47 results about "Pineapple Protease" patented technology

The invention relates to the technical field of biological extraction, in particular to a preparation method of selenium polysaccharide in cardamine violifolia, which comprises the following steps: extracting selenium polysaccharide in cardamine violifolia by using a deep eutectic solvent as an extraction solvent and adopting an ultrasonic method, and removing protein in the selenium polysaccharide by using an isoelectric precipitation method and an enzymolysis method, thereby obtaining the selenium polysaccharide in cardamine violifolia. Removing water, enzymolysis product amino acid, inorganic selenium and other impurities by a membrane separation concentration system to obtain cardamine violifolia selenium polysaccharide; wherein the eutectic solvent comprises choline chloride and malonic acid in a molar ratio of 1: 1-1: 3, and the water content is 40-60% (mass percent); the compound enzyme is prepared from bromelain and papain in a mass ratio of 1: 1. Protein in the selenium polysaccharide is removed by adopting deep eutectic solvent ultrasonic extraction and an isoelectric precipitation method combined with an enzymolysis method, enzymolysis product amino acid, inorganic selenium and other impurities are removed by adopting an ultrafiltration membrane separation and concentration system, the structure of the polysaccharide is not damaged, and the high-purity cardamine violifolia selenium polysaccharide is obtained.

The invention discloses a microbial odor remover suitable for environment and a preparation method thereof, and relates to the technical field of daily chemical products. The preparation method comprises the following steps: adding pediococcus acidilactici and bacillus velezensis into a citric acid-disodium hydrogen phosphate buffer solution according to a ratio, standing and activating, adding bromelain, lipase, rosmarinic acid nanoemulsion, tea polyphenol and citrus polyphenol-EDTA (Ethylene Diamine Tetraacetic Acid) mixture, homogenizing, adding konjac glucomannan-chitosan gel and a trehalose-glycerol compound according to a ratio, stirring, filtering, and drying to obtain a finished product. And subpackaging in a dark place to prepare the microbial odor remover suitable for the environment. Through the synergistic effect of microorganisms, enzymes and a natural deodorization medium, a multi-stage peculiar smell elimination system is constructed, the dual functions of immediate neutralization and continuous bacteriostasis are achieved, the application scene is wide, corrosion and residues to base materials such as fabric and plastic are avoided, and the concentration of harmful gas can be more effectively controlled and pathogenic microorganisms can be inhibited in large-scale breeding application.

The present application relates to the technical field of polypeptide, and discloses a pearl producing Pinctada martensii with whitening and antioxidant effects, a preparation method and application thereof, wherein the pearl producing Pinctada martensii is a mixture of short peptides, the pearl producing Pinctada martensii takes pearl layer powder of Pinctada martensii as raw material, water and composite ionic liquid are added in proportion, and after physical modification under high temperature and high pressure, the pearl producing Pinctada martensii is sequentially subjected to complex degradation by bromelain and central protease, enzyme inactivation, centrifugation, separation and purification, and identification. The pearl producing Pinctada martensii prepared by the present application has the effects of whitening and free radical scavenging, and the present application provides a new type of bioactive peptide for the field of cosmetics, and simultaneously improves the application value of pearl layer powder in the fields of biological medicine and food.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

The application relates to the field of biological medicine, and particularly discloses application of a sophora japonica extract in preparation of a product for improving the reproductive performance of ewes. The sophora japonica extract can effectively inhibit pyroptosis of ovarian granulosa cells by down-regulating expression of cysteine protease B, further reverses follicle atresia and ovulation disorders caused by pyroptosis, and improves the reproductive performance; animal experiments show that the sophora japonica extract can optimize the follicle development structure of ewes, inhibit overdevelopment of large follicles, and significantly improve the ovulation efficiency, thereby providing a new scheme for inhibiting pyroptosis of ovarian granulosa cells and improving the reproductive performance of livestock.

