The invention discloses bacillus velezensis and application thereof in vinegar brewing. The strain is bacillus velezensis QH-20003, which has been deposited in the China General Microbiological Culture Collection Center at Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, on January 13, 2021 with a deposit number of CGMCC No.21613. The bacillus velezensis is applied to cellar vinegar brewing, can grow, metabolize and produce enzymes under an acidic condition, has a capability of producing high-activity acid-resistant granular amylase, pullulanase and protease, can improve utilization rate of raw materials, provides a new enzyme source and a beneficial microbial fermentation strain for the vinegar brewing industry, thereby improving quality of a vinegar product and improving flavor of the vinegar.