The invention relates to an improved
body fluid color-developing fungi 1,3-beta-D-
glucan detection kit, which comprises a reaction main-agent, a main
agent combination solution, a sample treatment fluid,
sterile water, a standard substance and a
quality control material. With
Tachypleus tridentatus Leach or
Limulus polyphemus Linnaeus
hemocyte lysate as a main
raw material, the reaction main-agent contains G factor, B factor, C factor, clottable
protein and a polypeptide
chromogenic substrate. By a new preparation process, the reaction main-agent has characteristics of high stability and small intra-
assay and inter-
assay difference. The main
agent combination solution adopts a new formula. After the main
agent combination solution is mixed with the reaction main-agent,
Tachypleus Amebocyte Lysate endotoxin reaction
branch can be effectively shielded. According to the kit, rate-method
enzyme kinetics detection is carried out by ELIASA. The detection speed is fast, and anti-interference performance is strong. In addition, the preparation technology is simple, and the product has stronger stability and higher specificity and sensitivity.