17 results about "IgG.monoclonal" patented technology

The invention relates to the technical field of biological detection, and particularly discloses a bovine brucella antibody immunochromatography detection test strip as well as a preparation method and application thereof. The bovine brucella antibody immunochromatography detection test strip provided by the invention comprises a PVC (Polyvinyl Chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate; the combination pad contains a mouse anti-bovine IgG monoclonal antibody marked by quantum dot microspheres; a detection line T and a quality control line C are arranged on the nitrocellulose membrane; the detection line T is coated with a brucella LPS antigen, and the quality control line C is coated with a goat anti-mouse IgG polyclonal antibody. The bovine brucella antibody immunochromatography detection test strip provided by the invention has the advantages of no subjective influence of a detector, simplicity and convenience in operation, high detection sensitivity and specificity and the like.

The present application relates to the technical field of immunology, and particularly relates to a murine IgG monoclonal antibody, a preparation method and application thereof. The heavy chain variable region of the monoclonal antibody has an amino acid sequence as shown in SEQ ID NO:1; and the light chain variable region has an amino acid sequence as shown in SEQ ID NO:2. The present application further discloses a DNA molecule encoding the antibody, expression of the antibody, and a eukaryotic host cell transformed by the expression vector. The murine IgG monoclonal antibody provided by the present application is preliminarily verified in a 5L bioreactor, and the expression amount is 5.33g / L. It is identified that the monoclonal antibody is used as a blocking agent for evaluation, and the blocking effect is obvious, and plays an important role in the use of blocking agents.

The invention provides an HbA1c antibody compound which comprises an anti-human HbA1c protein monoclonal antibody I, an anti-mouse IgG protein monoclonal antibody II and an anti-mouse IgG monoclonal antibody III which respectively comprise a light chain and a heavy chain, the light chain sequence of the antibody I is shown as SEQ ID NO.1, and the heavy chain sequence of the antibody I is shown as SEQ ID NO.2; the light chain sequence of the antibody II is as shown in SEQ ID NO.3, and the heavy chain sequence of the antibody II is as shown in SEQ ID NO.4; the light chain sequence of the antibody III is as shown in SEQ ID NO.5, and the heavy chain sequence of the antibody III is as shown in SEQ ID NO.6. The invention further provides a latex enhanced immunoturbidimetric kit for quantitatively detecting the content of HbA1c and a preparation method of the latex enhanced immunoturbidimetric kit. The antibody is high in affinity, good in specificity and high in stability, and a kit based on the antibody is excellent in stability, high in sensitivity, accurate in measured value and wide in linearity.

The invention relates to the technical field of biology, in particular to an anti-PLA2R fully human IgG monoclonal antibody and application thereof. The invention provides a human-derived anti-alkali phospholipase A2 receptor antibody PLA2R PL82 screened by using a phage surface display technology, the subtype of the human-derived anti-alkali phospholipase A2 receptor antibody PLA2R PL82 is IgG4, and amino acid sequences of light chain and heavy chain variable regions of the human-derived anti-alkali phospholipase A2 receptor antibody PLA2R PL82 are respectively shown as SEQ ID NO: 6 and SEQ ID NO: 7. The antibody can specifically recognize an alkali phospholipase A2 receptor, the reactivity, the stability and the matrix effect all meet the performance use requirements of a kit, and the antibody plays an important role in alkali phospholipase A2 IgG project application as a quality control product.

The application discloses an anti-MPO human IgG monoclonal antibody and application thereof. Amino acid sequences of CDR1, CDR2 and CDR3 in a heavy chain variable region of the anti-MPO human IgG monoclonal antibody are sequentially shown as SEQ ID No. 1 26-33, 51-58 and 97-111, and amino acid sequences of CDR1, CDR2 and CDR3 in a light chain variable region are sequentially shown as SEQ ID No. 2 26-34, 52-54 and 91-102. Experiments prove that the monoclonal antibody not only has good binding activity with MPO, with a Kd of 0.0088 nM and an EC50 of 1.33 ng / mL, but also has good universality and stability, and can be used as MPO-ANCA quality control instead of a clinical positive sample, and then used for AAV diagnosis, activity evaluation and treatment effect monitoring.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody of mumps virus, a recombinant IgM antibody and application. Wherein the IgG antibody comprises a heavy chain constant region, a light chain constant region, and a heavy chain variable region and a light chain variable region of the IgG monoclonal antibody which is specifically combined with the mumps virus, and the IgG monoclonal antibody is of a mouse source or a rabbit source. According to the present invention, the IgG antibody is modified to obtain the recombinant IgM antibody, and the IgG antibody and the recombinant IgM antibody are not only suitable for the chemiluminescence method, but also show the expected sensitivity amplification factor in other immunological detection methods, such that the low-abundance detection of the specifically bound mumps virus can be easily achieved.

