68 results about "Pineapple Protease" patented technology

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The invention relates to the technical field of biological extraction, in particular to a preparation method of selenium polysaccharide in cardamine violifolia, which comprises the following steps: extracting selenium polysaccharide in cardamine violifolia by using a deep eutectic solvent as an extraction solvent and adopting an ultrasonic method, and removing protein in the selenium polysaccharide by using an isoelectric precipitation method and an enzymolysis method, thereby obtaining the selenium polysaccharide in cardamine violifolia. Removing water, enzymolysis product amino acid, inorganic selenium and other impurities by a membrane separation concentration system to obtain cardamine violifolia selenium polysaccharide; wherein the eutectic solvent comprises choline chloride and malonic acid in a molar ratio of 1: 1-1: 3, and the water content is 40-60% (mass percent); the compound enzyme is prepared from bromelain and papain in a mass ratio of 1: 1. Protein in the selenium polysaccharide is removed by adopting deep eutectic solvent ultrasonic extraction and an isoelectric precipitation method combined with an enzymolysis method, enzymolysis product amino acid, inorganic selenium and other impurities are removed by adopting an ultrafiltration membrane separation and concentration system, the structure of the polysaccharide is not damaged, and the high-purity cardamine violifolia selenium polysaccharide is obtained.

The invention discloses a microbial odor remover suitable for environment and a preparation method thereof, and relates to the technical field of daily chemical products. The preparation method comprises the following steps: adding pediococcus acidilactici and bacillus velezensis into a citric acid-disodium hydrogen phosphate buffer solution according to a ratio, standing and activating, adding bromelain, lipase, rosmarinic acid nanoemulsion, tea polyphenol and citrus polyphenol-EDTA (Ethylene Diamine Tetraacetic Acid) mixture, homogenizing, adding konjac glucomannan-chitosan gel and a trehalose-glycerol compound according to a ratio, stirring, filtering, and drying to obtain a finished product. And subpackaging in a dark place to prepare the microbial odor remover suitable for the environment. Through the synergistic effect of microorganisms, enzymes and a natural deodorization medium, a multi-stage peculiar smell elimination system is constructed, the dual functions of immediate neutralization and continuous bacteriostasis are achieved, the application scene is wide, corrosion and residues to base materials such as fabric and plastic are avoided, and the concentration of harmful gas can be more effectively controlled and pathogenic microorganisms can be inhibited in large-scale breeding application.

The invention relates to the technical field of cosmetics, and discloses a high-efficiency mild depilation cosmetic and a preparation method thereof, and the raw materials of the depilation cosmetic comprise, by weight, 8-10% of bromelain; 10%-12% of a turmeric root extract; 9%-11% of a calendula extract; 6%-9% of an aloe extract; 4%-7% of a soybean extract; 7%-10% of calcium thioglycolate; 2%-5% of L-cysteine; 1%-3% of sodium bicarbonate; 3%-6% of green tea polyphenol; 2%-4% of a chamomile extract; 1%-3% of dipotassium glycyrrhizinate; 0.9%-2.3% of a peptide component; 0.5%-1% of rosmarinic acid; 1%-3% of vitamin E; 2%-4% of gluconic acid; 4%-5% of beewax; 5%-10% of an emulsifier; according to the invention, dipotassium glycyrrhizinate and the chamomile extract form a dual anti-allergy barrier, the physical protection effect of aloe polysaccharide is combined to reduce skin irritation, and the turmeric root extract and the peptide component prolong the hair follicle resting period and reduce the hair regeneration rate, so that the safer and more effective hair removal cosmetic is prepared.

The present application relates to the technical field of polypeptide, and discloses a pearl producing Pinctada martensii with whitening and antioxidant effects, a preparation method and application thereof, wherein the pearl producing Pinctada martensii is a mixture of short peptides, the pearl producing Pinctada martensii takes pearl layer powder of Pinctada martensii as raw material, water and composite ionic liquid are added in proportion, and after physical modification under high temperature and high pressure, the pearl producing Pinctada martensii is sequentially subjected to complex degradation by bromelain and central protease, enzyme inactivation, centrifugation, separation and purification, and identification. The pearl producing Pinctada martensii prepared by the present application has the effects of whitening and free radical scavenging, and the present application provides a new type of bioactive peptide for the field of cosmetics, and simultaneously improves the application value of pearl layer powder in the fields of biological medicine and food.

