99 results about "Bacillus ramosus" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention provides a control method for apple alternaria leaf spot, and belongs to the technical field of crop disease control. The prevention and treatment method comprises the following steps: spraying a foliar biological pesticide fertilizer to apple trees; the foliar biological medical fertilizer comprises a bacillus fermentation product and a trichoderma fermentation product, the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208, and the bacillus is at least one of bacillus amyloliquefaciens TS-1203, bacillus subtilis B1 and bacillus subtilis BS-1208; the trichoderma is at least one of T6 and Ym. The combined action of bacillus and trichoderma is utilized, the morbidity of apple alternaria leaf spot can be effectively reduced, the growth of apple trees can be promoted, the photosynthetic capacity is improved, the combination of disease prevention and yield increase is realized, and the method is environment-friendly and suitable for green agricultural development.

The invention discloses bacillus velezensis BR-1 and application of the bacillus velezensis BR-1 in prevention and treatment of economic crop diseases. The preservation number of the bacillus velezensis BR-1 is CCTCC NO: M 20251379. The bacillus velezensis BR-1 disclosed by the invention is high in adaptability and wide in growth condition range, and is more beneficial to application in agricultural production. Meanwhile, the bacillus velezensis BR-1 has a remarkable inhibition effect on the growth of various phytopathogens such as verticillium dahliae, fusarium oxysporum, sclerotinia sclerotiorum and ralstonia solanacearum, can remarkably reduce the morbidity of cotton verticillium wilt, fusarium wilt and tomato bacterial wilt under a greenhouse condition, and has a wide agricultural application prospect.

The invention belongs to the field of microorganisms, and relates to bacillus velezensis A111 and application thereof. The bacillus velezensis A111 disclosed by the invention can resist saline and alkaline, and has a relatively good inhibition effect on phytophthora capsici and ralstonia solanacearum. The bacillus velezensis A111 also has relatively strong capability of producing cellulase, amylase, protease, ACC deaminase and indole-3-acetic acid (IAA), and has a relatively good growth promoting effect on tomatoes, and a fungicide prepared from the biocontrol bacterium does not cause pollution to the ecological environment in the use process, and is pollution-free. By using the biocontrol bacterium disclosed by the invention, the dosage of other chemical pesticides can be reduced, the prevention and control cost of tomato diseases is reduced, the tomato growth is promoted, and the biocontrol bacterium has a good popularization value.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention discloses bacillus velezensis FSUXF-717 capable of promoting diastatic fermentation and improving flavor of fermented wine and application of the bacillus velezensis FSUXF-717, and relates to the technical field of microorganisms. The preservation number of the bacillus velezensis FSUXF-717 provided by the invention is GDMCC No: 64133, the fermentation liquor and microbial inoculum of the strain can promote the saccharification process of the wine, and meanwhile, the strain can enhance the fermentation to significantly increase the total amino acid content (plt: 0.05) and increase the variety and content of flavor substances such as esters and organic acids, and has a promoting effect on the flavor formation of the wine body. The acquisition of the strain provides a strain resource for the development of wine brewing and fermented food, has important practical significance for promoting high-efficiency and high-quality wine brewing, and also provides a theoretical basis and technical support for the directional regulation and control of the quality of traditional fermented food.

The invention provides a pet hair decomposer with synergistic effect of bacillus subtilis and keratinase, the pet hair decomposer comprises bacillus subtilis ZYpet-015 and keratinase, the preservation number of the bacillus subtilis ZYpet-015 is CGMCC (China General Microbiological Culture Collection Center) No.34790, and the preservation date is June 6, 2025; the protease activity of the bacillus subtilis is greater than or equal to 129.92 U, the survival rate of the bacillus subtilis in simulated gastric juice within 3 hours is greater than 50%, and the tolerance rate of the bacillus subtilis in intestinal juice within 4 hours is greater than 90%. According to the pet hair decomposing agent, hair keratin is synergistically degraded through protease secreted by the bacillus subtilis ZYpet-015 and keratinase, and the hair decomposing efficiency reaches 28.07% + / -2.79% and is remarkably higher than that of a single treatment group. Meanwhile, experiments prove that the pet hair decomposer can effectively destroy the hair cuticle structure, is suitable for a pet hair-melting nutritional supplement and can relieve the hair ball symptom.

