63 results about "Synthetic Genes" patented technology

The invention provides a mechanism for regulating and controlling AsA content of kiwi fruits by AcMYB73 and AcMYB108 transcription factors and application of the mechanism. According to the application disclosed by the invention, after GABA is used for treating kiwi fruits, transcriptome sequencing is carried out, and AcMYB73 and AcMYB108 transcription factors which are most likely to regulate and control ascorbic acid-related metabolic genes are screened from numerous transcription factors with most obvious abundance change; experiments such as LUC / REN dual luciferase, yeast single impurity, gel migration and the like prove that both the AcMYB73 and the AcMYB108 can positively regulate and control the ascorbic acid synthesis gene and the circulating gene of the kiwi fruit and reversely regulate and control the ascorbic acid degradation gene of the kiwi fruit at the same time. The invention also successfully constructs overexpression vectors and interference vectors of AcMYB73 and AcMYB108, and successfully realizes regulation and control of the content of ascorbic acid in kiwi fruits.

The application belongs to the field of plant genetic engineering, and particularly relates to a soybean eugenol synthesis gene GmEGS and application thereof. The application provides application of a soybean eugenol synthesis protein GmEGS or a nucleic acid molecule coding the soybean eugenol synthesis protein GmEGS in increasing the content of eugenol in soybean or improving the ability of soybean to resist Phytophthora sojae, the protein GmEGS includes a protein GmEGS1a, a protein GmEGS1c and a protein GmEGS2a, the amino acid sequences of the protein GmEGS1a, the protein GmEGS1c and the protein GmEGS2a are shown in SEQ ID NO. 1-3 in sequence, and the ORF of the nucleic acid includes nucleic acid molecules shown in SEQ ID NO. 4-6. The content of eugenol in soybean can be obviously increased by transforming a soybean plant with the biomaterial of the application, and meanwhile, the disease resistance of soybean to Phytophthora sojae can also be improved. The application provides a good theoretical basis for the molecular mechanism of disease resistance of soybean, and has important significance for molecular design breeding.

The invention discloses a method for increasing the fermentation yield of mernomycin A based on a reinforced chassis strain glycosyl donor. Specifically, in the heterologous expression process of a sirnomycin A biosynthetic gene cluster in a chassis strain Streptomyces albus J1074, a glycosyl donor synthesis module gene and a resistance gene (MaPBP) are introduced at the same time, and on the basis of improving a glycosyl biosynthetic donor, the physiological tolerance of the chassis strain is also enhanced. Compared with a high-yield strain Streptomyces albus LX03 (CGMCC NO. 20985) of which the tolerance is only optimized, the yield of the strain Streptomyces albus LX02: pJQK610 subjected to genetic engineering optimization is improved by 285%, and the yield reaches 114 mg / L. The strain disclosed by the invention has the advantages that the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%, and the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%. The invention provides a high-yield technology for improving heterologous expression of the mernomycin A through collaborative optimization of precursor supply and physiological tolerance, and provides reference for efficient production of the medicine.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The application belongs to the technical field of microbial application, and specifically discloses application of roseofungin Streptomyces roseofunginus in plant disease prevention and treatment. The application finds that after R&M synthesis genes in roseofungin Streptomyces roseofunginus are knocked out, the strain can still synthesize oxazolomycin A. Further finding is that oxazolomycin A shows good inhibitory effect on pepper Phytophthora capsici and Phytophthora sansomei, wherein the EC 50 value of pepper Phytophthora capsici PC35 is 2.95 μg / mL, and the EC 50 value of atractylodes Phytophthora sansomei AMPH-1 is 2.39 μg / mL. The oxazolomycin A prepared by the application can be developed into a biological pesticide that can effectively meet the agricultural development demand of "green ecology", and provides key biocontrol resource support for green prevention and control of plant diseases.

This application belongs to the field of plant genetic engineering technology and specifically relates to a DpCYP707A2 gene for regulating dioscin synthesis and its applications. The nucleotide sequence of the DpCYP707A2 gene is shown in SEQ ID NO. 1. According to the results of the examples of the present invention, the DpCYP707A2 gene provided by the present invention can regulate the synthesis of dioscin in yam. The DpCYP707A2 gene plays an important role in influencing dioscin synthesis in plants and can help breed new yam varieties with increased dioscin content, thus having broad application value.