The utility model relates to a device for removing impure protein in crude polysaccharide of traditional Chinese medicine by utilizing immobilized protease, which comprises a protein removal reactor loaded with the immobilized protease, a dissolved oxygen tester and a constant-temperature magnetic stirrer, and a dissolved oxygen electrode of the dissolved oxygen tester is connected to the protein removal reactor. The papain immobilized on the microcrystalline cellulose is used for carrying out enzymolysis reaction on impure protein in the traditional Chinese medicine crude polysaccharide, and the device has the characteristics of simplicity in operation, mild conditions and environment friendliness. The immobilized papain is limited between the two layers of screens, so that the problem of separation of the immobilized papain in the traditional Chinese medicine crude polysaccharide solution can be avoided, and the method has the characteristics of rapidness and convenience. The immobilized papain in the device can be expanded to immobilize other proteases, such as bromelain, subtilisin, protease K and the like, on cellulose according to protein types in different traditional Chinese medicine polysaccharide solutions.

The application relates to the technical field of biomaterial modification, and particularly relates to a method for carrying out directional enzymolysis treatment on the surface of defatted cotton through composite plant protease, which comprises the following steps: physically pretreating the defatted cotton to enhance the surface activation degree; preparing a composite enzyme solution containing bromelain, pectinase and xylanase; contacting the pretreated defatted cotton with the composite enzyme solution to carry out directional enzymolysis under controlled conditions; controlling the enzymolysis depth by adjusting the reaction temperature and pH value in stages; after the reaction is completed, the enzyme activity is terminated through heat treatment, pH adjustment or addition of chemical reagents; finally, the treated defatted cotton is cleaned and dried to obtain a surface-modified defatted cotton product, selective directional enzymolysis of the outer layer of the defatted cotton is realized, the surface fiber diameter is reduced by 8-12%, the internal fiber structure is kept intact, the tensile strength retention rate is greater than or equal to 85%, and the problem that the depth of the existing enzymolysis technology is uncontrollable is solved.

The invention discloses a multi-step enzymolysis desensitization process for preparing low-sensitization beta-lactoglobulin peptide and application, and belongs to the technical field of food safety detection. According to the method disclosed by the invention, the anaphylaxis of the beta-lactoglobulin can be obviously reduced, and the antigen reduction rates are 62.46% (the combination of pepsin and papain) and 71.81% (the combination of bromelain and pepsin) respectively. The low-anaphylaxis beta-lactoglobulin peptide can effectively maintain the spleen index and the body temperature of an allergic model mouse at the physiological level, reduce the level of IgE (Immunoglobulin E) and improve the protective barrier of intestinal tracts. On the basis, the method can be effectively applied to development of food industry and functional food, meanwhile, technical support is provided for development of dairy product raw materials and products with higher safety, and wide industrial application prospects are shown.

The invention relates to an active pharmaceutical ingredient for repairing oral mucosa and a preparation method of the active pharmaceutical ingredient, and belongs to the technical field of biological medicine, and the active pharmaceutical ingredient comprises an amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance, a dendrobium nobile callus extract, sorbitol, menthol, a gel matrix and the like. The amanita aurantiaca mycelium active peptide-myrobalan polyphenol composite entrapment substance is obtained by entrapment of a compound of amanita aurantiaca mycelium active peptide and myrobalan polyphenol through a sodium alginate-dopamine grafted copolymer; the amanita aurantiaca mycelium active peptide is obtained by performing common enzymolysis on the mycelium of amanita aurantiaca through bromelain and bacillus subtilis protease. The dendrobium nobile callus extract is obtained by inducing slices of dendrobium nobile seedling leaves with 2, 4-D and 6-BA, extracting and purifying. The gel matrix is prepared from modified konjac glucomannan and carboxymethyl chitosan. All the components form an anti-inflammatory, proliferative and remodeled closed-loop repair system, and the anti-degradation stability and the adhesion long-term effect are improved.

