The invention provides a preparation method of a
rabies inactivation vaccine for
livestock and a stabilizing agent contained in the vaccine. According to the preparation method, a
virus concentrationprocess is that (1) a
virus supernate continuously penetrates through a 300 KD membrane bag at the flow speed of 0.8-1 L / min, the
virus is concentrated until the volume of the virus is 1 / 50-1 / 40 of the original volume, and coarse concentration liquid is obtained; (2) a PBS solution with the volume 10-15 times larger than that of the coarse concentration liquid is added into the coarse concentration liquid, the mixture penetrates through the 300 KD membrane bag at the flow speed of 1.8-2 L / min under the concentration pressure of 0.5-1.0 bar, under the condition that the mixture is concentrateduntil the volume of the mixture is 1 / 4-2 / 5 of the original volume of the mixture before penetration, the flow speed is adjusted to 0.8-1 L / min, and continuous concentration is conducted until the volume of the mixture is the same as the original volume of the coarse concentration liquid. According to the preparation method, by optimizing the virus concentration process, different membrane-penetration flow speeds are adopted for different
antigen concentrations, in the concentration process, 30-50% of impure
protein can be removed, the virus
titer is kept at 90% or above, and the prepared vaccine is more stable and more efficient.