The invention relates to the technical field of eggplant manufacturing, in particular to a method for manufacturing primary-color high-anthocyanin eggplants, which comprises the following steps: selecting purple eggplants, cleaning, cutting into long strips, soaking the long-strip-shaped eggplants in a honey solution, and adding citric acid into the honey solution; preparing a color protection solution, adding an enzymolysis blocking agent into the color protection solution, and pouring the eggplants into the color protection solution for later use; preparing a soaking solution, soaking the eggplants in the soaking solution for 10 minutes, taking out the eggplants, performing vacuum packaging on the eggplants to obtain the primary-color high-anthocyanin eggplants, and performing cold storage. According to the method, color protection and synergism of the eggplants are achieved through a dual-targeting mechanism, meanwhile, nutrition preservation of the eggplants is achieved, synergistic repair of cell membranes in the eggplants is achieved through combination of ficin protease and bromelain, and triple-effect removal of fungi, bacteria and biological membranes of the eggplants is achieved through combination of natamycin and subtilin.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

The present invention relates to a pig immune-enhancing feed containing natural plant extracts, belonging to the technical field of feeds. On the basis of the basic components, the feed of the present invention also adds a composite extract of plantain and selfheal, a composite extract of purslane and sweet potato vine leaves, and eucommia leaf extract. The composite extract of plantain and selfheal is obtained by successively fermenting the whole plant of plantain and the whole plant of selfheal with Lactobacillus cellobiosus and Pediococcus acidilactici, and then successively subjecting them to enzymatic hydrolysis with trypsin and nattokinase step by step; the composite extract of purslane and sweet potato vine leaves is obtained by successively fermenting the whole plant of purslane and sweet potato vine leaves with Lactobacillus cellobiosus and Bacteroides ruminicola, and then successively subjecting them to enzymatic hydrolysis with snail protease and trypsin step by step; the eucommia leaf extract is obtained by successively subjecting eucommia leaves to enzymatic hydrolysis with hemicellulase, bromelain, and nattokinase step by step. The extract contains immune-enhancing active ingredients, and when used in combination with the basic feed, it can enhance the immunity of pigs, effectively reduce diarrhea, and reduce the risk of disease.

The invention relates to an enzymolysis technology and a microwave-assisted extraction technology in a bioengineering technology, in particular to a method for extracting sika deer blood polypeptide through a microwave-assisted enzyme method and application of the sika deer blood polypeptide in anti-fatigue food. Treating the deer blood by using a composite anticoagulant containing sodium citrate, centrifuging, and freezing and thawing to obtain a lysate; carrying out microwave-assisted malic acid acidification, carrying out staged enzymolysis by using a compound enzyme composed of bromelain according to a ratio of 4: 3: 2, and introducing carbon dioxide to control the pH value; performing enzyme deactivation and centrifugal ultrafiltration to obtain a crude product, purifying the crude product through an ion exchange column and a gel column, and performing Maillard reaction modification with chitosan oligosaccharide; and finally preparing the anti-fatigue food with maltodextrin as an auxiliary material. According to the method, the yield of the sika deer blood polypeptide is greatly improved, the proportion of high-activity small molecule peptide is extremely high, the stability and bioavailability of the modified polypeptide are remarkably improved, and the bitter taste is reduced; the efficacy of the prepared anti-fatigue food is superior to that of similar products, and high-value utilization of deer blood resources is promoted.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The utility model relates to a device for removing impure protein in crude polysaccharide of traditional Chinese medicine by utilizing immobilized protease, which comprises a protein removal reactor loaded with the immobilized protease, a dissolved oxygen tester and a constant-temperature magnetic stirrer, and a dissolved oxygen electrode of the dissolved oxygen tester is connected to the protein removal reactor. The papain immobilized on the microcrystalline cellulose is used for carrying out enzymolysis reaction on impure protein in the traditional Chinese medicine crude polysaccharide, and the device has the characteristics of simplicity in operation, mild conditions and environment friendliness. The immobilized papain is limited between the two layers of screens, so that the problem of separation of the immobilized papain in the traditional Chinese medicine crude polysaccharide solution can be avoided, and the method has the characteristics of rapidness and convenience. The immobilized papain in the device can be expanded to immobilize other proteases, such as bromelain, subtilisin, protease K and the like, on cellulose according to protein types in different traditional Chinese medicine polysaccharide solutions.

