89results about How to "High effective count" patented technology

Disclosed are a tobacco composite microbial fertilizer and its preparation method. The tobacco composite microbial fertilizer contains an active functional bacteria ingredient, an organic fertilizer ingredient and an inorganic composite fertilizer ingredient, wherein the active functional bacteria ingredient contains phosphate solubilizing bacteria-Bacillus megaterium powder, potassium bacteria-Bacillus mucilaginosus powder, tobacco growth-promoting rhizobacteria-Bacillus amyloliquefaciens powder and tobacco endophytic antagonistic bacteria-Brevibacillus laterosporus powder; and the organic fertilizer ingredient contains decomposed manure, a rapeseed cake fertilizer and straws. The invention also relates to a preparation method of the tobacco composite microbial fertilizer. The fertilizerprovided by the invention has advantages of an organic fertilizer, an inorganic fertilizer and a microbial fertilizer. According to the invention, utilization rate of the fertilizer can be raised, soil organic matters can be increased, tobacco plant rhizosphere microbial proliferation can be promoted, soil physical and chemical property and biological activity can be improved, comprehensive and balanced nutrients can be provided for the early growth of tobacco seedling, the occurrence of soil borne disease can be decreased, and flue-cured tobacco output and quality can be substantially improved.

The invention discloses a method to prepare leaven of fertilizer and application, which is characterized by the following: allocating raw material with mass ratio at 10-80 wt industrial residue of ferment, 5-30 wt straw, 5-30 wt cake meal, 10-30 wt bran or rice bran, 1.5-2 wt aquacare, 0.5-1 wt NaCl and or amylaceum, and 1-30 wt brown ball Azotobacter (Azotobacter chroococcum), 1-30 wt bacillus megaterium (Bacillus megaterium), 1-30 wt bacillus licheniformis (Bacillus licheniformis), 1-30 wt plant lactobacillus (Lactobacillus plantarum) and 1-30 wt 1-5 strain of saccharomycopsis fibuligera (Saccharomycopsis fibuligera); producing the product.

The invention provides a microbial fertilizer manufacturing method. Antibiotic fungus residue serves as a base material, and compound microorganisms are adopted to perform solid fermentation on materials, wherein the materials comprise waste rich in plant fiber and the antibiotic fungus residue. The compound microorganisms comprise cellulose decomposition bacteria and one or more of bacillus natto, saccharomyces cereviseae and lactobacillus plantarum. According to the microbial fertilizer manufacturing method, the problem that it is difficult to treat the fermented antibiotic fungus residue in a pharmaceutical factory at present can be solved. Furthermore, beneficial ingredients in the antibiotic fungus residue are can be utilized and converted into a microbial fertilizer, so as to solve the problem that at present, the soil hardening occurs and the fertility declines.

The invention discloses a method for producing viable bacillus subtilis by way of solid fermentation, which includes the following steps: the ACCC03120 bacillus subtilis strain is chosen, cultured by utilizing solid fermentation under the temperature of 30 DEG C to 35 DEG C for 48 to 72 hours, then naturally aired for 35 to 45 hours and ground to pass a 80-mesh sieve, and thereby a finished solidfermentation product containing the viable bacillus subtilis is prepared. The material cost of the method is low, the production process is simple, large equipment is not needed, moreover, the effective viable count of the bacillus subtilis in the product is high and 60 billion per gram of the dry fermentation product, and the demand on a great deal of microorganism fertilizer can be satisfied.

The invention provides a microorganism bactericide capable of reinforcing plants to restore fields polluted by arsenic and preparation and application methods thereof, belonging to the technical field of environment restoration engineering. The strain utilized by the bactericide is Agrobacteriumsp. C13. The bactericide adopts a composite carrier which comprises turfy soil, saw dust, rice hulls and active carbon with the mass percentage being 30:25-30:25-30:15; the microorganism fermentation liquor is mixed with the composite carrier according to the mass ratio of 0.5-1:1; and the adsorption amount of the microorganisms of the composite carrier reaches 109-1012 per gram. The prepared bactericide is applied to the arsenic-polluted fields restored by ciliate desert-grass according to the use amount of 5g/kg, the biomass liveweight of the overground part of the restored plants is improved by 16-17%, and the removal rate of arsenic in the fields is improved by 40-41% in comparison. The carrier of the bactericide in the invention has rich raw materials, simple preparation method and easy realization of large-scale production.