This invention relates to the field of biosynthesis technology, and particularly to engineered yeast strains and their application in the total fermentation production of steviol glycosides. This invention utilizes site-directed mutagenesis to mutate wild-type kaurenoic acid 13-hydroxylase, obtaining the G481L / Q159L mutant. This mutant is then applied to the production of steviol or steviol glycosides, constructing a steviol or steviol glycoside synthesis pathway in the *Saccharomyces cerevisiae* host to achieve efficient production of steviol or steviol glycosides. Simultaneously, by enhancing the level of diterpene precursor synthesis genes in chassis cells (MVA pathway, acetyl-CoA, GGPP, transport proteins, UDPG pathway), the yield of steviol glycosides is further increased, reducing costs for industrial production.

The invention relates to the technical field of bioengineering, in particular to a recombinant halophilic bacterium for producing PHBV by using acetate as a single carbon source and application of the recombinant halophilic bacterium. The expression and / or activity of 2-methyl citrate synthetase and succinate dehydrogenase assembly factor 2 of the recombinant halophilic bacteria provided by the invention are reduced, 3-hydroxyvaleric acid synthetic genes scpA and scpB are overexpressed, an ADP-dependent acetyl coenzyme A synthetase gene acd is overexpressed, and a heterologous monocarboxylic acid transporter gene mctC is expressed. The strain can generate PHBV without adding precursors such as propionic acid and valeric acid, acetic acid or acetate can be used as a sole carbon source to produce PHBV, the yield is close to that when glucose is used as a carbon source, and the problems that the added precursor substances have certain toxicity, so that the growth of thalli is influenced, and the yield is reduced are solved; environmental pollution caused by addition of precursor substances is avoided, and meanwhile, the fermentation production cost of the PHBV is effectively reduced.

Recombinant Sorangium cellulosum for producing de-epoxidized epothilone B by fermentation, insertional inactivation of an epoK gene in an epothilone biosynthetic gene cluster in the recombinant bacteria, and a method for producing de-epoxidized epothilone B using the recombinant bacteria.

The invention discloses streptomyces hygroscopicus ascomycetes subspecies and an obtaining method thereof. Regulating genes fkbN, fkbR1 and fkbR2 on an ascomycin biosynthesis gene cluster of the streptomyces hygroscopicus ascomycetes subspecies ATCC 14891 and a gene fkbS participating in synthesis of an ascomycin precursor ethyl malonyl coenzyme A are subjected to biosynthesis to obtain the streptomyces hygroscopicus ascomycetes subspecies. And carrying out overexpression on a regulatory gene SH03686 of a leucine response regulatory protein family (Lrp) outside the gene cluster, and screening an ascomycin high-yield strain. According to the invention, after combined overexpression of genes fkbS and fkbN and overexpression of a gene fkbR2 are carried out by a strain SN9 and a strain R2-17 in starting bacteria, the transcriptional level of regulatory genes (fkbN, fkbR2) on an ascomycin biosynthesis gene cluster and related genes participating in synthesis of precursors ethyl malonyl coenzyme A (fkbS, fkbE), piperidine acid (fkbL) and DHCHC (fkbO) are improved, and finally, the ascomycin yield is improved.

The invention belongs to the field of plant genetic engineering, and particularly relates to a soybean eugenol synthesis gene GmEGS and application thereof. The invention provides application of a soybean eugenol synthetic protein GmEGS or a nucleic acid molecule encoding the soybean eugenol synthetic protein GmEGS in increasing the content of eugenol in soybeans or improving the resistance of the soybeans to phytophthora sojae. The protein GmEGS comprises a protein GmEGS1a, a protein GmEGS1c and a protein GmEGS2a, and the amino acid sequences of the protein GmEGS1a, the protein GmEGS1c and the protein GmEGS2a are sequentially shown as SEQ ID NO.1-3; the ORF of the nucleic acid comprises nucleic acid molecules as shown in SEQ ID NO. 4-6. When the biological material disclosed by the invention is used for transforming soybean plants, the content of eugenol in soybeans can be obviously increased, and meanwhile, the disease resistance of the soybeans to phytophthora sojae can also be improved. The invention provides a good theoretical basis for a soybean disease-resistant molecular mechanism, and has important significance for molecular design breeding.

