71 results about "Polyhedrin" patented technology

The invention relates to a 2 type subunit vaccine for a porcine circovirus as well as a preparation method and application thereof. A recombinant bacilliform virus contains double promoters (a polyhedrin promoter and a P10 promoter), a coding gene of a Cap protein with double copying can be expressed, and the expression efficiency of the protein is obviously enhanced; moreover, the Cap protein expressed by an inserted foreign gene does not contain an excess sequence, virus-like particles (VLPs) can be effectively formed, and the immunogenicity of an expressed protein is enhanced; furthermore, a produced antigen has high content; and according to the 2 type subunit vaccine for the porcine circovirus, which is disclosed by the invention, the productivity ratio and the quality of a viral protein of the 2 type subunit vaccine for the porcine circovirus are obviously enhanced, and a prepared vaccine composition has the advantages of stable and persistent immune effect, high safety and the like.

The invention relates to a porcine circovirus type 2 subunit vaccine, and a preparation method and an application thereof. The recombinant baculovirus contains double promoters (a polyhedrin protein promoter and a P10 promoter), and can express double copies of Cap protein coding genes, such that protein expression efficiency is substantially improved. Also, Cap protein expressed by an inserted exogenous gene does not contain excess sequences, such that virus-like particles (VLPs) can be effectively formed, expressed protein immunogenicity is improved, and the content of produced antigen is high. According to the porcine circovirus type 2 subunit vaccine provided by the invention, protein yield and quality of porcine circovirus type 2 subunit vaccine are substantially improved, and prepared vaccine compositions have the advantages of stable and long-lasting immune effect, high safety, and the like.

The invention discloses a direct PCR detection method of Bombyx mori nuclear polyhedrosis pathogeny BmNPV, comprising the following steps of: firstly, treating the blood of infected Bombyx mori by ethanol precipitation to remove a PCR reaction inhibitor; designing the PCR primer of a polyhedrin gene according to the genome analysis of the Bombyx mori nuclear polyhedrosis pathogeny BmNPV; carrying out PCR amplification on the polyhedrin gene, adding an anti-PCR reaction inhibitor BSA (Bull Serum Albumin) to a reaction system and simultaneously carrying out PCR reaction by the primer of a Bombyx mori chondriosome CO I gene to make positive control; respectively getting the blood of normal Bombyx mori and the inflected midguts of the Bombyx mori infected with white muscardine, green muscardine, a bacterial disease and a microsporidia disease and then carrying out PCR reaction specification analysis; and detecting the PCR reaction result by agar gel electrophoresis. The whole detection process has simple operation and can be completed only by 4 hours. The disease can be diagnosed at the early stage of BmNPV infection by adopting the direct PCR detection method to carry out periodical sampling detection so as to adopt a measure in time to prevent the disease from happening.

The invention relates to a method for expressing and purifying fusion protein based on bombyx mori baculovirus polyhedron dissolving characteristic and a high-efficiency fusion expression vector for implementing the fusion protein expression and purification method. The method comprises the following steps of: constructing a bombyx mori polyhedrin gene (Po1h) and a target protein gene into a prokaryotic expression vector and introducing a protease enzyme digestion site between the bombyx mori polyhedrin gene (Po1h) and the target protein gene so as to perform fusion expression in a prokaryotic expression bacterium; due to the solubility characteristics that the polyhedrin is dissolved under the alkaline condition and precipitated under the neutral condition, washing the precipitate, which is obtained after ultrasonic induction of the thallus, by using buffer solutions with different pH values; dissolving the precipitate collected finally by using a buffer solution with high pH value; centrifuging, adjusting the pH value of the collected supernate to be neutral; centrifugally collecting the precipitate which is the fusion protein obtained through separation and purification; performing enzyme digestion on specific protease and performing purification, performing nickel column purification on the polyhedrin tag of the fusion protein to obtain the target protein, so that the fusion protein purification method based on bombyx mori baculovirus polyhedron dissolving characteristic and prokaryotic expression is formed.