The invention belongs to the technical field of comprehensive utilization of pineapple peels, and discloses a comprehensive utilization process of pineapple peels, which specifically comprises the following steps: (1) crushing and squeezing fresh pineapple peels to obtain pineapple peel juice and pineapple residues; (2) filtering the pineapple peel juice through a vibrating screen to obtain filtered juice; fermenting the pineapple residues to obtain a fermented feed; (3) freezing the filtered juice, clarifying the frozen filtered juice, and adding a coagulant release agent during clarification to obtain clear juice; (4) performing ultrafiltration treatment on the clear juice to obtain clear liquid, and extracting bromelain; and (5) preparing the obtained clear liquid into a functional product. According to the method, waste is turned into wealth, the pineapple peel is produced into bromelain, yeast, yeast extract, pineapple concentrated juice, pineapple residue probiotic feed and other high-quality products with high additional value, environmental protection is achieved, income is created, and the method is an extremely good innovative industry.

The invention relates to the technical field of cosmetics, in particular to a preparation method of a supramolecular oleic acid control enzyme composition and application of the supramolecular oleic acid control enzyme composition in cosmetics. The invention relates to a preparation method of a supramolecular controlled oleic enzyme composition. The preparation method comprises the following steps: preparation of a supramolecular NaDES solvent: carrying out a reaction on mandelic acid, betaine and capryloyl glycine to obtain the supramolecular NaDES solvent; preparation of a supramolecular acid enzyme composition: reacting the papain and bromelain compound with a supramolecular NaDES solvent to obtain the supramolecular acid enzyme composition; mandelic acid, betaine and capryloyl glycine are prepared into a supramolecular deep eutectic solvent and a protease compound, and the protease compound is immobilized in the supramolecular deep eutectic solvent, so that the method has the characteristics of simplicity in operation, good solubility, good stability and the like; the finally obtained supramolecular oleate-controlling enzyme composition can realize the combination of acid, amino acid derivatives and enzyme, and achieves the effects of synergistically removing waste cutin and blocked pores of skin, controlling oil, quickly improving the texture and appearance of skin and promoting metabolism of skin.

The invention discloses a preparation method of a natural plant-based kitchen heavy oil stain cleaning agent, and relates to the technical field of daily chemical industry, and the preparation method comprises the following steps: selecting palm fruits, coconut meat and pineapple stems as raw materials, crushing, sieving, adding deionized water, carrying out ultrasonic-microwave synergistic extraction to obtain a mixed crude extract, filtering, and drying to obtain the natural plant-based kitchen heavy oil stain cleaning agent. The method disclosed by the invention has the advantages that the vegetable fatty acid and the bromelain are obtained by adopting an ultrasonic-microwave synergistic extraction technology, the enzyme catalysis efficiency is improved by combining directed evolution mutagenesis, and then a nano-micelle structure capable of permeating oil stains is formed through biomimetic modification of a cell membrane; meanwhile, the adaptability of a cleaning environment is enhanced by utilizing a pH self-adaptive adjusting system, the preparation method accurately breaks through an oxidative polymerization structure of aged oil stains through the synergistic effect of multiple components, the defect that an existing cleaning agent is insufficient in decomposition capacity on stubborn oil stains is overcome, and efficient emulsification, decomposition and rapid cleaning of kitchen heavy oil stains are achieved.

The present invention provides a probe compound for labeling a cysteine protease based on activity. The compound targets the protease through a specific targeting element. The compound also includes a detectable element, such as a fluorescent label, a radioactive label, or a chelator. In some cases, the compound also includes a quenching element that is released upon reaction with the protease. The present invention also provides compositions comprising the compound, as well as methods of using the compound, such as labeling a protease in an animal and visualizing a tumor in an animal.

