317 results about "Aspergillus fumigatus" patented technology

The present invention is directed to an isolated nucleic acid molecule encoding mutant phytases and the isolated mutant phytases themselves. The present invention further relates to methods of using the isolated nucleic acid molecules and the isolated mutant phytases of the present invention.

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cyptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

The present invention is directed to an isolated nucleic acid molecule encoding mutant phytases and the isolated mutant phytases themselves. The present invention further relates to methods of using the isolated nucleic acid molecules and the isolated mutant phytases of the present invention.

The present invention provides nucleotide sequences, methods and compositions that enable the experimental determination as to whether any gene in the genome of Aspegillus fumigatus is essential, and whether that gene is required for virulence or pathogenicity. The methods involve the construction of genetic mutants in which a target gene is placed under conditional expression. The identification of essential genes and those genes critical to the development of virulent infections, provides a basis for the development of screens for new drugs against Aspergillus fumigatus. The present invention further provides Aspergillum fumigatus genes that are essential and are potential targets for drug screening. The nucleotide sequence of the target genes can be used for various drug discovery purposes, such as expression of the recombinant protein, hybridization assay and construction of nucleic acid arrays. The uses of proteins encoded by the essential genes, and genetically engineered cells comprising modified alleles of essential genes in various screening methods are also encompassed by the invention.

A biochip for detecting the pathogenic funguses features that the DNA probes respectively for detecting one of 20 pathogenic funguses including Candida albicans, Aspergillus fumigatus, etc are immobilized on the glass plate, silicon chip, or high-molecular material.

The invention relates to a biological compound flocculant and an application thereof. The biological compound flocculant comprises a microbial flocculant and a modified chitosan solution, wherein the microbial flocculant is generated by fermenting aspergillus fumigatus HHE-A8, the aspergillus fumigatus is collected by the China center for type culture collection (CCTCC), the collection number is CCTCC NO: M 2012081, and the collection date is March 14th, 2012. When the biological compound flocculant and polymeric aluminum chloride are used in a compound mode, flocculation effects are good, the usage amount of the flocculant can be substantially reduced, and aluminiferous concentration of effluent is low, so that the biological compound flocculant is a water treatment agent with good performances and can be used for treating domestic sewage, industrial sewage and the like.

The invention discloses a fluorescence quantitative PCR (polymerase chain reaction) universal premier for detecting aspergillus, detection probes and a detection kit, wherein the detection kit comprises universal premiers displayed by base compositions such as SEQ ID. NO: 1 and SEQ ID. NO: 2; and the detection probe is CY5-TAAAGTTGGGTGTCGGCTGG-BHQ aiming at aspergillus fumigatus, the detection probe is FAM-TTGATTTGCGTTCGGCAAGC-BHQ aiming at aspergillus flavus, the detection probe is HEX-ACAAGTTGCAAATAAATGCGTCG-BHQ aiming at aspergillus, and / or the detection probe is ROX-ATGGTTGGAAAACGTCGGCA-BHQ aiming at aspergillus Niger. The multiple PCR detection method, premier, the probes and the kit thereof provided by the invention aim at four main pathogenic aspergilli and have high specificity and sensitivity; and the detection method is rapid, simple and convenient, and can be used for detecting and identifying the strain of pathogenic bacteria.

The invention discloses a compound biological flocculant as well as a preparation method and application thereof. The compound biological flocculant is prepared by compounding a microbial flocculant and a natural polymer anion modified flocculant; the natural polymer anion modified flocculant is obtained by modifying gel-contained wood flour through carboxymethyl; the microbial flocculant is generated through fermentation of aspergillus fumigatus HHE-A8; and the aspergillus fumigatus is collected by the China Center for Type Culture Collection (CCTCC for short), the collection number of the aspergillus fumigatus is CCTCC NO.M2012081, and the collection data of the aspergillus fumigatus is March 14, 2012. When the prepared compound biological flocculant is compounded with aluminum polychlorid for use, the flocculation effect is good, the dosage of the flocculant can be greatly decreased, and the aluminiferous concentration of effluent is low; and the compound biological flocculant can be used for treating tobacco wastewater, alga wastewater, ore washing wastewater, starch wastewater and the like.

