Preparation method of antibacterial stent for bone repair

Abstract

The invention discloses a preparation method of an antibacterial stent for bone repair. Firstly, gelatin and sodium alginate are dissolved in ultrapure water to obtain gelatin / sodium alginate slurry;58 s bioactive glass and the gelatin / sodium alginate slurry are mixed and uniformly stirred, the mixture is placed in a 3D printing barrel for defoaming to obtain gelatin / sodium alginate / bioglass composite slurry; the slurry is printed into a stent adopting a 3D porous structure by a 3D printer, and freeze drying treatment is performed to obtain a gelatin / sodium alginate / bioglass composite bone repair stent; the composite bone repair stent is placed in a polydopamine solution for reaction overnight, and placing a product in a silver nitrate solution after reaction; the stent is washed by deionized water, and freeze drying is carried out to obtain the antibacterial stent for bone repair. The antibacterial stent can have antibacterial and anti-infective functions while performing bone repair, the materials are widely sourced, the materials are good in mechanical properties and high in bioactivity, and the preparation process is simple and easy to operate and has broad application prospects in fields of regenerative medicine and bone repair.

Description

technical field

[0001] The invention relates to the technical field of bone tissue engineering repair and reconstruction, in particular to a preparation method of an antibacterial bracket for bone repair. Background technique

[0002] With the increase of bone tissue damage caused by aging, degenerative diseases, traffic accidents and other traumas, more and more attention has been paid to the repair of bone defects. In clinical practice, bone transplantation methods such as autologous bone transplantation, allogeneic bone transplantation and artificial bone transplantation are usually used. Autologous bone grafting is the "gold standard" for defect repair, but the source of autologous bone is limited, often in short supply, allograft bone graft has the risk of infection and disease, and artificial bone lacks osteoinductive activity, these shortcomings and shortcomings limit its clinical application. Therefore, the study of new regenerative bone defect repair materials with ...

Examples

Embodiment 1

[0029] 1) Add 1.2g of sodium alginate powder into 30ml of ultrapure water, stir for 12 hours, and wait until it is fully dissolved to obtain a sodium alginate solution.

[0030] 2) Add 1.8 g of gelatin powder into the sodium alginate solution and stir for 2 hours to obtain a gelatin / sodium alginate composite slurry.

[0031] 3) Prepare 58s bioactive glass by sol-gel method, add 0.34g of bioactive glass to gelatin / sodium alginate composite slurry (bioglass content is 10%), stir for 6h until mixed evenly, and obtain gelatin / alginic acid Sodium / bioglass composite slurry.

[0032] 4) Pour the gelatin / sodium alginate / biological glass composite slurry obtained in step 3) into a 3d printing barrel for degassing, model in a 3d printer, and print the slurry into a bracket, wherein the size of the bracket is set to 10mm*10mm*4mm, the diameter of the printing needle is 0.41mm, the printing speed is 15mm / s, and the printing pressure is 1.5bar.

[0033] 5) Carry out cross-linking treatme...

Embodiment 2

[0037] 1) Add 1.8g of sodium alginate powder into 30ml of ultrapure water, stir for 15 hours, and wait until it is fully dissolved to obtain a sodium alginate solution.

[0038] 2) Add 3.0 g of gelatin powder into the sodium alginate solution and stir for 2 hours to obtain a gelatin / sodium alginate composite slurry.

[0039] 3) Prepare 58s bioactive glass by sol-gel method, add 2.06g of bioactive glass into the gelatin / sodium alginate composite slurry (the content of bioglass is 30%), stir for 4h until the mixture is uniform, and obtain gelatin / alginic acid Sodium / bioglass composite slurry.

[0040] 4) Pour the gelatin / sodium alginate / biological glass composite slurry obtained in step 3) into a 3d printing barrel for degassing, model in a 3d printer, and print the slurry into a bracket, wherein the size of the bracket is set to 10mm*10mm*4mm, the diameter of the printing needle is 0.41mm, the printing speed is 20mm / s, and the printing pressure is 1.0bar.

[0041] 5) Carry ou...

Embodiment 3

[0045] 1) Add 2.4g of sodium alginate powder into 30ml of ultrapure water, stir for 18 hours, and wait until it is fully dissolved to obtain a sodium alginate solution.

[0046] 2) Add 1.2 g of gelatin powder into the sodium alginate solution and stir for 4 hours to obtain a gelatin / sodium alginate composite slurry.

[0047] 3) Prepare 58s bioactive glass by sol-gel method, add 3.6g of bioactive glass to gelatin / sodium alginate composite slurry (bioglass content is 50%), stir for 6h until mixed evenly, and obtain gelatin / alginic acid Sodium / bioglass composite slurry.