A method for constructing a recombinant double-expression silkworm polyhedral baculiform virus which contains the SOD gene comprises that the general RNA of the silkworm is extracted and an MnSOD primer is designed; a single-stranded cDNA is synthesized and an MnSOD gene is synthesized; the MnSOD gene is connected with a p FastBacHTa to transform XL-Blue cells; and the recombinant donor plasmid p FastBacHTa / MnSOD can be extracted; the baculiform virus DNA of the silkworm is taken as the template to synthesize the silkworm polyhedral gene and the gene is cloned to the p FastBac Dual plasmid; the MnSOD gene is inserted under the P10 promoter of the double-expression plasmid to construct the double-expression vector: Bm polyhedrin pFB dual / MnSOD; after the transinfection of the Bm polyhedrin pFB dual / MnSOD, the double-expression virus DNA which contains the MnSOD gene and the polyhedral gene is distilled; the DNA is transduced into the silkworm culture cell of transinfection with the mediation of transinfection reagent lipidosome to obtain the recombinant virus. Virus particles which can feed and infect the silkworm is provided and the object gene MnSOD is expressed by the silkworm and large-scale and industrial production is realized.

Provided is a method of preparing platinum nano particles by using cotton bollworm karyotype polyhedrin. Inherent structural features of the cotton bollworm karyotype polyhedrin are used, a PtC14 solution is added to a purified cotton bollworm karyotype polyhedral body solution, and the precious metal platinum nano particles which are even in distribution and consistent in diameter of the particles are obtained on the surface of the cotton bollworm karyotype polyhedrin through hatch, ultrasonic wave assistance reduction treatment. Due to the fact that a cotton bollworm karyotype polyhedron carrier has natural specific structures of functional group repetitive distribution, the complex preparation technology of a conventional carrier is avoided, and therefore production cost is reduced. The sizes and distribution of the precious metal platinum nano particles are effectively controlled through control of the alkaline hydrolysis degree of the cotton bollworm karyotype polyhedron, and the method of preparing the platinum nano particles by using the cotton bollworm karyotype polyhedrin can be significantly applied to catalytic fuel cells or biosensing or the like.

The invention discloses a dual-host recombination rhabdovirus expression vector and a construction method and application thereof, belonging to the technical field of gene engineering. The dual-host recombination rhabdovirus expression vector is provided with a recognizable Polyhedrin promoter of an insect cell and a recognizable CMV-IE promoter of an animal cell. Dual-host recombination rhabdovirus can be used for preparing a functional protein on the insect cell or can express a target foreign protein on the animal cell and can be used for gene therapy and nonreplication vector vaccine. The construction method of the dual-host recombination rhabdovirus expression vector comprises the following steps of: taking a transfer vector pFas in a Bac-to-Bac expression system as a base; introducing a CMV-IE promoter expression kit on the upstream of the Polyhedrin promoter; constructing the transfer vector; transforming a DH10Bac competent cell; recombining a dual-host Bacmid shuttle vector; and then transfecting insect cells in a logarithmic phase to obtain the dual-host recombination rhabdovirus expression vector.

The invention relates to the technology of antigen protein expression, and particularly relates to a method for preparing a cyprini herpesvirus II antigen coated polyhedrosis based on a baculovirus expression system. According to the method, by designing and recombining bombyx mori nuclear polyhydrosis virus BmNPV-VP3-cyHV-polh, 1-186, 993-1197, 603-783 and 85-186 regional sequences of ORF72, ORF66, ORF81 and ORF82 and the coding sequence of the 1-279 region of a VP3 gene of bombyx mori cytoplasmic polyhedrosis virus structural protein are connected in series to form a fused sequence which is controlled by baculovirus P10 promoter; and the bombyx mori cytoplasmic polyhedrosis protein gene is controlled by a polyhedrin gene promoter of baculovirus. The virus is used for inoculating bombyx mori or bombyx mori culture cells, the recombinant virus expressed cyprini herpesvirus II antigen protein can be coated in bombyx mori cytoplasmic polyhedrosis; the formed polyhedrosis can be purified by simple differential centrifugation; the purified polyhedrosis is cracked under a basic condition, the polyhedrosis protein can be precipitated by centrifuging, and the cyprini herpesvirus II antigen is reserved in supernatant, so that the cyprini herpesvirus II antigen can be quickly and conveniently obtained.