The current invention relates to the field of detection and identification of clinically important fungi. More particularely, the present invention relates to species specific probes originating from the Internal Transcribed Spacer (ITS) region of rDNA for the detection of fungal species such as Candida albicans, Candida parapsilosis, Candida tropicalis, Candida kefyr, Candida krusei, Candida glabrata, Candida dubliniensis, Aspergillus flavus, Aspergillus versicolor, Aspergillus nidulans, Aspergillus fumigatus, Cryptococcus neoformans and Pneumocystis carinii in clinical samples, and methods using said probes.

The invention provides a microbe microbial inoculum and a preparation method thereof as well as a method for producing biological humic acid with sugarcane sugaring residue by the microbe microbial inoculum. The microbe microbial inoculum prepared by the invention comprises a culture medium and thallus, wherein the thallus comprises Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Streptomyces coelicolor and Aspergillus fumigatus, and each gram of compounded microbe microbial inoculum contains 0.5-1.5*109 total viable bacteria. The microbe microbial inoculum of the invention can quickly ferment sugarcane sugaring residues so as to obtain biological humic acid. Compared with mineral humic acid, the biological humic acid has the advantages of small molecular weight, large amount of active group, favourable water solubility, high bioactivity and the like and is easy to adsorb by the tissues of plants and animals, so that the biological humic acid can serve as the repairing preparation and fertilizer of water body and soil. Thus, the sugarcane sugaring residue can be effectively processed and utilized so as to really change waste material into things of value.

Fungal glucoamylases from Aspergillus fumigatus - expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

The present invention relates to extracellular polypeptides of Aspergillus fumigatus, to fragments of these polypeptides, to compositions comprising such polypeptides and fragments and to exposed domains and epitopes of these polypeptides. The invention also relates to the use of these polypeptides and fragments for immunisation and for production of antibodies, and to antibodies that recognise and bind the polypeptides. Furthermore, the invention relates to methods of identifying binding partners and inhibitors, and to methods of preventing, treating and diagnosing Aspergillus infections.

The invention relates to a preparation method of a galactomannan antigen. The method comprises the steps that breaking, centrifugation, alcohol precipitation, washing and drying are carried out on a living body rich in galactomannan, and galactomannan crude extract is obtained through separation; hydrazinolysis and hydrolysis are carried out on the obtained galactomannan crude extract in sequence; an enzyme is added to a hydrolysis product for enzymolysis, and the enzyme and small molecular substances in an enzymolysis product are removed; a product is purified through an ion exchange column, an affinity column and gel chromatography, and the pure galactomannan antigen is obtained; the obtained pure galactomannan antigen is identified. The galactomannan antigen obtained through the preparation method is high in purity, the inference of galactosamine, protein, miscellaneous sugar possibly included in the galactomannan crude extract is eliminated, and the preparation method can be used for preparing aspergillus fumigatus antibodies.

The invention relates to a methyl asterrate preparation method and the application thereof. The solid fermentation is performed to Fungi Geomyces sp, chemical compound methyl asterrate can be obtained by performing purification to fermentation products, and the chemical compound methyl asterrate prepared by the invention is confirmed methyl asterrate by applying technologies such as mass spectrum, nuclear magnetic resonance spectrum and infrared spectrum and documents. The invention also provides usages of the chemical compound in preparing drugs which can restraint the activity of aspergillus fumigatus.

