35 results about "Cellular Regulation" patented technology

The present invention provides reagents for use in an automated environment for removing or etching embedding media by exposing a biological sample to be stained in histochemical or cytochemical procedures without the dependence on organic solvents. The reagents comprise components optimized to facilitate removal or etching of the embedding media from the biological sample. The present invention also provides reagents for use in an automated environment for cell conditioning biological samples wherein the cells are predisposed for access by reagent molecules for histochemical and cytochemical staining procedures. The reagents comprise components optimized to facilitate molecular access to cells and cell constituents within the biological sample.

The invention discloses a culture solution for inducing mesenchymal stem cells to differentiate into islet-like cells, and an inducing method and application of the culture solution. The culture solution comprises the following materials: niacinamide, Conophylline, cell growth factor, betacellulin and a base medium. The base medium contains 97% of high glucose DMEM (Dulbecco Modified Eagle Medium), 2% of B-27 and 1% of N-2. The inducing method comprises the following steps of: preparing an inducing culture solution; preparing the human mesenchymal stem cells; taking the human mesenchymal stem cells, inoculating the human mesenchymal stem cells into a six-hole ultralow absorption culture plate by 1.5-2*105cells / hole, adding 3ml inducing culture medium into each hole and carrying out suspended induction; and changing liquid at every 3 days, collecting cell supernatant at the ninth day, and storing the cell supernatant at the temperature of -20 DEG C. The culture solution disclosed by the invention has the advantages that the human mesenchymal stem cells are induced to differentiate into the islet-like cells by utilizing the combination of the niacinamide and the Conophylline, so that the inducing cycle is shortened, the suspension cells are beneficial to being clustered to form cell clusters similar to natural islets, further the induced differentiation efficiency is obviously increased and the clinical application risk is reduced; and the function of inducing the secretion of the cell insulin is obviously improved.

This invention provides methods of generating induced sensory neurons (iSNs) from non-neuronal cells such as fibroblasts. The invention also provides methods of using iSNs in various therapeutic or non-therapeutic applications, e.g., methods to identify agents or cellular modulations that enhance iSN formation from non-neuronal cells.

The invention provides the application of transcription factor ZHX2 in the regulation of NK cells, and belongs to the technical fields of functional genomics and biomedicine. The present invention confirms through research that ZHX2 gene can inhibit the killing function of NK cells by down-regulating the expression of the important activating receptor NKG2D on the surface of NK cells, inhibiting the degranulation ability of NK cells and the secretion of cytokines. Therefore, it can be used for gene diagnosis and gene therapy of human autoimmune diseases and tumors, and can also be used for the design and development of new drugs for the ZHX2 gene. Therefore, it has good practical application value.

The invention discloses a targeted photodynamic nanoprobe based on upconversion nanoparticles and an ultrathin silicon dioxide layer. The nanoprobe comprises an upconversion nanoparticle core and ultrathin silicon dioxide wrapped on the surface of the core A shell, methylene blue loaded on said shell, and polyethylene glycol-folate. Constructed UCNPs@SiO 2 The / MB / PEG-FA nanoprobe is distributed in the cytoplasm of cancer cells through receptor-mediated endocytosis, and generates reactive oxygen species under the excitation of near-infrared light, which reduces the mitochondrial membrane potential in cells and induces irreversible cell regulation in cancer cells. Death. Constructed UCNPs@SiO 2 The / MB / PEG‑FA nanoprobe also exhibited excellent tumor-inhibitory effects in animal experiments.

The invention provides an identification method, device, equipment and storage medium of a single-cell miRNA regulatory network, and relates to the technical field of genetic engineering. The identification method of the single-cell miRNA regulatory network comprises: obtaining single-cell transcriptome data of miRNAs and target genes of matched samples. The number of matching samples is obtained. If the number of matching samples is less than the preset threshold, a pseudo single cell is inserted into the matching samples by an interpolation method to obtain an expanded matching sample, and the matching sample is updated to the expanded matching sample. According to the statistical correlation value of miRNA and target gene, calculate the significance value of the association relationship between miRNA and target gene, and determine whether there is a regulatory relationship between miRNA and target gene in a single cell according to the significance value. The regulatory relationship between miRNAs and target genes determined from the single-cell transcriptome data of matched samples is fused to obtain a single-cell miRNA regulatory network. Realize the ability to construct the miRNA regulatory network of each single cell when not enough matching samples can be obtained.