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Preparation method of 3D printing bio-ink material with controllable material distribution and preparation method of three-dimensional bionic hydrogel scaffold

A bio-ink and 3D printing technology, applied in the field of biological 3D printing, can solve the problems of difficult to regulate cell behavior and prepare functional tissues, and achieve the effect of personalized customization, controllability and simple production process

Active Publication Date: 2022-05-13
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that traditional tissue engineering scaffolds mainly focus on providing temporal and spatial position resolution for cell growth, and it is difficult to regulate cell behavior and prepare functional tissues, and provide a 3D printed biological material with controllable distribution. Ink material production method and three-dimensional biomimetic hydrogel scaffold preparation method, this method realizes the personalized customization and high-precision control of material distribution through customizing molds, introducing coating materials and demolding, and endows 3D printing with unique features. Good three-dimensional structure of the scaffold

Method used

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  • Preparation method of 3D printing bio-ink material with controllable material distribution and preparation method of three-dimensional bionic hydrogel scaffold
  • Preparation method of 3D printing bio-ink material with controllable material distribution and preparation method of three-dimensional bionic hydrogel scaffold
  • Preparation method of 3D printing bio-ink material with controllable material distribution and preparation method of three-dimensional bionic hydrogel scaffold

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specific Embodiment approach 1

[0028] Specific embodiment one: what this embodiment records is a kind of 3D printing bio-ink material preparation method with material controllable distribution, described method comprises the following steps:

[0029] Step 1: 3D print and customize a high-precision mold, where the mold fits with the inner surface of the syringe (not a tight fit, there are some random gaps);

[0030] Step 2: Prepare cold-induced hydrogel with temperature-sensitive properties and a dynamic viscosity of 3000-25000 Pa.s at room temperature and thermal-induced hydrogel with 1-500 Pa.s, and use the thermal-induced hydrogel as the mold surface coating Layer material; the surface coating of the mold means that not all surfaces are coated, but in fact only partial coating. To achieve the fit is to use the uncoated mold surface to produce a clearance fit with the inner surface of the syringe. This clearance fit acts as a fixed mold, not for demoulding between the outer layer of cold-induced hydrogel a...

specific Embodiment approach 2

[0032] Specific embodiment two: a kind of 3D printing bio-ink material preparation method with material controllable distribution described in specific embodiment one, in step 1, the shape of mold directly affects the material distribution of described 3D printing bio-ink material, 3D Printing uses a high-precision resin 3D printer with a printing accuracy of not less than 0.05mm.

specific Embodiment approach 3

[0033] Embodiment 3: A method for manufacturing a 3D printing bio-ink material with controllable distribution of materials described in Embodiment 1. In step 1, the mold pattern of the 3D printing is adjusted according to the preset pattern of bio-ink material distribution Personalized customization; the matching gap of the gap is +0.01 ~ +0.1mm, so as to ensure the fixation of the mold on the inner surface of the syringe and the insertion and removal of the mold during the production process of the bio-ink material.

[0034]Embodiment 4: A method for manufacturing a 3D printing bio-ink material with controllable distribution of materials described in Embodiment 1. In step 2, the thermogenic hydrogel is Pluronic F127, and the cold-induced hydrogel It is a composite hydrogel of gelatin and sodium alginate or methacrylamide gelatin and sodium alginate. Pluronic F127 is opposite to the temperature-sensitive properties of the cryotropic hydrogel. Adjusting the temperature can not ...

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Abstract

The invention relates to a manufacturing method of a 3D printing bio-ink material with controllable material distribution and a preparation method of a three-dimensional bionic hydrogel scaffold, the controllable material distribution relates to controllable material pattern and component distribution, and the method comprises the following steps: S1, 3D printing customizing a high-precision patterned mold in clearance fit with the inner surface of a 3D printer needle cylinder, the template is used for regulating and controlling material distribution; s2, preparing thermally induced hydrogel and cold induced hydrogel which are relatively high in concentration and have temperature-sensitive characteristics, wherein the thermally induced hydrogel is used as a mold surface coating material; and S3, preparing the bio-ink material with controllable material distribution. According to the method, the coating material is utilized, and the manufacturing method of placing the coating material into the mold, pouring the outer layer cold-induced hydrogel, demolding and pouring the inner layer cold-induced hydrogel is combined, so that personalized customization and high-precision regulation and control of material distribution are well realized, and a good three-dimensional structure is given to the 3D printed heterogeneous stent. In addition, in combination with cell biochemical factors, the alginate-based hydrogel scaffold can be further endowed with cell regulation and control capability.

Description

technical field [0001] The invention belongs to the technical field of biological 3D printing, and in particular relates to a method for manufacturing a 3D printing bio-ink material with controllable material distribution and a method for preparing a three-dimensional bionic hydrogel support. Background technique [0002] At present, many complex human organs still lack clinically available substitutes. The tissue engineering method adopts adding scaffolds, growth factors and cells to the culture medium for in vitro synthesis, or implanting scaffolds and growth factors into the body for synthesis, which provides a way for tissue and organ repair. 3D printing technology adopts the idea of ​​discrete / stacking, which can produce entities with arbitrary complex internal structures. It can not only realize the preparation of different material components and complex structures on the same slice, but also the components and structural forms on different slices can be changed arbit...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/20A61L27/52A61L27/50C08J3/075C08L89/00C08L5/04
CPCA61L27/222A61L27/20A61L27/52A61L27/50C08J3/075C08J2389/00C08J2405/04C08L5/04
Inventor 孙雅洲王鹏举刘海涛张博汉
Owner HARBIN INST OF TECH
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