The invention belongs to the field of protease extraction, and particularly relates to a method for efficiently extracting bromelain. According to the bromelain-containing mixed solution, the bromelain contained in the bromelain-containing mixed solution obtained by mixing the crushed and quick-frozen pineapples obtained by crushing and quick-freezing with the activity retention agent obtained by freeze-drying and the compound enzyme and treating is high in activity, stable in structure and high in solubility of bromelain contained in the bromelain-containing mixed solution; the adsorption protease composition containing procyanidine, lauryl sodium sulfate and zinc chloride can effectively adsorb bromelain, the auxiliary adsorption suspension capable of forming a film after drying enables bromelain molecules to be in contact with one another more easily, and the adsorption protease composition is added into the auxiliary adsorption suspension for freeze spray drying. The obtained efficient adsorption precipitant can enable the extracted bromelain to be adsorbed and precipitated more efficiently; the bromelain freeze-dried powder extracted by the method for efficiently extracting bromelain is high in enzyme activity, high in purity and strong in stability.

The application relates to the technical field of biomaterial modification, and particularly relates to a method for carrying out directional enzymolysis treatment on the surface of defatted cotton through composite plant protease, which comprises the following steps: physically pretreating the defatted cotton to enhance the surface activation degree; preparing a composite enzyme solution containing bromelain, pectinase and xylanase; contacting the pretreated defatted cotton with the composite enzyme solution to carry out directional enzymolysis under controlled conditions; controlling the enzymolysis depth by adjusting the reaction temperature and pH value in stages; after the reaction is completed, the enzyme activity is terminated through heat treatment, pH adjustment or addition of chemical reagents; finally, the treated defatted cotton is cleaned and dried to obtain a surface-modified defatted cotton product, selective directional enzymolysis of the outer layer of the defatted cotton is realized, the surface fiber diameter is reduced by 8-12%, the internal fiber structure is kept intact, the tensile strength retention rate is greater than or equal to 85%, and the problem that the depth of the existing enzymolysis technology is uncontrollable is solved.

The invention discloses a multi-step enzymolysis desensitization process for preparing low-sensitization beta-lactoglobulin peptide and application, and belongs to the technical field of food safety detection. According to the method disclosed by the invention, the anaphylaxis of the beta-lactoglobulin can be obviously reduced, and the antigen reduction rates are 62.46% (the combination of pepsin and papain) and 71.81% (the combination of bromelain and pepsin) respectively. The low-anaphylaxis beta-lactoglobulin peptide can effectively maintain the spleen index and the body temperature of an allergic model mouse at the physiological level, reduce the level of IgE (Immunoglobulin E) and improve the protective barrier of intestinal tracts. On the basis, the method can be effectively applied to development of food industry and functional food, meanwhile, technical support is provided for development of dairy product raw materials and products with higher safety, and wide industrial application prospects are shown.