The invention discloses recombinant bacillus subtilis with high yield of surfactin as well as a construction method and application of the recombinant bacillus subtilis, and belongs to the technical field of genetic engineering. According to the invention, non-essential genes are determined through analysis and identification, a simplified engineering strain is obtained, and the highest fermentation yield reaches 11.2 g / L; then, a PTS part sugar phosphorylase gene obtained through screening is transferred into the simplified engineering strain, the metabolism efficiency of carbohydrates is improved, transportation and utilization of the carbohydrates are optimized, a fatty acid transmembrane transporter gene is further transferred, fatty acid uptake is assisted, synthesis of surfactin is facilitated, and the yield of the surfactin is improved. The combination of the two realizes optimization of synthesis of surfactin through a composite carbon source. Compared with a simplified engineering strain, the genetically engineered bacterium obtained by the invention has the advantages that the yield of surfactin is increased by 57.1%, the yield is increased by 2.3 times compared with that of an unsimplified engineering strain EX, and the final shake-flask fermentation yield can reach 17.6 g / L. As an industrial chassis cell of high-yield lipopeptide, the cell has a good industrial application prospect.

The invention discloses a microorganism-nano synergistically reinforced cement-based composite material for self-repairing concrete cracks, which realizes the self-repairing and performance improvement of the cracks through the synergistic effect of bacillus pseudofirmus, carbon nanotubes, nano magnesium oxide and red mud. The material has the following characteristics: bacillus pseudofirmus is used as a microbial remediation agent, and dormant spores are activated when meeting water and oxygen and then are subjected to a series of biochemical reactions to induce calcium carbonate precipitation products so as to effectively fill microcracks; the carbon nano tube network structure not only enhances the mechanical property of the concrete, but also can provide a channel for directional deposition of a repairing substance; the nano magnesium oxide compensates shrinkage stress through hydration reaction, and meanwhile, a proper alkaline environment is maintained to guarantee microbial activity; the industrial waste red mud is used for replacing part of cement, so that carbon emission is reduced, and microorganisms are fixed by utilizing the porous characteristic of the red mud.

The invention discloses a modifier for reducing wine grape soil obstacles and a preparation method and application thereof, and belongs to the technical field of soil improvement. According to the invention, the specific complex microbial inoculant composed of bacillus subtilis, bacillus licheniformis and trichoderma harzianum is adopted to ferment the grape pomace, antibacterial substances and organic acids can be generated, and saline-alkaline obstacles of soil can be relieved; the fermentation product contains a large amount of bacillus subtilis, bacillus licheniformis and trichoderma harzianum, and can occupy the ecological niche of the root system through competition, optimize the rhizosphere environment and improve the biological enzyme activity of soil. Through the synergistic effect with the decomposed straw, the humic acid and the furfural residues, the rhizosphere microenvironment can be remarkably improved, decomposition and nutrient conversion of organic substances in soil are accelerated, the soil fertility is improved, root systems can better extend and absorb nutrients, plant growth is promoted, and the yield and quality of fruits are improved.

The invention discloses a composite microcapsule of bacillus subtilis and trichoderma asperellum, which is prepared by the following steps: embedding bacillus subtilis Pn1 and trichoderma asperellum Ta1 by adopting sodium alginate, and carrying out microencapsulation treatment, so that the controllable release of the bacillus subtilis Pn1 and the trichoderma asperellum Ta1 is realized; therefore, the stability of the composite biocontrol bacterium under adverse environmental factors is improved, meanwhile, the inhibition effect on root rot bacteria is enhanced, and the sodium alginate embedded bacillus subtilis Pn1 and trichoderma asperellum Ta1 have important application value in prevention and treatment of panax notoginseng biological root rot.

