88 results about "Synthetic Genes" patented technology

The invention relates to a method for cultivating insect-resistant transgenic Xiaoblack poplar, in particular to a method for cultivating insect-resistant transgenic Xiaoblack poplar. The invention aims to solve the problem of low insect resistance of the populus similis. The cultivation method comprises the following steps: 1, synthesizing a Cry1Ac gene, wherein the nucleotide sequence of the gene is shown as Seq ID No: 1 in a sequence table; 2, a plant expression vector pBI121-Cry1Ac expressed by the Cry1Ac gene is constructed, and the plant expression vector pBI121-Cry1Ac is constructed; 3, preparing an infection solution; 4, cultivating the Cry1Ac-transgenic small black poplar by using an agrobacterium-mediated leaf disc transformation method, the Cry1Ac-transgenic poplar is created, the average concentration of leaf Cry1Ac protein is more than 200ng / g, the insect resistance of the transgenic poplar can be improved, all fall webworms die after being fed for 5 days, and the corrected death rate reaches 100%. The method is applied to the field of molecular breeding.

The invention discloses an application of an overexpressed bellseed AlbHLH175 gene in increasing tropine alkaloid and precursor content thereof, by creating an AlbHLH175 overexpressed transgenic hairy root strain and analyzing expression quantities of AlbHLH175 and TAs biosynthetic genes in the transgenic strain, results show that the AlbHLH175 can positively regulate expression of seven biosynthetic genes, namely AlODC, AlPMT, AlMPO, AlPYKS, AlCYP82M3, AlPPAR and AlHDH, and the expression quantity of the AlbHLH175 and TAs biosynthetic genes in the transgenic strain is analyzed. Therefore, accumulation of putrescine, N-methyl putrescine, tropinone, tropine, conpin, scopolamine, anisodamine and scopolamine is promoted, and an excellent gene resource is provided for creating a stable and high-yield TAs new germplasm of the bellflower through biological breeding and molecular design.

The invention provides an application of a bacillus subtilis strain A45 in preventing and treating plant diseases or an application of the bacillus subtilis strain A45 in preparing a microbial agent for preventing and treating the plant diseases and the prepared microbial agent. The bacillus subtilis A45 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.33289. According to the present invention, the mechanism of the liquid bacterial agent containing the bacillus subtilis A45 to efficiently inhibit the growth of the cucumber target leaf spot Corynespora cassicola is that the bacterial strain is encoded to synthesize the famogen, the purehydrazide acid, the subtilosin A and the surfactin synthesis gene cluster, the surfactin synthesis gene cluster is different from the synthesis gene of any known organism, and the highest sequence similarity is 82%. The invention further provides the application of the liquid bacterial agent containing the bacillus subtilis A45 to the preparation of the cucumber target leaf spot Corynespora cassicola, and the application of the liquid bacterial agent containing the bacillus subtilis A45 to the preparation of the cucumber target leaf spot Corynespora cassicola. Experiments show that the liquid fungicide containing the bacillus subtilis A45, provided by the invention, can be used for efficiently inhibiting the growth of the cucumber target leaf pathogen corynespora cassicola, and the prevention and treatment effect can be up to 80% when the liquid fungicide is used in a field.

The invention provides a mechanism for regulating and controlling AsA content of kiwi fruits by AcMYB73 and AcMYB108 transcription factors and application of the mechanism. According to the application disclosed by the invention, after GABA is used for treating kiwi fruits, transcriptome sequencing is carried out, and AcMYB73 and AcMYB108 transcription factors which are most likely to regulate and control ascorbic acid-related metabolic genes are screened from numerous transcription factors with most obvious abundance change; experiments such as LUC / REN dual luciferase, yeast single impurity, gel migration and the like prove that both the AcMYB73 and the AcMYB108 can positively regulate and control the ascorbic acid synthesis gene and the circulating gene of the kiwi fruit and reversely regulate and control the ascorbic acid degradation gene of the kiwi fruit at the same time. The invention also successfully constructs overexpression vectors and interference vectors of AcMYB73 and AcMYB108, and successfully realizes regulation and control of the content of ascorbic acid in kiwi fruits.