The invention relates to an active pharmaceutical ingredient for repairing oral mucosa and a preparation method of the active pharmaceutical ingredient, and belongs to the technical field of biological medicine, and the active pharmaceutical ingredient comprises an amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance, a dendrobium nobile callus extract, sorbitol, menthol, a gel matrix and the like. The amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance is obtained by entrapment of a compound of amanita aurantiaca mycelium active peptide and myrobalan polyphenol through a sodium alginate-dopamine grafted copolymer; the amanita aurantiaca mycelium active peptide is obtained by performing common enzymolysis on the mycelium of amanita aurantiaca through bromelain and bacillus subtilis protease. The dendrobium nobile callus extract is obtained by inducing slices of dendrobium nobile seedling leaves with 2, 4-D and 6-BA, extracting and purifying. The gel matrix is prepared from modified konjac glucomannan and carboxymethyl chitosan. All the components form an anti-inflammatory, proliferative and remodeled closed-loop repair system, and the anti-degradation stability and the adhesion long-term effect are improved.

The invention belongs to the technical field of comprehensive utilization of pineapple peels, and discloses a comprehensive utilization process of pineapple peels, which specifically comprises the following steps: (1) crushing and squeezing fresh pineapple peels to obtain pineapple peel juice and pineapple residues; (2) filtering the pineapple peel juice through a vibrating screen to obtain filtered juice; fermenting the pineapple residues to obtain a fermented feed; (3) freezing the filtered juice, clarifying the frozen filtered juice, and adding a coagulant release agent during clarification to obtain clear juice; (4) performing ultrafiltration treatment on the clear juice to obtain clear liquid, and extracting bromelain; and (5) preparing the obtained clear liquid into a functional product. According to the method, waste is turned into wealth, the pineapple peel is produced into bromelain, yeast, yeast extract, pineapple concentrated juice, pineapple residue probiotic feed and other high-quality products with high additional value, environmental protection is achieved, income is created, and the method is an extremely good innovative industry.

The invention relates to the technical field of cosmetics, in particular to a preparation method of a supramolecular oleic acid control enzyme composition and application of the supramolecular oleic acid control enzyme composition in cosmetics. The invention relates to a preparation method of a supramolecular controlled oleic enzyme composition. The preparation method comprises the following steps: preparation of a supramolecular NaDES solvent: carrying out a reaction on mandelic acid, betaine and capryloyl glycine to obtain the supramolecular NaDES solvent; preparation of a supramolecular acid enzyme composition: reacting the papain and bromelain compound with a supramolecular NaDES solvent to obtain the supramolecular acid enzyme composition; mandelic acid, betaine and capryloyl glycine are prepared into a supramolecular deep eutectic solvent and a protease compound, and the protease compound is immobilized in the supramolecular deep eutectic solvent, so that the method has the characteristics of simplicity in operation, good solubility, good stability and the like; the finally obtained supramolecular oleate-controlling enzyme composition can realize the combination of acid, amino acid derivatives and enzyme, and achieves the effects of synergistically removing waste cutin and blocked pores of skin, controlling oil, quickly improving the texture and appearance of skin and promoting metabolism of skin.

The invention discloses a gene LpOryzain alpha for delaying senescence of perennial ryegrass leaves and application, and belongs to the technical field of plant genetic engineering. According to the invention, a cysteine protease LpOryzain alpha is identified in perennial ryegrass, and the nucleotide sequence of the gene CDS is as shown in SEQ ID NO. 9. The expression quantity of the LpOryzain alpha in the perennial ryegrass is higher along with the aging of leaves. Furthermore, in overexpression of the LpOryzain alpha in arabidopsis thaliana and perennial ryegrass, it is found that the LpOryzain alpha can delay the senescence degree of leaves. Therefore, the perennial ryegrass leaf senescence gene LpOryzain alpha has the function of delaying plant leaf senescence, and an important reference gene and a theoretical basis are provided for improving the quality of turfgrass and pasture.

