3 results about "Polyhedrin" patented technology

The present invention relates to the treatment of diseases associated with inflammation including solid cancers. More particularly, certain methods relate to the administration (e.g., intravenously) of polyhedrin protein (PODS) crystals for the delivery of one or more therapeutic proteins. This enables targeted release of low doses of the therapeutic protein at sites of the disease.

This invention relates to the field of pharmaceutical technology, and more particularly to fusion proteins of human heavy chain ferritin or its variants with polyhedral or granular proteins and their applications. This invention fuses a ferritin variant with a baculovirus polyhedral protein subunit to obtain particles with a diameter <20 nm, consisting of a polyhedral protein outer layer and a ferritin inner layer. The particles have an outer diameter of 17 nm, an inner lumen of 13 nm, and are positively charged. pH adjustment enables the depolymerization and recombination of the outer and inner layers, allowing the encapsulation of negatively charged insulin molecules with a diameter >8 nm. Proteins fused with polyhedral or granular protein subunits to wild-type or mutant ferritin can self-assemble into bilayer nanoparticles. The inner layer is a cage-like structure with a positive or negatively charged lumen and a size of 8 nm or 13 nm; the outer layer is a polyhedral or granular protein layer, exhibiting resilience, resistant to high temperatures and dehydration, and resistant to digestion by pepsin and trypsin. These nanoparticles can serve as oral carriers for various active molecules.

The invention relates to the technical field of medicines, in particular to a fusion protein of human heavy-chain ferritin or a variant thereof and polyhedrin or granulocyte and application of the fusion protein. A ferritin variant and a baculovirus polyhedrin subunit are fused to obtain a particle which is composed of a polyhedrin outer layer and a ferritin inner layer and has the particle size smaller than 20 nm, the outer diameter of the particle is 17 nm, the inner cavity of the particle is 13 nm, the particle is positively charged, depolymerization and recombination of the outer layer and the inner layer are achieved through pH adjustment, and insulin molecules which are negatively charged and larger than 8 nm can be entrapped. The protein fused by the subunit of the polyhedrosis or granulate protein and the ferritin wild type or mutant type can be self-assembled into a nano-particle with a double-layer structure, and the inner layer of the nano-particle is of a cage type structure with positive electricity or negative electricity in the inner cavity and the size of 8 nm or 13 nm; and the outer layer is a polyhedrin or granular protein layer, has stress resistance, can resist high temperature and dehydration, and can resist digestion of pepsin and trypsin. The nanoparticles can be used as oral carriers of various active molecules.