Porcine circovirus type 2 subunit vaccine, and preparation method and application thereof

A subunit vaccine, porcine circovirus technology, applied in botanical equipment and methods, biochemical equipment and methods, applications, etc., can solve problems such as unoptimized production process conditions, loss of protein immunogenicity, and difficulty in cultivation , to achieve the effect of improving viral protein yield and quality, enhancing specific antibody response, and improving expression efficiency
CN102925486AActive Publication Date: 2013-02-13WUHAN CHOPPER BIOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN CHOPPER BIOLOGY
Publication Date
2013-02-13

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Abstract

The invention relates to a porcine circovirus type 2 subunit vaccine, and a preparation method and an application thereof. The recombinant baculovirus contains double promoters (a polyhedrin protein promoter and a P10 promoter), and can express double copies of Cap protein coding genes, such that protein expression efficiency is substantially improved. Also, Cap protein expressed by an inserted exogenous gene does not contain excess sequences, such that virus-like particles (VLPs) can be effectively formed, expressed protein immunogenicity is improved, and the content of produced antigen is high. According to the porcine circovirus type 2 subunit vaccine provided by the invention, protein yield and quality of porcine circovirus type 2 subunit vaccine are substantially improved, and prepared vaccine compositions have the advantages of stable and long-lasting immune effect, high safety, and the like.
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Description

technical field

[0001] The invention relates to the technical field of veterinary biological products, in particular to a porcine circovirus type 2 subunit vaccine and a preparation method and application thereof. Background technique

[0002] Porcine circovirus type 2 (PCV2) is the main causative agent of multisystemic wasting syndrome (PMWS) in weaned piglets, which is characterized by immunosuppression and multisystem failure in weaned piglets. , anemia, dyspnea, diarrhea, weakness, slow weight gain, swollen lymph nodes and other clinical symptoms. Since the outbreak of PMWS in Canadian pig herds in 1991, PMWS has caused great economic losses to the swine industry worldwide. PCV2 can not only cause the failure of multiple systems in weaned piglets, but also damage the immune function of infected pigs, resulting in a decrease in the body's resistance, causing secondary infections and aggravating the disease. In addition, PCV2 has been associated with conditions such as r...

Claims

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