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Method to identify and analyze genes having modified expression in activated cells with secretory lysosomes

a technology of secretory lysosomes and genes, applied in the field of cell biology and inflammatory diseases, can solve the problems of not teaching rab37 targeting granules, and the approach does not address questions

Inactive Publication Date: 2005-03-31
BOEHRINGER INGELHEIM PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0018] e) identifying genes that are up regulated or down regulated in said experimental cells relative to said control cells.

Problems solved by technology

However, Masuda does not teach the use of Rab37 to target granules.
This approach however did not address questions such as which genes expressed in mast cells contribute to activation in response to IgE / antigen.

Method used

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  • Method to identify and analyze genes having modified expression in activated cells with secretory lysosomes
  • Method to identify and analyze genes having modified expression in activated cells with secretory lysosomes
  • Method to identify and analyze genes having modified expression in activated cells with secretory lysosomes

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Embodiment Construction

[0028] Unless defined otherwise, the scientific and technological terms and nomenclature used herein have the same meaning as commonly understood by a person of ordinary skill in the art to which this invention pertains. The procedures for cell culture and general molecular biology methods and the like are common methods used in the art. Current Protocols in Molecular Biology, John Wiley and Sons, July, 2002. [0029] Tissue—refers to one or more cells, extracts and fractions thereof. [0030] Cell—refers to cells in any form, including but not limited to, cells retained in tissue, cell clusters and individually isolated cells. [0031] Mast Cell—refers to cells of immunological origin that can be found resident in tissues throughout the body, particularly in association with structures such as blood vessels, nerves, and in proximity to surfaces in contact with the external environment. Mast cells express the FcεERI I receptor and typically release potent inflammatory mediators such as hi...

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Abstract

A method of identifying genes involved in the regulated secretion of cells having secretory lysosomes comprising the steps of: a) exposing experimental cells to an activating agent; b) preparing RNA from said experimental cells at one or more activation phases; c) measuring the level of gene expression in the cells; d) comparing the levels of gene expression of said experimental cells to the level of gene expression in control cells that have not been exposed to an activating agent; e) identifying genes that are up regulated or down regulated in said experimental cells relative to said control cells.

Description

BACKGROUND OF THE INVENTION [0001] The invention relates to the field of cell biology, and inflammatory diseases and in particular to methods for identification and analysis of genes having modified expression in activated cells with secretory lysosomes. BACKGROUND INFORMATION [0002] Cells that contain secretory lysosomes are thought to play a central role in inflammatory and allergic reactions. Numerous cell types are known to have secretory lysosomes including mast cells, goblet cells, neutrophils, natural killer cells, basophils, eosinophils, melanocytes, dendritic cells, macrophages, B cells, osteoclasts and platelets. (Griffiths, (2002) Semin. Cell Dev. Biol. 13: 279-284). All of these cell types undergo activation and release of their secretory lysosomes upon presentation of an appropriate activating agent. As a result of becoming activated these cells are known to release potent inflammatory mediators such as histamine, proteases, chemotactic factors, cytokines and metabolite...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/10
CPCC12N15/1072C12N15/1034
Inventor KABCENELL, ALISALI, JUNRAJOTTE, DANIEL
Owner BOEHRINGER INGELHEIM PHARMA INC
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