The application belongs to the field of plant genetic engineering, and particularly relates to a soybean eugenol synthesis gene GmEGS and application thereof. The application provides application of a soybean eugenol synthesis protein GmEGS or a nucleic acid molecule coding the soybean eugenol synthesis protein GmEGS in increasing the content of eugenol in soybean or improving the ability of soybean to resist Phytophthora sojae, the protein GmEGS includes a protein GmEGS1a, a protein GmEGS1c and a protein GmEGS2a, the amino acid sequences of the protein GmEGS1a, the protein GmEGS1c and the protein GmEGS2a are shown in SEQ ID NO. 1-3 in sequence, and the ORF of the nucleic acid includes nucleic acid molecules shown in SEQ ID NO. 4-6. The content of eugenol in soybean can be obviously increased by transforming a soybean plant with the biomaterial of the application, and meanwhile, the disease resistance of soybean to Phytophthora sojae can also be improved. The application provides a good theoretical basis for the molecular mechanism of disease resistance of soybean, and has important significance for molecular design breeding.

The invention discloses a method for increasing the fermentation yield of mernomycin A based on a reinforced chassis strain glycosyl donor. Specifically, in the heterologous expression process of a sirnomycin A biosynthetic gene cluster in a chassis strain Streptomyces albus J1074, a glycosyl donor synthesis module gene and a resistance gene (MaPBP) are introduced at the same time, and on the basis of improving a glycosyl biosynthetic donor, the physiological tolerance of the chassis strain is also enhanced. Compared with a high-yield strain Streptomyces albus LX03 (CGMCC NO. 20985) of which the tolerance is only optimized, the yield of the strain Streptomyces albus LX02: pJQK610 subjected to genetic engineering optimization is improved by 285%, and the yield reaches 114 mg / L. The strain disclosed by the invention has the advantages that the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%, and the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%. The invention provides a high-yield technology for improving heterologous expression of the mernomycin A through collaborative optimization of precursor supply and physiological tolerance, and provides reference for efficient production of the medicine.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

The invention relates to the technical field of biochemistry, and discloses a hydrolase for splitting bacterial peptidoglycan causing chronic inflammatory diseases, the hydrolase is separated from a human intestinal microbe genome, and the hydrolase can hydrolyze a peptidoglycan layer of gram-positive pathogenic bacteria in a targeted manner, so that the bacterial peptidoglycan has the advantages that the bacterial peptidoglycan has a good anti-inflammatory effect, and the bacterial peptidoglycan has a good anti-inflammatory effect. The compound has high efficiency, specificity and low drug resistance and has a synergistic effect with existing antibiotics; the preparation method of the hydrolase comprises the following steps: according to an amino acid sequence, selecting escherichia coli preferred codons, and artificially synthesizing a gene sequence for coding the hydrolase; cloning the synthetic gene into an expression vector to construct a recombinant expression plasmid; transforming the recombinant expression plasmid into escherichia coli, and carrying out induced expression; the hydrolase is obtained through a separation and purification step. According to the hydrolase for splitting the bacterial peptidoglycan causing the chronic inflammatory disease, a peptidoglycan layer of gram-positive bacteria can be efficiently hydrolyzed through the hydrolase, so that the bacteria are rapidly split, and the antibacterial effect is improved.

The present invention provides a recombinant microorganism for simultaneously producing sesquiterpenes and primary metabolites, wherein the primary metabolites include at least one of pantothenic acid, squalene, fatty acids and vitamin B2; the strain contains the mevalonate pathway, the β-farnesene synthesis gene and genes related to the synthesis of primary metabolites; the genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1 and ERG20 genes, and the copy numbers are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1; the β-farnesene synthesis gene includes bFS, and the sequence is as shown in SEQ ID NO.1. The present invention realizes high yield of primary metabolites while producing sesquiterpene compounds.