[0048] 4) Pour the gelatin / sodium alginate / biological glass composite slurry obtained in step 3) into a 3d printing barrel for degassing, model in a 3d printer, and print the slurry into a bracket, wherein the size of the bracket is set to 10mm*10mm*4mm, the diameter of the printing needle is 0.41mm, the printing speed is 30mm / s, and the printing pressure is 3.0bar.

[0049] 5) Carry out cross-linking treatmen...

technical field

[0001] The invention relates to the technical field of bone tissue engineering repair and reconstruction, in particular to a preparation method of an antibacterial bracket for bone repair. Background technique

[0002] With the increase of bone tissue damage caused by aging, degenerative diseases, traffic accidents and other traumas, more and more attention has been paid to the repair of bone defects. In clinical practice, bone transplantation methods such as autologous bone transplantation, allogeneic bone transplantation and artificial bone transplantation are usually used. Autologous bone grafting is the "gold standard" for defect repair, but the source of autologous bone is limited, often in short supply, allograft bone graft has the risk of infection and disease, and artificial bone lacks osteoinductive activity, these shortcomings and shortcomings limit its clinical application. Therefore, the study of new regenerative bone defect repair materials with ...

Examples

Embodiment 1

[0029] 1) Add 1.2g of sodium alginate powder into 30ml of ultrapure water, stir for 12 hours, and wait until it is fully dissolved to obtain a sodium alginate solution.

[0030] 2) Add 1.8 g of gelatin powder into the sodium alginate solution and stir for 2 hours to obtain a gelatin / sodium alginate composite slurry.

[0031] 3) Prepare 58s bioactive glass by sol-gel method, add 0.34g of bioactive glass to gelatin / sodium alginate composite slurry (bioglass content is 10%), stir for 6h until mixed evenly, and obtain gelatin / alginic acid Sodium / bioglass composite slurry.

[0032] 4) Pour the gelatin / sodium alginate / biological glass composite slurry obtained in step 3) into a 3d printing barrel for degassing, model in a 3d printer, and print the slurry into a bracket, wherein the size of the bracket is set to 10mm*10mm*4mm, the diameter of the printing needle is 0.41mm, the printing speed is 15mm / s, and the printing pressure is 1.5bar.

[0033] 5) Carry out cross-linking treatme...

Embodiment 2

[0037] 1) Add 1.8g of sodium alginate powder into 30ml of ultrapure water, stir for 15 hours, and wait until it is fully dissolved to obtain a sodium alginate solution.

[0038] 2) Add 3.0 g of gelatin powder into the sodium alginate solution and stir for 2 hours to obtain a gelatin / sodium alginate composite slurry.

[0039] 3) Prepare 58s bioactive glass by sol-gel method, add 2.06g of bioactive glass into the gelatin / sodium alginate composite slurry (the content of bioglass is 30%), stir for 4h until the mixture is uniform, and obtain gelatin / alginic acid Sodium / bioglass composite slurry.

[0040] 4) Pour the gelatin / sodium alginate / biological glass composite slurry obtained in step 3) into a 3d printing barrel for degassing, model in a 3d printer, and print the slurry into a bracket, wherein the size of the bracket is set to 10mm*10mm*4mm, the diameter of the printing needle is 0.41mm, the printing speed is 20mm / s, and the printing pressure is 1.0bar.

[0041] 5) Carry ou...

Embodiment 3

[0045] 1) Add 2.4g of sodium alginate powder into 30ml of ultrapure water, stir for 18 hours, and wait until it is fully dissolved to obtain a sodium alginate solution.

[0046] 2) Add 1.2 g of gelatin powder into the sodium alginate solution and stir for 4 hours to obtain a gelatin / sodium alginate composite slurry.

[0047] 3) Prepare 58s bioactive glass by sol-gel method, add 3.6g of bioactive glass to gelatin / sodium alginate composite slurry (bioglass content is 50%), stir for 6h until mixed evenly, and obtain gelatin / alginic acid Sodium / bioglass composite slurry.

[0048] 4) Pour the gelatin / sodium alginate / biological glass composite slurry obtained in step 3) into a 3d printing barrel for degassing, model in a 3d printer, and print the slurry into a bracket, wherein the size of the bracket is set to 10mm*10mm*4mm, the diameter of the printing needle is 0.41mm, the printing speed is 30mm / s, and the printing pressure is 3.0bar.

[0049] 5) Carry out cross-linking treatmen...