High-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and preparation method thereof

A straw decomposing agent and three-element enzyme technology, which can be used in biochemical equipment and methods, preparation of organic fertilizers, methods based on microorganisms, etc., and can solve problems such as lack of

Inactive Publication Date: 2018-07-24
三门峡龙飞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology lacks a "three-enzyme" system that can effectively decompose straw and have obvious synergistic effects

Method used

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  • High-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and preparation method thereof
  • High-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and preparation method thereof
  • High-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0064] Preparation Example 1: Preparation of high-yield "three-suzyme" straw decomposing agent of the present invention

[0065] Step A: Preparation of high-yield cellulase Bacillus subtilis fermentation broth

[0066] Activation of high cellulase-producing Bacillus subtilis strain BS-05: use an inoculation loop to pick a ring of Bacillus subtilis strains and inoculate them into a slant test tube of the seed medium, activate at a temperature of 37°C for 18 hours; use an inoculation loop to pick two Inoculate the ring-activated Bacillus subtilis strain into 150ml shake flask culture medium, culture in a constant temperature shaker at a temperature of 37°C and a rotation speed of 120r / min for 24h; when the temperature of the culture solution drops to 35°C, follow the fermentation culture Inoculate the shake flask culture into the fermentation medium with an inoculation amount of 5% based on weight, and ferment at a temperature of 35°C, an air flow of 1:1, a tank pressure of 0.05...

preparation Embodiment 2

[0088] Preparation Example 2: Straw decomposing agent containing Bacillus subtilis fermentation liquid and Rhizopus oryzae fermentation product

[0089] The preparation method of Bacillus subtilis fermentation broth and Rhizopus oryzae fermentation product is the same as A step and B step of Preparation Example 1.

[0090] First-level expansion:

[0091] Mix 80 parts of bran with appropriate amount of water and sterilize in a steamer for 1 hour. After sterilization, take it out and let it cool; wait until the temperature drops to 35°C, then take 1.5 parts of brown sugar and add appropriate amount of water to dissolve, and 8 parts of Bacillus subtilis Bacillus fermentation liquid and 12 parts of Rhizopus oryzae fermented product were sprinkled on the bran and stirred evenly, and the water content was kept at 35% to carry out fermentation and cultivation for 48 hours to obtain the first-level expansion culture strain.

[0092] Secondary expansion:

[0093] Add 500 parts of ric...

preparation Embodiment 3

[0094] Preparation Example 3: Straw decomposing agent containing fermented liquid of Bacillus subtilis and fermented liquid of white rot fungi

[0095] The preparation method of Bacillus subtilis fermentation liquid and white rot fungus fermentation liquid is the same as Step A and Step C of Preparation Example 1.

[0096] First-level expansion:

[0097] Mix 90 parts of bran with appropriate amount of water and sterilize in a steamer for 1 hour, take it out and let it cool; wait until the temperature drops to 40°C, then take 2 parts of brown sugar and add appropriate amount of water to dissolve, and 7 parts of Bacillus subtilis Fermentation broth and 13 parts of white rot fungus fermentation broth were sprinkled on the bran and stirred evenly, and the water was kept at 45% to carry out fermentation and cultivation for 48 hours to obtain the first-level expansion culture strain.

[0098] Secondary expansion:

[0099] Add 470 parts of rice bran, 600 parts of bran, 20 parts of ...

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Abstract

The invention relates to a high-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive and a preparation method thereof. The high-yield 'cellulose, hemicelluloses and ligninenzyme' straw decomposition additive comprises the following preparation raw materials: a cellulose, hemicelluloses and lignin enzyme mixture, plant materials, sugar and urea, wherein the cellulose, hemicelluloses and lignin enzyme mixture consists of cellulase bacillus subtilis fermentation liquor, a rhizopus oryzae fermentation substance and ligninase white-rot fungi fermentation liquor; the weight ratio of the cellulase bacillus subtilis fermentation liquor to the rhizopus oryzae fermentation substance to the ligninase white-rot fungi fermentation liquor is 3-7 to 5-10 to 5-10. By adoptingthe high-yield 'cellulose, hemicelluloses and lignin enzyme' straw decomposition additive provided by the invention, different bacterial florae can harmoniously coexist and be mutually promoted, an effect of complementary advantages can be achieved, the effective viable count can be increased, the enzyme yield can be increased, cellulose, hemicelluloses and lignin in crop straw can be rapidly andeffectively decomposed, and rapid treatment and circulation of straw biomass resources can be achieved.

Description

technical field [0001] The invention belongs to the technical field of crop straw treatment. More specifically, the present invention relates to a high-yield "three-suzyme" straw decomposing agent and a preparation method thereof. Background technique [0002] Crop straw contains a large amount of cellulose, hemicellulose, and lignin. It is the most abundant biomass resource in the world and is the core of the current transformation and utilization of biomass resources. According to incomplete statistics, my country currently produces more than 900 million tons of various types of straw, and the utilization rate is less than 50%, of which only 6-7% are returned directly to the field or through the belly. The unused straw has caused great harm to the environment. negative effect. It is one of the effective measures for resource recycling to develop microbial agents that can promote straw decomposition, realize rapid decomposition of straw and return it to the field, recycle ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F15/00C05F17/00C12R1/125C12R1/845C12R1/645
CPCC05F11/00C05F17/00C12N1/14C12N1/20C05F11/08Y02W30/40
Inventor 易卿赵跃民赵跃文赵洁谢松任寸娟秦中辉张盼
Owner 三门峡龙飞生物工程有限公司
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