Method for producing thallus feed by white spirit distiller's grain

A technology for the production of liquor distillers grains and bacteria, applied in animal feed, animal feed, applications, etc., can solve the problems of nutritional structure defects, low digestion utilization rate, environmental pollution, etc., and achieve the effect of expanding sources and improving utilization rate

Active Publication Date: 2012-11-21
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, due to the special production process, the acidity of discarded grains is high, and it is easy to spoil and deteriorate. If it is not treated in time, it will inevitably seriously pollute the environment.
The use of distiller's grain umami feed has the disadvantages of nutritional structure defects and low digestibility, and it is difficult to store and transport

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for producing bacterial feed from liquor distillers grains, comprising the following steps:

[0021] (1) Pretreatment of distiller's grains: Mix 25g of distiller's grains with 25g of water evenly, inoculate 1ml of Aspergillus fumigatus seed culture solution, and ferment the raw meal. The ventilation rate is 25-30%, and cultivated at 45-48°C for 7 days;

[0022] (2) Preparation of solid medium: 80g of pretreated distiller's grains, 20g of sucrose, mixed with 100g of tap water;

[0023] (3) Solid distiller's grains fermented by yeast: the strains are activated in the full nutrient medium, enriched for 24-36 hours, and 1% (v / w) inoculum is inserted into the solid medium for 15 days of fermentation at 28°C;

[0024] (4) Dry the solid distiller's grains fermented by the above-mentioned fermented yeast at 70°C, use a disc granulator to granulate, put the weighed material in the disc granulator, start the granulator, adjust The speed of the granulator and the inclina...

Embodiment 2

[0027] A method for producing bacterial feed from liquor distillers grains, comprising the following steps:

[0028] (1) Pretreatment of distiller's grains: Mix 250g of distiller's grains with 375g of water evenly, inoculate 25ml of Aspergillus fumigatus seed culture solution, and ferment the raw meal. The ventilation rate is 20-30%, and cultivated at 42-45°C for 7 days;

[0029] (2) Preparation of solid medium: 6500g of pretreated distiller's grains, 100g of waste molasses, mixed with 9900g of tap water;

[0030] (3) Solid distiller's grains fermented by lactic acid bacteria: the strains are activated in the full nutrient medium, enriched for 24-36 hours, and the lactic acid bacteria are inserted into the solid medium at 32°C for 5 days at 5% (v / w) inoculum;

[0031] (4) Dry the fermented solid distiller's grains fermented by lactic acid bacteria above at 60°C, use a disc granulator to granulate, put the weighed material in the disc granulator, start the granulator, and adjus...

Embodiment 3

[0034] A method for producing bacterial feed from liquor distillers grains, comprising the following steps:

[0035] (1) Pretreatment of distiller's grains: Mix 200g of distiller's grains with 200g of water evenly, inoculate 0.8ml of Aspergillus fumigatus seed culture solution, and ferment the raw material with ventilation rate: 15%, culture at 45°C for 3 days;

[0036] (2) Preparation of solid medium: mix 160g of pretreated distiller's grains and 40g of sucrose with 300g of tap water;

[0037] (3) Yeast fermenting solid distiller's grains: the strains are activated under the full nutrient medium, enriched for 24-36 hours, and the yeast is inserted into the solid medium at 35°C for 7 days at 6% (v / w) inoculum;

[0038] (4) Solid distiller's grains fermented by lactic acid bacteria: the strains are activated in a complete nutrient medium, enriched for 24-36 hours, and 3% (v / w) inoculum of lactic acid bacteria is inserted into the solid medium for 2 days of fermentation at 35°C;...

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Abstract

The invention discloses a method for producing thallus feed by white spirit distiller's grain, which comprises the following steps of: preprocessing the distiller's grain; evenly mixing the distiller's grain with water at the mass ratio of (1:1)-(1:2); inoculating aspergillus fumigatus seed nutrient solution at the mass ratio of 2-6%; fermenting raw material; culturing for 3-7 days under the condition of 15-30% of air quantity and the temperature of 42-48DEG C; preparing a solid culture medium: evenly mixing 25-65 parts of preprocessed distiller's grain and 5-10 parts of cane sugar or waste molasses by tap water at the material and liquid ratio of (1:1)-(1:1.5); activating a fermented strain under a total-nutrient culture medium; carrying out enrichment culture; inoculating the fermented seed nutrient solution onto the solid culture medium, and fermenting; mixing the fermented solid distiller's grain fermented by saccharomycetes with solid distiller's grain fermented by lactic acid bacteria at any ratio; and drying at 60-70DEG C, granulating and packaging. According to the method for producing the thallus feed by the white spirit distiller's grain, the resource of protein feed can be expanded, and the use ratio of the solid white spirit distiller's grain can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing bacterial feed from liquor distiller's grains. [0002] Background technique [0003] In recent years, the high development of animal husbandry has greatly increased the demand for protein feed, and the serious shortage of protein feed has become one of the important problems that the feed raw material industry needs to solve urgently. [0004] Research on the production of protein feed from distiller's grains, a by-product of winemaking, has always been a hot spot in the feed industry. [0005] Solid liquor waste is the main by-product of liquor production, and it is also the main pollutant of liquor production. According to statistics, the amount of discarded grains is 4-8 times that of liquor production. At present, only China needs to deal with more than 50 million tons of discarded grains. Because raw materials such as sorghum and wheat are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/06A23K10/38
CPCY02P60/87
Inventor 邱树毅王晓丹
Owner GUIZHOU UNIV
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