35 results about "Cellular Regulation" patented technology

While genetic engineering has undergone rapid advancement with the discovery of CRISPR / Cas9, there is room for improvement for genetic circuit control, precision (reducing circuit ‘leakiness’) and delivery into living systems. The claimed invention offers programmable and precise regulation of dCas9 functions in response to multiple molecular signals by using synthetic gene circuits, greatly expanding applications. Moreover, using the system to greatest therapeutic potential has been greatly limited by the restrictive cargo size of existing viral delivery systems. By splitting dCas9 into multiple sections, the delivery size of synthetic gene circuits is greatly reduced. By exchanging split dCas9 domains, differential regulation on one gene, or activating two different genes in response to cell-type specific microRNAs is illustrated. Practical applications of the illustrative examples include engineered sensory switches including indicators for bladder cancer as well as enhanced systems for adenovirus delivery, cellular regulation, plant cell modification and potential therapeutic applications.

The present invention provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides reagents for use in an automated environment for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample.

Methods and materials to modulate the immune response to treat or prevent a disease or to prevent transplant rejection, including methods of making T helper-antigen presenting cells and / or T regulatory-antigen specific cells and methods of using these cells. The invention also relates to methods of making exosome-absorbed dendritic cells and the uses of these cells to modulate the immune response to treat or prevent a disease or to prevent transplant rejection.

Provided herein are reagents for use in an automated environment for cell conditioning of biological samples wherein the cells or tissues are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents include, but are not limited to, components optimized to facilitate molecular access to cells and cell constituents within the biological sample. Also provided herein are reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents include, but are not limited to, components optimized to facilitate removal or etching of the embedding media from the biological sample.

The invention discloses a culture solution for inducing mesenchymal stem cells to differentiate into islet-like cells, and an inducing method and application of the culture solution. The culture solution comprises the following materials: niacinamide, Conophylline, cell growth factor, betacellulin and a base medium. The base medium contains 97% of high glucose DMEM (Dulbecco Modified Eagle Medium), 2% of B-27 and 1% of N-2. The inducing method comprises the following steps of: preparing an inducing culture solution; preparing the human mesenchymal stem cells; taking the human mesenchymal stem cells, inoculating the human mesenchymal stem cells into a six-hole ultralow absorption culture plate by 1.5-2*105cells / hole, adding 3ml inducing culture medium into each hole and carrying out suspended induction; and changing liquid at every 3 days, collecting cell supernatant at the ninth day, and storing the cell supernatant at the temperature of -20 DEG C. The culture solution disclosed by the invention has the advantages that the human mesenchymal stem cells are induced to differentiate into the islet-like cells by utilizing the combination of the niacinamide and the Conophylline, so that the inducing cycle is shortened, the suspension cells are beneficial to being clustered to form cell clusters similar to natural islets, further the induced differentiation efficiency is obviously increased and the clinical application risk is reduced; and the function of inducing the secretion of the cell insulin is obviously improved.

The invention provides the application of transcription factor ZHX2 in the regulation of NK cells, and belongs to the technical fields of functional genomics and biomedicine. The present invention confirms through research that ZHX2 gene can inhibit the killing function of NK cells by down-regulating the expression of the important activating receptor NKG2D on the surface of NK cells, inhibiting the degranulation ability of NK cells and the secretion of cytokines. Therefore, it can be used for gene diagnosis and gene therapy of human autoimmune diseases and tumors, and can also be used for the design and development of new drugs for the ZHX2 gene. Therefore, it has good practical application value.

The invention relates to the field of wound repair materials, and provides a preparation method of a wound repair material with the function of regulating cell growth, including: S1. adding high molecular polymer and nano-bioactive glass powder to a solvent, and preparing electrospinning Precursor solution; S2. Inhale the electrospinning precursor solution configured in step S1 into the syringe for electrospinning process, vacuum drying, and obtain a polymer fiber film compounded with nano-bioactive glass; S3. Extract cell regulatory factors targeting Gene fragments, constructing target gene plasmids, using genetic recombination technology to obtain plasmid DNA recombinants, and adding plasmid DNA recombinants to chitosan solution and trehalose solution to obtain a mixed solution; S4. The mixed solution obtained in step S3 Coated on the polymer fiber film obtained in step S2, the wound repair material prepared by the present invention has better tissue compatibility, tensile strength, biological safety, antibacterial property and repair ability.

The invention provides the application of telomere binding protein DAXX in preparing tumor cell regulator. The telomere-associated protein DAXX includes an ATRX binding domain, a histone binding domain, a telomerase binding domain, a Cajal body binding domain and a telomere binding domain. DAXX can be located on the telomeres of telomerase-positive tumor cells, and maintain the length of telomeres by regulating the assembly and transport of telomerase, thereby regulating the growth and reproduction of tumor cells. In addition, DAXX can also be located on the telomeres of tumor cells independent of the telomerase-independent telomere elongation mechanism (ALT), and regulate the length of telomeres by affecting the ALT mechanism. Therefore, DAXX has important telomere regulation functions in telomerase-positive tumor cells and ALT mechanism-existing tumor cells, and effective tumor cell regulators can be developed for the regulation mechanism of DAXX on tumor cells.

A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.

The invention discloses a targeted photodynamic nanoprobe based on upconversion nanoparticles and an ultrathin silicon dioxide layer. The nanoprobe comprises an upconversion nanoparticle core and ultrathin silicon dioxide wrapped on the surface of the core A shell, methylene blue loaded on said shell, and polyethylene glycol-folate. Constructed UCNPs@SiO 2 The / MB / PEG-FA nanoprobe is distributed in the cytoplasm of cancer cells through receptor-mediated endocytosis, and generates reactive oxygen species under the excitation of near-infrared light, which reduces the mitochondrial membrane potential in cells and induces irreversible cell regulation in cancer cells. Death. Constructed UCNPs@SiO 2 The / MB / PEG‑FA nanoprobe also exhibited excellent tumor-inhibitory effects in animal experiments.

The invention provides an identification method, device, equipment and storage medium of a single-cell miRNA regulatory network, and relates to the technical field of genetic engineering. The identification method of the single-cell miRNA regulatory network comprises: obtaining single-cell transcriptome data of miRNAs and target genes of matched samples. The number of matching samples is obtained. If the number of matching samples is less than the preset threshold, a pseudo single cell is inserted into the matching samples by an interpolation method to obtain an expanded matching sample, and the matching sample is updated to the expanded matching sample. According to the statistical correlation value of miRNA and target gene, calculate the significance value of the association relationship between miRNA and target gene, and determine whether there is a regulatory relationship between miRNA and target gene in a single cell according to the significance value. The regulatory relationship between miRNAs and target genes determined from the single-cell transcriptome data of matched samples is fused to obtain a single-cell miRNA regulatory network. Realize the ability to construct the miRNA regulatory network of each single cell when not enough matching samples can be obtained.