15 results about "Bacillus ramosus" patented technology

The present application relates to a kind of bacteria inhibiting and growth promoting microorganism and its compound microbial inoculant, preparation method and application.The present application provides a kind of bacillus subtilis (Bacillus subtilis) YCBA0319, preservation number is CGMCC No.33006;A kind of trichoderma longibrachiatum (Trichoderma longibrachiatum) YCFA0522, preservation number is CGMCC No.41655.In the technical solution of the present application, through bacillus subtilis and trichoderma longibrachiatum and the compound microbial inoculant of its composition, through the synergistic effect of two strains, it has the promotion effect to soil health and apple yield, quality etc., compared with control, soil bulk density is reduced, organic matter and available nutrient are improved, and apple single fruit size, yield and quality are all improved.

This application discloses a Bacillus subtilis strain JTFB-01 and its applications, belonging to the field of microbiology. The Bacillus subtilis strain JTFB-01 has the accession number CGMCC No. 37199 and is deposited at the China General Microbiological Culture Collection Center on December 25, 2025. Field trials show that this inoculant, applied through seed treatment, achieves control effects of 68.20%, 71.73%, and 72.15% against wheat take-all, sheath blight, and root rot, respectively, and significantly increases wheat plant height and yield. It also shows a field control effect of over 50% against cotton Verticillium wilt, with a yield increase of over 17%. This inoculant effectively controls soil-borne diseases and overcomes crop replanting obstacles through multiple mechanisms, including inhibiting pathogens, colonizing the rhizosphere, and promoting growth, thus possessing significant agricultural application value.

The present application relates to the technical field of microorganism, and particularly relates to a microbial composition, a compound microbial agent and application.The microbial composition of the present application comprises Bacillus amyloliquefaciens, Bacillus brevis and Trichoderma harzianum, the microbial composition of the present application can simultaneously prevent and treat rice seed production process rice blast and storage process aspergillus flavus pollution, realize the whole life cycle protection of rice seed from production to storage, solve the problem that different agents need to be used to prevent and treat rice blast and aspergillus flavus pollution in the prior art.In addition, the compound microbial agent containing the microbial composition is safe, efficient, environmentally friendly, can replace chemical pesticides, reduce environmental pollution and pesticide residues, and the preparation method is simple, easy to industrial production, and has wide application prospect.

This invention belongs to the field of peanut cultivation and nitrogen use efficiency regulation technology. This invention provides a method for improving the nitrogen use efficiency of peanuts, comprising the following steps: applying a mixture of Bacillus preparation and water, along with a polyglutamic acid solution, to the rhizosphere via root irrigation during the initial flowering stage, and spraying a first composition onto the peanut leaves; spraying a second composition onto the peanut leaves during the full flowering stage; and spraying a third composition onto the peanut leaves during the pod-filling stage; and specifying the composition of the first, second, and third compositions. This invention improves the nitrogen use efficiency of peanuts by regulating the peanut rhizosphere environment and foliar nutrient supply in stages, promoting rhizosphere microbial activity, increasing the level of endogenous hormones in the roots, and enhancing the plant's ability to absorb, translocate, and distribute nitrogen. This invention has a clear regulatory approach, a complete technical route, and is applicable to the production of different peanut varieties.

The application provides a high-salt-tolerant Bacillus subtilis capable of producing protease and nattokinase and an application thereof, the Bacillus subtilis is named as Bacillus subtilis 3-1, is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20252875, and the 16S rRNA nucleotide sequence is shown as SEQ ID NO: 1. The Bacillus subtilis of the application can be directly applied to a high-salt fermentation system due to excellent high-salt-tolerant performance, and solves the industrial problem that the activity of a conventional strain is inhibited; due to the protease production property, can be used for efficiently degrading raw material protein in the field of food fermentation, promoting flavor formation and shortening the production cycle; the nattokinase production property makes it have outstanding value in the field of functional food and preparation, and can be used for developing a fermented product with health care functions of dissolving thrombus, and improving the health added value of the product. The strain provides a core microbial resource for quality improvement and functional development of high-salt fermented food.