The present application relates to a kind of active pharmaceutical ingredients of repairing oral mucosa and preparation method thereof, belong to biological medicine technical field, and drug includes orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package, dendrobium nobile callus extract, sorbitol, menthol, gel matrix etc.Orange cover gonggao mycelium active peptide- Terminalia polyphenol composite package is the complex of orange cover gonggao mycelium active peptide and Terminalia polyphenol by sodium alginate-dopamine graft copolymer package;Orange cover gonggao mycelium active peptide is the mycelium of orange cover gonggao mushroom by bromelain and bacillus subtilis protease common enzymolysis obtains.Golden lace dendrobium callus extract is the section of golden lace dendrobium seedling leaf by 2,4-D and 6-BA induction, extraction, purification and obtains.Gel matrix is prepared by modified konjac glucomannan and carboxymethyl chitosan.Each component forms anti-inflammatory, proliferation and remodeling closed-loop repair system, and improves the stability of anti-degradation and adheres long-acting.

The invention relates to a multi-effect synergistic pharmaceutical composition for bone repair and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises five-black chicken yolk peptide, tegillarca granosa peptide, epimedium extract and rhizoma drynariae extract. The five-black chicken egg yolk peptide is obtained by carrying out enzymolysis on five-black chicken egg yolk freeze-dried powder by lipase and trypsin, then carrying out enzymolysis by ficin and bromelain, and then sequentially purifying by a macroporous adsorption resin column and a phenyl agarose gel column. The tegillarca granosa peptide is obtained by fermenting tegillarca granosa meat through bacillus marinus; the herba epimedii extract is obtained by purifying an upper phase extracted from herba epimedii powder through an aqueous two-phase system through a sephadex chromatographic column; the rhizoma drynariae extract is obtained by performing enzymolysis on rhizoma drynariae powder with cellulase, extracting with a sodium dioctyl sulfosuccinate-isooctane reverse micelle solution, taking an upper organic phase, breaking micelles, taking a lower water phase and performing ultrafiltration. The four components are matched according to a specific proportion to realize multi-effect regulation of promoting formation, inhibiting bone resorption and regulating hormone.

The invention provides a preparation method of giant salamander peptide, and belongs to the technical field of bioactive peptide preparation. The quality of the giant salamander peptide is greatly improved through low-temperature stress treatment before slaughtering, variable-pressure boiling, beta-cyclodextrin and sequential addition of neutral protease, papain, bromelain and the like, the finally obtained giant salamander peptide is high in small molecule peptide proportion and low in bitter taste, and the biological activity is remarkably superior to that of a product prepared by a traditional method; the method is very suitable for developing high-end functional food and health care products.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".

The invention provides a micromolecular albumin peptide capable of promoting growth and development as well as a preparation method and application thereof, and belongs to the technical field of biology. According to the invention, bromelain, alkaline protease and flavourzyme are creatively adopted to carry out step-by-step mixed enzymolysis on egg white. Through scientific compounding and synergistic effects of various enzymes, the hydrolysis efficiency of the micromolecule albumin peptide is greatly improved, the taste of the micromolecule albumin peptide is improved, compared with similar products on the market, the micromolecule albumin peptide has the flavor advantage, and the problems of low yield, obvious bitter taste and the like in a traditional process are effectively solved. The average relative molecular weight of the small molecule peptide prepared by the process is stabilized in a range of 200-1000Da, the property is more stable, and the small molecule peptide has the characteristic of being extremely easily absorbed by a human body. Experimental results prove that the micromolecular protein peptide also has a remarkable effect of promoting growth and development.

Provided herein are methods and compositions for reducing and preventing pain. In some embodiments, the methods include administration of a composition to an individual experiencing pain, the composition including one or more of Incarvillea sinensis, Salix alba, Tanacetum parthenium, Petasites hybridus, papain, and bromelain.