The invention relates to the technical field of silage, in particular to a whole-plant corn silage additive and application thereof. The whole-plant corn silage additive is prepared from bacillus subtilis GW-910 and bacillus subtilis GW-908. The whole corn silage is carried out by adding the bacillus with lignocellulose degradation capability as the silage additive, so that the concentrations of lactic acid and acetic acid are remarkably increased, the concentration of butyric acid is reduced, the contents of NDF, ADF, ADL and starch are remarkably reduced, the content of WSC is remarkably increased, and the quality of the whole corn silage is remarkably improved.

The invention discloses bacillus subtilis 12-10-2 and application thereof in synergistic preparation of fish oil and fish sauce, and belongs to the technical field of microbial fermentation. The preservation number of the bacillus subtilis (Bacillus subtilis) 12-10-2 is CGMCC (China General Microbiological Culture Collection Center) No.34966. The bacillus subtilis (Bacillus subtilis) 12-10-2 can be used for preparing the bacillus subtilis. The bacillus subtilis 12-10-2 can be used for efficiently hydrolyzing protein in a high-salt environment. The bacillus subtilis 12-10-2 is used for performing bacterium-adding fermentation on the snakehead processing leftovers, so that the fish oil and the fish sauce can be synergistically prepared, and the obtained product is high in yield and good in quality. A new microbial resource is provided for high-value utilization of snakehead processing leftovers, the problems of environmental pollution and resource waste caused by improper treatment can be solved, a new technical support is provided for establishment of a preparation process of high-quality fish oil and fish sauce, and the method has a wide market application prospect.

The application belongs to the technical field of microorganisms, and particularly relates to a halophilic bacillus, a bacterial agent and application thereof. The application provides a halophilic bacillus Halobacillus andaensis, and the preservation number is CCTCC M 20232394. The application also provides a bacterial agent containing the halophilic bacillus. The application also provides application of the halophilic bacillus or the bacterial agent in promoting plant growth under saline-alkali stress, improving plant stress resistance, improving soil, and crop production. The halophilic bacillus provided by the application has excellent saline-alkali tolerance, and can only grow under saline-alkali conditions. Meanwhile, the halophilic bacillus has growth-promoting characteristics such as salt reduction, acid production for alkali reduction, nitrogen fixation, and the like, can reduce salt damage, and improve crop yield and quality.

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.

The invention belongs to the technical field of biology, relates to a zearalenone degrading enzyme, and particularly relates to a bacillus subtilis source zearalenone degrading enzyme, an engineering strain thereof and an enzyme preparation prepared from the bacillus subtilis source zearalenone degrading enzyme. Compared with the known zearalenone degrading enzyme, the zearalenone degrading enzyme not only has obvious structure and sequence differences, but also shows an excellent degrading effect at low concentration. The lactone hydrolase derived from bacillus is found for the first time, and the biological resource basis of zearalenone degrading enzyme is expanded. The enzyme not only has significant difference from other degrading enzymes in structure, but also shows unique characteristics in degradation mechanism. The capability of efficiently degrading zearalenone shows that the bacillus subtilis has a wide application prospect in the fields of agriculture, food industry and the like. In addition, the invention also covers the structure of a degradation product generated by the zearalenone enzyme, and provides a new perspective for understanding a zearalenone degradation mechanism.

The invention discloses a preparation method of an ingredient containing waste milk and the ingredient. The ingredient is prepared from the following raw materials in parts by weight: 900-1100 parts of waste milk, 8-12 parts of a compound microbial preparation and 25-45 parts of a culture medium auxiliary material, the compound microbial preparation is prepared from bacillus subtilis, bacillus licheniformis, lysozyme and protease, wherein the mass ratio of the bacillus subtilis is 32 to 38 percent, the mass ratio of the bacillus licheniformis is 32 to 38 percent, the mass ratio of the lysozyme is 12 to 18 percent, and the mass ratio of the protease is 12 to 18 percent; the culture medium auxiliary material consists of protein powder, brown sugar powder and amino acid, and the mass ratio of the protein powder to the brown sugar powder to the amino acid is 1: 20: 5. According to the invention, the waste milk is used as a main raw material, so that the resource utilization of wastes is realized, the environmental pollution is reduced, and the production cost of the feed is reduced.

