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Active ingredient of folium ficus caricae capable of inhibiting and killing plant pathogenic fungus as well as production method and application thereof

A technology for plant pathogenic fungi and fig leaves, applied in the field of plant pesticides, can solve problems such as non-target biological damage, environmental pollution, human health hazards, etc., and achieve effects from a wide range of sources

Inactive Publication Date: 2018-10-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The early pesticides used to inhibit and kill plant pathogenic fungi were all chemical pesticides, but with the continuous promotion and use of chemical pesticides, the harm it caused cannot be ignored
Extensive use of chemical pesticides will cause serious pollution to the environment, harm non-target organisms, and pesticide residues will also cause harm to human health

Method used

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  • Active ingredient of folium ficus caricae capable of inhibiting and killing plant pathogenic fungus as well as production method and application thereof
  • Active ingredient of folium ficus caricae capable of inhibiting and killing plant pathogenic fungus as well as production method and application thereof
  • Active ingredient of folium ficus caricae capable of inhibiting and killing plant pathogenic fungus as well as production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Fig leaf total extract,

[0025] (1) The dried fig leaves are crushed and passed through a 40-mesh sieve to obtain fig leaf powder.

[0026] (2) Get the 95% ethanol that is equivalent to about 7 times of the raw material quality (the general control range is 5-10 times) volume and extract at room temperature (the temperature is generally in the range of 18-24 ° C) for about 20h (the general control range is 12 -24h).

[0027] (3) Sonicate at about 50°C for 6 hours (the general control range is 4-8 hours). Use a Buchner funnel to filter under negative pressure to remove the residue to obtain the filtrate.

[0028] (4) the residue is further ultrasonically extracted for 6 hours (the general control range is 4-8h) with 95% ethanol equivalent to about 6 times (the general control range is 5-10 times) volume of the raw material quality, so that the activity in the fig leaves The ingredients are fully extracted, filtered with a Buchner funnel under negative p...

Embodiment 2

[0031] Embodiment 2: the preparation of n-hexane extract and antibacterial activity assay

[0032] (1) The dried fig leaves are crushed and passed through a 40-mesh sieve to obtain fig leaf powder.

[0033] (2) Get the 95% ethanol that is equivalent to about 7 times of the raw material quality (the general control range is 5-10 times) volume and extract at room temperature (the temperature is generally in the range of 18-24 ° C) for about 20h (the general control range is 12 -24h).

[0034] (3) Sonicate at about 50°C for 6 hours (the general control range is 4-8 hours). Use a Buchner funnel to filter under negative pressure to remove the residue to obtain the filtrate.

[0035](4) the residue is further ultrasonically extracted for 6 hours (the general control range is 4-8h) with 95% ethanol equivalent to about 6 times (the general control range is 5-10 times) volume of the raw material quality, so that the activity in the fig leaves The ingredients are fully extracted, fil...

Embodiment 3

[0039] Embodiment 3 ethyl acetate extract

[0040] (1) The dried fig leaves are crushed and passed through a 40-mesh sieve to obtain fig leaf powder.

[0041] (2) Get the 95% ethanol that is equivalent to about 7 times of the raw material quality (the general control range is 5-10 times) volume and extract at room temperature (the temperature is generally in the range of 18-24 ° C) for about 20h (the general control range is 12 -24h).

[0042] (3) Sonicate at about 50°C for 6 hours (the general control range is 4-8 hours). Use a Buchner funnel to filter under negative pressure to remove the residue to obtain the filtrate.

[0043] (4) the residue is further ultrasonically extracted for 6 hours (the general control range is 4-8h) with 95% ethanol equivalent to about 6 times (the general control range is 5-10 times) volume of the raw material quality, so that the activity in the fig leaves The ingredients are fully extracted, filtered with a Buchner funnel under negative pres...

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Abstract

The invention discloses an active ingredient of folium ficus caricae capable of inhibiting and killing plant pathogenic fungus as well as a preparation method and application thereof. The active ingredient is fine extract obtained by further extracting by adopting ethyl acetate or normal hexane as an extraction agent on the basis of extract obtained by adopting ethanol as an extraction solvent. The active ingredient of the folium ficus caricae capable of inhibiting and killing the plant pathogenic fungus can be used for preparing a preparation for inhibiting and killing the plant pathogenic fungus and mushroom pathogenic fungus, and particularly suitable for preparing a preparation for inhibiting and killing rape sclerotinia sclerotiorum, fusarium fungi, alternaria alternate, rhizopus oryzae and other pathogenic fungus. The active ingredient of the folium ficus caricae disclosed in the invention has the characteristics of wide raw material source, nontoxicity to the human body, harmlessness to crops, ease in degradation and the like and can be developed into a nuisanceless plant-sourced fungus inhibiting preparation.

Description

technical field [0001] The present invention relates to plant pesticide technology, more specifically, relates to a plant extract active ingredient for inhibiting and killing plant pathogenic fungi, its preparation method and application. Background technique [0002] Plant diseases are an important factor affecting agricultural production. Different pathogenic bacteria can cause diseases in a variety of crops, which has caused great losses to the agricultural economy. Fungi of the genus Fusarium can cause various diseases of a variety of plants, appear and develop rapidly in the world, and have brought great harm to the safety of global agricultural production. It is able to spend the year in the soil and infects a wide variety of plants, including cash crops, food crops, ornamentals and medicinal plants. It can cause a variety of diseases in plants, such as root rot, stem rot, flower rot, ear rot and other diseases. There are hundreds of host plants, infecting the vascula...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P3/00
Inventor 龙章富余晓瑞龙力陈次琼张福生胡宗悦方磊
Owner SICHUAN UNIV
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