The invention discloses a preparation method and application of athlete-derived
bifidobacterium probiotics for intestinal barrier repair, and relates to the technical field of
biochemistry and microorganisms, and the preparation method comprises the steps of special
bacteria source sampling, directional separation and purification, molecular biological identification,
antibiotic sensitivity evaluation and
injury repair verification. According to the preparation method and application of the sportsman-derived
bifidobacterium probiotics for repairing the intestinal barrier, the sportsman intestinal
flora is used as an exclusive bacterial source, and the unique metabolic elite attribute and stress adaptability of the sportsman intestinal
flora are utilized to directionally screen and obtain a
bifidobacterium strain adaptive to an extreme
physiological stress environment; the
bottleneck that conventional probiotics are only suitable for a common physiological environment and the
survival rate and activity in a stress state are greatly reduced is broken through,
high survival rate and
metabolic activity can be kept in extreme environments such as exercise
heat stress and the like, and accurate repair of intestinal barriers is realized through multiple mechanisms; meanwhile, an exclusive screening, identification and safety
evaluation system is established, so that the safety and functional specificity of the strain are ensured.