The invention provides amycolatopsis mediterranei with gene defects as well as a construction method and application of the amycolatopsis mediterranei. The first aspect of the invention provides a construction method of the amycolatopsis mediterranei with the gene defect, and the construction method comprises the step of knocking out a rifamycin biosynthetic gene cluster in a receptor amycolatopsis mediterranei strain U32 (Cas12a) genome and an integrated plasmid pDZLCas12a containing an FnCas12a coding gene to obtain the amycolatopsis mediterranei with the gene defect. According to the invention, a rifamycin biosynthetic gene cluster in a genome of an amycolatopsis mediterranei strain U32 (Cas12a) and an integrated plasmid pDZLCas12a containing an FnCas12a coding gene are knocked out, so that the amycolatopsis mediterranei with gene defects is constructed, and the amycolatopsis mediterranei can be used as a chassis strain for mining identified or unidentified biosynthetic gene clusters in actinomycetes.

This invention provides Bacillus subtilis ( Bacillus subtle The application of strain A45 in the control of plant diseases, its application in the preparation of microbial agents for the control of plant diseases, and the prepared microbial agents. Bacillus subtilis A45 is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33289. The liquid microbial agent containing Bacillus subtilis A45 provided by this invention effectively inhibits the cucumber target spot pathogen, *Clostridium multiflorum* (…). Corynespora cassicola The growth mechanism of this strain lies in its encoding a gene cluster for the synthesis of phytosalicylic acid, purchemin, subtilosin A, and surfactant. Among these, the surfactant synthesis gene cluster is unique to this strain, exhibiting a maximum sequence similarity of 82%. Experiments show that the liquid inoculant containing Bacillus subtilis A45 provided by this invention can effectively inhibit the growth of *Clostridium multiflorum*, the pathogen causing target spot disease in cucumbers. Corynespora cashew nut The growth of ) can be controlled by field application with a prevention and control effect of up to 80%.

The invention belongs to the technical field of plant genetic engineering, and discloses a gene for regulating and controlling the endogenous salicylic acid content and disease resistance of rice by using an SA synthetic gene OSD3 participating in rice PAL dependence and application of the gene, and the nucleotide sequence of the OSD3 is SEQ ID NO: 1; the nucleotide sequence of a coding protein region is SEQ ID NO: 2; the amino acid sequence of the coded protein is SEQ ID NO: 3; according to the invention, an OSD3 gene is knocked out through CRISPR / Cas9 under the background of Zhonghua 11, a mutant osd3 of which the SA of a rice leaf is reduced to 20% of that of a wild type is obtained, and an OSD3 overexpression plant is created by constructing an OSD3-pMDC43 overexpression vector.

This invention belongs to the field of biotechnology, specifically relating to neuropeptides from the root-knot nematode *Euonymus alatus*. nlp12 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Heliotropium indicum* root-knot nematodes, this invention is based on... nlp12 The nucleotide sequence of the gene is SEQ ID NO:1. Gene fragment SEQ ID NO:5 and the corresponding dsRNA were designed and synthesized. The synthesized dsRNA was used to soak second-instar root-knot nematodes. nlp12 Gene expression levels were downregulated, resulting in a mortality rate of 73.2% at 60 hours, and complete mortality of nematodes at 108 hours. Immersion nlp12 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 54%, and the number of female nematodes, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.

The application discloses a rice terpene phytoalexin synthesis gene OsCPS4 and application, relates to the field of plant genetic engineering, and the nucleotide sequence of the gene is shown as SEQ ID NO. 1. The application is a good example that the rice terpene phytoalexin participates in crop insect resistance, has certain reference value for understanding the regulation of the rice terpene phytoalexin on pest resistance, and OsCPS4 the research on the gene provides a good theoretical basis for the molecular mechanism of the rice brown plant hopper resistance gene, and has important significance for molecular design breeding.