The invention provides a recombinant thermostable chitosanase-producing engineered Pichia pastoris GS115 / HBCSN and a production method of thermostable chitosanase. The invention optimizes Aspergillus fumigatus chitosanase gene (GenBank accession number AY190324) based on the codon bias of Pichia pastoris, and the optimized nucleic acid sequence shows a 70% homology to the original nucleic acid sequence. The chitosanase produced by the recombinant Pichia pastoris can be secreted out of cells in a soluble form, with expression level of 3 mg / mL, and enzyme activity of 25,000U / mL. The chitosanase after glycosylation modification in Pichia pastoris has excellent thermal stability, which is proven by that the residual activities after treating at 80DEG C for 20 min, treating at 90DEG C for 40 min and treating at 100DEG C for 20 min are 56%, 87% and 87% respectively. The inventive thermostable chitosanase is especially suitable for large-scale industrial degradation of chitosan and environmental pollution reduction.

The invention discloses processing technology of Liupao tea. The technology is characterized in that in a pretreatment process of tea, a ZnO / TiO2 composite nanometer photocatalysis material and an ultraviolet light lamp are matched in order to carry out treatment for tea, and residual pesticides in the tea are degraded; a degrading microorganism agent is added into a piling process, biological enzyme produced from Aspergillus fumigatus, bacillus subtilis and Escherichia coli further degrades residual pesticides which are not degraded in tea; an ultraviolet ray dryer is used for drying in a drying process in order to kill bacteria, fungi and other microorganisms in tea, and provide a processing environment without competitors for subsequent pile fermentation and aging, and at the same time, ultraviolet ray further degrades residual pesticides. The processing technology of Liupao tea can be used for removing residual pesticides, and at the same time the Liupao tea also has 'aroma, heavy flavor, aging, and mellow flavor' with high quality.

The combination of pentraxin PTX3 with antifungal agents is described for the treatment of fungal infections and particularly for infections caused by Aspergillus fumigatus. Thanks to the synergistic effect of the two drugs, the combination allows the administration of suboptimal doses of antifungal agent.

The invention discloses a compound fungicide FX for degrading straws. The compound fungicide FX comprises a Bacillus subtilis strain NJX501, a Bacillus subtilis strain NJX502, a Bacillus methylotrophicus strain NJXy and an Aspergillus fumigatus strain NJZ501. The compound fungicide is prepared by mixing the bacterium liquid of NJX501 with a bacterium OD value of 0.5-1, the bacterium fluid of NJX502 with a bacterium OD value of 0.5-1, the bacterium liquid of NJXy with a bacterium OD value of 0.5-1 and NJZ501 spore suspension with spore number of not less than 104 / ml in equivalent volume. The compound fungicide FX can be used for promoting the composting and decomposition of rice straws under high temperature conditions.

The invention describes the use of a biomarker pentylfuran to detect bacterial and / or fungal pathogens. Pentylfuran is released from certain pathogens and detected in the headspace gas of an in vitro culture or in the breath sample of a patient. Pentylfuran is particularly useful in the detection of Aspergillus species, especially Aspergillus fumigatus which is a pathogen of humans.

The invention discloses a method for producing thallus feed by white spirit distiller's grain, which comprises the following steps of: preprocessing the distiller's grain; evenly mixing the distiller's grain with water at the mass ratio of (1:1)-(1:2); inoculating aspergillus fumigatus seed nutrient solution at the mass ratio of 2-6%; fermenting raw material; culturing for 3-7 days under the condition of 15-30% of air quantity and the temperature of 42-48DEG C; preparing a solid culture medium: evenly mixing 25-65 parts of preprocessed distiller's grain and 5-10 parts of cane sugar or waste molasses by tap water at the material and liquid ratio of (1:1)-(1:1.5); activating a fermented strain under a total-nutrient culture medium; carrying out enrichment culture; inoculating the fermented seed nutrient solution onto the solid culture medium, and fermenting; mixing the fermented solid distiller's grain fermented by saccharomycetes with solid distiller's grain fermented by lactic acid bacteria at any ratio; and drying at 60-70DEG C, granulating and packaging. According to the method for producing the thallus feed by the white spirit distiller's grain, the resource of protein feed can be expanded, and the use ratio of the solid white spirit distiller's grain can be improved.

