440 results about "Rhizopus oryzae" patented technology

The invention relates to the field of microbial fermentation, in particular to a preparation method of a compound Daqu, so as to solve the problems of the traditional Daqu such as single nutritional components of raw materials, longer starter-making and brewing time, low utilization rate of grains, high cost and the like. The preparation method comprises the following steps: (1) treating the raw materials of the Daqu; (2) inoculating aspergillus niger 41254, aspergillus niger UV-11, monascus, mucor racemosus, actinomucor elegans, rhizopus oryzae, saccharomyces cerevisiae, candida drusei, acetobacter, bacillus subtilis and lactobacillus delbrueckii 6100 into a conical flask containing the raw materials of the Daqu for culturing, then expanding the culture and obtaining a mother culture; and(3) adopting uncooked materials for making a starter, inoculating the obtained mother culture and the raw materials of the Daqu into a solid fermentation device, keeping the temperature at 30 DEG C-33 DEG C, continuing for 30-33h, discharging the starter and finally carrying out low-temperature vacuum drying. The preparation method enriches the nutritional components in the raw materials and improves the quality of a brewed product; and a plurality of bacterial strain are artificially added, thereby improving the utilization rate of the grains, shortening the starter-making cycle, improving the yield of the brewed product and reducing the production cost.

The invention relates to a method for producing L-lactic acid. In the method, crop straw and Rhizopus oryzae serve as raw materials. The method particularly comprises the following operation steps: A. preparing straw saccharified liquid; B. adding auxiliary material to inoculate Rhizopus oryzae for fermentation; C repeatedly fermenting 25 batches; D. acidizing fermented clear liquid; E. ultrafiltration; F. nanofilteration; G. reverse osmosis; H. ion exchange; I. concentrating in vacuum to obtain the L-lactic acid product with the mass percentage concentration of 85%. The invention takes the straw hydrolysis saccharified liquid as the main raw material to prepare L-lactic acid, which obviously lowers the production cost of L-lactic acid; Rhizopus oryzae is adopted for once inoculation proliferation, thallus can be reused for several times to produce L-lactic acid, thus shortening L-lactic acid fermentation period, and obviously improving the fermentation strength of L-lactic acid; an advanced membrane separation and ion exchange integration technology is adopted to improve the lactic acid product quality; and the invention has simple process and strong continuity.

The present invention discloses a method for producing chitin/chitosan from the shell of silkworm chrysalis, fly larvina, which comprises: to break into pieces to progress the shell of silkworm chrysalis, fly larvina by dry or wet method; React the fine grits obtained with the fat enzyme and protein enzyme from micro-organism by a enzymolysis reaction in order to to eliminate the fat and protein contained in the chrysalis / larvina raw material; bleach using two-step method; Collect the materials after bleach and to wash, dry materials to obtain chitin product; Obtain chitosan by to circulate to cast off Acetyl reaction in a Rhizopus oryzae full cell immobilization biology reaction vessel; Obtain height hydrosoluble shel oligosaccharide by liquid submerged fermentation chitosan using acid formers. The present invention improves the quality of the product and the efficiency of production; reduces greatly the energy cost, the usage of acid, alkali, oxidant and other harmful chemistry thing and the discharge of effluent, waste residue; produces byproducts of high value at the same time; utilizes the resources integratedly; and increases the benefits of production.

A (Rhizopus Oryzae)ME-F11, its mutation screening method and method for fermenting fumaric acid are disclosed. The preserving code is CCTCC NO: M 207066. The fumaric acid concentration reaches to 90-120g/L, total yield reaches to 90-95 wt% . It's simple and can be used for industrial production.

Fermented soybean foods rich in gamma-aminobutyric acid are produced by fermentation of soybean using Tempe molds. Fermented soybean foods rich in gamma-aminobutyric acid and free amino acids are produced by fermentation of soybean using Tempe molds with anaerobic treatment. Fermented cereal foods rich in gamma-aminobutyric acid and free amino acids are also produced by fermentation of cereals with anaerobic treatment. Rhizopus oligosporus and Rhizopus oryzae are favorably used as the Tempe mold and Koji molds.