The invention belongs to the technical field of plant genetic engineering and the technical field of soybean breeding, and particularly discloses application of a soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number, the CDS sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.1, the amino acid sequence of the encoded protein is shown as SEQ ID No.2, and the amino acid sequence of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C is shown as SEQ ID No.3. The invention further discloses application of the soybean brassinolide inactivating enzyme encoding gene GmBAS1C in regulation and control of the soybean branch number. The gene is located in an endoplasmic reticulum, and formation and development of lateral branches are promoted by up-regulating expression quantities of genes such as brassinolide synthesis genes CPD, ROT3 and BR6OX2, auxin response genes IAA, GH3 and SAUR and the like. The invention also discloses three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene, a soybean plant containing the gene editing mutants, and a vector construction method of the soybean plant. The invention further discloses the three homozygous gene editing mutants (KOGmBAS1C-1, KOGmBAS1C-2 and KOGmBAS1C-3) of the GmBAS1C gene. Experiments prove that the number of branches of homozygous gene editing mutant phenotypes of the three GmBAS1C genes is remarkably increased. The invention provides a new gene target, a molecular tool and a breeding material for soybean high-branch breeding, and has important agricultural application value.

The invention provides an escherichia coli self-regulation anti-acid module and application thereof. The self-regulation anti-acid module is composed of an acid response promoter, an anti-acid system gene, an ATP synthesis promoting gene and a terminator. Compared with a wild strain, the self-regulation acid-resistant module constructed by the invention can effectively improve the acid stress resistance of recombinant escherichia coli, and the growth ability of the strain containing the self-regulation acid-resistant module under an acidic condition is improved by 72.4%.

This invention discloses a genetically engineered bacterium for producing rhamnolipids and its applications, belonging to the field of gene recombination and metabolic engineering technology. The genetically engineered bacterium for producing rhamnolipids disclosed in this invention uses *Yarrowia lipolyticis* ATCC MYA-2613 as the starting strain and heterologously expresses the rhamnolipid synthesis gene. rhlA, rhlB and rhlC, and dTDP-L-rhamnose synthesis gene rmlA, rmlB, rmlC and rmlD This invention discloses a genetically engineered bacterium for producing rhamnolipin, which can efficiently metabolize glucose or glucose-containing cellulose hydrolysates to produce rhamnolipin, achieving a rhamnolipin yield of 42.8 g / L. The genetically engineered bacterium constructed in this invention provides a possibility for the efficient production of rhamnolipin from glucose or glucose-containing cellulose hydrolysates.

The application discloses recombinant saccharomyces cerevisiae for producing wax melia oil and a construction method and application thereof, and belongs to the technical field of biology. By modular integration and optimization of terpene precursor supply and gene expression, the galactokinase genes GAL1, GAL7 and GAL10 are knocked out in the original saccharomyces cerevisiae, and terpene precursor synthesis genes and wax melia oil characteristic terpene synthesis enzyme genes are introduced, so that the recombinant saccharomyces cerevisiae is obtained, that is, a wax melia oil production strain. The proportion of elemol in the fermentation product of the recombinant strain is as high as 51.0%, and six target terpene components are determined. The application provides a stable biological product strain for the application of wax melia oil in the fields of spices, cosmetics and medicines, and has significant economic value and practical application value.

This invention relates to the field of plant genetic engineering technology, and discloses an AtaSQS gene for the biosynthesis of triterpenoids in Aster tataricus and its applications. The AtaSQS gene includes a complete coding region CDS sequence and a promoter sequence, derived from *Aster tataricus* var. *ji*. The CDS sequence is shown in SEQ ID NO.1. The promoter sequence, 1289 bp in length, is located upstream of the CDS sequence and is used to regulate the spatiotemporally specific expression of the AtaSQS gene. This invention cloned the key gene AtaSQS for the biosynthesis of triterpenoids and its promoter sequence from *Aster tataricus* var. *ji*. It clarified its positive regulatory function on the synthesis of total triterpenoids and asterones, and provided related recombinant expression vectors, host bacteria, and breeding methods. This fills the technical gap in the regulatory mechanism of triterpenoid synthesis in Aster tataricus, and can significantly accelerate the breeding process of Aster tataricus varieties with high triterpenoid content, improve their medicinal quality and economic value, and has important application prospects in the fields of plant genetic engineering and the quality improvement of traditional Chinese medicinal materials.