The invention discloses an anti-bacterial mould-proof floor film and a preparation technology thereof. The floor film is prepared from the following raw materials in parts by weight: 80-100 parts of PVC resin powder, 2.5-4 parts of a silver-carrying nano TiO2 antibacterial agent, 2-3 parts of a mildew preventive, 12-16 parts of epoxidized soybean oil, 5-7 parts of a plasticizer and 3-5 parts of a stabilizer. The raw materials of the floor film are added with the silver-carrying nano TiO2 antibacterial agent with good antibacterial property, breeding of microorganisms such as bacteria can be effectively inhibited, and a floor is prevented from being eroded by the bacteria; and the mildew preventive in the raw materials is a compound of pyridine sulfur-copper-zinc and N-octyl-4-isothiazolin-3-one, can inhibit growth of gram positive and negative bacteria, penicillium purpurogenum and aspergillus fumigatus and has excellent mildew-proof property.

The invention provides a preparation method of burn-free tourmaline biological ceramsite and an application thereof in decolouration of dye wastewater. The preparation method disclosed by the invention comprises the following steps: (1), weighing the following components according to a formula: 37.5-46.5% of coal ash, 12-17% of cement, 12-17% of lime powder, 12-17% of sodium bicarbonate, 1-2% of gypsum, 3-7% of water glass, 1-10% of tourmaline, and 0.5-2% of aspergillus fumigatus, wherein the sum of the percentages by weight of various components is 100%; (2), granulating: uniformly mixing various components to form a mixture, adding water into the mixture, wherein the addition amount of water is 30-40% of that of the mixture; uniformly stirring, and granulating to obtain raw ceramsite with the particle size of 3-8 mm; and (3), aging, drying and steaming the raw ceramsite at constant temperature to obtain the burn-free tourmaline biological ceramsite. The burn-free tourmaline biological ceramsite prepared by the preparation method provided by the invention has the characteristics of being low in cost and good in decolouration property.

The invention discloses a lipase and a gene thereof, a recombinant expression vector, recombinant expression transformant and recombinase containing the gene, and a preparation method of the recombinase. In addition, the invention also discloses application of thallus containing the recombinant lipase as a catalyst in preparing an optically active substance from alpha-acetoxyphenylacetic acid and2-Cl-alpha-acetoxyphenylacetic acid in an asymmetric deacylation mode. The recombinant lipase gene is derived from aspergillus fumigatus Af293. The recombinase can be used as a catalyst for asymmetric deacylation to prepare optically active mandelic acid. The recombinase has the advantages of high catalytic efficiency, strong stereoselectivity, mild applicable reaction conditions and environmental friendliness. The recombinase disclosed by the invention has high catalytic activity, and has wide prospects in industrial application and development.

The invention discloses a 5,5,6-polycyclic tetramic-acid containing macrocyclic lactam compound and a preparation method and the application of the 5,5,6-polycyclic tetramic-acid containing macrocyclic lactam compound. The 5,5,6-polycyclic tetramic-acid containing macrocyclic lactam compound can be used as a tumor cell proliferation inhibitor or a tumor cell killer or an aspergillus fumigatus growth inhibitor.

The invention discloses a composting microbial inoculant. The composting microbial inoculant is prepared from aspergillus fumigatus, saccharomyces cerevisiae, bacillus subtilis and bacillus cereus. The composting microbial inoculant is prepared by uniformly mixing an inoculant with ingredients, inoculating a mixture into a composting material according to the inoculation proportion of 0.3 to 0.5 percent, uniformly mixing, controlling the fermentation temperature at 55 to 65 DEG C and finishing fermenting in seven days. By inoculating high temperature composting microorganisms in the process ofstraw / livestock and poultry manure composting (aerobic fermentation), the heating of a compost body can be promoted, the degradation and humification of lignocellulose are promoted, and crop straws,manures and the like can be quickly decomposed; straw compost also achieves complete composting, so that the aims of shortening the composting cycle and improving the composting quality are achieved;the composting microbial inoculant is a good innovative scheme and has a good marketing prospect.