The present invention belongs to the field of biotechnology. Provided are recombinant Bacillus subtilis BLCY-013 and the use thereof in the preparation of mannose by using glucose as a raw material. The recombinant Bacillus subtilis BLCY-013 is deposited in the China General Microbiological Culture Collection Center (CGMCC) located at No. 3, Institute 1, Beichen West Road, Chaoyang district, Beijing, with the strain deposit number of CGMCC No. 32760 on 25 November 2024. Using glucose as a raw material, mannose is prepared by means of a bio-enzymatic reaction of the recombinant Bacillus subtilis, without the use of chemical reagents. The reaction exhibits a high specificity, and a significantly reduced raw material cost compared with conventional methods using fructose as a substrate. Unreacted glucose in the preparation process can be recycled, resulting in a high utilization efficiency of raw materials. By using evaporative crystallization and controlling crystallization parameters, the prepared mannose is anhydrous, exhibits a good flowability and a low hygroscopicity, and is less prone to agglomeration.

This invention provides a composition for controlling anthracnose in peppers and its preparation method, belonging to the field of plant disease control technology. The composition of this invention, by weight, comprises the following components: 30-55 parts of Bacillus fermentation culture broth, 5-17 parts of loquat leaf extract, 3-8 parts of hawthorn leaf extract, 2-6 parts of milk thistle extract, 1-5 parts of sodium aluminosilicate, 4-7 parts of polyethylene glycol, and 5-8 parts of ε-polylysine. This invention, by mixing Bacillus fermentation culture broth, compound plant extracts, sodium aluminosilicate, polyethylene glycol, and ε-polylysine, can effectively control anthracnose in peppers. The addition of sodium aluminosilicate, polyethylene glycol, and ε-polylysine to the formula prevents flocculation and precipitation, and improves the storage stability of the composition.

The application belongs to the technical field of Chinese medicine plant disease biological control, and particularly relates to a bacillus velezensis and application thereof. Bacillus velezensis The nsu-59 strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2025573. The bacillus velezensis provided by the application can not only prevent and treat the anthracnose of trichosanthes fruit, but also promote the seed germination of trichosanthes, and has a broad-spectrum antifungal effect on various Chinese medicine plant pathogenic fungi such as anthracnose, fusarium, trichoderma and diaporthe, has low prevention and treatment cost, is green and environment-friendly, will not cause drug resistance, and has great potential to be developed into a Chinese medicine plant biocontrol agent.

This invention discloses a Bacillus subtilis ( Bacillus subtle The EB-8 strain, deposited on January 14, 2026, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37320, is disclosed. The application of Bacillus subtilis strain EB-8 in the fermentation modification of tartary buckwheat flour is also disclosed. This invention's Bacillus subtilis strain EB-8 can significantly increase the amylose content of tartary buckwheat, helping to inhibit starch retrogradation and improve its freeze-thaw stability; simultaneously, it increases the total flavonoid and total phenolic content of tartary buckwheat, enhancing its antioxidant activity. Furthermore, this strain can selectively retain and enrich essential amino acids such as lysine and methionine, reduce bitter components, improve taste, and facilitate the formation of a cereal matrix suitable for cold processing, with a mild taste and enhanced oxidative defense. Overall, it achieves synergistic optimization of the functional activity and processing characteristics of tartary buckwheat flour, which is beneficial for the high-value utilization of tartary buckwheat flour resources.