The invention relates to medicinal oral liquid of glycosaminoglycan and a preparation method, and belongs to the technical field of biological medicines.The oral liquid adopts glycosaminoglycan-rich collagen peptide, cod collagen oligopeptide powder, skipjack elastin peptide-Y, fish maw oligopeptide powder, fish collagen tripeptide, apple concentrated juice, white birch juice and the like as effective active ingredients, the apple concentrated juice is obtained by sequentially carrying out step-by-step enzymolysis on green apples through bromelain, ficin and trypsin; the white birch juice is obtained by carrying out anaerobic fermentation on white birch juice through lactobacillus brevis and lactobacillus cellosus. All the components are matched for use, so that the food has very good effects of reducing blood fat, increasing bone mineral density, improving immunity, resisting fatigue and the like, and the action effect is rapid and effective.

A recombinant TEV protease, a preparation method therefor, and use thereof, relating to the technical field of biology. Specifically provided are four recombinant TEV proteases derived from a cysteine protease of tobacco etch virus. In each of the four recombinant TEV proteases, the N-terminus is fused with an 8His-CL7-GGS tag, and the C-terminus is fused with a polyarginine tag. Compared to a wild-type TEV protease, the provided four recombinant TEV proteases not only can overcome defects such as self-cleavage, poor solubility, and low activity during expression and purification of the wild-type TEV protease, but also have improved protein yield 5-6 times higher than that of the wild-type TEV enzyme, and enzyme activity 10-20 times higher than that of the wild-type TEV protease. In addition, the engineered TEV protease mutants also possess higher thermal stability with a Tm value 10-30 °C higher than that of the wild-type TEV protease, exhibit better protein stability, have broader application conditions, and are more suitable for large-scale production and industrial use.

The invention relates to the technical field of food processing, and particularly discloses high-nutrition pineapple noodles and a preparation method thereof.The high-nutrition pineapple noodles comprise enzymolysis wheat flour, high-gluten wheat flour, modified pineapple residues, pineapple soluble dietary fiber dry powder and blended pineapple juice, the preparation method comprises the steps that S1, bromelain is used for conducting enzymolysis on the high-gluten wheat flour to obtain the enzymolysis wheat flour, and the enzymolysis wheat flour is used for conducting enzymolysis on the pineapple soluble dietary fiber dry powder; the method comprises the following steps: S1, squeezing fresh pineapples into juice, adding edible salt and dietary alkali, and blending, S3, synchronously extracting pineapple soluble dietary fibers from wet pineapple residues, and preparing modified pineapple residues, and S4, carrying out dry mixing on all solid raw materials, adding a pre-hydrated dietary fiber glue solution and blended pineapple juice, and carrying out dough kneading, dough standing, tabletting and slitting, and drying so as to obtain a finished product. The activity of bromelain is inhibited through the edible salt and the dietary alkali, and gluten protein is protected; the enzymolysis wheat flour provides small molecule peptides, the nutrition absorption efficiency is improved, the modified pineapple residues improve the processing performance of the dough, and finally the nutrition enhancement and the processing performance improvement of the product are achieved.

The invention relates generally to cleavable polypeptides, method of producing a cleavable moiety (CM) containing polypeptide by culturing a cell under conditions that lead to expression of the polypeptide, methods of manufacturing a cleavable moiety (CM) containing polypeptide by culturing a cell comprising a nucleic acid construct that encodes the cleavable polypeptide to express the cleavable polypeptide, and recovering the cleavable polypeptide, nucleic acids encoding the cleavable polypeptides, and vectors including the nucleic acids encoding the cleavable polypeptides.

The present invention relates to IdeS protein variants having IgG cleaving cysteine protease activity and having at least 90%, at least 95%, at least 98%, at least 99% sequence identity to SEQ ID No.2 and (a) having glutamic acid (E) at a position corresponding to position 226 of SEQ ID No.1; or (b) arginine (R) at the position 167 corresponding to SEQ ID No.1 and glutamic acid (E) at the position 226 corresponding to SEQ ID No.1. The present invention also relates to a nucleic acid encoding said IdeS protein variant, a vector comprising said nucleic acid, and the use of said IdeS protein variant for the treatment or prevention of a disease or disorder associated with IgG; or as a tool enzyme for biotechnology in vitro.