The invention belongs to the technical field of white-wine brewing processes. In order to solve the problems of low wine production rate and ethyl-acetate/ethyl-lactate rate in summer in the solid fermentation process of the traditional faint-scent white wine, the invention provides a production method for brewing the faint-scent white wine by fermentation of multi-strain enhanced yeasts. The production method comprises the steps of utilizing fresh fermented grains of fen wine to screen wine-brewing yeasts with high ester yield, rhizopus oryzae for producing amylase, acid-resistant and alcohol-resistant monascus fulginosus and high-temperature-resistant bacillus subtilis with the activities of protease and amylase and prepare wine yeast for producing the wine-brewing yeast, bran koji for producing the rhizopus oryzae, rice koji for producing the monascus fulginosus and bacteria solution for producing the bacillus subtilis, then adding into grains together with the yeast for production by a certain proportion, putting into a jar for fermentation and thus obtaining the faint-scent white wine. The production method provided by the invention has the beneficial effects that the cost is reduced; the obtained wine is high in ethyl acetate/ethyl lactate rate, total-acid content and wine production rate and strong in ester fragrance and has faint scent, preserved fragrance and sauce feel, and tastes sourly and coolly with mellowness and softness, and the difficulties of low and uncoordinated wine fragrance, lack of mellowness and short aftertaste due to low ethyl-acetate/ethyl-lactate rate and low acidity of the faint-scent white wine are solved.

The invention relates to a method for preparing a germinated brown rice ferment drink. Germinated brown rice is processed in an Aspergillus oryzae and Rhizopus oryzae mixed culture manner, is decomposed by using the enzyme system complementarity, and is inoculated with lactic acid bacteria to carry out fermentation. The prepared germinated brown rice ferment drink is rich in organic acids, variousfree amino acids, soluble dietary fibers, and active components which are gamma-aminobutyric acid and SOD, and has the advantages of sour-sweet mouthfeel, convenience in drinking, no additives, excellent nutrition and healthcare effects, great improvement of the raw material utilization rate in a two-stage fermentation process, and great solving of resource wastes of existing brown rice having bad mouthfeel.

The invention discloses a microbial compound preparation, a preparation method and a method for treating kitchen garbage. The method for treating kitchen garbage is to separate out mixed bacteria of bacteria and fungi with a certain ratio from the natural world and fermented food, and then prepare the mixed bacteria preparation to effectively treat the kitchen garbage. The preparation comprises the mixed bacteria including various bacteria, actinomycetes, yeast and mycetes. The preparation is characterized in that the mixed bacteria further comprise bacillus subtilis, bacillus licheniformis, high-temperature monad, streptomycete, lactobacillus plantarum, lactobacillus acidophilus, brewer's yeast, pichia pastoris, aroma-producing yeast, rhizopus chinensis, rhizopus oryzae and mucor indicus. The preparation is good for treating the kitchen garbage, non-toxic, harmless, and of genetic stability.

The present invention discloses a preparation method of high-SOD-activity fruit and vegetable enzyme powder. The enzyme powder is prepared by squeezing Chinese watermelon flesh and apple flesh into pulp, respectively fermenting filtrate using saccharomyces cerevisiae and hansenula anomala and streptococcus thermophilus and lactobacillus plantarum, fermenting filter residues with rhizopus oryzae suspension, mixing the three fermentation liquor, conducting secondary fermentation on the mixture, and finally spray-drying the mixture. The prepared enzyme powder is good in mouthfeel and flavor, high in SOD activity and high in lactic acid bacteria content.

The invention provides a fermentation technology of low-salt fermented bean curd. The fermentation technology comprises the following steps: base preparing, inoculating and cultivating, spraying, pre-fermenting, hair-rubbing and pickling, and soup-filling. According to the technology, the bases are prepared by steam-boiling of soybean slurry; a mixed spore suspension of hair mould and Rhizopus oryzae is utilized in grafting; table salt is used for hair-rubbing and curing of the bean-curd white bases, wherein the weight of the table salt is 10-14% that of the bean-curd white bases; and alcohol with the alcoholicity being 16-20% is used for soup-filling. The fermented bean curd is prepared under multi-aspect coordinated actions, so that the prepared final products are small in individual difference and batch difference. The prepared low-salt fermented bean curd is good in appearance and taste and is not prone to deterioration.

