The invention relates to a macropore carrier 'synchronization method' covalent crosslinking-immobilized papain polymer and a method. In the papain polymer, a papain is embedded in the pore canal of a carrier the aperture of which is more than 0.5mu m; an inorganic macroporous material or an organic macroporous material the surface of which is provided with hydroxyl is taken as an immobilized enzyme carrier; synchronous complementation of the formation of the crosslinking papain polymer and the covalent connection of the crosslinking papain polymer and the macroporous carrier is finally realized through amino-group modification, enzyme adsorption, enzyme precipitation and synchronous crosslinking on the surface of the carrier; the enzyme carrying amout of the immobilized enzyme is higher than that of the immobilized enzyme of a common carrier; the enzyme leakage caused by applications of the macroporous carrier is avoided by adopting a covalent fixed method; the carrier shape can be adjusted according to the actual requirements; and the optimum catalysis temperature, pH value, solvent and heat stability are obviously improved. The papain polymer provided by the invention can be applied to other proteases, lipase, amylase, glucose isomerase, penicillin, acylase, pectinase, oxidase, L-asparaginase, aspartase and peroxidase and the like.