The invention discloses a production method of a probiotic soil conditioner. The production method comprises the steps of: (1) respectively inoculating huge bacillus, bacillus subtilis and bacillus licheniformis into a seed cultivation medium to cultivate and obtaining respective seed liquid; (2) adding the cultivation medium for production into a fermentation tank to sterilize and cool; and (3) inoculating the bacillus seed liquid into the fermentation tank to cultivate under an oxygen-leading agitating condition, inoculating the bacillus subtilis seed liquid and bacillus licheniformis seed liquid to cultivate, and obtaining the probiotic soil conditioner. The invention also discloses the probiotic soil conditioner obtained by the method. The method disclosed by the invention is scientific and reasonable, simple and feasible in production process and strong in industrialization implementation, and has an important significance to the development of agricultural production.

The invention discloses a high-temperature kitchen waste degradation microbial agent and a preparing and using method thereof. A microbial strain comprises, by weight, 15-30 parts of nuby land anoxybacillus, 10-25 parts of liquefaction thermophilic bacillus, 20-40 parts of hot nitrogen bacillus and 25-50 parts of geobacillus stearothermophilus. Compared with the prior art, the high-temperature kitchen waste degradation microbial agent has the beneficial effects that as for kitchen waste with different integrants, reasonable matching on single bacterium powder can be carried out, and single bacterial strains are cooperated for degrading the kitchen waste in a collaborative mode; the number of effective viable bacteria in the single bacterium powder in the composite microbial agent is large, and when the microbial agent is used for degrading the kitchen waste, only a small number of microbial agents is needed for sufficiently and rapidly degrading the kitchen waste; components in the microbial agent are high-temperature cultures, the optimum fermentation temperature ranges from 55 DEG C to 65 DEG C, pathogenic bacteria such as escherichia coli and staphylococcus aureus in the kitchen waste can be effectively inactivated in the fermentation process, and the biosecurity is high.

The invention provides a rapid odor-free harmless livestock excrement efficient decomposition agent and a fermentation method thereof. The decomposition agent is prepared from, by mass, microbial strain components and a carrier component, wherein the microbial strain components comprise 10-15% of bacillus subtilis, 10-15% of bacillus licheniformis, 5-15% of yeast, 3-8% of aspergillus niger, 10-20% of lactic acid producing lactobacillus acidophilus and 10-20% of lactic acid producing lactobacillus plantarum, and the carrier component is 10-20% of maltodextrin. The decomposition agent is mainly prepared from spore bacteria of various feed grades, other microbial bacteria and the carrier maltodextrin, can rapidly decompose and ferment livestock excrement into odor-free substances within 3-7 days and then form the safe harmless product through accumulated fermentation to kill pathogenic bacteria, parasites, weed seeds and others in the product, and the product can serve as organic fertilizer and influence the germination index of plants.

The invention provides a special bioorganic fertilizer for leafy vegetables. The special bioorganic fertilizer is prepared from, by weight, 40-80 parts of organic residues, 10-15 parts of nitrogen-phosphorus-potassium fertilizers, 0.5-1 part of microbial agent and 5-10 parts of additives. The microbial agent is composed of bacillus licheniformis, paecilomyces lilacinus and bacillus amyloliquefaciens, living bacterium number ratio of the bacillus licheniformis, the paecilomyces lilacinus to the bacillus amyloliquefaciens is 1-85:1-200:10-100, and living bacterium count of the microbial agent is 0.23billion/g. The organic residues can be effectively degraded by the microbial agent to provide survival nutrition for microorganisms, so that the survival rate of the microorganisms is increased, and shelf life is prolonged. In addition, by a synergistic effect of the bacillus licheniformis, the paecilomyces lilacinus and the bacillus amyloliquefaciens, a soil root system environment can be improved to further improve crop quality.

The invention discloses a method for producing a biological bactericide by using paper-making wastewater and belongs to the technical field of the production of biological bactericides. According to the method, pollutions caused by paper-making black liquid can be treated while a high-quality biological bactericide is produced, and circulating economic benefits for the paper-making industry are implemented. The method comprises the following steps of: extracting the paper-making black liquid produced by pulping by using potassium carbonate; adding 100 parts by weight of the black liquid into a fermenting device, adding 1-5 parts by weight of sugar or 2-10 parts by weight of molasses, adding 0-20 parts by weight of humic acid or 0-5 parts by weight of defluorinated phosphoric acid, and adjusting the pH value to less than or equal to 8; pouring ozone and sterilizing for about 2 hours; adding 1-3 parts by weight of an anaerobic and facultative anaerobic compound biological bactericide into the fermenting device after the sterilization is finished for one hour, and expanding propagation for 72 hours; and adding aerobiotic bactericide which is 0.1-10% of the weight of the paper-making black liquid to compound till the living bacterium quantity of the produced biological bactericide is greater than or equal to 2 billion/ml. The method for producing the biological bactericide by using paper-making wastewater is low in cost, simple to operate, high in number of living bacterium in the product, rich in nutrient substances such as phosphorus potassium humate and high in quality, turns the paper-making liquid into wealth and realizes the effective utilization of resources, so that the problem of environment pollution is solved, good economic benefits are brought while high cost for environmental protection is saved, and the possibility for the popularization of a potassium carbonate pulping process is provided.

