8 results about "Pineapple Protease" patented technology

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

Provided herein are methods and compositions for reducing and preventing pain. In some embodiments, the methods include administration of a composition to an individual experiencing pain, the composition including one or more of Incarvillea sinensis, Salix alba, Tanacetum parthenium, Petasites hybridus, papain, and bromelain.

This invention discloses a chicken embryo fibroblast, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method of the chicken embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the muscle tissue of the chicken embryos; S2, mixing the muscle tissue with a plant-derived compound digestion solution, and sequentially performing digestion, purification, and primary culture treatment using a serum-free special culture medium to obtain primary chicken embryo fibroblasts; wherein, the plant-derived compound digestion solution includes bromelain and papain. This invention, using a plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability, cell yield, and cell purity of the cells, reducing the risk of exogenous viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in viral culture.

The application discloses a preparation method of balsam pear seed peptides by enzymolysis, and belongs to the technical field of peptide preparation. The preparation method comprises the following steps: after balsam pear seeds are crushed, water is added for soaking treatment to obtain material A; cellulase and pectinase are added to the material A for pretreatment, enzyme inactivation is performed, and a primary enzymolysis liquid is obtained; compound protease is added to the primary enzymolysis liquid for step-by-step enzymolysis, enzyme inactivation is performed, solid-liquid separation is performed, and an enzymolysis liquid is obtained; the enzymolysis liquid is dried to obtain polypeptide powder; the polypeptide powder is extracted by ethanol, filtered, and dried to obtain the balsam pear seed peptides; and the compound protease in step (3) is bromelain, pepsin and carboxypeptidase B. Compared with the prior art, the balsam pear seed peptides prepared by the application have better alpha-glucosidase inhibiting capacity, are close to chemical hypoglycemic drugs, can be used as natural alpha-glucosidase inhibitor raw materials, and have lower side effects.

The invention relates generally to cleavable polypeptides, method of producing a cleavable moiety (CM) containing polypeptide by culturing a cell under conditions that lead to expression of the polypeptide, methods of manufacturing a cleavable moiety (CM) containing polypeptide by culturing a cell comprising a nucleic acid construct that encodes the cleavable polypeptide to express the cleavable polypeptide, and recovering the cleavable polypeptide, nucleic acids encoding the cleavable polypeptides, and vectors including the nucleic acids encoding the cleavable polypeptides.

A processing method for long-shelf-life whole egg liquid includes the following steps: S1, raw material pretreatment; S2, primary enzymatic hydrolysis of whole egg liquid; S3, secondary enzymatic hydrolysis of whole egg liquid; S4, degassing and cooling; S5, filling; and S6, ultra-high pressure sterilization. This invention employs a synergistic process of "stepwise enzymatic hydrolysis + vacuum degassing + ultra-high pressure sterilization." Phospholipase hydrolysis of the whole egg liquid improves its stability and generates antibacterial components. Subsequent bromelain-transglutaminase complex enzymatic hydrolysis produces antimicrobial peptides through proteolysis while simultaneously preventing excessive protein degradation and inhibiting microbial growth through transglutaminase. Combined with vacuum degassing to reduce oxidizing agents and the highly efficient antibacterial effect of ultra-high pressure sterilization, the finished whole egg liquid can achieve a shelf life of over 37 days under 4°C refrigeration conditions, far exceeding the traditional 15-21 days for whole egg liquid, thus solving the spoilage problem during storage and distribution.

The invention relates generally to cleavable polypeptides, method of producing a cleavable moiety (CM) containing polypeptide by culturing a cell under conditions that lead to expression of the polypeptide, methods of manufacturing a cleavable moiety (CM) containing polypeptide by culturing a cell comprising a nucleic acid construct that encodes the cleavable polypeptide to express the cleavable polypeptide, and recovering the cleavable polypeptide, nucleic acids encoding the cleavable polypeptides, and vectors including the nucleic acids encoding the cleavable polypeptides.

This invention discloses a synergistic extraction process for highly active white jade snail enzymes, belonging to the field of bioenzyme extraction technology. Using the soft tissue and foot gland mucus of high-quality white jade snails as composite raw materials, an integrated technical route of "low-temperature and mild pretreatment – ​​ultrasonic-microwave synergistic cell disruption – directional hydrolysis of composite enzymes – gradient membrane separation and purification – vacuum freeze-drying" is employed. The core process parameters are: synergistic action of 20kHz ultrasound and 2450MHz microwaves at a power ratio of 2:1, combined with a composite enzyme system composed of papain and bromelain at a mass ratio of 1:0.8, achieving a comprehensive enzyme extraction rate of over 94.2%; deep pre-freezing at -60℃ and vacuum sublimation drying, resulting in an enzyme activity retention rate ≥96.5%. The finished product is a off-white powder with water solubility ≥99.3%, protease specific activity ≥1800U / mg, skin fibroblast proliferation promotion rate ≥45%, and no organic solvent residue, making it suitable for high-end cosmetics, biocatalysis, and functional health products.