Microbial agent and preparing method thereof

The technology of a microbial agent and production method, which is applied in the field of microbial fermentation, can solve the problems of low effective count of microbial agents, high content of heavy metals in microbial agents, and high rate of miscellaneous bacteria in microbial agents, so as to increase the number of effective viable cells , low heavy metal content, and low bacteria rate

Active Publication Date: 2016-03-30
SHANDONG HETIANWANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) The bacterial content of the microbial agent is low; (2) The effective number of viable bacteria of the microbial agent is low; (3) The bacterial rate of the microbial agent is high; (4) The heavy metal content of the microbial agent is high; (5) The shelf life of microbial agents is short

Method used

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  • Microbial agent and preparing method thereof
  • Microbial agent and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 A kind of production method of microbial bacterial agent

[0032] Step 1, strain selection

[0033] The bacteria used include:

[0034] The preservation number of Bacillus mucilaginosus is ACCC10013;

[0035] The preservation number of Bacillus subtilis is ACCC01175;

[0036] The preservation number of Bacillus licheniformis is ACCC11091;

[0037] All the strains mentioned above were purchased from the market.

[0038] Step 2. Strain activation and expansion

[0039] (1) Activation and expansion of jelly-like bacillus

[0040] Inoculate the jelly-like Bacillus strain on a plate medium, and culture it at 30° C. for 24 hours to obtain the activated strain; the plate medium is a nutrient agar medium;

[0041] Inoculate the activated strain of Bacillus jelly-like into the liquid medium in the shake flask, the inoculum amount is 5% of the mass of the medium, and place it in a shaker incubator with a rotation speed of 120 rpm and an air volume of 1 : 0.4, ...

Embodiment 2

[0074] Embodiment 2 A kind of production method of microbial bacterial agent

[0075] Step 1, strain selection

[0076] The bacterial classification that adopts is identical with embodiment 1.

[0077] Step 2. Strain activation and expansion

[0078] (1) Activation and expansion of jelly-like bacillus

[0079] Inoculate the jelly-like Bacillus strains on a plate medium, and cultivate them at 32°C for 24 hours to obtain activated strains; the plate medium is a nutrient agar medium;

[0080] Inoculate the activated strain of Bacillus jelly-like into the liquid medium in the shake flask, the inoculum amount is 5% of the mass of the medium, and place it in a shaker incubator with a rotation speed of 120 rpm and an air volume of 1 : 0.4, cultured at 35°C for 21 hours to obtain the expansion seed solution;

[0081] The formula of the liquid medium is: sweet potato powder 17g, soybean meal powder 14g, sodium alginate 0.7g, yeast extract 4.2g, glutamic acid 0.03g, ox bile salt 0.0...

Embodiment 3

[0111] Embodiment 3 A kind of production method of microbial bacterial agent

[0112] Step 1, strain selection

[0113] The bacterial classification that adopts is identical with embodiment 1.

[0114]Step 2. Strain activation and expansion

[0115] (1) Activation and expansion of jelly-like bacillus

[0116] Inoculate the jelly-like Bacillus strain on a plate medium, and culture it at 34°C for 24 hours to obtain the activated strain; the plate medium is a nutrient agar medium;

[0117] Inoculate the activated strain of Bacillus jelly-like into the liquid medium in the shake flask, the inoculum amount is 5% of the mass of the medium, and place it in a shaker incubator with a rotation speed of 120 rpm and an air volume of 1 : 0.4, cultivated at 36°C for 22 hours to obtain the expansion seed solution;

[0118] The formula of the liquid medium is: sweet potato powder 18g, soybean meal powder 15g, sodium alginate 0.8g, yeast extract 4.5g, glutamic acid 0.04g, ox bile salt 0.05...

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Abstract

The invention provides a microbial agent and a preparing method thereof. The microbial agent is prepared from jelly type bacillus, bacillus subtilis and bacillus licheniformis. In the microbial agent, the viable count of jelly type bacillus ranges from 1.8 billon cfu / g to 2.2 billon cfu / g, the viable count of bacillus subtilis ranges from 1.7 billon cfu / g to 1.9 billon cfu / g, and the viable count of bacillus licheniformis ranges from 1.2 billon cfu / g to 1.3 billon cfu / g. The preparing method of the microbial agent comprises the steps of strain selection, strain activation and expanding culture, strain fermentation and finished product obtaining. The microbial agent is high in strain content, low in infectious microbe rate and capable of effectively improving soil properties.

Description

technical field [0001] The invention provides a microbial bacterial agent and a production method thereof, belonging to the technical field of microbial fermentation. Background technique [0002] Microbial agents refer to a specific class of products containing living microorganisms. It uses the process and products of microbial life activities to improve the nutritional conditions of crops, exert the potential fertility of the soil, stimulate the growth and development of crops, and resist the harm of pathogens, thereby improving crop yield and quality. It does not directly provide nutrients to plants like ordinary fertilizers, but its role cannot be ignored. [0003] Microbial agents aim at the expansion of crop cultivation and seedling transfer area, and the increase of continuous cropping and heavy cropping, which further leads to increasingly serious soil problems, worse and worse crop output, and difficult to guarantee quality, which can well improve the above proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125C12R1/10C12R1/07
CPCC12N1/20
Inventor 王伟华陈凤霞
Owner SHANDONG HETIANWANG BIOLOGICAL TECH CO LTD
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