The invention relates to a preparation method of a composite solid-state probiotic agent including phellinus linteus. The method comprises the following steps of inoculating phellinus linteus seed fluid to solid-state fermentation culture mediums, performing fermentation, and degrading cellulose and hemicellulose type substances for producing monosaccharide; and then sequentially adding after fermentation bacterial fluid for a plurality of times, performing fermentation to obtain fermentation products, and then drying the fermentation products so as to obtain the composite solid-state probiotic agent. According to the preparation method disclosed by the invention, stagewise solid-state fermentation is performed according to requirement differences of different probiotics for nutrient substances, so that the culture mediums can be efficiently decomposed; phellinus linteus grows through solid-state substrates to produce active polysaccharide, and besides, the cellulose and hemicellulose type substances are degraded to generate the monosaccharide; and the solid-state probiotic agent has special paste fragrance, contains fungus polysaccharide, is high in effective viable count, is high in the content of organic nitrogen, and has the efficacy of adjusting the flora in the intestinal tracts of animals, enhancing immunity, increasing the utilization rate of the solid-state substrates, reducing the fetor of excrement of livestock and improving environment.

The invention discloses a green and pollution-free organic fertilizer, comprising the following raw materials in parts by weight: 25-36 parts of maize meal, 22-36 parts of rice bran, 20-30 parts of wheat bran, 14-20 parts of soybean cake powder, 12-16 parts of wormcast, 8-12 parts of urea, 6-8 parts of turf, 6-8 parts of perlite, 6-7 parts of pine needle meal, 5-6 parts of tobacco powder, 4-5 parts of powdered rock phosphate, 3-4 parts of brown sugar, 2-3 parts of actinomycetes, 2-3 parts of enzyme microorganism and a proper amount of water. According to the fertilizer disclosed by the invention, the maize meal, the rice bran, the wheat bran and the like are adopted as main raw materials, the soybean cake powder, the wormcast, the urea, the tuft and the like are adopted as auxiliary materials, the organic fertilizer is free of pollution to the natural environment and the soil when comprehensive nutrients are supplied to crops, the effective living bacteria in the fertilizer are high in quantity by combination of the added enzyme microorganism and actinomycetes, various animal and plant residues in the soil can be quickly decomposed, chemical components such as pesticide residues and chemical fertilizers, and refractory minerals also can be decomposed, the pollution on the soil and agricultural products caused by the chemical fertilizers and pesticides can be reduced, the high-yield, high-quality and pollution-free effects are achieved, and the organic fertilizer accords with the sustainable development of agriculture, and has good popularization and application value.

The invention provides a soil conditioner for improving and remediating soil and application thereof. The soil conditioner is prepared from decomposed organic material, phosphogypsum and bacillus amyloliquefaciens, wherein the mass ratio of the decomposed organic material in the soil conditioner is not lower than 65.0%, the mass ratio of phosphogypsum is 25%-30%, and the content of bacillus amyloliquefaciens is not lower than 2*10<8> CFU/g. The soil conditioner has an obvious soil conditioning and remediating function and can effectively promote formation of soil granular structures, improve the soil buffer capacity and the water and nutrient retention capacity, enrich beneficial microbial florae in soil, improve soil bioactivity, improve soil acidity and alkalinity and significantly condition and remediate greenhouse soil.

The invention discloses an organic fertilizer production method, which comprises: crushing flue-cured tobacco straw, and sterilizing for 30-45 min at a temperature of 90-100 DEG C to obtain a sterilized material; mixing a microbial agent into the sterilized material to obtain a mixed material, wherein the adding amount of the microbial agent is 0.07-0.13% of the mass of the sterilized material, and the microbial agent comprises Bacillus subtilis, Bacillus Licheniformis, saccharomycetes and Aspergillus Niger; and adding effective flora into the primary fermentation material, and carrying out secondary fermentation to obtain the organic fertilizer. According to the present invention, with the method, more than 95% of pathogenic bacteria can be reduced through early-stage high-temperature sterilization pretreatment, and primary fermentation and secondary fermentation propagation are carried out step by step so as to avoid the effective flora inactivation caused by the too high primary fermentation temperature, such that the effective flora can be sufficiently propagated, and the effective viable count can be increased.