The application provides a salt-tolerant growth-promoting and disease-preventing bacterium B32 and application thereof in cucumber cultivation, and belongs to the technical field of agricultural microorganisms. Bacillus subtilis The salt-tolerant growth-promoting and disease-preventing bacterium B32 is named Bacillus subtilis (B32), is preserved in the China Center for Type Culture Collection, is preserved on January 4, 2023, has a preservation number of CCTCC NO: M2023019, has high salt tolerance and good growth-promoting characteristics, and has inhibiting effects on various crop diseases; the strain can significantly improve the salt-tolerance and growth-promoting capacity of cucumber under salt stress, can be effectively used for cucumber planting in saline-alkali land, and improves the yield and quality of cucumber.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

The invention relates to a preparation for preventing and / or treating sugarcane leukosis and application thereof, and relates to the technical field of biological agents. The preparation comprises bacillus mucilaginosus, bacillus megatherium, bacillus laterosporus, alternaria tenuissima activator protein, calcium acetate, magnesium sulfate and glucose. The preparation has a relatively good prevention and / or treatment effect on the sugarcane bacterial leaf streak, and also can promote the growth of the sugarcane at the same time.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

The invention belongs to the technical field of plant cultivation, provides application of bacillus H8 in cultivation of emilia sonchifolia, and particularly relates to bacillus H8, a bacillus H8 suspension with the concentration of 102-104 CFU / mL is prepared and applied to the position 2-3 cm away from the root of emilia sonchifolia as a bio-fertilizer once every 7 days, plant growth can be remarkably promoted, and the yield of emilia sonchifolia is increased. The content of medicinal components of chlorogenic acid and quercitrin in the leaves is increased; the method solves the problems of soil degradation, unstable medicinal material quality and the like caused by dependence on chemical fertilizers and pesticides in traditional cultivation, is suitable for green and efficient cultivation of emilia sonchifolia, and has the advantages of simplicity and convenience in operation, environment friendliness, low cost and the like.

The invention relates to the technical field of microorganisms, in particular to bacillus and application thereof in production of beta-glucosidase. The invention relates to bacillus LP-1, the Latin literature name of the bacillus LP-1 is bacillus sp.LP-1, and the preservation number of the bacillus LP-1 is CCTCC (China Center For Type Culture Collection) M 20232261. The invention further provides application of the bacillus LP-1 in the aspect of producing beta-glucosidase. The bacillus LP-1 has the advantages that the bacillus LP-1 has good capacity of producing the beta-glucosidase, the heat stability of the produced beta-glucosidase is relatively high, performance results show that the enzyme activity is 75.33 U / mL, the optimum reaction temperature is 80 DEG C, the optimum pH is 7.0, and the bacillus LP-1 has a great application prospect. The method has important significance for reasonably developing microbial resources in the Liupao tea and promoting the industrial development of the Liupao tea.

The invention provides composite expansive soil containing microorganisms-sisal fibers. The composite expansive soil comprises the sisal fibers, expansive soil, a bacillus pasteurii solution and a cementing solution. Wherein the mass part ratio of the bacillus pasteurii liquid to the cementing liquid is 3: (2-6); the mass part of the sisal fiber is 0.4-1.0; the concentration of the cementing liquid is 1.0 to 3.0 mol / L; the cementing liquid comprises the following components in parts by mass: 1 part of urea and 1-6 parts of calcium chloride. The composite expansive soil provided by the invention is improved by adopting a means of combining a microbe induced carbonate precipitation technology and fiber reinforcement, and the advantage complementation of microbe mineralization and physical reinforcement can be realized. The invention further provides a preparation method of the composite expansive soil containing the microorganisms-sisal fibers.