This application belongs to the field of plant genetic engineering technology and specifically relates to a DpCYP707A2 gene for regulating dioscin synthesis and its applications. The nucleotide sequence of the DpCYP707A2 gene is shown in SEQ ID NO. 1. According to the results of the examples of the present invention, the DpCYP707A2 gene provided by the present invention can regulate the synthesis of dioscin in yam. The DpCYP707A2 gene plays an important role in influencing dioscin synthesis in plants and can help breed new yam varieties with increased dioscin content, thus having broad application value.

This invention relates to the field of biosynthesis technology, and particularly to engineered yeast strains and their application in the total fermentation production of steviol glycosides. This invention utilizes site-directed mutagenesis to mutate wild-type kaurenoic acid 13-hydroxylase, obtaining the G481L / Q159L mutant. This mutant is then applied to the production of steviol or steviol glycosides, constructing a steviol or steviol glycoside synthesis pathway in the *Saccharomyces cerevisiae* host to achieve efficient production of steviol or steviol glycosides. Simultaneously, by enhancing the level of diterpene precursor synthesis genes in chassis cells (MVA pathway, acetyl-CoA, GGPP, transport proteins, UDPG pathway), the yield of steviol glycosides is further increased, reducing costs for industrial production.

The invention discloses a production strain of demethylated ristomycin aglycone as well as a construction method and application of the production strain. The construction method comprises the following steps: synthesizing a gene cluster lacking a glycosylation modification function by adopting a gene editing mode, and then expressing the edited gene cluster in a heterologous or homologous host; or the glycosyl transferase gene in the strain containing the ristomycin biosynthetic gene cluster is knocked out. At present, no report about biosynthesis of the demethylated ristomycin aglycone exists, and the invention provides a biosynthesis method of the demethylated ristomycin aglycone. Meanwhile, the demethylated ristomycin aglycone production strain provided by the invention has good genetic operability, is fast in growth and short in fermentation time, can reach the peak of producing the ristomycin aglycone after being fermented for 2-3 days, and has excellent industrial development potential.

The invention relates to the field of gene engineering, and discloses dsRNA of a bombyx mori glucose regulatory protein 78 gene and a preparation method of the dsRNA, and the preparation method comprises the following steps: a) designing and synthesizing an upstream primer and a downstream primer of a BmGRP78 gene; b) extracting bombyx mori total RNA, performing reverse transcription to obtain first chain cDNA, taking the first chain cDNA as a template, performing PCR amplification to obtain a BmGRP78 gene full-length fragment, purifying, cloning to escherichia coli, and performing sequencing verification; c) designing an upstream primer SEQ ID NO: 3 and a downstream primer SEQ ID NO: 4 of dsRNA according to the nucleotide sequence SEQ ID NO: 1 of the BmGRP78 gene, wherein both the upstream primer and the downstream primer carry a T7 promoter sequence; and d) in-vitro transcription synthesis of dsRNA which can specifically inhibit BmGRP78 gene expression and is used for bombyx mori to optimize silk production and improve disease resistance innovation. Compared with the prior art, the dsRNA has the effect of outstanding gene knock-down efficiency, and experimental results prove that the dsRNA can effectively reduce BmGRP78 gene expression in bombyx mori ovary cells (BmN) and silk glands.

The invention relates to the technical field of bioengineering, in particular to a recombinant halophilic bacterium for producing PHBV by using acetate as a single carbon source and application of the recombinant halophilic bacterium. The expression and / or activity of 2-methyl citrate synthetase and succinate dehydrogenase assembly factor 2 of the recombinant halophilic bacteria provided by the invention are reduced, 3-hydroxyvaleric acid synthetic genes scpA and scpB are overexpressed, an ADP-dependent acetyl coenzyme A synthetase gene acd is overexpressed, and a heterologous monocarboxylic acid transporter gene mctC is expressed. The strain can generate PHBV without adding precursors such as propionic acid and valeric acid, acetic acid or acetate can be used as a sole carbon source to produce PHBV, the yield is close to that when glucose is used as a carbon source, and the problems that the added precursor substances have certain toxicity, so that the growth of thalli is influenced, and the yield is reduced are solved; environmental pollution caused by addition of precursor substances is avoided, and meanwhile, the fermentation production cost of the PHBV is effectively reduced.

