175 results about "Bacillus ramosus" patented technology

The invention relates to bacillus velezensis and application thereof in preparation of a water-soluble microbial agent, and belongs to the technical field of microorganisms. The bacillus velezensis has the effects of producing exopolysaccharides, promoting the formation of soil aggregates, degrading abamectin, hydrolyzing protein, producing indoleacetic acid, promoting the growth of tomato plants and promoting the enzyme activity of tomato rhizosphere soil enzyme. The fertilizer not only has the effects of improving the soil aggregate structure and promoting the growth of tomato plants, but also can accelerate the temperature rise of the abamectin fermentation waste residue compost, prolong the high-temperature period and shorten the compost period, and is beneficial to promoting the harmless treatment and resource utilization of the abamectin fermentation waste residue.

The invention discloses bacillus velezensis and application of the bacillus velezensis in prevention and treatment of basal stem rot, the classification name of the bacillus velezensis is bacillus velezensis GN-22, the bacillus velezensis is preserved in China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus velezensis is CCTCC NO: M2025566, and the preservation date is March 24, 2025. The bacillus velezensis GN-22 disclosed by the invention belongs to rhizosphere growth-promoting bacteria, has a remarkable inhibition effect on different pathogenic fungi such as fusarium graminearum, fusarium pseudograminearum, fusarium asiaticum, fusarium verticillioides and fusarium oxysporum, and plays an important role in biological control of diseases such as corn and wheat stem rot; meanwhile, the strain has a growth promoting effect and can promote corn growth. The strain provided by the invention has the advantages of simple culture conditions, easiness in preservation, easiness in industrial production and good development and application prospects.

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention provides bacillus velezensis and application of the bacillus velezensis in co-production of 6-methyl-2-heptanone and 2, 4-di-tert-butylphenol, and belongs to the technical field of biological engineering. The bacillus velezensis is classified and named as bacillus velezensis LT-22, and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: M2023779, and the preservation date is May 19, 2023. According to the bacillus velezensis, the 6-methyl-2-heptanone and the 2, 4-di-tert-butylphenol can be co-produced, and the concentrations of the synthesized 6-methyl-2-heptanone and the 2, 4-di-tert-butylphenol can reach 75.72 mg / L and 42.48 mg / L; compared with chemical synthesis, the method for preparing the 6-methyl-2-heptanone and the 2, 4-di-tert-butylphenol is low in production cost, simple to operate, high in safety, environment-friendly and suitable for industrial popularization.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

A preparation method of a rice straw biochar loaded with Bacillus cereus, comprising: (1) washing, drying, grinding and sieving rice straws; (2) performing anaerobic pyrolysis treatment on the rice straws treated in the step (1) at 300-700° C. to obtain a biochar; (3) treating the biochar obtained in the step (2) with hydrochloric acid, then washing until a washing solution is neutral, drying, grinding, sieving and sterilizing to obtain a sterilized biochar; and (4) performing mixed culture on the sterilized biochar and a solution of Bacillus cereus subjected to activation culture, and then centrifuging after the end of culture to remove supernatant, so as to obtain a rice straw biochar loaded with Bacillus cereus having a carbon immobilizing capability.

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention discloses a bacillus capable of efficiently degrading fibers and application of the bacillus. The name of the bacillus altitudinis DF34 is bacillus altitudinis, the preservation number of the bacillus altitudinis is GDMCC No: 65492, and the bacillus altitudinis is preserved in Guangdong Microbial Culture Collection Center on December 4, 2024. The bacillus has the characteristics of strong stress resistance and high growth speed; the strain has the capability of efficiently degrading fibers, and can degrade fiber components of forage grass, promote fermentation and improve the fermentation quality of the forage grass with low soluble sugar water content in the ensiling process. Therefore, the bacillus altitudinis DF34 can be widely applied to various regions in China, and especially has an outstanding effect on silage raw materials with high fiber content. The strain successfully solves the problem of poor silage fermentation caused by low water-soluble carbohydrate content, and provides a new solution for improving the quality of the silage.

The invention discloses a drought-resistant bio-organic fertilizer and a preparation and application method thereof, and belongs to the technical field of bio-organic fertilizers. The water-retention living organic fertilizer comprises an organic component, a drought-resistant microorganism and an active synergist in a mass ratio of 70: 1: (0.5-1), the drought-resistant microorganisms comprise monascus xerophilous, bacillus megatherium and bacillus niamurensis in a volume ratio of 1: 1: 1. According to the whole process, through a multi-dimensional cooperation mechanism, the problems that a traditional water-retaining agent conflicts with the activity of microorganisms, and the function of a single strain is limited are solved, the soil activity is adjusted, the soil ecological activity is improved, and an efficient and low-cost solution is provided for arid region soil remediation and water-saving agriculture.