The invention relates to heat-resistant yeast for synthesizing mannitol as well as a construction method and application of the heat-resistant yeast. According to the invention, firstly, Kluyveromyces marxianus is used as a chassis to screen five kinds of mannitol synthesis related genes from different sources, wherein the mannitol yield of the strain is the highest when CmMDH is over-expressed. And then, promoting the expression of the NADPH synthetic gene by increasing the copy number of the CmMDH and overexpression, so as to obtain the strain Y2CmMDH-KmUTR1 with higher yield. The production of the mannitol is further promoted by subsequently improving the oxygen introduction and pH (Potential of Hydrogen) environment. According to the invention, the yeast strain for efficiently producing the mannitol at 42 DEG C is constructed for the first time, and the mannitol is produced by using the molasses and the jerusalem artichoke without adding extra enzymes. The method has important significance for realizing industrial production of mannitol and recycling of agricultural wastes through a microbial synthesis method.

The invention provides a rabbit polyclonal antibody aiming at primary cilia and a preparation method thereof, and belongs to the technical field of biology, and the preparation method comprises the following steps: taking a 200-428 amino acid sequence of human ARL13B protein as a target fragment, synthesizing a gene after codon optimization, cloning the gene to a pet-32a expression vector, and carrying out induced expression in escherichia coli to obtain the rabbit polyclonal antibody aiming at primary cilia. Purifying to obtain high-purity immunogen protein; the nucleotide sequence of the synthetic gene is as shown in SEQ ID NO.1, and the amino acid sequence of the high-purity immunogen protein is as shown in SEQ ID NO.2. Immunizing a New Zealand white rabbit with the high-purity immunogen protein to obtain immune serum; and purifying the immune serum to obtain the rabbit polyclonal antibody. The prepared rabbit polyclonal antibody has higher specificity and signal strength, the titer can reach 1: 64000 or above through ELISA detection, primary cilia can be marked more clearly in a paraffin section immunofluorescence experiment, background noise is remarkably reduced, and the reliability of an experiment result is improved.

This invention relates to the field of gene technology, specifically disclosing a method for enhancing the secretion of secondary metabolites based on the regulation of quorum sensing signal molecules. This method activates the expression of bacterial secondary metabolite synthesis gene clusters through bacterial-fungal interaction, comprising the following steps: (a) co-culturing bacteria and fungi in contact or non-contact mode; (b) during co-culturing, the fungi secrete the quorum sensing signal molecule farnesol; (c) the bacteria sense farnesol through a two-component system, activating the expression of secondary metabolite synthesis genes. This invention effectively overcomes the gene silencing problem caused by metabolic load or lack of regulation in natural strains by activating the originally trace expression of secondary metabolite synthesis gene clusters in bacteria using the fungal-derived quorum sensing signal molecule farnesol.

This invention discloses the chitin synthase 1 (LmCHS1) gene of the leafminer moth, its dsRNA, its synthesis method, and its insect-resistant applications. Through transcriptome analysis, specific primer pairs for the open reading frame of the LmCHS1 gene were designed and synthesized. The LmCHS1 gene sequence was obtained through gene cloning (nucleotide sequence: SEQ ID NO: 1, amino acid sequence: SEQ ID NO: 2). The gene fragment with sequence SEQ ID NO: 3 was selected as the RNAi target sequence. Primer pairs SEQ ID NO: 6 and SEQ ID NO: 7 with restriction enzyme sites were designed and synthesized. PCR amplification was performed, and a prokaryotic expression vector was constructed to induce dsRNA expression. Feeding leafminer moth larvae with dsRNA interfered with LmCHS1 gene expression. After 24 hours, abnormalities were observed in the larvae's body surface, with some larvae failing to complete molting or dying due to an inability to form a normal body surface after molting. After 96 hours, the larval mortality rate was 72.22%, compared to 40% in the GFP control group, demonstrating a significant effect. This invention has broad application prospects in the control of the leaf borer pest in forestry due to its high efficiency, specificity, and safety.

