Aspergillus fumigatus and application thereof to degradation of imazethapyr

A technology of imazethapyr and Aspergillus fumigatus, which is applied in the direction of microorganism-based methods, restoration of contaminated soil, water/sludge/sewage treatment, etc., can solve the problems of less research on imazethapyr biodegradation bacteria, and achieve the effect of accelerating degradation

Inactive Publication Date: 2012-01-25
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Studies have proved that microbial degradation is the main degradation pathway for imazethapyr residues in soil (Su Shaoquan. 1998. The safety of long-residual herbicides on subsequent crops [J]. Pesticides. 37 (12)), but the current research on the biodegradation bacteria of imazethapyr Fewer, and mostly focused on bacteria

Method used

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  • Aspergillus fumigatus and application thereof to degradation of imazethapyr
  • Aspergillus fumigatus and application thereof to degradation of imazethapyr
  • Aspergillus fumigatus and application thereof to degradation of imazethapyr

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Embodiment 1

[0027] Embodiment 1 The separation of Aspergillus fumigatus LZ2 of the present invention

[0028] 1. Isolation of strains

[0029] Retrieve the soil from a soybean field where imazethapyr has been applied for a long time on a farm in Nenjiang, Heilongjiang Province, mix these soils in an aseptic operating bench, weigh 2g, put it into a 250mL triangular flask filled with 100mL distilled water, seal it with a sterile sealing film, and store it at 200r min -1 Shake at the speed of 20 minutes, dilute properly to get the dilution, drop 1 drop of the dilution onto the Martin's medium plate containing imazethapyr, spread it flat with a triangular glass rod, and incubate it upside down in a biochemical incubator at 28°C. After 7 days, the strains with good growth were selected and separated by streaking on the Martin's medium plate, until pure bacteria were obtained, that is, multiple strains of imazethapyr-resistant fungi.

[0030] Make the spore suspension (10 6 CFU mL -1 ), acc...

Embodiment 2

[0037] Example 2 Application of Aspergillus fumigatus LZ2 of the present invention in degrading imazethapyr

[0038] 1. Degradation of imazethapyr by Aspergillus fumigatus LZ2

[0039] According to the amount of 2% (v / v), the spore suspension of LZ2 (10 6 CFU mL -1 ) to imazethapyr concentration of 200mg L -1 In the basal salt culture medium, the control sample was imazethapyr at a concentration of 200mg·L -1 basal salt culture solution, at 28°C, 140r·min -1 Shaker shaking culture. Samples were taken every day to determine the content of imazethapyr and the growth of bacteria by dry weight method. Such as Figure 4 shown. Depend on Figure 4 It can be seen that the lag period of LZ2 is 3 days, during this period, the degradation effect of LZ2 on imazethapyr is very weak; the logarithmic growth phase of LZ2 is the 3rd to 7th day, at this time the strain grows rapidly, metabolism is vigorous, and the degradation rate of imazethapyr is also low. After the 7th day, the gr...

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Abstract

The invention relates to aspergillus fumigatus and application thereof. The collection number of aspergillus fumigatus LZ2 is CGMCC No.3859. The aspergillus fumigatus LZ2 CGMCC No.3859 can grow by taking imazethapyr as a unique carbon source, and is cultured in a basic salt culture medium which contains 200 mg.L-1 of imazethapyr for 8 days, so that the degradation rate of imazethapyr is up to 82.91 percent, and technical support is provided for the treatment of imazethapyr-polluted soil and water bodies. Meanwhile, the aspergillus fumigatus LZ2 CGMCC No.3859 is cultured in soil which contains 100 mug.kg-1 of imazethapyr for 60 days, so that the degradation rate of imazethapyr is up to 76.84 percent which is far higher than the imazethapyr degradation rate of natural soil, and the degradation half-life period of imazethapyr in soil is advanced from 75.9 days of natural degradation to 30.9 days by 48.6 days. By adopting the aspergillus fumigatus LZ2 CGMCC No.3859, degradation of imazethapyr in soil can be accelerated effectively.

Description

technical field [0001] The invention relates to a strain of Aspergillus fumigatus and its application. Background technique [0002] Imazethapyr, an imidazolinone herbicide developed by American Cyamide (now BASF) in the 1980s, is an inhibitor of acetolactate synthase (ALS) or acetate hydroxyacid synthase (AHAs). Because of its advantages such as wide herbicidal spectrum, strong selectivity, high activity, low dosage, low price, and good efficacy, it is widely used and has become one of the best herbicide varieties in soybean fields. It is conservatively estimated that imazethapyr single-dose sales are only about 3830t in Heilongjiang Province each year, and the usage area is 1.33x10 6 ~2x10 6 hm 2 (Geng Gui, Herbicide damage, non-point source pollution and preventive countermeasures in Heilongjiang Province, China Agricultural Science Bulletin Volume 24 Supplement, November 2008). [0003] The recommended dosage of imazethapyr is 75-100g hm -2 (active ingredient), it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34B09C1/10A62D3/02C12R1/68C02F101/38A62D101/04A62D101/28
Inventor 李淑芹曹知平许井钢
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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