The invention relates to bacillus velezensis CGMCC (China General Microbiological Culture Collection Center) No.32593 as well as preparation and application of a microbial agent of the bacillus velezensis, and belongs to the technical field of microorganisms. The strain disclosed by the invention has the effects of dissolving phosphorus, dissolving potassium, fixing nitrogen and producing indoleacetic acid (IAA) at the same time. The method comprises the following steps: inoculating bacillus velezensis CGMCC No.32593 into an LB liquid culture medium, and fermenting by using a 5L fermentation tank to obtain the liquid microbial agent. The microbial agent provided by the invention is simple in preparation method and convenient for industrial production. By applying the liquid microbial agent, the plant height of tomatoes and the fresh weight of overground parts can be effectively increased, and the utilization rate of soil phosphorus in crop production is increased.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention discloses application of bacillus subtilis BY-S1 in preparation of biological nano-selenium, and belongs to the technical field of microbiology and biological nano-selenium preparation. The preservation number of the bacillus subtilis BY-S1 is CCTCC (China Center for Type Culture Collection) NO: M 20232368. Research finds that the bacillus subtilis BY-S1 has the function of converting and synthesizing the biological nano-selenium by utilizing selenite, the strain BY-S1 and the biological nano-selenium converted and synthesized by the strain BY-S1 can remarkably promote plant growth, and when the bacillus subtilis BY-S1 and the biological nano-selenium are combined for use, a synergistic interaction effect is achieved. A new microbial resource is provided for biosynthesis of nano-selenium, a stable, safe and efficient nano-selenium preparation method is provided, and a theoretical basis is laid for research of a novel plant growth promoter.

The invention relates to the technical field of pulping, in particular to a bamboo reed raw material biological fermentation cellulose pulping method and application thereof. Bacillus licheniformis, bacillus subtilis, bacillus pumilus and bacillus cereus are compounded into a complex microbial inoculant, the fermented bamboo reed raw material is used for pulping, all strains are mutualistic and synergistic, meanwhile, a rich complex enzyme system is provided, the prepared paper is good in physical performance and high in quality, pulping is conducted through biological treatment, and the paper is environmentally friendly and environmentally friendly. The use of a large amount of chemical agents in the traditional pulping is avoided, and the discharge of wastewater in the traditional pulping process is reduced.

The invention discloses a compound microbial agent formula for a corn seedling stage, and relates to the technical field of agricultural microorganisms. The microbial agent comprises bacillus subtilis and bacillus mucilaginosus, auxin hormone sodium naphthalene acetate, chelated zinc and a pH regulator potassium fulvate are added, and humic acid powder is used as a carrier. The dry powder preparation with the particle size being smaller than or equal to 200 meshes and the suspension rate being larger than or equal to 90% is prepared through a specific fermentation technology and a bacterial liquid mixed adsorption and spray drying technology. Field trials show that the composition can increase the emergence rate of corn from 85% to 90%-93%, increase the dry weight of the root system by 30%, and reduce the incidence rate of white seedling disease by 70% or above. Microbe, chemistry and agronomy principles are fused, and the corn production problem in the arid region is systematically solved; chemical fertilizer dependence is reduced, degraded soil is restored, and green agricultural policy guidance is met; the average cost per mu is less than 100 yuan, and the method is suitable for large-scale planting and precise irrigation systems.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention belongs to the technical field of microbial control, and particularly relates to a compound microbial agent as well as a preparation method and application thereof. The preparation method of the compound microbial agent comprises the following steps: (1) respectively activating bacillus megatherium, bacillus marinus N6-2 and lactobacillus plantarum A37 to obtain various strain activating solutions; mixing the activation solutions of the strains to prepare a mixed bacterial solution; (2) inoculating the mixed bacteria solution into a seed culture medium to prepare a seed solution; and (3) inoculating the seed solution into a fermentation culture medium for fermentation, and then drying and crushing to obtain the compound microbial agent. According to the prepared compound microbial agent, the total number of effective viable bacteria is increased by optimizing the composition of the culture medium. After the compound microbial agent is used, the contents of organic matters, quick-acting nitrogen, quick-acting phosphorus and quick-acting potassium in soil can be remarkably increased, and the soil fertility is improved, so that the growth of tomatoes is promoted, the yield and quality of the tomatoes are improved, and the compound microbial agent has a wide application prospect.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention discloses a compound microbial agent and application thereof to improvement of saline-alkali stress resistance of crops. The compound microbial agent is prepared from the following components in parts by weight: 1 to 3 parts of bacillus cereus, 1 to 3 parts of bacillus megatherium, 6 to 8 parts of rhizopus heterotropoides, 50 to 70 parts of gypsum powder, 10 to 30 parts of humic acid, 2 to 3 parts of yeast metabolite, 2 to 5 parts of aluminum sulfate, 2 to 5 parts of citric acid and 0.4 to 0.6 part of an auxiliary agent. The bacillus cereus, the bacillus megatherium and the rhizopus heterosporioides are combined for the first time, the biological functions of the bacillus cereus, the bacillus megatherium and the rhizopus heterosporioides are synergistically exerted, the growth performance of crops under saline-alkali stress is remarkably improved, the physicochemical properties and nutrient conditions of saline-alkali soil are effectively improved by adding humic acid, yeast metabolite and other components, the soil fertility is improved, and the saline-alkali soil quality is improved. Meanwhile, a favorable environment is provided for the growth of the microbial agent; the plant root activity is remarkably improved, the salt tolerance of crops under the salt and alkali stress condition is improved, the growth state of the plants is improved, and therefore the yield of the crops is increased.

