classified and named as Alternaria longipes, and is purchased from the Institute of Microbiology, Chinese Academy of Sciences, China. The preservation number is AS3.2875. The invention also provides a method of preparing the phloretin based on the fungus for converting. The fungus provided by the present invention has high yield and no environmental pollution compared with a conventional conversion method.

The invention discloses application of posaconazole to preparation of a bactericide for controlling plant pathogenic bacteria. Determination of indoor drug sensitivity proves that the posaconazole hasgood activity in inhibiting main diseases of important food crops, including wheat scab-causing fusarium graminearum, rice blast-causing magnaporthe oryzae, sheath blight-causing rhizoctonia cerealisand corn leaf spot-causing maize curvularia, has good activity in inhibiting botrytis cinerea, physalospora piricola, valsa ceratosperma and alternaria tenuissima, and has relatively good activity ininhibiting soil-borne diseases such as fusarium oxysporum, which are extremely difficult to control in agricultural production, and the bacteriostatic activity of the posaconazole is even superior tothose of the conventional bactericides carbendazim and tebuconazole. The invention proposes screening of the green small molecular compound posaconazole from the three-dimensional structure of targetprotein for the first time, and the posaconazole is applied to control of important plant diseases in the agricultural production and has a broad market application prospect.

The invention discloses a planting method of dendrobium officinale. According to the invention, by adding inactivated rhizoctonia solani and alternaria tenuissima during rooting, dendrobium officinalecan induce thallus stimulus to generate stress during rooting, and during transplanting, by amplifying the thalli symbiotic with dendrobium officinale, dendrobium officinale can have high survival rate of transplanting and low infection rate, and grow vigorously, thereby increasing the yield of dendrobium officinale.

Construction of trichoderma harzianum engineering strain having strong wide-spectrum pathogenic fungus parasitic capability and applications of the strain are disclosed. That Nox1-mediated H2O2 is an important bacteriostatic component in a process of hyperparasitism of SQR-T037 on pathogenic funguses is found for the first time by the inventor. Research shows that a muton loses capability of hyperparasitism on sclerotinia sclerotiorum, fusarium oxysporum and sclerotium rolfsii when a gene nox1 is knocked out, and when a complementary nox1-expressing muton is constructed, the hyperparasitism capability of a wild type is recovered, thus confirming that the Nox1 directly regulates and controls hyperparasitism of the SQR-T037 on pathogenic funguses. An engineering fungus overexpressing the nox1 is constructed to increase the H2O2 concentration on hypha surfaces and to significantly increase capability of hyperparasitism of botryis cinerea, alternariasp, fusarium oxysporum, sclerotinia sclerotiorum, rhizoctonia solani and sclerotium rolfsii. The strain and the applications can effectively overcome a problem that disease inhibiting effects and field application effects of functional funguses are unstable in application of microbe preparations mainly comprising trichoderma and related fertilizers.

The invention discloses an active ingredient of folium ficus caricae capable of inhibiting and killing plant pathogenic fungus as well as a preparation method and application thereof. The active ingredient is fine extract obtained by further extracting by adopting ethyl acetate or normal hexane as an extraction agent on the basis of extract obtained by adopting ethanol as an extraction solvent. The active ingredient of the folium ficus caricae capable of inhibiting and killing the plant pathogenic fungus can be used for preparing a preparation for inhibiting and killing the plant pathogenic fungus and mushroom pathogenic fungus, and particularly suitable for preparing a preparation for inhibiting and killing rape sclerotinia sclerotiorum, fusarium fungi, alternaria alternate, rhizopus oryzae and other pathogenic fungus. The active ingredient of the folium ficus caricae disclosed in the invention has the characteristics of wide raw material source, nontoxicity to the human body, harmlessness to crops, ease in degradation and the like and can be developed into a nuisanceless plant-sourced fungus inhibiting preparation.

The invention discloses application of tioconazole in preparation of a bactericide for preventing and controlling phytopathogenic fungi and bacteria. Through indoor agent sensitivity determination, itproves that the tioconazole has good inhibitory activity on main diseases of important grain crops including fusarium graminearum causing wheat scab, maize curvularia causing maize leaf spot, rhizoctonia cerealis causing sheath blight and magnaporthe grisea causing rice blast; moreover, the tioconazole has good inhibition activity on botrytis cinerea of gray mold causing main diseases of fruits and vegetables, Physalospora piricola Nose causing ring rot of apples and alternaria tenuissima causing leaf spot of the fruits and vegetables; and moreover, the tioconazole also has good inhibitory activity on soil-borne diseases which are extremely difficult to control in agricultural production, such as fusarium oxysporum causing fusarium wilt, and the antibacterial activity of the tioconazole is even better than that of carbendazim and tebuconazole which are traditional bactericides. The invention provides application of the tioconazole in prevention and control of important plant diseasesin agricultural production for the first time, and the tioconazole has wide market application prospect.