6 results about "Polyhedrin" patented technology

The invention discloses a high-efficiency expression and purification method of 5 '-3' exonuclease, which comprises the following steps: based on a Bac-to-Bac system, adding a tag and a TEV restriction enzyme cutting site to optimized 5 '-3' exonuclease Xrn1, then connecting the 5 '-3' exonuclease Xrn1 to a multiple cloning site behind a Polyhedrin promoter of a pFastBac expression vector to prepare expression baculovirus plasmids, and carrying out purification on the expression baculovirus plasmids to obtain the 5 '-3' exonuclease. Then transfecting the recombinant baculovirus particles into insect cells to generate recombinant baculovirus particles; finally, the 5 '-3' exonuclease Xrn1 is obtained through nickel column affinity chromatography, Heparin affinity chromatography and gel filtration chromatography purification, the invention provides a more economical and practical technical route for synthesizing the 5 '-3' exonuclease Xrn1, and a technical foundation is laid for large-scale production of the 5 '-3' exonuclease.

The present invention relates to the treatment of diseases associated with inflammation including solid cancers. More particularly, certain methods relate to the administration (e.g., intravenously) of polyhedrin protein (PODS) crystals for the delivery of one or more therapeutic proteins. This enables targeted release of low doses of the therapeutic protein at sites of the disease.

This invention relates to the field of pharmaceutical technology, and more particularly to fusion proteins of human heavy chain ferritin or its variants with polyhedral or granular proteins and their applications. This invention fuses a ferritin variant with a baculovirus polyhedral protein subunit to obtain particles with a diameter <20 nm, consisting of a polyhedral protein outer layer and a ferritin inner layer. The particles have an outer diameter of 17 nm, an inner lumen of 13 nm, and are positively charged. pH adjustment enables the depolymerization and recombination of the outer and inner layers, allowing the encapsulation of negatively charged insulin molecules with a diameter >8 nm. Proteins fused with polyhedral or granular protein subunits to wild-type or mutant ferritin can self-assemble into bilayer nanoparticles. The inner layer is a cage-like structure with a positive or negatively charged lumen and a size of 8 nm or 13 nm; the outer layer is a polyhedral or granular protein layer, exhibiting resilience, resistant to high temperatures and dehydration, and resistant to digestion by pepsin and trypsin. These nanoparticles can serve as oral carriers for various active molecules.

The present invention relates to self-assembling proteins that can be used to deliver cargo proteins to phagocytes, and the subsequent delivery and utilization of these self-assembling proteins by phagocytes at disease sites. In particular, the present invention relates to phagocytes comprising polyhedrin crystals, wherein the crystals themselves encapsulate therapeutic and / or diagnostic proteins. The present invention also provides therapies using these cells.

The invention relates to the technical field of medicines, in particular to a fusion protein of human heavy-chain ferritin or a variant thereof and polyhedrin or granulocyte and application of the fusion protein. A ferritin variant and a baculovirus polyhedrin subunit are fused to obtain a particle which is composed of a polyhedrin outer layer and a ferritin inner layer and has the particle size smaller than 20 nm, the outer diameter of the particle is 17 nm, the inner cavity of the particle is 13 nm, the particle is positively charged, depolymerization and recombination of the outer layer and the inner layer are achieved through pH adjustment, and insulin molecules which are negatively charged and larger than 8 nm can be entrapped. The protein fused by the subunit of the polyhedrosis or granulate protein and the ferritin wild type or mutant type can be self-assembled into a nano-particle with a double-layer structure, and the inner layer of the nano-particle is of a cage type structure with positive electricity or negative electricity in the inner cavity and the size of 8 nm or 13 nm; and the outer layer is a polyhedrin or granular protein layer, has stress resistance, can resist high temperature and dehydration, and can resist digestion of pepsin and trypsin. The nanoparticles can be used as oral carriers of various active molecules.

The invention discloses a CPVs insecticide based on a bombyx mori cytoplasmic polyhedrosis virus as well as preparation and application thereof. The preparation method comprises the following steps: carrying out enzyme digestion linearization on recombinant plasmids pT-S1, pT-S2, pT-S3, pT-S4, pT-S5, pT-S6, pT-S7, pT-S8 and pT-S9 with full-length cDNA (complementary deoxyribonucleic acid) of S1 to S9 fragments of the BmCPV and a plasmid pT-S10-Bt with a DNA sequence fused by a Bt gene and polyhedrosis protein ORF on an S10 fragment of the BmCPV; the method comprises the following steps of: obtaining linearized plasmids of pT-S1 to pT-S9 and pT-S10-Bt, then transfecting to obtain a recombinant virus BmCPV-Bt, and then preparing the insecticide. When the BmCPV-Bt is ingested by insects, the polyhedrosis-Bt fusion protein is dissolved in alkaline digestive tracts of the insects, and active peptides with toxicity are released to cause rapid death of the insects. According to the technical scheme disclosed by the invention, a novel, green and environment-friendly biological insecticide can be provided for prevention and control of agricultural and forestry pests.