The invention discloses bacillus velezensis with antagonism on cotton verticillium wilt and high salt stress resistance and application, the strain has the advantages of resisting saline and alkaline and antagonizing the cotton verticillium wilt at the same time, the problem of verticillium wilt in the existing saline-alkali soil cotton planting process is solved, the utilization of saline-alkali soil is expanded, and the cotton yield is increased.

The invention discloses application of bacillus albordowii in cultivation for improving the flavor of fragrant rice, and relates to the field of fragrant rice cultivation and planting. According to the application, it is found for the first time that bacillus albordowii has the effect of improving flavor substances of fragrant rice, and when fragrant rice seedlings grow to the three-leaf and one-core stage, the roots of the seedlings are soaked with bacillus albordowii liquid; after the seedlings are transplanted to a field, the bacillus albopastoris bacterial liquid is sprayed to the soil at the roots of the seedlings, 24 main aroma components in the fragrant rice can be increased by 5.56%-12036.00%, the content of 2-ethyl-1-hexanol with rose fragrance is increased by 120 times or above, the content of 2-butyl-1-octanol with flower fragrance is increased by 235.71%, the content of 6, 10-dimethyl-(E)-5, 6-dimethyl-2, 6, 6-tetramethyl-1, 3-pentanedione with fruit fragrance is increased by 235.71%, and the content of 6, 10-dimethyl-(E)-5, 6-tetramethyl-1, 3-pentanedione with fruit fragrance is increased by 235.71%. The content of 2, 9-undecylene-2-ketone is increased by 132.50%, the content of limonene, the content of hexanal, the content of 2-heptanone, the content of 2-tridecanone and the content of 2-pentadecanone are increased by 50% or above, and the content of 2-acetyl-1-pyrroline (2-AP) is increased by 30% or above; the application method is more convenient to operate, and the overall flavor of the rice is improved more remarkably.

The invention discloses bacillus velezensis FSUXF-717 capable of promoting diastatic fermentation and improving flavor of fermented wine and application of the bacillus velezensis FSUXF-717, and relates to the technical field of microorganisms. The preservation number of the bacillus velezensis FSUXF-717 provided by the invention is GDMCC No: 64133, the fermentation liquor and microbial inoculum of the strain can promote the saccharification process of the wine, and meanwhile, the strain can enhance the fermentation to significantly increase the total amino acid content (plt: 0.05) and increase the variety and content of flavor substances such as esters and organic acids, and has a promoting effect on the flavor formation of the wine body. The acquisition of the strain provides a strain resource for the development of wine brewing and fermented food, has important practical significance for promoting high-efficiency and high-quality wine brewing, and also provides a theoretical basis and technical support for the directional regulation and control of the quality of traditional fermented food.

The invention provides a pet hair decomposer with synergistic effect of bacillus subtilis and keratinase, the pet hair decomposer comprises bacillus subtilis ZYpet-015 and keratinase, the preservation number of the bacillus subtilis ZYpet-015 is CGMCC (China General Microbiological Culture Collection Center) No.34790, and the preservation date is June 6, 2025; the protease activity of the bacillus subtilis is greater than or equal to 129.92 U, the survival rate of the bacillus subtilis in simulated gastric juice within 3 hours is greater than 50%, and the tolerance rate of the bacillus subtilis in intestinal juice within 4 hours is greater than 90%. According to the pet hair decomposing agent, hair keratin is synergistically degraded through protease secreted by the bacillus subtilis ZYpet-015 and keratinase, and the hair decomposing efficiency reaches 28.07% + / -2.79% and is remarkably higher than that of a single treatment group. Meanwhile, experiments prove that the pet hair decomposer can effectively destroy the hair cuticle structure, is suitable for a pet hair-melting nutritional supplement and can relieve the hair ball symptom.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention provides an algal-bacterial symbiont constructed by bacillus subtilis and scenedesmus obliquus and application thereof, and the algal-bacterial symbiont provided by the invention can effectively absorb nutrient substances in soil, promote the improvement of soil fertility, improve soil structure and enhance the stability of soil microflora, so that the algal-bacterial symbiont has a good application prospect. When applied to soil for soybean planting, the fertilizer has the effects of promoting growth of crops such as soybeans, increasing crop yield and the like. The algal-bacterial symbiont microbial inoculum based on bacillus subtilis and scenedesmus obliquus is expected to be developed into an efficient and environment-friendly soil conditioner, and has a good application prospect.

