Extraction method of ganoderma lucidum polysaccharide

An extraction method, technology of Ganoderma lucidum polysaccharides, applied in the field of extraction of Ganoderma lucidum polysaccharides, to achieve the effect of safe solvent, convenient operation and fast speed

Inactive Publication Date: 2015-01-21
牛睦元
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The yield of polysaccharides in the above-mentioned method is not high, so it is necessary to improve the above-mentioned method and design a high-yield extraction method of Ganoderma lucidum polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for extracting ganoderma polysaccharides, the method comprising the steps of:

[0041] Select the dried Ganoderma lucidum, crush it and pass through a 60-mesh sieve to get Ganoderma lucidum powder, add water to the Ganoderma lucidum powder and stir evenly, the weight ratio of Ganoderma lucidum powder to water is 1:7.5, soak in water for 10 hours, add cellulase, fiber The amount of suzyme added is 0.75%, the enzymolysis temperature is 45°C, and the pH value is 5. The above-mentioned raw materials are extracted in a microwave extraction equipment. The power of the microwave is 400-600W, the frequency is 800MHZ, and the enzymolysis is 0.1-0.5h;

[0042] Then add alkaline protease and bromelain, the addition of alkaline protease is 0.45%, the addition of bromelain is 0.4%, the enzymolysis temperature is 50 ° C, the pH value is 7.2, and the above-mentioned raw materials are placed in microwave extraction equipment for extraction. The power of the microwave is 500W, ...

Embodiment 2

[0050]A method for extracting ganoderma polysaccharides, the method comprising the steps of:

[0051] Select the dried Ganoderma lucidum, crush it and pass through a 40-mesh sieve to obtain Ganoderma lucidum powder, add water to the Ganoderma lucidum powder and stir evenly, the weight ratio of Ganoderma lucidum powder to water is 1:5, soak in water for 8 hours, add cellulase, fiber The amount of suzyme added is 0.5%, the enzymolysis temperature is 45°C, and the pH value is 5. The above-mentioned raw materials are extracted in a microwave extraction equipment. The power of the microwave is 400-600W, the frequency is 800MHZ, and the enzymolysis is 0.1-0.5h;

[0052] Then add alkaline protease and bromelain, the addition of alkaline protease is 0.2%, the addition of bromelain is 0.2%, the enzymolysis temperature is 50 ° C, the pH value is 6.8, and the above-mentioned raw materials are placed in microwave extraction equipment to extract, The power of the microwave is 400W, the fre...

Embodiment 3

[0060] A method for extracting ganoderma polysaccharides, the method comprising the steps of:

[0061] Select the dried Ganoderma lucidum, crush it and pass it through a 80-mesh sieve to obtain Ganoderma lucidum powder, add water to the Ganoderma lucidum powder and stir evenly, the weight ratio of Ganoderma lucidum powder to water is 1:10, soak in water for 16 hours, add cellulase, fiber The amount of suzyme added is 0.9%, the enzymolysis temperature is 45°C, and the pH value is 5. The above-mentioned raw materials are extracted in a microwave extraction equipment. The power of the microwave is 400-600W, the frequency is 800MHZ, and the enzymolysis is 0.1-0.5h;

[0062] Then add alkaline protease and bromelain, the addition of alkaline protease is 0.6%, the addition of bromelain is 0.5%, the enzymolysis temperature is 50 ° C, the pH value is 7.5, and the above-mentioned raw materials are placed in microwave extraction equipment for extraction. The power of the microwave is 500...

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PUM

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Abstract

The invention belongs to the technical field of polysaccharide extraction and particularly relates to an extraction method of a ganoderma lucidum polysaccharide. The extraction method of the ganoderma lucidum polysaccharide comprises the following steps: selecting air-dried ganoderma lucidum; grinding the ganoderma lucidum, and screening by a 40-80-mesh sieve to obtain ganoderma lucidum powder; adding water into the ganoderma lucidum powder and uniformly stirring, wherein the weight ratio of the ganoderma lucidum powder to the water is 1: (5-10); soaking the ganoderma lucidum powder in the water for 8 to 16 hours; adding cellulase for enzymolysis; then adding alkaline protease and bromelain; carrying out enzymolysis under the microwave condition; after enzymolysis, concentrating and decolorizing; centrifuging; taking supernate to carry out concentration and alcohol precipitation; centrifuging again; washing precipitates with ethanol; after freeze-drying, grinding to obtain the ganoderma lucidum polysaccharide. When the method disclosed by the invention is adopted to extract the polysaccharide from the ganoderma lucidum, the action condition is mild due to the adoption of enzymes, fat and cellulose in the ganoderma lucidum are subjected to enzymolysis by adopting various enzymes to extract the polysaccharide from the ganoderma lucidum, and thus, the utilization rate of the ganoderma lucidum is increased. The polysaccharide obtained by adopting the method provided by the invention not only has high yield, but also has high purity.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to an extraction method of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum polysaccharides can prevent tumors and inhibit tumor growth, and have been used in clinical treatment of tumors. Experiments have proved that most of the anti-tumor effects of Ganoderma lucidum polysaccharides are as a biological response regulator, by enhancing the host's immune regulation function, that is, host-mediated anti-tumor effect. Ganoderma lucidum polysaccharide has no cytotoxic effect, that is, it cannot directly kill tumor cells, but enhances the phagocytosis of M, promotes the proliferation of T lymphocytes, enhances the cytotoxic effect of T cells, and induces certain immune factors Produce and other pathways to inhibit tumor growth. [0003] Ganoderma lucidum alkali-soluble polysaccharide has a strong ability to inhibit the gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 迟宗磊藏伟功丁振洋
Owner 牛睦元
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