The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG and in vitro generation of F(ab′)2 fragments.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a corn chromosome 4 white spot disease resistance related molecular marker and application, specifically, the application provides a corn white spot disease resistance related molecular marker, the molecular marker gene is a Zm00001eb168510 gene and / or a sequence as shown in SEQ ID NO:1, a CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO:2, through years of experiments in multiple points, the application is co-located to a white spot disease resistance QTL qMWS4-4 interval and a resistance SNP:4-14146725 and an associated gene Zm00001eb168510 on a corn chromosome 4, the SNP site can be located in different environments through QTL linkage analysis and GWAS correlation analysis, and can explain 15.14% of the phenotype variation; the associated candidate functional gene mainly encodes a cysteine protease superfamily protein, the cysteine protease superfamily contains a deubiquitinating enzyme, and plays an important role in plant development and adversity response.

The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for the prevention or treatment of diseases and conditions mediated by IgG, and methods for the analysis of IgG and in vitro generation of F(ab′)2 fragments.

The invention relates to the technical field of anti-fatigue peptide preparation, and discloses an anti-fatigue peptide preparation method, which comprises: 1, raw material pretreatment: selecting fresh deep sea fish skin, washing, dicing, carrying out freeze drying, and carrying out superfine grinding under nitrogen protection to obtain fish skin micro powder; step 2, preparation of a composite enzymatic hydrolysate: mixing trypsin, papain and bromelain according to a ratio, and adding the mixture into a phosphoric acid buffer solution to prepare the composite enzymatic hydrolysate; a compound enzyme and ultrasonic wave synergistic enzymolysis process is adopted, the synergistic effect of the compound enzyme is combined with assistance of ultrasonic waves, full contact of the enzyme and a substrate is promoted, the enzymolysis efficiency is greatly improved, and compared with a traditional single enzymolysis method or a non-ultrasonic-assistance method, the yield of the anti-fatigue peptide is improved by 20% or above; meanwhile, through a macroporous adsorption resin and ultrafiltration membrane two-step purification combination technology, impurities are effectively removed, the product purity is improved by 30% or above, the molecular weight distribution is more uniform, and the product quality is improved.

This invention discloses a chicken embryo fibroblast, its preparation method, and its application, relating to the field of biopharmaceutical technology. The preparation method of the chicken embryo fibroblast includes the following steps: S1, providing chicken embryos and obtaining the muscle tissue of the chicken embryos; S2, mixing the muscle tissue with a plant-derived compound digestion solution, and sequentially performing digestion, purification, and primary culture treatment using a serum-free special culture medium to obtain primary chicken embryo fibroblasts; wherein, the plant-derived compound digestion solution includes bromelain and papain. This invention, using a plant-derived compound digestion solution and a serum-free special culture medium, significantly improves the batch-to-batch stability, cell yield, and cell purity of the cells, reducing the risk of exogenous viral contamination. Therefore, the prepared chicken embryo fibroblasts exhibit high batch-to-batch stability and good reproducibility in viral culture.

The present application relates to a kind of gentle exfoliating whitening body care composition and its preparation method and application, it is composed of the following weight parts of component: liposome 5-10 parts, papain 0.01-1 part and bromelain 0.005-0.5 part;Wherein, the liposome includes shell and core layer, the shell includes lecithin and phytosterol, the core layer includes camellia nitidissima extract and hydrolyzed royal jelly protein;The liposome includes the following weight parts of raw material: lecithin 2-15 parts, phytosterol 0.1-5 parts, camellia nitidissima extract 0.1-3 parts, hydrolyzed royal jelly protein 0.01-1 part.The composition can realize the multiple effects of skin lightening, removing old and useless cutin, tough skin, while safe, mild and non-irritating.