The invention discloses a preparation method of a natural plant-based kitchen heavy oil stain cleaning agent, and relates to the technical field of daily chemical industry, and the preparation method comprises the following steps: selecting palm fruits, coconut meat and pineapple stems as raw materials, crushing, sieving, adding deionized water, carrying out ultrasonic-microwave synergistic extraction to obtain a mixed crude extract, filtering, and drying to obtain the natural plant-based kitchen heavy oil stain cleaning agent. The method disclosed by the invention has the advantages that the vegetable fatty acid and the bromelain are obtained by adopting an ultrasonic-microwave synergistic extraction technology, the enzyme catalysis efficiency is improved by combining directed evolution mutagenesis, and then a nano-micelle structure capable of permeating oil stains is formed through biomimetic modification of a cell membrane; meanwhile, the adaptability of a cleaning environment is enhanced by utilizing a pH self-adaptive adjusting system, the preparation method accurately breaks through an oxidative polymerization structure of aged oil stains through the synergistic effect of multiple components, the defect that an existing cleaning agent is insufficient in decomposition capacity on stubborn oil stains is overcome, and efficient emulsification, decomposition and rapid cleaning of kitchen heavy oil stains are achieved.

The present invention provides a probe compound for labeling a cysteine protease based on activity. The compound targets the protease through a specific targeting element. The compound also includes a detectable element, such as a fluorescent label, a radioactive label, or a chelator. In some cases, the compound also includes a quenching element that is released upon reaction with the protease. The present invention also provides compositions comprising the compound, as well as methods of using the compound, such as labeling a protease in an animal and visualizing a tumor in an animal.

The present application relates to a kind of active pharmaceutical ingredients of repairing oral mucosa and preparation method thereof, belong to biological medicine technical field, and drug includes orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package, dendrobium nobile callus extract, sorbitol, menthol, gel matrix etc.Orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package is the complex of orange cover gonggao mycelium active peptide and Terminalia polyphenol by sodium alginate-dopamine graft copolymer package;Orange cover gonggao mycelium active peptide is the mycelium of orange cover gonggao mushroom by bromelain and bacillus subtilis protease common enzymolysis obtains.Golden lace dendrobium callus extract is the section of golden lace dendrobium seedling leaf by 2,4-D and 6-BA induction, extraction, purification and obtains.Gel matrix is prepared by modified konjac glucomannan and carboxymethyl chitosan.Each component forms anti-inflammatory, proliferation and remodeling closed-loop repair system, and improves the stability of anti-degradation and adheres long-acting.

The invention relates to a multi-effect synergistic pharmaceutical composition for bone repair and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises five-black chicken yolk peptide, tegillarca granosa peptide, epimedium extract and rhizoma drynariae extract. The five-black chicken egg yolk peptide is obtained by carrying out enzymolysis on five-black chicken egg yolk freeze-dried powder by lipase and trypsin, then carrying out enzymolysis by ficin and bromelain, and then sequentially purifying by a macroporous adsorption resin column and a phenyl agarose gel column. The tegillarca granosa peptide is obtained by fermenting tegillarca granosa meat through bacillus marinus; the herba epimedii extract is obtained by purifying an upper phase extracted from herba epimedii powder through an aqueous two-phase system through a sephadex chromatographic column; the rhizoma drynariae extract is obtained by performing enzymolysis on rhizoma drynariae powder with cellulase, extracting with a sodium dioctyl sulfosuccinate-isooctane reverse micelle solution, taking an upper organic phase, breaking micelles, taking a lower water phase and performing ultrafiltration. The four components are matched according to a specific proportion to realize multi-effect regulation of promoting formation, inhibiting bone resorption and regulating hormone.

The present invention discloses a scomber japonicus dark muscle enzymolysis antibacterial peptide, its preparation method and use. The characteristics are that the amino acid sequence of the antibacterial peptide is Arg-Ser-Cys-His and / or Lys-Ser-Cys-Gly. The steps of its preparation method are as follows: Add deionized water with a mass 2-4 times that of the fish meat to the defatted scomber japonicus dark muscle and adjust the pH to 7-11, then add trypsin and bacillus licheniformis protease for enzymolysis, then incubate to inactivate the enzyme and centrifuge, and take the supernatant to obtain the crude enzymolysis solution; Add C-terminal endo-double enzyme cysteine protease ArgC and serine protease LysC to the crude enzymolysis solution for enzymolysis, incubate to inactivate the enzyme and centrifuge to obtain the upper enzymolysis clear solution, then filter it with an ultrafiltration system to obtain an enzymolysis solution of 1-3 kDa, collect the filtrate and freeze-dry it, and the powder is the scomber japonicus dark muscle enzymolysis antibacterial peptide. The advantages are high extraction efficiency and significant inhibitory effects on both Gram-positive bacteria and Gram-negative bacteria.