The present invention refers to the heterologous production in microorganisms of modified versions of a predicted isoform of the surfactant protein Lv-ranaspumin-1 (Lv-Rsn-1), whose sequence was inferred from analyzes of the protein extract of the nest foam from the Northeastern Pepper Frog (Leptodactylus vastus). More specifically, it refers to two surfactant proteins that consist of modified versions of the predicted isoform of Lv-Rsn-1; to two synthetic genes each encoding one of these modified versions of the predicted isoform of Lv-Rsn-1; to two expression cassettes each containing one of the synthetic genes encoding one of the modified versions of the predicted isoform of Lv-Rsn-1; to two expression vectors each containing one of the synthetic genes encoding modified versions of the predicted isoform of Lv-Rsn-1; and to two transgenic microorganisms, a bacterium and a yeast, each transformed with one of these synthetic genes and heterologously producing one of the modified versions of the predicted isoform of Lv-Rsn-1. Lv-Rsn-1 has surfactancy, emulsification and dispersancy properties, among others, and its heterologous production allows it to be used in various applications and industrial products, without the need to extract it from the frog nest foam.

The invention discloses a method for improving whole-cell catalytic synthesis of N-acetylneuraminic acid (NeuAc) by bacillus amyloliquefaciens engineering bacteria through metabolic pathway optimization. According to the method, NeuAc synthesis precursor N-acetylglucosamine (GlcNAc) decomposition pathway genes nagA and nagB and a phosphate transport pathway gene nagP in bacillus amyloliquefaciens are knocked out through a genetic engineering method, meanwhile, a NeuAc synthesis precursor pyruvic acid competition pathway phosphate oxidase gene ydaP is knocked out, on the basis, a NeuAc key synthesis gene age or shnano A is further subjected to heterologous expression, and the NuAc synthesis precursor gene nagA or shnano B is obtained. Two strains of bacillus amyloliquefaciens engineering bacteria are obtained; the two engineering bacteria are used as catalysts, GlcNAc and sodium pyruvate are used as substrates, a whole-cell catalytic reaction is carried out, the yield of NeuAc reaches 134.68 g / L, the conversion rate of the GlcNAc is increased by 96% compared with that of a control bacterium, and the yield of NeuAc and the utilization rate of the substrates are remarkably increased.

The application relates to application of itaconic acid synthesis gene Irg1 in preparation of a product for improving the resistance of tilapia to edwardsiella, and belongs to the field of genes. The nucleotide sequence of the itaconic acid synthesis gene Irg1 is SEQ ID NO. 1. In the application process, the itaconic acid synthesis gene Irg1 is overexpressed to synthesize itaconic acid, improve the disease resistance of tilapia to edwardsiella, kill bacteria in the body of the tilapia, and guarantee the integrity of intestinal tissues.

The application discloses application of a TPP riboswitch sequence editing material in synchronously improving crop stress resistance, yield and nutritional quality, and belongs to the technical field.The TPP riboswitch gene belongs to a crop vitamin B1 synthesis gene THIC;the TPP riboswitch sequence editing material comprises: an sgRNA targeting a TPP riboswitch sequence in a 3'-UTR of the THIC gene, a recombinant vector containing the sgRNA, and a recombinant microorganism containing the recombinant vector.The application edits the TPP riboswitch sequence of a plant through CRISPR-Cas9 technology, improves the TPP level, and thus simultaneously improves crop yield, nutritional quality and adaptability to stress.The application provides a very effective strategy for simultaneously improving crop yield, nutritional quality and stress resistance.

The invention belongs to the technical field of gene engineering, particularly relates to a rubber tree CRISPR (clustered regularly interspaced short palindromic repeats) transcriptional activation technology, more particularly relates to a rubber tree CRISPR transcriptional activation vector, and further discloses a construction method and application of the rubber tree CRISPR transcriptional activation vector. The hevea brasiliensis CRISPR transcription activation technology is constructed for the first time, dCas9TV is targeted to a hevea brasiliensis endogenous gene promoter region through specific sgRNA, transcription of a downstream target gene is activated, and the hevea brasiliensis anthocyanin synthesis transcription factor gene HbAN2 is further selected as a target gene to verify the technology. Screening and synthesizing a high-efficiency specific target site sequence on an HbAN2 gene promoter, and constructing the high-efficiency specific target site sequence to the downstream of an HbU6.2 promoter of an Hb-dCas9TV vector. The CRISPR transcriptional activation vector of the rubber tree can promote synthesis and accumulation of anthocyanin in a transgenic embryo block to be red, so that efficient visual screening of effective transcriptional activation materials is realized.