The invention relates to a method for preparing platinum nanowires through helicoverpa armigera nuclear polyhedron extractives. The method mainly includes the steps that after centrifugal purification is carried out on helicoverpa armigera nuclear polyhedrosis virus raw powder, resuspension with ultrapure water is carried out, alkaline hydrolysis liquid is added for processing, chloroform is added for even shocking, supernate is taken for overspeed centrifugation, ultrapure water is added for dissolving sediment spots, and then the polyhedrin extractives are obtained; a K2PtC16 solution is added into the obtained extractives, sufficient and even mixing is carried out, hatching is carried out in an air bath table at the indoor temperature for 20 h-50 h, a NaOH solution is used for adjusting the pH value to range from 7 to 9, and hatching is continuously carried out for 20 h to 50 h; a NaBH4 solution is added for reduction, and the platinum nanowires with net structures are obtained. The simple and environmentally-friendly platinum nanowire synthetic method which is even in structure and shape distribution, adjustable is size and better in stability is provided, and the prepared net-shaped platinum nanowires are even in shape and stable in structure and have the good potential application value on the aspects of fuel cell, biosensing, organocatalysis and the like.

Cypoviruses and baculoviruses are notoriously difficult to eradicate because the virus particles are embedded in micron-sized protein crystals called polyhedra. The remarkable stability of polyhedra means that like bacterial spores these insect viruses remain infectious for years in soil. Although these unique in vivo protein crystals have been extensively characterized since the early 1900s, their atomic organization remains elusive. Here we describe the 2 crystal structure of both recombinant and infectious silkworm cypovirus polyhedra determined using 5-12 micron crystals purified from insect cells. These are the smallest crystals yet used for de novo X-ray protein structure determination. It was found that polyhedra are made of trimers of the viral polyhedrin protein and contain nucleotides. Although the shape of these building blocks is reminiscent of some capsid trimers, polyhedrin has a new fold and has evolved to assemble in vivo into 3-D cubic crystals rather than icosahedral shells. The polyhedrin trimers are extensively cross-linked in polyhedra by non-covalent interactions and pack with an exquisite molecular complementarity similar to that of antigen-antibody complexes. The resulting ultra-stable and sealed crystals shield the virus particles from environmental damage. The structure suggests that polyhedra can serve as the basis for the development of robust and versatile nanoparticles for biotechnological applications such as in cell culture systems, microarrays and biopesticides.

The invention relates to the technical field of gene engineering, in particular to a recombinant vector containing polyhedrosis genes and a method for expressing and purifying proteins. The invention provides a recombinant plasmid pBacPAKph, a recombinant plasmid pBacPAKph-M and a linear virus recon with a connection number of CGMCC No.2768. Linear virus recon BmBacPAKph-M is adopted to infect a host body and express fusion protein of polyhedrosis protein and target protein, the expressed fusion protein is separated and purified, enzyme digestion is carried out on prolease to obtain the target protein. The purified target protein can be prepared into intravenous medicines or used as an object for study on protein functions. A plurality of proteins which are provided by the invention and hard to be expressed and purified but can be easily expressed and purified by protein expression and purification systems, especially membrane proteins have a broad prospect of application.

A method for preparing a precious metal palladium nanorod by means of cotton bollworm baculovirus nucleocapsid mainly includes the steps that cotton bollworm karyotype polyhedron virus raw powder is dispersed through ultra-pure water to obtain grey polyhedrin suspension, alkaline hydrolysis liquid is added into the grey polyhedrin suspension, the mixture stands still for 15-25 min, then the pH value is adjusted to be 7 through acetic acid, chloroform is added, centrifugalization is carried out for 5-10 min at the speed of 5000-6000 r / min, supernatant fluid is sucked, centrifugalization is carried out for 1-2 h at the temperature of 4-10 DEG C and at the speed of 25000-30000 r / min, pigmentation is re-suspeneded through a PBS solution, and a cotton bollworm baculovirus nucleocapsid solution is obtained; a PdCl2 solution is added into the cotton bollworm baculovirus nucleocapsid solution and fully and evenly mixed, incubation is carried out in a shaking mode for 15-25 h at the temperature of 20-30 DEG C and at the speed of 130-170 r / min, and the precious metal palladium nanorod loaded by the cotton bollworm baculovirus nucleocapsid is obtained. The method is simple in process, environmentally friendly and efficient, industrial production is easy to achieve, and the prepared palladium nanorod is stable in appearance and uniform in size.