The invention discloses a soil modifier prepared by using phosphogypsum and a preparation method thereof. The soil modifier is prepared from, 30-40 parts of the phosphogypsum, 60-80 parts of limewater, 20-30 parts of eupatorium adenophorum, 3-7 parts of coconut powder, 12-20 parts of rapeseed dregs, 6-10 parts of humic acid, 8-16 parts of pig manure, 2-3 parts of compound amino acid liquid, 5-9 parts of plant ash, 0.1-0.5 part of zinc sulfate monohydrate, 2-4 parts of polyacrylamide, 8-14 parts of ammonium tribasic phosphate and 1-2 parts of complex microbial community. According to soil modifier, by using the phosphogypsum to prepare the soil modifier, the environmental pollution problem brought by the accumulating of of the phosphogypsum can be effectively solved, and the prepared soil modifier can obtain better economic benefit.

The invention provides a microbiological enveloping compound fertilizer capable of improving a nematode killing efficiency. The microbiological enveloping compound fertilizer consists of a three-layer structure, wherein the inner part of the three-layer structure is a compound fertilizer core layer, the middle of the three-layer structure is an oily enveloping substance, and the outer part of the three-layer structure is bacterium powder with an insect killing function, the weight ratio of the compound fertilizer core layer to the oily enveloping substances to the bacterium powder with the insect killing function is (300-600) to(1-5) to (1-10), the oily enveloping substances comprise liquid organic carbon of which the content of organic matters is more than 40%, and the number of effective viable bacteria of the bacterium powder with the insect killing function is larger than or equal to 5 hundred million/g. The microbiological enveloping compound fertilizer disclosed by the invention can realize the purpose of increasing the rate of crops for preventing and treating nematode.

The invention relates to the field of microbial fertilizer, and specifically discloses a low temperature anti-biological inoculant, a solid state fermentation method and applications thereof. The effective component of the low temperature anti-bacterial inoculant is pseudomonas VT-B5, and the effective viable count is 1.0*10<10> cfu/g or more. The solid state fermentation method comprises the following steps: (1) activating bacterial strains; (2) preparing a primary seed liquid; (3) preparing a secondary seed liquid; (4) carrying out solid state fermentation; (5) drying the fermentation product, adding a stabilizing agent and auxiliary agents, grinding, and packaging to obtain the low temperature anti-bacterial inoculant. The provided low temperature anti-bacterial inoculant has a good performance and high quality, can prominently improve the solid property, stress resistance and disease resistance of plants, and crop quality, and is capable of increasing the output. The production technology using a solid state fermentation method has the characteristics of low production cost, stableness, and environment-friendliness.

The invention provides a concentrated microbial agent of bacillus mucilaginosus and a preparation method and application thereof, and relates to the technical field of preparation of bacterial fertilizers. The effective viable count of the bacillus mucilaginosus in the concentrated microbial agent is greater than or equal to 1 billion/mL; the bacillus mucilaginosus is a bacillus mucilaginosus Sanju No.01 strain, and the preservation number of the bacillus mucilaginosus Sanju No.01 strain is CGMCC No.3995. The content of the bacillus mucilaginosus in the concentrated microbial agent is far higher than that required by technical indexes of Agricultural Microbial Agents (GB20287-2006), and the use effect on crops is more remarkable; the process flow is simple, the operability is high, the effective viable count is high, the nutrients are rich, the photosynthesis efficiency of the crops is improved accordingly, the stress resistance is improved, the growth of the crops is facilitated, finally, the quality of the crops is improved, and the yield of the crops is increased.

The invention discloses a fulvic acid microbial agent and a preparation method thereof. The fulvic acid microbial agent comprises the following raw materials: fulvic acid powder, a mixed microbial agent protective agent and a biological agent, wherein the mixed microbial agent protective agent comprises the following raw materials: bran, molasses, sodium benzoate and carbendazim. The fulvic acid microbial agent disclosed by the invention has the beneficial effects that the mixed microbial agent protective agent is added into the biological agent, so that the number of effective viable bacteriaof a microbial product is increased, and the storage life of the microbial product is prolonged. The mixed microbial agent protective agent adopted in the fulvic acid microbial agent is composed of aprotective agent (molasses and bran) and a bacteriostatic agent (sodium benzoate and carbendazim), so that infectious microbes in the biological agent can be effectively prevented from growing whilethe biological agent is protected. Therefore, the number of effective viable bacteria in the microbial product is improved, and the storage life is prolonged.