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

The invention belongs to the technical field of grain storage, and discloses a bacillus subtilis strain having broad-spectrum antagonism for main grain storage hazard-moulds, a preparation method of an antifungal metabolite of the bacillus subtilis strain, and application of the bacillus subtilis strain and the antifungal metabolite in grain storage. The strain is bacillus subtilis LM3403, and has a preservation number of CGMCC No. 9377. The bacillus subtilis LM3403 has a better inhibiting effect for the main grain storage hazard-moulds such as fusarium graminearum, fusarium moniliforme, aspergillus flavus, aspergillus niger, aspergillus glaucus, aspergillus fumigatus, penicillium oxalicum, penicillium expansum and mucor mucedo, and is wide in antibacterial spectrum, thus having a giant application potential. The antifungal metabolite of the bacillus subtilis LM3403 has an obvious inhibiting effect for the growth of the main grain storage hazard-moulds. The storage test results prove that after the antifungal metabolite of the bacillus subtilis LM3403 is added into stored grains, the growth of the moulds can be effectively inhibited in a grain storage process, so that the grain storage safety is guaranteed.

The invention discloses a primer group for detecting respiratory tract microorganisms based on a nanopore sequencing method. The nucleotide sequences of the primer group are as shown in SEQ ID NO:1-20. The microorganisms are streptococcus pneumoniae, staphylococcus aureus (resisting to methicillin), klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, candida albicans, haemophilus influenzae, legionella pneumophila, enterococcus faecium, chlamydia psittaci, cryptococcus gattii, aspergillus fumigatus and pneumocystis jiroveci. According to the invention, sequencing optimization is carried out on different types of samples of the respiratory tract microorganisms, so that the detection method, the kit and the like are suitable for various typesof respiratory tract samples; and a targeted amplification method is adopted, so that the detection requirements of sputum, alveolar lavage and other sample types can be met. In addition, parallel sequencing can be performed, so that the flux of the detected samples is increased, the sequencing time is shortened, and the contradiction between the flux of detected pathogenic species and the cost and time is further relieved.

The invention relates to the field of biologically-derived insecticidal preparations, in particular to a complex microbial inoculant for controlling root-knot nematodes of crops and a preparation method of the complex microbial inoculant. The complex microbial inoculant for controlling the root-knot nematodes of the crops and the preparation method of the complex microbial inoculant are characterized in that microorganism is composed of bacillus subtilis, bacillus sphaericus, pseudomonas fluorescens, bacillus thuringiensis, brevibacillus laterosporus, paenibacillus polymyxa, pasteurella pneumoniae, paecilomyces lilacinus, verticillium chalamydosporium, beauveria bassiana and aspergillus fumigatus. The novel multi-bacteria same-pot fermentation technology is adopted, the formula is clever and reasonable, the disadvantages of mutual antagonism in the liquid deep fermentation culture of compound bacteria are eliminated, the compound bacteria has high activity, and the complex microbial inoculant for controlling the root-knot nematodes of the crops has a good control effect on tomatoes and cucumber root-knot nematodes.

The invention relates to aspergillus fumigatus and application thereof. The collection number of aspergillus fumigatus LZ2 is CGMCC No.3859. The aspergillus fumigatus LZ2 CGMCC No.3859 can grow by taking imazethapyr as a unique carbon source, and is cultured in a basic salt culture medium which contains 200 mg.L-1 of imazethapyr for 8 days, so that the degradation rate of imazethapyr is up to 82.91 percent, and technical support is provided for the treatment of imazethapyr-polluted soil and water bodies. Meanwhile, the aspergillus fumigatus LZ2 CGMCC No.3859 is cultured in soil which contains 100 mug.kg-1 of imazethapyr for 60 days, so that the degradation rate of imazethapyr is up to 76.84 percent which is far higher than the imazethapyr degradation rate of natural soil, and the degradation half-life period of imazethapyr in soil is advanced from 75.9 days of natural degradation to 30.9 days by 48.6 days. By adopting the aspergillus fumigatus LZ2 CGMCC No.3859, degradation of imazethapyr in soil can be accelerated effectively.