Recombinant Sorangium cellulosum for producing de-epoxidized epothilone B by fermentation, insertional inactivation of an epoK gene in an epothilone biosynthetic gene cluster in the recombinant bacteria, and a method for producing de-epoxidized epothilone B using the recombinant bacteria.

The invention discloses streptomyces hygroscopicus ascomycetes subspecies and an obtaining method thereof. Regulating genes fkbN, fkbR1 and fkbR2 on an ascomycin biosynthesis gene cluster of the streptomyces hygroscopicus ascomycetes subspecies ATCC 14891 and a gene fkbS participating in synthesis of an ascomycin precursor ethyl malonyl coenzyme A are subjected to biosynthesis to obtain the streptomyces hygroscopicus ascomycetes subspecies. And carrying out overexpression on a regulatory gene SH03686 of a leucine response regulatory protein family (Lrp) outside the gene cluster, and screening an ascomycin high-yield strain. According to the invention, after combined overexpression of genes fkbS and fkbN and overexpression of a gene fkbR2 are carried out by a strain SN9 and a strain R2-17 in starting bacteria, the transcriptional level of regulatory genes (fkbN, fkbR2) on an ascomycin biosynthesis gene cluster and related genes participating in synthesis of precursors ethyl malonyl coenzyme A (fkbS, fkbE), piperidine acid (fkbL) and DHCHC (fkbO) are improved, and finally, the ascomycin yield is improved.

The invention belongs to the field of plant genetic engineering, and particularly relates to a soybean eugenol synthesis gene GmEGS and application thereof. The invention provides application of a soybean eugenol synthetic protein GmEGS or a nucleic acid molecule encoding the soybean eugenol synthetic protein GmEGS in increasing the content of eugenol in soybeans or improving the resistance of the soybeans to phytophthora sojae. The protein GmEGS comprises a protein GmEGS1a, a protein GmEGS1c and a protein GmEGS2a, and the amino acid sequences of the protein GmEGS1a, the protein GmEGS1c and the protein GmEGS2a are sequentially shown as SEQ ID NO.1-3; the ORF of the nucleic acid comprises nucleic acid molecules as shown in SEQ ID NO. 4-6. When the biological material disclosed by the invention is used for transforming soybean plants, the content of eugenol in soybeans can be obviously increased, and meanwhile, the disease resistance of the soybeans to phytophthora sojae can also be improved. The invention provides a good theoretical basis for a soybean disease-resistant molecular mechanism, and has important significance for molecular design breeding.

The present invention discloses a method for enhancing the transcription level of amino acid synthesis genes to improve the fermentation level of TG enzyme, which is to obtain a mutant strain with high TG enzyme production by over-expressing genes in the synthesis pathways of endogenous proline, histidine, aromatic amino acids, and arginine using its own promoter in Streptomyces pulmonaria IPIO. The present invention enhances the synthesis of proline, histidine, aromatic amino acids, and arginine synthesis genes by enhancing the transcription level of these amino acids, increases the amino acid supply in the TG enzyme synthesis process, and thereby improves the fermentation level of glutamine transaminase. The fermentation yield of glutamine transaminase of the engineered strains WD15, WD18, WD19, and WD22 obtained by the present invention is 22%, 50%, 48%, and 13% higher than that of the control strain at the laboratory shake flask level, respectively.