The invention relates to a complexing agent for promoting growth of medicinal plants as well as a preparation method and application of the complexing agent. Specifically, the complexing agent for promoting the growth of the medicinal plants is prepared from an effective amount of bacillus velezensis microbial inoculum, modified ammonium carbamate, phenylalanine, an agonist, a dispersing agent and a specific factor. The trinity synergistic effect of microorganisms, nutrition and signals is realized. The microbial preparation not only can inherit the advantage of environmental friendliness of the microbial preparation, but also can overcome the core defects of poor environmental adaptability, short persistence, unstable effect, single function and the like in the prior art. Especially for special crops with high quality requirements, such as medicinal plants, growth promotion, quality improvement and adversity resistance are realized, and the effect is stable and lasting.

The invention discloses a long-chain inulin steamed bun containing probiotics and a preparation method thereof, and relates to the technical field of food processing, the preparation method of the steamed bun comprises the following steps: culturing bacillus velezensis, adjusting the OD value of a bacterial liquid to 0.9, and adding the prepared bacillus velezensis bacterial liquid into mixed powder containing plain flour and long-chain inulin; then performing yeast activation, adding water and performing dough kneading to form dough; and then performing primary fermentation, shaping, secondary fermentation and steaming on the dough to obtain the long-chain inulin steamed bun. The optimal scheme that 0.5% of the addition amount of bacillus velezensis is determined according to parameters such as texture, form, moisture distribution and viable bacteria retention is adopted, the steamed buns prepared through the method are moderate in hardness and elasticity and reasonable in specific volume and porosity, viable bacteria are still retained after steaming, the adverse effect of long-chain inulin on the quality of the steamed buns can be improved, probiotics can be supplemented, and the steamed buns are healthy and healthy. Dietary choices of people are enriched, and industrial popularization is easy.

The invention provides bacillus zhangzhouensis and application thereof, and belongs to the technical field of biological agriculture, the bacillus zhangzhouensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.34248, the preservation number is CGMCC No.34248, and the preservation number is CGMCC No.34248. The bacterial strain and the biological agent containing the bacterial strain have a good effect of improving plant stress resistance (water stress resistance), can maintain basic physiological activities of crop plants under the condition of soil water shortage or waterlogging, can further promote growth of the crop plants, and achieve yield improvement and crop quality improvement. And the bacillus zhangzhou is collected from the natural environment, is safe, environment-friendly and pollution-free, and has a wide application prospect.

The invention provides cold-resistant bacillus subtilis for pond culture under a low-temperature condition. The preservation number of the cold-resistant bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) No.36068. The cold-resistant bacillus subtilis strain provided by the invention has remarkable comprehensive application advantages, can keep higher growth activity and metabolic capability in a low-temperature environment, and continuously plays a water body purification function. Ammonia nitrogen and nitrite nitrogen in a water body can be efficiently degraded, the environment of the aquatic water body is remarkably improved by promoting the nitrogen circulation process, and harm caused by nitrogen pollution is reduced. The strain is high in safety, does not cause poison or stress reaction to bred animals even under the condition of high-concentration use, and has good ecological safety and environmental friendliness.

This invention belongs to the field of peanut cultivation and nitrogen use efficiency regulation technology. This invention provides a method for improving the nitrogen use efficiency of peanuts, comprising the following steps: applying a mixture of Bacillus preparation and water, along with a polyglutamic acid solution, to the rhizosphere via root irrigation during the initial flowering stage, and spraying a first composition onto the peanut leaves; spraying a second composition onto the peanut leaves during the full flowering stage; and spraying a third composition onto the peanut leaves during the pod-filling stage; and specifying the composition of the first, second, and third compositions. This invention improves the nitrogen use efficiency of peanuts by regulating the peanut rhizosphere environment and foliar nutrient supply in stages, promoting rhizosphere microbial activity, increasing the level of endogenous hormones in the roots, and enhancing the plant's ability to absorb, translocate, and distribute nitrogen. This invention has a clear regulatory approach, a complete technical route, and is applicable to the production of different peanut varieties.