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Method for preparing pig porcine reproductive and respiratory syndrome inactivated vaccine

An inactivated vaccine, the technology of porcine PRRS, which is applied in the field of porcine PRRS inactivated vaccine preparation, can solve the problems of poor immune effect, high side reaction rate, difficult injection, etc., and achieve easy injection, high antigen content, and safety good sex effect

Inactive Publication Date: 2017-05-31
鼎正生物药业(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a preparation method of pig PRRS inactivated vaccine to solve the technical problems of high side reaction rate, poor immune effect, difficult injection and difficult absorption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of porcine PRRS inactivated vaccine, comprising virus clarification, concentration, molecular sieve chromatography, inactivation, freeze-drying and comprising the following steps:

[0021] 1) clarifying the Lelysta virus cell culture fluid;

[0022] 2) Concentrate the initially filtered Lelysta virus liquid by means of horizontal cutting and ultrafiltration concentration;

[0023] 3) using a molecular sieve chromatography column to purify the Lelysta virus liquid;

[0024] 4) using β-propiolactone to inactivate the Lelysta virus liquid;

[0025] 5) adding a suitable freeze-drying protective agent to the inactivated virus liquid to freeze-dry;

[0026] On the basis of the above technical solutions, the following conditions are met:

[0027] Step 1) After filtering with 8 layers of gauze, filter with a double-layer filter membrane of 0.5 μm.

[0028] The concentration factor of step 2) is 20 times.

[0029] The pore size of the cross-cut ultrafil...

Embodiment 2

[0032] A preparation method of porcine PRRS inactivated vaccine, comprising virus clarification, concentration, molecular sieve chromatography, inactivation, freeze-drying and comprising the following steps:

[0033] 1) clarifying the Lelysta virus cell culture fluid;

[0034] 2) Concentrate the initially filtered Lelysta virus liquid by means of horizontal cutting and ultrafiltration concentration;

[0035] 3) using a molecular sieve chromatography column to purify the Lelysta virus liquid;

[0036] 4) using β-propiolactone to inactivate the Lelysta virus liquid;

[0037] 5) adding a suitable freeze-drying protective agent to the inactivated virus liquid to freeze-dry;

[0038] On the basis of the above technical solutions, the following conditions are met:

[0039] Step 1) After filtering with 8 layers of gauze, filter with a 2 μm double-layer filter membrane.

[0040] The concentration factor of step 2) is 50 times.

[0041] The pore size of the cross-cut ultrafiltrati...

Embodiment 3

[0044] A preparation method of porcine PRRS inactivated vaccine, comprising virus clarification, concentration, molecular sieve chromatography, inactivation, freeze-drying and comprising the following steps:

[0045] 1) clarifying the Lelysta virus cell culture fluid;

[0046] 2) Concentrate the initially filtered Lelysta virus liquid by means of horizontal cutting and ultrafiltration concentration;

[0047] 3) using a molecular sieve chromatography column to purify the Lelysta virus liquid;

[0048] 4) using β-propiolactone to inactivate the Lelysta virus liquid;

[0049] 5) adding a suitable freeze-drying protective agent to the inactivated virus liquid to freeze-dry;

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PUM

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Abstract

The invention provides a method for preparing a pig porcine reproductive and respiratory syndrome inactivated vaccine. Through steps of virus clarification, concentration, molecular sieve chromatography, inactivation, freeze-drying and the like, the method is capable of effectively ensuring that the content of impurities in antigen for preparing the vaccine is reduced to the minimum extent, so that the phenomenon that side effects are caused after the product is used can be avoided, and meanwhile the inactivation effect of the vaccine can be ensured. The PRRSV inactivated vaccine prepared by using the method is high in antigen content, high in immunogenicity, high in antigen purity, good in security, small in side effect, free of adjuvant, rapid in antibody protection generation, easy to inject and easy to absorb.

Description

technical field [0001] The invention relates to the technical field of vaccine preparation, in particular to a method for preparing an inactivated vaccine against porcine blue-ear disease. Background technique [0002] Pig blue-ear disease is a highly contagious disease that is endemic. PRRSV only infects pigs, and pigs of various breeds, ages and uses can be infected, but pregnant sows and piglets within 1 month are most susceptible. Sick pigs and infected pigs are important sources of infection for this disease. The main routes of transmission are contact infection, airborne transmission and semen transmission, and can also be transmitted vertically through the placenta. Susceptible pigs can be infected with the virus through oral, nasal, intramuscular, intraperitoneal, intravenous and intrauterine inoculation, etc. The virus can be transmitted to other susceptible pigs through contact within 2 to 14 weeks after being infected with the virus. The virus can be detected i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12C12N7/00A61P31/14
Inventor 刘鼎阔王立红张凤洪张俊霞
Owner 鼎正生物药业(天津)有限公司
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