The invention discloses a method for treating heavy metal wastewater. The method comprises the following steps: (1) immobilizing mixed bacteria of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae, collected after mixed fermentation culture, into immobilized cell spheres, wherein the appearances of the immobilized cell spheres are spherical, and the interiors of the immobilized cell spheres are of a porous mesh structure; (2) adding a rhizopus oryzae spore suspension liquid into starch bearing material hydrolysate as a culture medium at the pH of 3-6, carrying out shake cultivation for 24-48 hours, filtering to collect rhizopus oryzae mycelium spheres with the diameters of 50-100 microns; (3) filling a biological adsorber with the immobilized cell spheres and the rhizopus oryzae mycelium spheres, wherein the biological adsorber is formed by virtue of series connection of multiple adsorption columns; and (4) after regulating the initial heavy metal ion concentration of heavy metal wastewater to be less than or equal to 800mg/L and the pH value to be 5-7, enabling heavy metal wastewater to flow through the biological adsorber, wherein the hydraulic retention time in each adsorption column is 0.5h-1h. According to the method, different heavy metals (Cd<2+>, Hg<2+>, Cu<2+>, Zn<2+>, Ni<2+>, Ag<2+> and Pb<2+>) can be well adsorbed by virtue of different types of microbial live bacteria under different adsorption mechanisms.

The invention provides a method for preparing a feed from common andrographis herb dregs by virtue of fermentation. The method comprises the following steps: (1) pretreatment of dregs, namely drying the dregs until the moisture content is 2-5wt% and grinding until the power granularity is within the range of 2.5-10 meshes; (2) preparation of a dreg culture medium, namely mixing 50 parts of dregs with bran in the ratio of 1: 1 to 2: 1, 0.2-0.6 part of corn flour, 0.2-0.6 part of ammonium chloride and 0.1-0.3 part of monopotassium phosphate in 25-50 parts of sterile water, pouring the obtained mixed liquid into a conical flask and mixing evenly to obtain natural pH, and sterilizing at 121 DEG C under high pressure for 20 minutes; (3) dreg fermentation, namely inoculating 10-30mL of the second-level seeds of rhizopus oryzae strain or bacillus subtilis strain into each 100g of dreg culture medium, fermenting at 26-30 DEG C for 48-96 hours, and shaking the culture medium evenly every 12 hours; (4) product treatment, namely drying the culture medium obtained by fermentation, and grinding and sieving by use of a sieve of 2-4 meshes, thereby obtaining the fermented feed product. The method is simple in process, short in time, low in material consumption, advantageous for industrial pilot scale-up, and relatively high in yield.

The invention belongs to the technical field of biological engineering, which relates to a method for separating and extracting boletic acid in situ in the process of utilizing rhizopus oryzae fermentation to prepare boletic acid. The invention aims at the special physicochemical properties of boletic acid, when rhizopus oryzae free cell is utilized to ferment, the invention adopts a hollow fiber ultrafilter membrane, concentration by nanofiltration and ion exchange adsorption coupling technique to separate and extract boletic acid. The operation condition of the method is mild, separation selectivity is strong, activity loss of somatic cell is small, fermentation efficiency of rhizopus oryzae is effectively improved, process flow of utilizing a biological method to synthesize boletic acid is reduced, and meanwhile, the invention utilizes a nanofiltration device to concentrate fermentation liquor in the process of reacting and separating, which greatly improves adsorption capacity of ion exchange resin to boletic acid, and material consumption and energy consumption are relatively lower, and waste and secondary pollutants which are produced are relative little. The reacting and separating process of the method are fast, complete, and operation stability is excellent, the method is a high-effective and novel mass transfer separation coupling strengthening technique, which has wider industrialized production prospect.

