The invention relates to a method for separating and purifying
cathepsin L. The method comprises the following steps: dissociating out the
cathepsin from crude
enzyme liquid of the
cathepsin L by
thermal treatment, eliminating influence of other enzymes with
thermal instability, and then centrifuging to obtain a crude
enzyme; eliminating other cathepsin such as
cathepsin B, cathepsin H,
cathepsin S and the like, the property of which is similar with the
cathepsin L by hydrophobic gelchromatography and
ion-exchange
column chromatography, collecting components with enzymatic activity and then precipitating by 70% of
ammonium sulfate; re-dissolving the obtained precipitate by a
buffer solution and dissociating the
cathepsin L from a complex formed by the
cathepsin L and a natural inhibitor thereof at the same time, removing a low molecular weight
enzyme inhibitor through
ultrafiltration and purification and then concentrating target enzyme; and finally carrying out size-exclusion
chromatography and efficient liquid-phase separation on the obtained concentrated solution to obtain the cathepsin L. The cathepsin L (CL) obtained by the method has high purity and better activity maintenance.