42results about How to "High antigen content" patented technology

A fowl adenovirus group I serum type 4 genetic engineering subunit vaccine, and a preparing method and applications thereof are disclosed. The sequence of an antigen protein in the vaccine is shown as SEQ ID NO:1. The antigen protein has advantages of high safety, high immunity, no pathogenicity for chickens or other animals, and the like. The subunit vaccine can prevent chicken hydropericardium syndrome, inclusion body hepatitis and other diseases which are caused by infection of fowl adenovirus group I serum type 4.

The invention relates to a PCV2 (porcine circovirus 2) ELISA (enzyme-linked immuno sorbent assay) antigen detection kit as well as a preparation method and applications thereof, wherein the detection kit comprises an elisa plate of a polyclonal antibody of peridium anti-PCV2-Cap (nucleocapsid) protein, seal liquids, sample diluent, an antigen standard product, a second antibody of a monoclonal antibody of HRP marked anti-PCV2-Cap protein, a concentrated washing liquid, an enzyme substrate solution A, an enzyme substrate solution B and a stop solution, wherein the antigen standard product is purified reconstructed PCV2-Cap protein. The specificity of the kit provided by the invention achieves 100%, and the sensitivity is as high as 4ng/ml, and the kit can be used for swinery PCV2 antigen detection and PCV2 vaccine product quantitative detection.

The invention relates to a 2 type subunit vaccine for a porcine circovirus as well as a preparation method and application thereof. A recombinant bacilliform virus contains double promoters (a polyhedrin promoter and a P10 promoter), a coding gene of a Cap protein with double copying can be expressed, and the expression efficiency of the protein is obviously enhanced; moreover, the Cap protein expressed by an inserted foreign gene does not contain an excess sequence, virus-like particles (VLPs) can be effectively formed, and the immunogenicity of an expressed protein is enhanced; furthermore, a produced antigen has high content; and according to the 2 type subunit vaccine for the porcine circovirus, which is disclosed by the invention, the productivity ratio and the quality of a viral protein of the 2 type subunit vaccine for the porcine circovirus are obviously enhanced, and a prepared vaccine composition has the advantages of stable and persistent immune effect, high safety and the like.

The invention relates to a preparation method of a porcine parvovirus inactivated vaccine. The preparation method comprises the following preparation steps of: (1) taking a porcine parvovirus fluid out of a raw material tank, placing the porcine parvovirus fluid into a microfiltration system, filtering to obtain a penetrating fluid and a concentrated solution, returning the concentrated solution to the raw material tank, and discharging the porcine parvovirus fluid after the solid content of the porcine parvovirus fluid is larger than or equal to 1g/L; (2) carrying out ultrafiltration treatment on the penetrating fluid, and further concentrating the porcine parvovirus fluid until the virus hemocoagulate value is up to 28-213 to obtain a concentrated solution; (3) filtering the concentrated solution again by using the microfiltration system to obtain a parvovirus fluid with the hemocoagulate value of 28-213 after the treatment; and (4) inactivating the parvovirus fluid and preparing the concentrated and inactivated parvovirus fluid into a vaccine product. The invention provides a safe and feasible vaccine production method so that vaccine production is not dependent on the traditional process again, high-titer parvovirus fluid is produced, the antigen content is increased, and the requirement for immune production is met.

The invention relates to a large-scale production method of a rotavirus vaccine, particularly a large-scale culture method of a rotavirus inactivated vaccine by using a 14L bioreactor. The method comprises the following steps: 1) revival and subculture of Vero cells; 2) cell collection and bioreactor inoculation; 3) bioreactor culture of cells; 4) solution exchange and cell washing; 5) rotavirus activation and infection; and 6) sustaining culture and collection of rotavirus. The invention aims to overcome the defect of low infectivity titer in the obtained virus since the rotavirus can not easily release cells in rotavirus vaccine large-scale production, thereby obtaining the high-titer rotavirus, and greatly enhancing the rotavirus yield. The invention also aims to overcome the defect of low adsorption infectivity of rotavirus in the mass production process, thereby greatly enhancing the rotavirus quality and further enhancing the antigenicity and immunogenicity of the rotavirus inactivated vaccine.

The present invention discloses a preparation method for a microencapsulated oral live vaccine of gosling plague. The preparation method comprises: preparing a gosling plague SYG strain into a vaccine half finished product by goose embryo proliferation culture; adding a 5% sucrose and skimmed milk solution after a sterility test is qualified, and uniformly stirring; adding 20-40% porous starch tothe half finished product solution, stirring for 20-40 minutes at a certain temperature, wherein the temperature is controlled to 37 DEG C; mixing the resulting liquid and a 1-2.5% sodium alginate solution, completely stirring, and then carrying out dehydration and drying by a fluidized bed to prepare the microencapsulated vaccine dry powder of the gosling plague, wherein the volume of the resulting liquid is the same as the volume of the sodium alginate solution. The method of the present invention ha characteristics of science, simpleness, stable and reliable production, less loss of vaccine titer. With adopting the vaccine microencapsulation technology, the live vaccine of the gosling plague can be adopted for immunization by the oral route, the stress reaction is reduced, the sustained release function is provided for the live vaccine of the present invention, and the breeder goose in the laying period can use the live vaccine as usual.