The invention discloses a Saccharomyces cerevisiae genetic engineering strain for producing fumaric acid as well as a construction method and application thereof, belonging to the field of genetic engineering. The construction method provided by the invention comprises the following steps: cloning key genes containing dehydrogenase genes (RoMDH) and fumarase genes (RoFUM1) in the accumulation path of the fumaric acid from the Rhizopus oryzae NRRL1526 capable of accumulating a large amount of fumaric acid, and converting the Saccharomyces cerevisiae BMA64 by connecting to the plasmid pY26TEF/GPD capable of realizing high-copy two-way expression in the Saccharomyces cerevisiae to obtain the Saccharomyces cerevisiae genetic engineering strain for producing fumaric acid. The Saccharomyces cerevisiae genetic engineering strain disclosed by the invention can accumulate the fumaric acid in the cytoplasm, the fermentation method is simple, the yield can reach 26.2mg/L, and the invention establishes a basis for industrialization of Saccharomyces cerevisiae for producing fumaric acid in metabolic engineering construction.

The invention relates to a high-efficiency engineering bacteria agent and a method for producing an active biological organic fertilizer and belongs to the technical field of biology. The bacteria agent consists of the following strains in parts by weight: 50 to 60 parts of enzyme producing bacteria, 6 to 10 parts of bacillus thuringiensis and 10 to 15 parts of bacillus subtilis, the enzyme producing bacteria consist of the following strains in parts by weight: 1 to 3 parts of umbrella mucor, 1 to 3 parts of rhizopus oryzae, 3 to 5 parts of bacillus licheniformis and 3 to 5 parts of Pichia pastoris. The high-efficiency engineering bacteria agent provided by the invention can realize high-efficiency conversion of harmlessly treated kitchen waste to obtain the high-efficiency active biological organic fertilizer. According to determination, the organic fertilizer satisfies relevant indicators of organic fertilizers in agricultural standard NT 525-2012 on organic fertilizers, can effectively increase the number of soil organic matters and effective microbial communities, can activate soil, can promote the absorption of plant root systems to nutrients, can improve the yield and quality of crops, and is a high-grade fertilizer for producing pollution-free and organic agricultural products.

The invention relates to a method for preparing and regenerating a rhizopus oryzae protoplast for producing L-lactic acid at high yield, comprising the following steps of: A. preparing a rhizopus oryzae spore suspension; B. preparing a seed culture liquid; C. preparing a hypha suspension; D. enzymolyzing a cell wall; E. separating a protoplast; and F. regenerating the protoplast. The rhizopus oryzae protoplast prepared with the method disclosed by the invention is in an approximately-circular shape and has higher activity; the preparation quantity and the regeneration rate of the rhizopus oryzae protoplast can respectively reach 2.74*10<6>/mL and 6.8 percent to the maximum extent. The method is easy to operate, has low requirements for the equipment, and provides a technical reference for preparing and regenerating the rhizopus oryzae protoplast. By using the method disclosed by the invention, the protoplast can be quickly prepared with short regeneration period and high regeneration speed; and the preparation efficiency of the protoplast can be effectively improved. The protoplast prepared with the method provided by the invention completely meets the requirement for fusing the protoplast. The invention provides an operation method with practical significance for preparing and regenerating the protoplast.

Provided is a Rhizopus oryzae TY GF1 bacterial strain. The preservation date is December 20th in 2010 and the preservation registration number is CGMCC No.4490. The application of the Rhizopus oryzae TY GF1 bacterial strain in degrading high-concentration oil in catering waste water effectively is further provided.

The invention belongs to the technical field of energy and relates to a method for producing biodiesel by microalgae fermentation. The method comprises the following steps of 1, microalgae culture, 2, fermentation of the cultured microalgae by trichoderma harzianum and rhizopus oryzae as zymophyte and a fermentation culture medium containing 20-30g/L of microalgae, 3-5g/L of sodium citrate, 1.5-2.5g/L of o-toluidine, 10-15g/L of sodium chloride, 0.5-1g/L of potassium dihydrogen phosphate and 3-5g/L of magnesium sulfate, 3, extraction of grease in the fermentation products, and 4, enzymic catalytic reaction of the grease. The biodiesel obtained by the microalgae fermentation has a fast oil production rate and a low production cost.