Long chain recombinant human bone morphogenesis protein-2 and its preparation method and uses

Abstract

The invention discloses a long-chain recombination human bone pattern generating protein-2 and preparing method and application, which is characterized by the following: utilizing gene project technique to grow the protein in the expressing system of escherichia coli and bacillus subtilis; adopting human bone sarcoma cell mRNA to do reverse transcription to obtain the cDNA as form; augmenting nucleotide sequence of entire 114 amino acids naturally from carboxyl end; adding a segment of nucleotide in front of the first codon of primer 5' end; increasing a segment of polypeptide at N end corresponding to amino acid sequence; obtaining long-chain rhBMP-2 gene with molecular weight at 30KD and purity over 95%.

Description

technical field

[0001] The invention relates to a recombinant human bone morphogenetic protein-2 and its genetic engineering preparation method. Background technique

[0002] Delayed fracture union, bone nonunion, and repair of bone defect have always been major unresolved problems in the field of orthopedics. For a long time, the repair of bone defects has mainly adopted three treatment methods: autologous bone graft, allogeneic bone graft, and replacement and repair with biomaterials and products. Autologous bone is limited by the number of sources, and there are at least 10% surgical complications in bone harvesting operations, and a long crawling replacement process is required after implantation; allogeneic bone has different degrees of immune rejection and potential risk of disease transmission. Therefore, biomaterials and products with special functions have attracted much attention clinically. Although many biomaterials have been used clinically, the lack of activi...

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