The invention provides a preparation method for human diploid cell rabies vaccine virus solution, and relates to the field of biotechnology. The preparation method comprises the step of inoculating rabies vaccine fixed virus PM-1503-3M strain in MRC-5 cell to generate the human diploid cell rabies vaccine virus solution. The method disclosed by the invention is simple in process and cost-saving; and the prepared virus solution is high in antigen content, high in virus valence and titer, suitable for large-scale industrialized production, and capable of meeting the needs of domestic and foreign markets on human diploid cell rabies vaccine.

The invention provides a method for preparing a pig porcine reproductive and respiratory syndrome inactivated vaccine. Through steps of virus clarification, concentration, molecular sieve chromatography, inactivation, freeze-drying and the like, the method is capable of effectively ensuring that the content of impurities in antigen for preparing the vaccine is reduced to the minimum extent, so that the phenomenon that side effects are caused after the product is used can be avoided, and meanwhile the inactivation effect of the vaccine can be ensured. The PRRSV inactivated vaccine prepared by using the method is high in antigen content, high in immunogenicity, high in antigen purity, good in security, small in side effect, free of adjuvant, rapid in antibody protection generation, easy to inject and easy to absorb.

The invention relates to duck hepatitis bivalent live vaccines and a preparation method thereof. According to the invention, 1) SPF chicken embryos are inoculated with vaccine strains of the duck hepatitis bivalent live vaccines, namely DHV-1 QL1 strains, through allantoic cavities for culture, and are inoculated with DHV-3 QL3 strains through chorioallantoic membranes or yolk sacs for culture, the antigen contents of the prepared vaccines are high, and all not less than 10<8.5>ELD50/ml, so that the duck hepatitis bivalent live vaccines have the characteristics of good safety and excellent immunogenicity, and break through limitations of DHV-3 type virus strains which only can be proliferated in duck embryos; 2) virus solutions cultured by the SPF chicken embryos lower risks of exogenous pathogenic microbial contamination, improve labor efficiency and reduce production costs, thereby being suitable for mass production; 3) the bivalent live vaccines disclosed by the invention can prevent DHV-1 type and DHV-3 type virulent viruses attacking on ducklings; 4) the duck hepatitis bivalent live vaccines are simple in production process, easy in quality control and small in batch difference, thereby providing a guarantee for producing safe and qualified vaccines.

The invention relates to a testing or inspection method containing enzymes or microbes, in particular to a fluorescence detection kit for simultaneous detection of three kinds of breast cancer tumor markers and a detection method thereof. The kit for the simultaneous detection of three kinds of breast cancer tumor markers is characterized in that the three kinds of breast cancer tumor markers arerespectively CEA, CA153 and CA125; the kit comprises ten kinds of solutions and respectively G1 solution, G2 solution, G3 solution, Q1 solution, Q2 solution and Q3 solution. Compared with the prior art, the method provided by the invention has the advantages that the simultaneous detection on three kinds of breast cancer markers can be realized through switching different maximum emitting wave lengths.

The invention discloses a porcine circovirus, porcine pseudorabies virus and mycoplasma triple inactivated vaccine which comprises an antigen and a vaccine adjuvant, the antigen is composed of a porcine circovirus type 2 antigen, a porcine pseudorabies virus antigen and a mycoplasma antigen, the porcine circovirus type 2 antigen is a purified, concentrated and inactivated porcine circovirus type 2 protein antigen solution, and the content of Cap protein is more than or equal to 160 [mu]g/ml; the porcine pseudorabies virus antigen is a purified, concentrated and inactivated porcine pseudorabies virus protein antigen solution, and the content of the Cap protein is more than or equal to 160 [mu]g/ml; the mycoplasma antigen is an inactivated mycoplasma protein antigen solution, and the content of the Cap protein is more than or equal to 160 [mu]g/ml; and the vaccine adjuvant is composed of a water-based high-molecular polymer adjuvant and a composite polysaccharide immunopotentiator. Foreign protein is removed through clarification filtration and ultrafiltration concentration, and the side reaction probability of the vaccine is greatly reduced; and three-proofing can be achieved through one needle, so that the number of immunization times and stress are reduced. The method is economical and practical, the immunization procedure is simplified, and the epidemic prevention cost is reduced.

The invention provides a method for culturing an avian reovirus. According to the method, because a vero cell which has excellent performance and is conducive to the proliferation of the avian reovirus is used as a host cell to be used for proliferating the avian reovirus, an optimized process for culturing the avian reovirus can be determined. A bioreactor is adopted to culture and replicate the avian reovirus, and due to the adhesion of the cell to a carrier, the suspension culture of the cell can be realized, the number of vero cells in unit volume can be increased, and further, the yield of the avian reovirus can be improved. Meanwhile, because the avian reovirus culturing process can be monitored timely and controlled quantitatively, the stability of the production process can be guaranteed. Moreover, because the content of avian viral arthritis viruses in an avian viral arthritis virus liquor cultured by adopting the method is high, the content of antigens in the prepared inactivated vaccine is high, and the effect of the inactivated vaccine is good. Furthermore, due to the way of proliferating the avian reovirus by using the bioreactor through the microcarrier, the cost can be lowered, the labor can be saved, and the stability of the product can be improved.