The invention discloses a microorganism-nano synergistically reinforced cement-based composite material for self-repairing concrete cracks, which realizes the self-repairing and performance improvement of the cracks through the synergistic effect of bacillus pseudofirmus, carbon nanotubes, nano magnesium oxide and red mud. The material has the following characteristics: bacillus pseudofirmus is used as a microbial remediation agent, and dormant spores are activated when meeting water and oxygen and then are subjected to a series of biochemical reactions to induce calcium carbonate precipitation products so as to effectively fill microcracks; the carbon nano tube network structure not only enhances the mechanical property of the concrete, but also can provide a channel for directional deposition of a repairing substance; the nano magnesium oxide compensates shrinkage stress through hydration reaction, and meanwhile, a proper alkaline environment is maintained to guarantee microbial activity; the industrial waste red mud is used for replacing part of cement, so that carbon emission is reduced, and microorganisms are fixed by utilizing the porous characteristic of the red mud.

The bacillus is classified and named as bacillus velezensis SAU-MC-3X, the bacillus velezensis SAU-MC-3X is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.34098, and the preservation time is April 3, 2025. The invention also discloses a screening method of the bacillus, a strain fermentation characteristic and a protease production characteristic in application of rice wine koji. The bacillus velezensis obtained by the invention has huge application potential in rice wine koji.

The invention discloses a modifier for reducing wine grape soil obstacles and a preparation method and application thereof, and belongs to the technical field of soil improvement. According to the invention, the specific complex microbial inoculant composed of bacillus subtilis, bacillus licheniformis and trichoderma harzianum is adopted to ferment the grape pomace, antibacterial substances and organic acids can be generated, and saline-alkaline obstacles of soil can be relieved; the fermentation product contains a large amount of bacillus subtilis, bacillus licheniformis and trichoderma harzianum, and can occupy the ecological niche of the root system through competition, optimize the rhizosphere environment and improve the biological enzyme activity of soil. Through the synergistic effect with the decomposed straw, the humic acid and the furfural residues, the rhizosphere microenvironment can be remarkably improved, decomposition and nutrient conversion of organic substances in soil are accelerated, the soil fertility is improved, root systems can better extend and absorb nutrients, plant growth is promoted, and the yield and quality of fruits are improved.

The invention discloses a composite microcapsule of bacillus subtilis and trichoderma asperellum, which is prepared by the following steps: embedding bacillus subtilis Pn1 and trichoderma asperellum Ta1 by adopting sodium alginate, and carrying out microencapsulation treatment, so that the controllable release of the bacillus subtilis Pn1 and the trichoderma asperellum Ta1 is realized; therefore, the stability of the composite biocontrol bacterium under adverse environmental factors is improved, meanwhile, the inhibition effect on root rot bacteria is enhanced, and the sodium alginate embedded bacillus subtilis Pn1 and trichoderma asperellum Ta1 have important application value in prevention and treatment of panax notoginseng biological root rot.

The invention relates to the technical field of silage, in particular to a whole-plant corn silage additive and application thereof. The whole-plant corn silage additive is prepared from bacillus subtilis GW-910 and bacillus subtilis GW-908. The whole corn silage is carried out by adding the bacillus with lignocellulose degradation capability as the silage additive, so that the concentrations of lactic acid and acetic acid are remarkably increased, the concentration of butyric acid is reduced, the contents of NDF, ADF, ADL and starch are remarkably reduced, the content of WSC is remarkably increased, and the quality of the whole corn silage is remarkably improved.

The invention discloses bacillus velezensis for promoting tomato growth and preventing and controlling tomato fusarium wilt and application of the bacillus velezensis. The bacillus velezensis SD-3 and the bacillus subtilis Bs-6 are compounded and have a growth promoting effect on tomato plants and root systems. The plant height, stem diameter, plant fresh weight and plant dry weight are obviously improved, and the root length, root node number, root tip number and root surface area are increased. The photosynthetic efficiency and the light energy conversion rate of the plant are improved. The bacillus velezensis SD-3 and bacillus subtilis Bs-6 compound fungicide synergistically act to improve the control effect of the tomatoes on the fusarium wilt, and the control effect can reach 78.63%.