The invention provides a preparation method of giant salamander peptide, and belongs to the technical field of bioactive peptide preparation. The quality of the giant salamander peptide is greatly improved through low-temperature stress treatment before slaughtering, variable-pressure boiling, beta-cyclodextrin and sequential addition of neutral protease, papain, bromelain and the like, the finally obtained giant salamander peptide is high in small molecule peptide proportion and low in bitter taste, and the biological activity is remarkably superior to that of a product prepared by a traditional method; the method is very suitable for developing high-end functional food and health care products.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".

The invention provides a micromolecular albumin peptide capable of promoting growth and development as well as a preparation method and application thereof, and belongs to the technical field of biology. According to the invention, bromelain, alkaline protease and flavourzyme are creatively adopted to carry out step-by-step mixed enzymolysis on egg white. Through scientific compounding and synergistic effects of various enzymes, the hydrolysis efficiency of the micromolecule albumin peptide is greatly improved, the taste of the micromolecule albumin peptide is improved, compared with similar products on the market, the micromolecule albumin peptide has the flavor advantage, and the problems of low yield, obvious bitter taste and the like in a traditional process are effectively solved. The average relative molecular weight of the small molecule peptide prepared by the process is stabilized in a range of 200-1000Da, the property is more stable, and the small molecule peptide has the characteristic of being extremely easily absorbed by a human body. Experimental results prove that the micromolecular protein peptide also has a remarkable effect of promoting growth and development.

Provided herein are methods and compositions for reducing and preventing pain. In some embodiments, the methods include administration of a composition to an individual experiencing pain, the composition including one or more of Incarvillea sinensis, Salix alba, Tanacetum parthenium, Petasites hybridus, papain, and bromelain.

The invention relates to medicinal oral liquid of glycosaminoglycan and a preparation method, and belongs to the technical field of biological medicines.The oral liquid adopts glycosaminoglycan-rich collagen peptide, cod collagen oligopeptide powder, skipjack elastin peptide-Y, fish maw oligopeptide powder, fish collagen tripeptide, apple concentrated juice, white birch juice and the like as effective active ingredients, the apple concentrated juice is obtained by sequentially carrying out step-by-step enzymolysis on green apples through bromelain, ficin and trypsin; the white birch juice is obtained by carrying out anaerobic fermentation on white birch juice through lactobacillus brevis and lactobacillus cellosus. All the components are matched for use, so that the food has very good effects of reducing blood fat, increasing bone mineral density, improving immunity, resisting fatigue and the like, and the action effect is rapid and effective.

The present invention relates to the technical field related to proteolysis technology, and discloses an industrial preparation process and application of a hydrolyzed egg yolk powder that can promote tibial growth. This preparation technology has high repeatability and can achieve industrial production. By using a high-pressure enzymolysis tank and through high-temperature and high-pressure treatment, volatile odor substances in egg yolk protein are evaporated, improving the flavor and taste of the product, and opening the protein structure to expose the protease hydrolysis sites, making it easier to bind with proteases. Proteases derived from papaya, pineapple, and Bacillus subtilis are selected for composite directional enzymolysis to improve the enzymolysis efficiency, shorten the enzymolysis time, and increase the protein yield; among them, papain and alkaline protease derived from Bacillus subtilis are endopeptidases, which can more effectively hydrolyze from the inside of the protein with an opened spatial structure to obtain more peptide components, and then through the end-cutting action of bromelain and flavorzyme, the proportion of hydrophobic amino acids at the peptide ends is reduced, improving the product flavor.