The invention provides amycolatopsis mediterranei with gene defects as well as a construction method and application of the amycolatopsis mediterranei. The first aspect of the invention provides a construction method of the amycolatopsis mediterranei with the gene defect, and the construction method comprises the step of knocking out a rifamycin biosynthetic gene cluster in a receptor amycolatopsis mediterranei strain U32 (Cas12a) genome and an integrated plasmid pDZLCas12a containing an FnCas12a coding gene to obtain the amycolatopsis mediterranei with the gene defect. According to the invention, a rifamycin biosynthetic gene cluster in a genome of an amycolatopsis mediterranei strain U32 (Cas12a) and an integrated plasmid pDZLCas12a containing an FnCas12a coding gene are knocked out, so that the amycolatopsis mediterranei with gene defects is constructed, and the amycolatopsis mediterranei can be used as a chassis strain for mining identified or unidentified biosynthetic gene clusters in actinomycetes.

This invention provides Bacillus subtilis ( Bacillus subtle The application of strain A45 in the control of plant diseases, its application in the preparation of microbial agents for the control of plant diseases, and the prepared microbial agents. Bacillus subtilis A45 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33289. The liquid microbial agent containing Bacillus subtilis A45 provided by this invention effectively inhibits the cucumber target spot pathogen, *Clostridium multiflorum* (…). Corynespora cassicola The growth mechanism of this strain lies in its encoding a gene cluster for the synthesis of phytosalicylic acid, purchemin, subtilosin A, and surfactant. Among these, the surfactant synthesis gene cluster is unique to this strain, exhibiting a maximum sequence similarity of 82%. Experiments show that the liquid inoculant containing Bacillus subtilis A45 provided by this invention can effectively inhibit the growth of *Clostridium multiflorum*, the pathogen causing target spot disease in cucumbers. Corynespora cashew nut The growth of ) can be controlled by field application with a prevention and control effect of up to 80%.

The invention belongs to the technical field of plant genetic engineering, and discloses a gene for regulating and controlling the endogenous salicylic acid content and disease resistance of rice by using an SA synthetic gene OSD3 participating in rice PAL dependence and application of the gene, and the nucleotide sequence of the OSD3 is SEQ ID NO: 1; the nucleotide sequence of a coding protein region is SEQ ID NO: 2; the amino acid sequence of the coded protein is SEQ ID NO: 3; according to the invention, an OSD3 gene is knocked out through CRISPR / Cas9 under the background of Zhonghua 11, a mutant osd3 of which the SA of a rice leaf is reduced to 20% of that of a wild type is obtained, and an OSD3 overexpression plant is created by constructing an OSD3-pMDC43 overexpression vector.

The primary object of the present invention is to provide a polymer synthase gene which is derived from mangrove soil metagenome, and the method for producing the useful copolymer by using this polymer synthase. Another object of the present invention is to provide an enoyl-CoA hydratase gene which is derived from Streptomyces sp. CFMR 7, and the method for producing the useful copolymer, P(3HB-co-3HHx) with increasing the composition of 3HHx by the expression of this enoyl-CoA hydratase.In order to achieve these objects, an isolated polynucleotide encoding for a polypeptide with polymer synthase activity comprising an amino acid sequence set forth in SEQ ID NO: 1 or 3, or an amino acid sequence set forth in SEQ ID NO: 1 or 3 wherein one or more amino acids is replaced, deleted or added are provided.

This invention belongs to the field of biotechnology, specifically relating to neuropeptides from the root-knot nematode *Euonymus alatus*. nlp12 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Heliotropium indicum* root-knot nematodes, this invention is based on... nlp12 The nucleotide sequence of the gene is SEQ ID NO:1. Gene fragment SEQ ID NO:5 and the corresponding dsRNA were designed and synthesized. The synthesized dsRNA was used to soak second-instar root-knot nematodes. nlp12 Gene expression levels were downregulated, resulting in a mortality rate of 73.2% at 60 hours, and complete mortality of nematodes at 108 hours. Immersion nlp12 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 54%, and the number of female nematodes, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.

The application discloses a rice terpene phytoalexin synthesis gene OsCPS4 and application, relates to the field of plant genetic engineering, and the nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application is a good example that the rice terpene phytoalexin participates in crop insect resistance, has certain reference value for understanding the regulation of the rice terpene phytoalexin on pest resistance, and OsCPS4 the research on the gene provides a good theoretical basis for the molecular mechanism of the rice brown plant hopper resistance gene, and has important significance for molecular design breeding.

