Composting microbial inoculant

A decomposing inoculum and composting technology, which is applied to bacteria, fungi, organic fertilizers, etc., can solve the problems that microorganisms cannot be quickly revived, it is not easy for straws to decompose quickly, and the activity of enzymes produced by microorganisms is low, so as to shorten the composting cycle and achieve excellent practical performance. , the effect of promoting degradation and humification

Inactive Publication Date: 2019-04-26
广东高龙环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, straw composting is not thoroughly decomposed when made into organic fertilizer, resulting in low activity of fungi, inability to quickly revive and colonize microorganisms, low enzyme activity of microorganisms, and difficulty in quickly decomposing straw. It needs to be improved, and there are deficiencies that cannot meet the needs of the society. development needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The preparation method of Aspergillus fumigatus is:

[0032] Seed medium: PDA medium;

[0033] Fermentation medium: potato 120kg / T, 10kg / T soluble starch, sucrose 15kg / T, corn flour 5kg / T, (NH 4 ) 2 SO 4 5kg / T, MgSO 4 ·7H 2 O 0.5kg / T, KH 2 PO 4 1kg / T, pH 6.0;

[0034] Fermentation conditions: 1% of the inoculum was inoculated into a 5 ton fermenter, the fermentation parameters were: 37°C, natural pH, tank pressure 1Kg, aeration rate of 90 cubic meters / hour, dissolved oxygen 40%, stirring 90rpm; fermentation time was 48h About; after the fermentation, the dry hyphae is important to reach more than 8g / L.

[0035] The preparation method of Saccharomyces cerevisiae is:

[0036] Seed medium: YPDA medium;

[0037] Medium: peptone 10kg / T, sucrose 8kg / T, glucose 2kg / T, KH 2 PO 4 1kg / T, MgSO 4 ·7H 2 O0.5kg / T, NaCl 5kg / T, pH 7.0-7.2;

[0038] Fermentation conditions: 37℃, pH 7.0-7.2, tank pressure 0.05MPa, aeration volume of 10-15 cubic meters per hour, fermentation time is about 24 hour...

Embodiment 1

[0049] Aspergillus fumigatus, Saccharomyces cerevisiae, Bacillus subtilis, and Bacillus cereus are fermented according to the above-mentioned medium and culture conditions to obtain a single inoculum with the required bacterial count.

[0050] For the piles of cow manure and straw with high lignocellulose content, mix according to the ratio of Bacillus subtilis: Bacillus cereus: Saccharomyces cerevisiae: Aspergillus fumigatus = 2:1:1:1:2.

[0051] The auxiliary materials are mixed according to the ratio of rice bran (straw powder): plant ash: kaolin: wheat bran = 7:0.5:1:1.5, and the moisture content is controlled at 10%.

[0052] According to the mixed bacterial liquid: base material = 2:8 (mass ratio), mix evenly, and control the moisture content at 30%.

[0053] Inoculate the prepared decomposing inoculants into the compost material at a ratio of 5‰ and mix them evenly. The fermentation temperature is controlled at 60°C for fermentation.

Embodiment 2

[0055] Aspergillus fumigatus, Saccharomyces cerevisiae, Bacillus subtilis, and Bacillus cereus are fermented according to the above-mentioned medium and culture conditions to obtain a single inoculum with the required bacterial count.

[0056] For the piles of cow manure and straw with high lignocellulose content, mix according to the ratio of Bacillus subtilis: Bacillus cereus: Saccharomyces cerevisiae: Aspergillus fumigatus = 2:1:1:1:2.

[0057] The auxiliary materials are mixed according to the ratio of rice bran (straw powder): plant ash: kaolin: wheat bran = 7:0.5:1:1.5, and the moisture content is controlled at 8%.

[0058] According to the mixed bacterial liquid: base material = 2:8 (mass ratio), mix evenly, and control the moisture content at 20%.

[0059] Inoculate the prepared decomposing bacterial agent into the compost material at a ratio of 3‰ and mix it evenly. The fermentation temperature is controlled at 65°C for fermentation.

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Abstract

The invention discloses a composting microbial inoculant. The composting microbial inoculant is prepared from aspergillus fumigatus, saccharomyces cerevisiae, bacillus subtilis and bacillus cereus. The composting microbial inoculant is prepared by uniformly mixing an inoculant with ingredients, inoculating a mixture into a composting material according to the inoculation proportion of 0.3 to 0.5 percent, uniformly mixing, controlling the fermentation temperature at 55 to 65 DEG C and finishing fermenting in seven days. By inoculating high temperature composting microorganisms in the process ofstraw / livestock and poultry manure composting (aerobic fermentation), the heating of a compost body can be promoted, the degradation and humification of lignocellulose are promoted, and crop straws,manures and the like can be quickly decomposed; straw compost also achieves complete composting, so that the aims of shortening the composting cycle and improving the composting quality are achieved;the composting microbial inoculant is a good innovative scheme and has a good marketing prospect.

Description

Technical field [0001] The invention relates to the technical field of microbial preparations, in particular to a compost decomposed fungus. Background technique [0002] The use of crop straws and livestock manure to ferment to produce bio-organic fertilizer plays an important role in realizing the reuse of waste resources and protecting the ecological environment. However, crop stalks are mainly composed of cellulose, semi-fiber, and lignin, and are slow to decompose. After use, they will affect soil physical properties and field cultivation, thus limiting the utilization of straw, an important organic resource. Exploring a straw biological decomposing agent can quickly decompose crop straws, improve the compost maturity and efficiency of straw compost, and apply it to farmland after making organic fertilizer, which has very important practical significance for promoting the sustainable development of agriculture. At present, when straw compost is made into organic fertilizer,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/18C12N1/20C05F11/08C12R1/68C12R1/865C12R1/125C12R1/085
CPCC12N1/14C12N1/18C12N1/20C05F11/08
Inventor 姜蒙钟诚雷雨曾伟张文荟李伍新
Owner 广东高龙环保科技有限公司
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