Application of bacillus siamensis B11 in prevention and/or treatment of bamboo shoot blight

A technology of bacillus, blight, applied in application, bacteria, fungicide, etc.

Inactive Publication Date: 2020-10-30
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports on the use of Bacillus sia

Method used

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  • Application of bacillus siamensis B11 in prevention and/or treatment of bamboo shoot blight
  • Application of bacillus siamensis B11 in prevention and/or treatment of bamboo shoot blight

Examples

Experimental program
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Effect test

Embodiment 1

[0015] Example 1 Isolation and identification of Bacillus siamese B11

[0016] 1. Isolation of Bacillus siamese B11

[0017] The Bacillus siamensis (Bacillus siamensis) B11 strain of the present invention was isolated on June 15, 2017 in the rhizosphere soil of the bamboo cultivation area in Yibin, Sichuan by the plate dilution coating method, specifically the soil samples taken back were diluted with sterile water , diluted 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 A total of 5 gradients, draw 100μL of 10 -3 , 10 -4 , 10 -5 These three gradient soil dilutions were coated on NA plates (3g of beef extract, 10g of peptone, 5g of sodium chloride, 15-20g of agar powder, 1000mL of distilled water, pH7.0, mixed and packed, and autoclaved at 121°C for 30min. ), each treatment was repeated three times, cultured at 27°C for 48 hours, and single colonies with obvious differences in colony morphology were picked to enter the primary screening.

[0018] According to the differences i...

Embodiment 2

[0032] The preparation of embodiment 2 bacillus siamese B11 nano water agent

[0033] The Bacillus siamese B11 is fermented, solidified, and dried to make a nano-sustained release agent, which specifically includes the following steps:

[0034] (1) Fermentation: Inoculate Bacillus siamese slant seeds in liquid medium with an initial inoculum size of 4%, then shake and culture at 25°C, 200r / min, and ventilation rate 100L / h for 46h;

[0035] Wherein, the liquid medium contains the following components by weight percentage: 0.1% nano silicon dioxide, 2% glucose, 0.11% soybean powder, 0.1% potassium chloride and 0.05% magnesium sulfate, and the rest are sterile water, liquid medium The pH value is 7.0.

[0036](2) Preparation of nano-aqueous agent: add humic acid and polyethylene glycol to the above fermentation liquid, mix well, then add gum arabic and cornstarch to solidify, then filter, freeze-dry at low temperature, and absorb the carrier attapulgite The nano sustained-relea...

Embodiment 3

[0038] The prevention and treatment experiment of embodiment 3 bamboo shoot dry blight

[0039] Preparation of Bacillus siamese B11 nano-sustained-release agent dilution: get 1g of Bacillus siamese B11 nano-sustained-release agent that embodiment 2 makes, carry out 50 times, 100 times, 200 times, 400 times, 800 times respectively with sterile water times, 1600 times and 2400 times to make dilutions, and its spore content is respectively 2 × 10 7 cfu / ml, 1×10 7 cfu / ml, 5×10 6 cfu / ml, 2.5×10 6 cfu / ml, 1.25×10 6 cfu / ml, 6.25×10 5 cfu / ml, 4.17×10 5 cfu / ml.

[0040] In bamboo shoot cultivation fields, through investigation and identification of pathogens, dry blight was caused by Anthostomella sp., Ascotremellopsis sp., and Cercosporidium sp., which were divided into four occurrence areas according to the degree of dry blight (I: 5-10%; II: 11% -30%; III: 31-60%; IV: >60%). In April-May, use the above-mentioned diluted solution to treat the bamboo shoots by spraying method,...

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Abstract

The invention discloses an application of bacillus siamensis B11 in prevention and/or treatment of bamboo shoot blight. The separated bacillus siamensis B11 strain has a special effect on the bamboo shoot blight, is high in environmental suitability and good in affinity, is suitable for commercial production, meets the strategic requirement of sustainable development, and has a wide market prospect.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to the application of Bacillus siamese B11 in the prevention and / or treatment of bamboo shoot blight. Background technique [0002] The dry blight of bamboo shoots caused by Anthostomella sp., Ascotremellopsis sp., and Cercosporidium sp. mainly harms young shoots, and when it is severe, semi-adult bamboos or young bamboos are also diseased, and bamboo shoots die in pieces. Bamboo shoot blight has been found in bamboo shoots of Pleioblastus amarus (Keng) keng, Phyllostachys heteroclada Oliver, Phyllostachys bissetii (McClure), Chimonobambusa quadrangularis (Fenzi) Makino and other varieties of bamboo shoots. To irregular lesions, the diseased part loses its normal fresh color, and gradually develops, and the young shoots turn brown, necrotic, and shrink, and finally dry up. The shells of diseased bamboo shoots are generally easy to peel off, and the stems of bamboo shoots ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/22C12N1/205C12R2001/07
Inventor 李姝江曾艳玲方馨玫王诗玮朱天辉韩珊谯天敏
Owner SICHUAN AGRI UNIV
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