This application discloses an emulsion composition, an emulsion, its preparation method, uses, and cosmetics, belonging to the field of chemical production technology. The technical solution is as follows: an emulsion composition containing at least two of Bacillus ferment, glycolipid emulsifiers, and phospholipid emulsifiers, and containing at least Bacillus ferment. This emulsion composition uses Bacillus ferment as the main emulsifier, combined with other biological emulsifiers, to enhance the application range and stability of the D-phase emulsion system. Furthermore, this application has also found through testing that when the D-phase emulsion system is used in conjunction with a special oil phase, the brightening and soothing abilities of the emulsion can be further enhanced.

This invention discloses a Bacillus subtilis strain and its applications, belonging to the field of microbial technology. A Bacillus subtilis strain (…) is provided. Bacillus subtilis DW-14, with accession number CGMCC No. 37076, deposited on December 15, 2025, by the China General Microbiological Culture Collection Center (CGMCC). Bacillus subtilis DW-14 possesses high cellulase and protease secretion capabilities. When applied to the fermentation process of microbial organic fertilizer, it can improve fermentation efficiency and the quality of the organic fertilizer. The resulting organic fertilizer, when applied to crop cultivation, can improve soil, suppress crop diseases, and increase yield and quality.

This invention discloses a strain of *Bacillus pallida* X41, its inoculant, and its applications, relating to the field of agricultural microbiology. This invention is the first to isolate a strain of *Bacillus pallida* X41 from the boundary between apple rotten lesions and healthy fruit. Aeribacillus pallidus ​ X41, a strain possessing the ability to produce siderophores, hydrolyze inorganic phosphorus, and produce auxin, can inhibit the growth of the pathogen Botrytis cinerea. Experiments have shown that the fermentation broth of this strain not only significantly reduces the size of apple rot lesions, increases antioxidant enzyme activity, and maintains apple fruit quality, but also promotes sugar conversion and increases the content of flavonoids in the fruit, thereby enhancing fruit resistance. This fermentation broth can be used to prepare a preservative to reduce post-harvest rot in apples; the process is convenient, safe, and environmentally friendly, and has broad application prospects in apple storage and preservation.

The application discloses a bacillus velezensis strain and application thereof in degrading COD of wastewater, and belongs to the technical field of biological degradation. The bacillus velezensis strain can adapt to the environment of PTA wastewater and agricultural breeding wastewater, and grows and reproduces well, and has a good degradation and removal effect on the COD component in the wastewater, the degradation rate of the COD in the PTA wastewater can reach 50.50%, and the degradation rate of the COD in the agricultural breeding wastewater can reach 96.55%. The strain can effectively degrade the COD in the wastewater, and is beneficial to sustainable development of an ecological environment.

The application discloses bacillus safensis with weed seed germination inhibition function, compost prepared therefrom and application. The application screens a strain NG-50 which can efficiently degrade cellulose, has excellent nitrogen fixation function and good stress resistance function, can resist drought stress, saline-alkali stress, acid stress and high temperature stress by isolating microorganisms in soil after straw field return in desert edge. The strain NG-50 is identified as bacillus safensis by molecular biology, and its microorganism preservation number is CGMCC No. 35363. It is proved by tests that the strain NG-50 can effectively inhibit weed seed germination and significantly promote crop growth. Application of the strain NG-50 in preparation of agricultural and forestry waste compost can significantly shorten the fermentation period of the compost and obviously improve the compost quality, and the strain NG-50 has potential as a composting agent for agricultural and forestry waste.

This application discloses a strain of Bacillus belyceae DW4-5-2 for controlling pear fire blight and develops a highly efficient and stable microbial preparation fermentation method for it, clarifying its application in the control of pear fire blight. Compared with existing technologies, this application has the following advantages: the strain is highly novel; it has high antagonistic activity; it has excellent pot-plant control efficacy and a long-lasting effect; the fermentation process is thoroughly optimized; and it has broad application prospects. This strain and its optimized fermentation process provide core technical support for the development of highly efficient, stable, and environmentally friendly biological pesticides for pear fire blight, which is of great significance for ensuring the green, safe, and sustainable development of my country's pear and apple industries.