Optimized SARS coronary virus spike protein gene

A coronavirus and protein technology, applied in the fields of genetic engineering, vaccinology, and virology, can solve problems such as the absence of a SARS vaccine

Inactive Publication Date: 2005-01-26
陈克勤 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, no truly effective SARS vaccine has been developed so far

Method used

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Examples

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Embodiment 1

[0046] Synthesis of optimized S protein coding sequence

[0047] In this embodiment, according to the published amino acid sequence SEQ ID NO: 2 of SARS coronavirus S protein, and the preferred codons of mammals, the coding sequence is optimized according to the following principles:

[0048] (a) There is a bias in the use of codons between different species;

[0049] (b) Differences in codon usage correlate with translation speed;

[0050] (c) the context of the codon and the presence of a particular codon at a particular position can significantly affect the rate of translation;

[0051] In particular, some codons that particularly affect the translation speed are replaced, and the sequence shown in SEQ ID NO: 1 is designed.

[0052] Then use a DNA synthesizer (Mclab Company) to obtain oligonucleotide fragments by conventional methods, then anneal each fragment and perform PCR extension to synthesize the DNA sequence of SEQ ID NO:1.

[0053] The optimized synthetic gene s...

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Abstract

The invention discloses an optimized SARS coronary virus spike protein gene, which codes the SARS coronary virus S protein or immunogenicity fragment, and has 77+/-3% homology with SARS coronary virus S protein coding sequence(such as SEQ ID NO: 1). The invention also provides vector, host cell and vaccine composition containing a optimized S protein coding sequence, whose expression efficiency is higher in mammal cell, So can stimulate the immune response in mammal to SARS.

Description

technical field [0001] The present invention relates to virology, genetic engineering and vaccinology, more specifically relates to the optimized SARS coronavirus spike protein gene. This gene can be used for high-efficiency expression and production of spike protein in mammalian, especially human cell environment. The invention also relates to vectors and vaccines containing the gene. Background technique [0002] Atypical pneumonia, referred to as SARS, also known as Severe Acute Respiratory Syndrome (SARS), has spread to more than 30 countries and regions around the world, especially including China, Hong Kong, Singapore, Canada, Vietnam, Taiwan, and the United States. Serious loss of life and property has been caused. Recent epidemiological studies estimate that the mortality rate can be as high as nearly half of patients over the age of 60. [0003] A new SARS coronavirus has been identified as the causative agent of SARS, and the genome of the virus has been sequenc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215C07K14/165C12N7/01C12N15/50C12N15/63C12N15/86
Inventor 陈凌陈克勤
Owner 陈克勤
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