The invention discloses a method for realizing efficient covering of a foreign protein by using an insect virus polyhedron, wherein the polyhedrin of a bombyx mori cytoplasmic polyhedrosis virus (BmCPV) and a target protein gene are constructed under the PH and P10 promoters on a pFastBac Dual carrier, and a baculovirus capable of expressing the polyhedron and the target protein simultaneously can be obtained through recombination. The method is characterized in that the cytoplasmic polyhedrosis protein gene and the target protein gene are simultaneously constructed under the PH and P10 promoters on the pFastBac TMDual carrier through a baculovirus expression system, respectively, a flexible link peptide is introduced between the target protein and a signal peptide, and the recombinant baculovirus is obtained through recombination with a virus skeleton. Once Sf9 or Sf21 insect cells are infected by the single virtus, the desired covered protein can be directly generated in cells; the recombinant virtus also can be amplified.

A genetically engineered virus of Chinese ballworm is obtained by double recombination of wild virus. Its genome is deficient is egt gene. At the position deficient in egt gene, the insect-specific neurotoxic AaIT gene controlled by the promoters of Chinese ballworm virus' alkaline DNA binding protein gene (p6.9) and polyhedrin protein gene (ph) and the beta-galactosidase (LacZ) gene controlled by the promoter of heat shock protein gene (hsp70) are inserted. Its advantages are higher insecticiding speed and better application effect.

The invention provides a mink parvovirus virus-like particle which is characterized in that VP2 gene of mink parvovirus is optimized according to insect cell optimal codons, the 5' end of the optimized VP2 gene is directly connected with the nucleotide sequence of polyhedron of a coding part and then is cloned into a transfer vector, and the mink parvovirus virus-like particle can be produced through a baculovirus / insect cell expression system. The mink parvovirus virus-like particle can be produced through the expression system in a safe, efficient and large-scale manner, and the obtained virus-like particle is high in titer and good in immunogenicity; both muscle immunization and oral immunization can induce a mink body to produce high-level specific antibody which can resist attack of virulent virus and well protect a mink, and a foundation is laid for the preparation of mink viral enteritis vaccine.

The invention relates to a method for implementing gene engineering genetic improvement on a gp64-free baculovirus of a genome by using a Bac-to-Bac system. The method comprises construction of a recombinant virus, amplification of a recombinant virus polyhedrosis and biological assay of the recombinant virus; and according to the construction strategy, a gp64 gene controlled by an autographa californica multiplenucleopolyhedrovirus (AcMNPV) polyhedrosis virus gene promoter and a cyst membrane glycoprotein gene promoter of an orgyia pseudotsugata multiplenucleopolyhedrovirus (OpMNPV) polyhedrosis virus together and a helicoverpa armigera single nucleopolyhedrovirus (HearNPV) polyhedrin gene controlled by an AcMNPV polyhedrin gene promoter and a HearNPV polyhedrin gene promoter together are inserted into a polyhedrin gene site of HearNPV bacmid to construct a recombinant HearNPV bacmid for co-expressing two cyst membrane glycoproteins, namely GP64 and F proteins. The recombinant virus has better insecticidal activity and higher biological safety than a wild virus.

The invention provides a method for preparing a platinum-copper alloy nanowire by heliothis armigera nuclear polyhedrin. The method mainly comprises the following steps: preparing heliothis armigera polyhedrin freeze-dried powder into a polyhedrin solution with the concentration of 0.0001-0.01mg / mL; preparing a K2PtC16 solution and a CuCl2 solution into a precursor mixed solution with the molar ratio of Pt atoms to Cu atoms being (1-3) to (1-3); preparing the polyhedrin solution and the precursor mixed solution into the mixed solution at the volume ratio of (3-6) to 1; and introducing ultra-pure nitrogen into the mixed solution for 5-30 minutes, adding sodium borohydride to the solution for reduction at a room temperature and changing the solution into black from faint yellow to prepare the network platinum-copper alloy nanowire. According to the method, the preparation time is especially short; the technology is simple; low-cost industrial production is easy to implement; the platinum-copper alloy nanowire has very high catalytic activity on electrochemical oxidation of ethanol, has good carbon monoxide poisoning resistance and good stability, and can be used as a fuel cell catalyst.

