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Protein markers for cardiovascular events

A cardiovascular, protein-based technology with applications in diagnostics that addresses the limited availability of molecular tests that do not take into account the likelihood of an event

Inactive Publication Date: 2010-11-17
卡瓦迪斯有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecular tests to identify patients at risk for these events are limited, and imaging is largely focused on the classical histological concept of vulnerable plaque, which does not take into account the likelihood of events

Method used

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  • Protein markers for cardiovascular events
  • Protein markers for cardiovascular events
  • Protein markers for cardiovascular events

Examples

Experimental program
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Embodiment 1

[0264] Example 1. Extraction, purification and digestion.

[0265]Plaques were ground in liquid nitrogen and approximately 500 μl of powder / plaque was used for extraction. Proteins were extracted using 500 [mu]l 40 mM Tris buffer pH 7.5 plus 1x EDTA-free protease inhibitor cocktail (Roche) and sonicated for 20 seconds on ice. After centrifugation (13000 rpm 4°C, 10 min), the supernatant (sup) was stored as Tris fraction at -80°C. The remaining precipitate was extracted with 1 ml of 75% chloroform / 25% methanol and sonicated for 20 seconds. After centrifugation (13000 rpm 4°C, 10 min), the supernatant was stored as lipid fraction and the pellet was extracted using 0.1% SDS. For this, 300 μl of 0.1% SDS were added to the pellet after 20 sec sonication and heated at 95° C. for 5 min. After centrifugation (13000 rpm 4°C, 10 min), the supernatant was used as SDS fraction on ice and stored at -80°C. Both Tris and SDS fractions were used for analysis.

[0266] The first analysis ...

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Abstract

The present invention relates to a method for predicting the risk of a subject developing a cardiovascular event comprising detecting at least one biomarker in (a sample of) the cardiovascular system from said subject, wherein said biomarker comprises at least one protein selected from the group consisting of Tumor Necrosis Factor Alpha Precursor; Lysosomal-associated Membrane Protein (1); Interleukin-5 Precursor; Interleukin-6 Precursor; C-C Motif Chemokine (2) Precursor; C-C Motif Chemokine (5) Precursor RANTES; Cathepsin L1 Precursor; Adenylate Kinase (1); Leukotriene B4 Receptor (1); Complement Factor D; Secreted Phosphoprotein (1); Small Inducible Cytokine A17 Precursor; C-X-C Motif Chemokine (10) Precursor; Tumor Necrosis Factor Ligand Superfamily Member (11)(RANKL); C-C Motif Chemokine (18) Precursor; 72 kDa Type IVCollagenase Precursor; Neutrophil Collagenase Precursor; fatty acid binding protein (4); calpain (2), (m / II) large subunit; Macrophage Migration Inhibitory Factor; Cathepsin S Precursor; Interleukin (13) Precursor; and soluble ICAM-1.

Description

field of invention [0001] The present invention relates to the field of diagnostics, including risk stratification and / or patient stratification, more particularly risk of cardiovascular events such as stroke, myocardial infarction (heart attack), cerebral hemorrhage and other major abnormalities occurring in blood vessels prognosis. In particular, the present invention relates to the classification of plaques in blood vessels, especially carotid arteries, and the prediction of the occurrence of primary and / or secondary cardiovascular adverse events. Background of the invention [0002] In Western Europe, the leading cause of death is atherosclerotic cardiovascular disease. The disease begins with atherosclerotic plaque formation in blood vessels and may progress to cardiovascular events such as cerebrovascular stroke and cardiovascular obstruction leading to myocardial infarction. These continue to be a major burden on healthcare spending, and treatment requires the most ...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N33/68
CPCG01N2800/32G01N33/6893Y10T436/143333A61P43/00A61P9/00A61P9/10
Inventor S·M·A·斯瓦格马克尔斯P·J·范德什佩克G·帕斯特坎普D·P·V·德克莱吉恩F·L·莫尔
Owner 卡瓦迪斯有限责任公司
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