Aspergillus fumigatus and application thereof to degradation of imazethapyr
A technology of imazethapyr and Aspergillus fumigatus, which is applied in the direction of microorganism-based methods, restoration of contaminated soil, water/sludge/sewage treatment, etc., can solve the problems of less research on imazethapyr biodegradation bacteria, and achieve the effect of accelerating degradation
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Embodiment 1
[0027] Embodiment 1 The separation of Aspergillus fumigatus LZ2 of the present invention
[0028] 1. Isolation of strains
[0029] Retrieve the soil from a soybean field where imazethapyr has been applied for a long time on a farm in Nenjiang, Heilongjiang Province, mix these soils in an aseptic operating bench, weigh 2g, put it into a 250mL triangular flask filled with 100mL distilled water, seal it with a sterile sealing film, and store it at 200r min -1 Shake at the speed of 20 minutes, dilute properly to get the dilution, drop 1 drop of the dilution onto the Martin's medium plate containing imazethapyr, spread it flat with a triangular glass rod, and incubate it upside down in a biochemical incubator at 28°C. After 7 days, the strains with good growth were selected and separated by streaking on the Martin's medium plate, until pure bacteria were obtained, that is, multiple strains of imazethapyr-resistant fungi.
[0030] Make the spore suspension (10 6 CFU mL -1 ), acc...
Embodiment 2
[0037] Example 2 Application of Aspergillus fumigatus LZ2 of the present invention in degrading imazethapyr
[0038] 1. Degradation of imazethapyr by Aspergillus fumigatus LZ2
[0039] According to the amount of 2% (v / v), the spore suspension of LZ2 (10 6 CFU mL -1 ) to imazethapyr concentration of 200mg L -1 In the basal salt culture medium, the control sample was imazethapyr at a concentration of 200mg·L -1 basal salt culture solution, at 28°C, 140r·min -1 Shaker shaking culture. Samples were taken every day to determine the content of imazethapyr and the growth of bacteria by dry weight method. Such as Figure 4 shown. Depend on Figure 4 It can be seen that the lag period of LZ2 is 3 days, during this period, the degradation effect of LZ2 on imazethapyr is very weak; the logarithmic growth phase of LZ2 is the 3rd to 7th day, at this time the strain grows rapidly, metabolism is vigorous, and the degradation rate of imazethapyr is also low. After the 7th day, the gr...
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