The invention relates to a qualitative detection method of a tea geometrid nuclear polyhedrosis virus, which belongs to the technical field of the detection of biological preparations and is characterized by comprising the following steps of: firstly, preparing a polyhedrin monoclonal antibody of the tea geometrid nuclear polyhedrosis virus, then coating a sample to be detected and detecting by using an indirect Elisa method. The invention breaks through a traditional biological assay technology by means of an immunological means and solves the problem of accurate qualification of the tea geometrid nuclear polyhedrosis virus. Compared with a traditional biological assay method, the virulence index detection of the method is finer, the virulence evaluation period is greatly shortened, the method can be widely used for estimating the quality of a virus preparation and specification of the production and the application of a tea geometrid virus.

An insect control sheet containing a Cry polyhedron prepared by fixing an insecticidal protein (a Cry toxin) produced by Bacillus thuringiensis to a polyhedron of polyhedrin protein is provided. The insect control sheet contains the Cry polyhedron and is used by floating on water. The insect control sheet is floatable on water, and includes a pure matrix layer 20 and a toxin-containing matrix layer 30 containing the Cry polyhedron 51 which are layered on the underside of a sheet-shaped first sheet substrate 10. The pure matrix layer 20 is composed of a degradable or water-soluble second material and the toxin-containing matrix layer 30 is composed of a degradable or water-soluble third material and the Cry polyhedron. The toxin-containing matrix layer sustainably releases the Cry polyhedron to the water on which the insect control sheet is floated.

To provide a useful objective protein coated with a protective protein, a protein complex that is produced in cells and has a structure that the objective protein is occluded with the viral occlusion body protein. A protein complex having the structure that the objective protein is occluded with the viral occlusion body protein, produced in insect cells or plant cells, preferably produced by incorporation of the objective protein in the crystalline form during crystallization of polyhedron derived from polyhedrosis virus, preferably a protein complex in which the polyhedrin contributes to improvement of stability, or protection, or improvement of preservability, or combination thereof, of the objective protein.

A process for increasing the expression to exogenous gene in the insect baculovirus system by insect hormones features that the molting hormone (MH), the juvenile hormone (JH) and their mixture are used to increase the expression efficiency of exogenous gene and polyhedron protein gene in the insect baculovirus system and the copying efficiency of insect baculovirus in insect cells.

The invention relates to a recombinant virus and method for producing L-asparaginase II by using the virus, which belong to the technical field of bioscience. The invention has the purpose of providing the recombinant virus. The virus comprises a polyhedrin gene and an L-asparaginase II gene. Therefore, the recombinant virus is transferred to a silkworm to produce the L-asparaginase II connected with the polyhedron by a covalent bond; and then the L-asparaginase II is separated and purified by a polyhedral body; the recombinant virus has the characteristic of high expression level in the silkworm body; and the L-asparaginase II separated and purified by the polyhedral body has the characteristic of high purity.

An insect control sheet containing a Cry polyhedron prepared by fixing an insecticidal protein (a Cry toxin) produced by Bacillus thuringiensis to a polyhedron of polyhedrin protein is provided. The insect control sheet contains the Cry polyhedron and is used by floating on water. The insect control sheet is floatable on water, and includes a pure matrix layer 20 and a toxin-containing matrix layer 30 containing the Cry polyhedron 51 which are layered on the underside of a sheet-shaped first sheet substrate 10. The pure matrix layer 20 is composed of a degradable or water-soluble second material and the toxin-containing matrix layer 30 is composed of a degradable or water-soluble third material and the Cry polyhedron. The toxin-containing matrix layer sustainably releases the Cry polyhedron to the water on which the insect control sheet is floated.

The invention relates to a construction method of a protein microcrystal embedded CyHV-2 (cyprinid herpesvirus II)-type-GCRV (grass carp reovirus) subunit vaccine based on a yeast expression vector. An AOX1 promoter is used for controlling a silkworm cypovirus protein gene; a TEF1 promoter is used for controlling VP6 and VP7 from GCRV and CyHV-2 ORF72, ORF66, ORF81, ORF82 and an antigenic epitopefusion sequence VP6-VP7-ORF72-ORF66-ORF81-ORF82-VP3 obtained after codon optimization of cypovirus VP3. The vector transforms yeast, inducible expression is performed through methanol, expressed poly-antigen epitope fusion protein is embedded in recombinant cypovirus protein under the guide of cypovirus VP3, a protein microcrystal of embedded CyHV-2-GCRV is formed, the protein microcrystal is split by a sodium carbonate-sodium bicarbonate solution, the pH is regulated to 7.12 with a hydrochloric acid solution, and a supernatant obtained after centrifugation is CyHV-2-GCRV joint subunit vaccineantigen.

