42results about How to "High antigen content" patented technology

The invention provides a poliomyelitis inactivated vaccine and a production method thereof. The poliomyelitis inactivated vaccine is produced by applying a basket-type bioreactor and adopting Vero cells as a matrix. The production method comprises the following specific steps of adding an M199 culture medium into the basket type reactor, inoculating the Vero cells for culture, and after a compact monolayer is grown, washing and changing with Earle's balance salt solution; after the washing and the changing are finished, injecting M199 culture medium solution, inoculating poliomyelitis virus with virus inoculation MOI being 0.01-0.3, further culturing with the temperature being 33+ / -0.5 DEG C, dissolving oxygen by 40-80%, controlling a pH value of a system at the virus culture stage to 7.4-7.6; and after inoculation, culturing for 42-72 hours, thereby obtaining virus solution. The poliomyelitis inactivated vaccine and the production method provided by the invention have the advantages that the proper culture medium is selected and the conditions of proper culture temperature, pH value, inoculating proportion and dissolved oxygen and the like are controlled, so that the virus titer is high, the antigen content is high, the height for differences between batches can be controlled and the mass is uniform.

The invention provides a preparation method for human diploid cell rabies vaccine virus solution, and relates to the field of biotechnology. The preparation method comprises the step of inoculating rabies vaccine fixed virus PM-1503-3M strain in MRC-5 cell to generate the human diploid cell rabies vaccine virus solution. The method disclosed by the invention is simple in process and cost-saving; and the prepared virus solution is high in antigen content, high in virus valence and titer, suitable for large-scale industrialized production, and capable of meeting the needs of domestic and foreign markets on human diploid cell rabies vaccine.

The invention discloses a method for preparing a porcine circovirus 2-type inactivated vaccine, and belongs to the technical field of organisms. The method comprises the following steps of: a, cultivation of cells for seeding; b, virus inoculation and cultivation; c, virus liquid harvesting; d, virus liquid inactivation; and e, vaccine preparation. By adopting the method, the seeding cells are screened; the matching property of the cells and the virus is enhanced; the multiplication titer and the harvesting yield of the virus are improved by adopting a torrent perfusion-type bioreactor culture system; the immune effect is improved by improvement of an emulsifying process; the entire production process is not involved with other problems of biosafety and public health; and the method is suitable for mass production.

The invention provides a newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine and a preparation method of the bivalent inactivated vaccine, which relate to the field of biological engineering. The bivalent inactivated vaccine comprises a newcastle disease virus LaSota strain and an avian influenza virus A / chicken / HeNan / 03 / 2009 / (H9N2) strain. The preparation method of the vaccine comprises the steps of preparing, concentrating and deactivating virus liquid, performing water phase preparation and oil phase preparation, and emulsify, thereby obtaining the bivalent inactivated vaccine. The newcastle disease and H9N2 subtype avian influenza bivalent inactivated vaccine has good immunogenicity, creates a high antibody level, and can withstand not only the attack of homologous viruses, but also the attack of JX02 and SH01 of H9N2 subtype avian influenza viruses, which are prevalent since 2011; the bivalent inactivated vaccine can create high level antibodies and has a long persistent period; and the preparation method of the bivalent inactivated vaccine is simple and safe.

The present invention provides a double inactivated vaccine of bovine infectious rhinotracheitis virus and mycoplasma bovis, a preparation method thereof, and suspended MDBK cells used therein. The invention provides suspension MDBK cells, and on this basis, establishes a method for suspending IBRV antigen virus liquid. The IBRV antigen virus liquid obtained by the method has the characteristics of high antigen content, large antigen batch, stable batch, etc. The side reactions caused by the bovine serum added to the culture medium can save the economical cost while producing a safer and more efficient vaccine. At the same time, the IBRV and MB double inactivated vaccine of the present invention can prevent two diseases with one injection, simplify the immunization procedure, reduce the number of immunizations of animals, reduce the stress of animals, save labor, and further reduce the cost of animal breeding.

The invention provides a porcine epidemic diarrhea virus variant strain 2a, 2b bivalent inactivated vaccine and a preparation method thereof. The bivalent inactivated vaccine includes two currently popular porcine epidemic diarrhea variant strains 2a and 2b subtypes, which can prevent diarrhea caused by these two different variant strains. One injection is dual-purpose, safe and effective, and reduces animal immunity. And stress times, piglets can be protected by immunizing sows. The preparation method comprises: fully suspending and culturing porcine epidemic diarrhea virus variant strains 2a and 2b without serum, mixing the inactivated porcine epidemic diarrhea virus variant strain 2a and 2b virus liquids in proportion, adding adjuvant and fully emulsifying to obtain the product. The invention adopts the full-suspension serum-free ST cell culture process to prepare the virus liquid with high antigen content, easy scale-up culture, large batch size and small batch-to-batch difference. The process reduces the risk of pollution and low cost, and provides an effective means for the prevention and control of PEDV.