A kind of tumor-specific antigen and its application

Abstract

The invention discloses a tumor specific antigen and application thereof. The amino acid sequence of the tumor specific antigen TOP-1-40 is shown as SEQ ID NO:1. A protein dimensional electrophoresis method is used for being combined with a cancer serological immunoblotting and antibody capture enzyme-linked immunoassay method for separating and obtaining the tumor specific antigen from cancer cell strain cells and cancer tissues. Through the specific polyclonal antibody for resisting the antigen, the antigen is determined to be a protein fragment, with the molecular weight being about 40kd, of DNA topoisomerase I by using the methods of a recombinant protein method, an immunoblotting method, a fluorescent-immunohistochemistry method, an antibody capture enzyme-linked immunoassay method and the like; the antigen is named as TOP-1-40. The antigen content is generally increased in common cancer tissues; the antigen cannot be detected in corresponding normal tissues, or the content is very low. The self antibody concentration of the antigen in the serum is detected by the antibody capture enzyme-linked immunoassay method; the antigen can be used for the early stage screening of common cancer. The detection method has 95 to 100 percent of specificity and 61 to 66 percent of sensibility. The tumor specific antigen has good prospects in clinic application.

Description

technical field

[0001] The invention belongs to the field of biomedicine, and in particular relates to a tumor-specific antigen and its application. Background technique

[0002] Cancer is one of the major diseases that endanger human health. According to domestic and foreign investigation reports, the death cases caused by cancer account for the first place in the number of deaths due to diseases. The main reason is that cancer is not easy to be detected early, and most of the clinically diagnosed cases have reached the middle and late stages of the disease. The existing clinical methods for treating cancer have limited curative effect on advanced cancer patients, but have remarkable effect on early-stage cancer patients. Therefore, one of the best ways to treat cancer is to be able to detect it early.

[0003] Medical imaging such as CT, B-ultrasound, X-ray and pathological biopsy cannot be discovered until the cancer cells have developed to a certain extent. Detection...

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