The invention provides recombinant DNA molecules comprising novel synthetic Intergenic Sequence Regions for use in plants to reduce the interaction of a first transgene expression cassette on a second transgene cassette when inserted between the first transgene cassette and second transgene cassette. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the novel synthetic Intergenic Sequence Regions. The invention also provides methods to reduce the interaction between transgene expression cassettes using the novel synthetic Intergenic Sequence Regions.

The invention belongs to the technical field of plant genetic engineering and the technical field of soybean breeding, and particularly discloses application of a soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number, the CDS sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.1, the amino acid sequence of the encoded protein is shown as SEQ ID No.2, and the amino acid sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.3. The invention further discloses application of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number. The gene is located in an endoplasmic reticulum, and formation and development of lateral branches are promoted by up-regulating expression quantities of genes such as brassinolide synthesis genes CPD, ROT3 and BR6OX2, auxin response genes IAA, GH3 and SAUR and the like. The invention also discloses three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene, a soybean plant containing the gene editing mutants, and a vector construction method of the soybean plant. The invention further discloses the three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene. Experiments prove that the number of branches of homozygous gene editing mutant phenotypes of the three GmBAS1C genes is remarkably increased. The invention provides a new gene target, a molecular tool and a breeding material for soybean high-branch breeding, and has important agricultural application value.

The invention provides an escherichia coli self-regulation anti-acid module and application thereof. The self-regulation anti-acid module is composed of an acid response promoter, an anti-acid system gene, an ATP synthesis promoting gene and a terminator. Compared with a wild strain, the self-regulation acid-resistant module constructed by the invention can effectively improve the acid stress resistance of recombinant escherichia coli, and the growth ability of the strain containing the self-regulation acid-resistant module under an acidic condition is improved by 72.4%.

The present invention discloses a coding gene sequence and application of porcine C-reactive protein and monoclonal antibody. By optimizing the sequence, the porcine C-reactive protein gene is artificially synthesized, and the synthesized gene is inserted into the pET-28a vector, and then co-transformed into the BL21(DE3) host bacterium together with the chaperone plasmid pTF16 to co-express porcine C-reactive protein and the chaperone protein tig to promote the soluble expression of porcine C-reactive protein; a hybridoma cell is obtained by immunizing with this recombinant protein, and the secreted monoclonal antibody contains the heavy chain variable region named VH and the light chain variable region VL, and is composed of the complementarity-determining regions CDR1, CDR2, CDR3 and the framework region. Experiments prove that the recombinant expressed porcine C-reactive protein has high specificity, and the monoclonal antibody produced by preparation and screening through the recombinant protein can be used in a protein detection kit. The porcine C-reactive protein ELISA detection kit of the present invention is used to detect porcine C-reactive protein, can effectively detect the concentration of porcine C-reactive protein in the serum of pig herds, detect the group inflammation level, and evaluate the health level of pig herds in pig farms.

This invention discloses a genetically engineered bacterium for producing rhamnolipids and its applications, belonging to the field of gene recombination and metabolic engineering technology. The genetically engineered bacterium for producing rhamnolipids disclosed in this invention uses *Yarrowia lipolyticis* ATCC MYA-2613 as the starting strain and heterologously expresses the rhamnolipid synthesis gene. rhlA, rhlB and rhlC, and dTDP-L-rhamnose synthesis gene rmlA, rmlB, rmlC and rmlD This invention discloses a genetically engineered bacterium for producing rhamnolipin, which can efficiently metabolize glucose or glucose-containing cellulose hydrolysates to produce rhamnolipin, achieving a rhamnolipin yield of 42.8 g / L. The genetically engineered bacterium constructed in this invention provides a possibility for the efficient production of rhamnolipin from glucose or glucose-containing cellulose hydrolysates.