The invention relates to the technical field of microbes, in particular relating to a genetically engineered virus I (SpltMNPV-Delta egt-egfp) capable of preventing and controlling farming and forestry pests such as cotton leafworm. The virus is preserved in the China Center for Type Culture Collection (CCTCC) with the collection number of CCTCC NO: V201027. In the invention, a genetic engineering method is utilized to delete and recombine the wild virus so as to obtain the cotton leafworm genetically engineered virus. The genome of the virus loses an egt gene, namely an ecdysteroid uridine diphosphate glucosyltransferase gene; and a marker gene, namely an enhanced green fluorescent protein (egfp) gene is inserted to the locus losing the egt gene, wherein the marker gene is controlled by the wild virus polyhedrin (ph) promoter. Compared with the wild virus, the genetically engineered virus has the advantages of higher pesticidal speed, better field application effect and the like.

The invention discloses a construction method and application of an antheraea pernyi multinucleocapsid nucleopolyhedrovirus shuttle vector, and belongs to the field of a biological technology. A bacterium artificial chromosome cloning vector is used for constructing a transfer vector comprising a DNA fragment containing a lacZ alpha report gene and a bacterial transposon Tn7 transposition target point sequence, a DNA fragment containing an AnpeNPV polyhedrin gene promoter sequence and an upstream gene partial sequence, and a DNA fragment containing a polyhedrin gene 3' terminal partial codingregion sequence and a downstream gene partial sequence, and linearized transfer vector plasmid DNA and linearized AnpeNPV genomic DNA are subjected to cotransfection with Tn-High Five cells, so that recombinant AnpeNPV is obtained. The recombinat AnpeNPV genomic DNA is transformed to escherichia coli, so that an AnpeNPV shuttle vector AnpeNPV-bacmid is obtained. The shuttle vector can be reproduced in the manner of plasmid DNA in the escherichia coli, can also be reproduced in the virus DNA manner in Tn-High Five insect cells and antheraea pernyi, and has infectivity.

The invention discloses polyhedrin coated phytase. A cytoplasmic polyhedrin gene and a phytase gene are respectively constructed under PH and p10 promoters of a pFastBac Dual carrier simultaneously by utilizing a baculovirus expression system; an SP guide gene is introduced in the front of the phytase gene; the SP guide gene is recombined with a virus skeleton; and therefore, recombinant baculovirus is obtained. Required polyhedrin coated phytase particles can be directly generated in cells by using the single virus to infect Sf9 or Sf21 insect cells; furthermore, the recombinant virus can also be amplified; the polyhedrin coated phytase particles are 0.1-1 mu m in particle size; and the polyhedrin coated phytase particles have the characteristics of being acid-resistant, high-temperature-resistant, durable, slow to release and the like.

The invention relates to an animal genetic engineering interferon compound preparation, the production method and the clinical application. The animal genetic engineering interferon compound preparation is prepared by extracting the animal peripheral blood or spleen lymphocytes, culturing, inducing by in vitro inductor, extracting the cell total RNA by Trizol, designing the specific primer, cloning the interferon gene by RT-PCR technology and connecting to the pGEM-T carrier by T-A strategy; the primer is designed again, the both ends are added with different restriction endonucleases digestion sequences, initiation codon ATG and termination codon TAA, leading peptide sequences are removed, the PCR amplification is carried out by taking the recombination T carrier as the template, so as to obtain the expression fragment of the animal interferon gene; the expression fragment is carried out with the rare codon mutation, and gel recovery target fragment is connected on the carrier of pFastBacDual with the same double restriction endonucleases digestion after double restriction endonucleases digestion; the interferon-Alpha of an animal is cloned to multiple cloning sites under the control of Polyhedrin promoter, the interferon-Gamma of the same species animal is cloned to multiple cloning sites under the control of p10 promoter; the constructed carrier of pFastBacDual plus interferon Alpha plus interferon Gamma are transfected to DH10BacE.coli to carry out recombination; the constructed recombinant bacmid is extracted and purified after the blue white screening and the PCR identification, and the transfection of SF9 insect cell is carried out by a liposome method; the expression product is carried out with identification and purification; the animal genetic engineering interferon compound preparation is carried out with anti-virus activity detection and the evaluation of the clinical application effects.