Bacillus velezensis and application thereof in wheat sheath blight control and growth promotion

A technology of wheat sheath blight and Bacillus, applied in the direction of application, microbial-based methods, chemicals for biological control, etc., can solve the problem that does not involve Bacillus Velez, does not report siderophilic biopromoting substances, etc. problem, to achieve the effect of promoting effective utilization, good inhibition effect and high safety

Active Publication Date: 2019-04-02
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many studies on Bacillus Velez, mainly focusing on the application of this strain for biological control, but there is no report that this strain can produce indole acetic acid (IAA), siderophilic biomass, or Not involved Bacillus Velez has the effect of dissolving organophosphate

Method used

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  • Bacillus velezensis and application thereof in wheat sheath blight control and growth promotion
  • Bacillus velezensis and application thereof in wheat sheath blight control and growth promotion
  • Bacillus velezensis and application thereof in wheat sheath blight control and growth promotion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Veles bacillus ( Bacillus velezensisi ) Isolation, screening and identification of M6:

[0029] (1) Separation and screening: Collect soil from the greenhouse in Baqiao District, Shaanxi Province, and bring it back with a fresh-keeping bag;

[0030] Weigh 10g of soil sample, put it into a conical flask filled with 90mL of sterile water, which contains 30 glass beads, vibrate at 37°C and 180rpm for 30min, and obtain a soil suspension after standing still;

[0031] Dilute the soil suspension to 10 -5 、10 -6 、10 -7 100µL of soil suspension with different dilutions was drawn and spread on the NA medium, cultured at 37°C for 24 hours, and single colonies of bacteria with different colors and shapes were picked, purified and preserved;

[0032] The antibacterial activity of isolated bacteria against plant pathogenic fungi was determined by plate confrontation method. The strawberry cinerea, tomato cinerea and cucumber cinerea were respectively activated on...

Embodiment 2

[0042] Embodiment 2: Veles bacillus ( Bacillus velezensisi ) Evaluation of the control effect of M6 fermentation broth on wheat sheath blight:

[0043] The Veles bacillus ( Bacillus velezensisi ) M6 was inoculated on a nutrient agar plate medium, and cultivated in a 37°C incubator for 24 hours;

[0044] The activated strain M6 was inoculated in liquid LB medium, cultured at 37°C, 230rpm for 18h;

[0045] The obtained bacterial strain M6 fermentation broth was centrifuged at 10,000 rpm and centrifuged for 6 minutes to remove the bacterial cells to obtain the supernatant of the fermentation broth;

[0046] The wheat sheath blight was activated on the PDA plate medium, the culture condition was 28°C, and the culture was 5 days;

[0047] Take wheat leaves that have grown for about 10 days, wash them with tap water, disinfect them with sodium hypochlorite for 1 minute, rinse them with sterilized distilled water for 3 times, wrap the roots with wet absorbent cotton and place them...

Embodiment 3

[0050] Embodiment 3: Veles bacillus ( Bacillus velezensisi ) M6 growth-promoting potential assessment:

[0051] The Veles bacillus ( Bacillus velezensisi ) M6 was inoculated on a nutrient agar plate medium, and cultivated in a 37°C incubator for 24 hours;

[0052] Detection of IAA (indole acetic acid) production: use the colorimetric method described by Patten et al., that is, inoculate the activated strain in LB liquid medium containing tryptophan, and ferment and culture. After the culture, take 1 mL of supernatant and add 4 mL of Salkowski 'sreagent (150mL concentrated sulfuric acid, 250mL water, 7.5mL 0.5M FeCl 3 .6H 2 O), placed at room temperature for 20min, observed the color change, when IAA was produced, the supernatant turned red.

[0053] Phosphorus dissolving ability test: Antagonistic endophytes were inoculated in Pikovskaya`s (PVK) medium for phosphate solubilization detection, and cultured at 28°C for 2 days. The phosphorus dissolving ability of the stra...

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Abstract

The invention relates to bacillus velezensis and an application thereof in wheat sheath blight control and growth promotion. The strain is bacillus velezensis M6, is preserved in China General Microbiological Culture Collection Center, has the preservation number of CGMCC No.15628 and can produce indoleacetic acid (IAA), siderophore and other growth-promoting substances; at the same time, the strain also has a significant effect of dissolving organic phosphorus; in addition, the strain has a good control effect on wheat sheath blight caused by rhizoctonia solani.

Description

technical field [0001] The present invention relates to a kind of Bacillus Velez, in particular to a kind of Bacillus Velez and its application in preventing and promoting the growth of wheat sheath blight. Background technique [0002] Plant fungal diseases are ubiquitous in food and vegetable crops. Food crop diseases caused by fungi have caused global food security problems. If the spread of fungal diseases in major food crops can be controlled, 125 million tons can be reduced annually globally. Reduce economic losses by more than 60 billion. Wheat is a gramineous grain crop widely planted all over the world. Its total output ranks second in the world. It plays a pivotal role in the food security system and agricultural production. There are many fungal diseases of wheat, including sheath blight, root Rot, take-all, head blight, powdery mildew, stripe rust, etc. According to statistics, wheat diseases can generally reduce wheat production by 10% to 15%, and serious ones ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C09K17/32C05F11/08C12R1/07C09K101/00
CPCA01N63/00C09K17/32C05F11/08C09K2101/00C12R2001/07C12N1/205
Inventor 安超薛文娇马赛箭刘晨丁浩
Owner MICROBIOLOGY INST OF SHAANXI
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