3 results about "Polystyrene latex" patented technology

This invention discloses a rapid human interleukin-4 test strip based on a double-antibody sandwich method. The test strip includes a sample pad, a latex pad, a conjugate pad, a nitrocellulose membrane, and an absorbent pad. The latex pad is made of latex microspheres labeled with CKIgY monoclonal antibody and red polystyrene latex microspheres. The conjugate pad is made of latex microspheres labeled with IL-mouse monoclonal antibody and red polystyrene latex microspheres. A detection line and a control line are located in the center of the nitrocellulose membrane. The detection line contains a protein conjugate for binding with the IL-mouse monoclonal antibody, and the control line contains an IgG monoclonal antibody. An upper shell is located on the outside of the test strip, and a matching lower shell is provided. This invention provides a rapid human interleukin-4 test strip based on a double-antibody sandwich method. This test strip has high sensitivity, low risk of cross-reaction, simple operation, and low cost.

This invention discloses an immunochromatographic assay kit, preparation method, and application for the detection of apolipoprotein E4. The kit includes a test card and sample diluent. The test card comprises a test strip, which includes a PVC base, absorbent paper, a glass fiber pad, and a nitrocellulose membrane. The glass fiber pad is loaded with polystyrene latex microspheres for physical adsorption. The surface of the polystyrene latex microspheres is modified with sulfonyl or hydroxyl groups, and the zeta potential ranges from -15V to -20V. After the polystyrene latex microspheres are uniformly coated on a glass slide and dried, the contact angle between them and water droplets is greater than 90°. The nitrocellulose membrane is loaded with anti-APOE4 antibody, coating solution, and protein A. The sample diluent includes PBS, TWEEN-20, Tetronic 1307, and CHAPS. This invention eliminates the need for monoclonal antibody-labeled latex microspheres, reducing false positives and reagent batch-to-batch variation, and achieving higher sensitivity.

The application discloses a dumbbell-shaped Janus solid base catalyst and a preparation method and application thereof. The dumbbell-shaped Janus solid base catalyst is prepared through the following steps: first, polystyrene latex is prepared through an emulsion polymerization method; then, core-shell structure chlorinated polystyrene particles are obtained by using a seed emulsion polymerization method to polymerize on the polystyrene particles; next, the dumbbell-shaped Janus particles are obtained by using a seed swelling polymerization method with the chlorinated polystyrene particles as seeds; finally, the dumbbell-shaped Janus solid base catalyst is obtained through amination. The method avoids the problem of poor stability of the spherical solid base catalyst, one half of the dumbbell-shaped Janus is hydrophobic pure polystyrene, and the other half is amphiphilic due to the unilateral grafting of the organic base, thereby effectively improving the stability of the particles. Meanwhile, the catalyst is easy to separate, and the reusability of the catalyst is effectively improved. The prepared solid base catalyst can be used in the reaction of ester exchange for preparing biodiesel, and has the characteristics of high activity and simple recovery and separation.