The present application belongs to the field of rapid detection, and provides a small molecule detection kit. The traditional detection of small molecules by immunochromatographic competition inhibition mode is converted into a double-antibody sandwich mode through a conversion column and a general mouse IgG immunochromatographic test strip. The reverse reading signal on the detection line is converted into a positive reading signal, so that the trace existence of small molecules can make the detection line of the test strip appear a signal, and compared with the traditional competitive immunochromatographic method, the present application has the characteristics of high detection sensitivity. The conversion column is filled and formed after the target small molecule or its artificial antigen is covalently coupled with agarose or cellulose gel; the mouse IgG immunochromatographic test strip is composed of five parts, i.e. filter paper, sample pad, combination and release pad (fixed with a probe having signal output capability, i.e. labeled anti-mouse IgG monoclonal antibody 1 colloidal gold or fluorescent microspherical nanomaterial), NC membrane (anti-mouse IgG monoclonal antibody 2 is fixed on the detection line, and Protein G is fixed on the control line), and water absorption paper.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody of HSV-II, a recombinant IgM antibody and application. Wherein the IgG antibody comprises a heavy chain constant region, a light chain constant region, and a heavy chain variable region and a light chain variable region of the IgG monoclonal antibody specifically combined with the herpes simplex virus HSV-II, and the IgG monoclonal antibody is of a mouse source or a rabbit source. According to the present invention, the IgG antibody is modified to obtain the recombinant IgM antibody, and the IgG antibody and the recombinant IgM antibody not only are suitable for the chemiluminescence method, but also show the expected sensitivity amplification factor in other immunological detection methods, such that the low-abundance detection of the specific binding herpes simplex virus HSV-II can be easily achieved.

The application relates to the technical field of biological detection, and particularly discloses a bovine brucella antibody immunochromatographic detection test strip as well as a preparation method and application thereof. The bovine brucella antibody immunochromatographic detection test strip provided by the application comprises a PVC bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate, the combination pad contains quantum dot microsphere labeled mouse anti-bovine IgG monoclonal antibody, the nitrocellulose membrane is provided with a detection T line and a quality control C line, the detection T line is coated with a brucella LPS antigen, and the quality control C line is coated with a goat anti-mouse IgG polyclonal antibody. The bovine brucella antibody immunochromatographic detection test strip provided by the application has the advantages of being not influenced by subjective factors of a tester, simple and convenient operation, high detection sensitivity and specificity and the like.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody of HSV-I, a recombinant IgM antibody and application. Wherein the IgG antibody comprises a heavy chain constant region, a light chain constant region, and a heavy chain variable region and a light chain variable region of an IgG monoclonal antibody specifically combined with herpes simplex virus HSV-I, the IgG monoclonal antibody is of a mouse source or a rabbit source, and the IgG antibody is modified to obtain the recombinant IgM antibody. The IgG antibody and the recombinant IgM antibody are not only suitable for a chemiluminescence method, but also show expected sensitivity magnification times in other immunological detection methods, and are helpful for realizing low-abundance detection of the specific binding herpes simplex virus HSV-I.

The invention provides a fluorescence immunochromatography test strip for detecting free IgE (Immunoglobulin E) and a preparation method of the fluorescence immunochromatography test strip. The preparation method comprises the following steps: treating a second cellulose membrane by adopting a second buffer solution containing a mouse anti-human IgG monoclonal antibody to obtain a filter membrane; treating the third cellulose membrane with a third buffer solution containing a fluorescent microsphere labeled FcERI antigen and a fluorescent microsphere labeled DNP-BSA to obtain a conjugate pad; a detection line and a quality control line are arranged on the fourth cellulose membrane, a detection membrane is obtained, the detection line is coated with a mouse anti-human IgE antibody, and the quality control line is coated with a DNP monoclonal antibody; a sample pad, the filter membrane, the combination pad, the detection membrane and a water absorption material are sequentially loaded on a bottom plate, and the fluorescence immunochromatography test strip for detecting the free IgE is obtained. The fluorescence immunochromatography test strip has the advantages of high sensitivity, high accuracy, good stability, good repeatability, simplicity, easiness in operation and the like, and has a wide application prospect.

The present invention discloses an anti-procalcitonin Fab antibody and a preparation method and application thereof, wherein the heavy chain variable region of the heavy chain has three complementary determining regions CDR1, CDR2 and CDR3, and the amino acid sequences thereof are shown in SEQ ID NO.1, 2 and 3 respectively; the light chain variable region of the light chain has three complementary determining regions CDR1', CDR2' and CDR3', and the amino acid sequences thereof are shown in SEQ ID NO.4, 5 and 6. The anti-procalcitonin Fab antibody provided by the scheme of the present invention is a fully human genetic engineering antibody, which can be effectively used to detect procalcitonin, and can also be used as a labeling antibody to be paired with other mouse PCT IgG monoclonal antibodies for clinical detection, and has the characteristics of high specificity, high sensitivity, low cost, strong anti-interference ability, etc.

The present invention relates to the technical field of livestock immunology, and in particular to a mouse anti-bovine IgG monoclonal antibody and its application. The mouse anti-bovine IgG monoclonal antibody provided by the present invention can specifically bind to bovine IgG, has no cross reaction with other similar proteins, has high affinity with bovine IgG, and has high stability; based on the antibody, the double antibody sandwich ELISA method is used to detect bovine IgG with high sensitivity, specificity and accuracy, and can realize accurate and high-throughput detection of IgG content in bovine serum or other serum analogs or products containing bovine IgG, and has good application prospects in bovine IgG detection.

The invention provides an anti-atherosclerosis IgG monoclonal antibody, belongs to the technical field of biology, and totally comprises four IgG monoclonal antibodies. The IgG monoclonal antibody can be used for directly inhibiting macrophage phagocytosis of o-LDL (oxidized low-density lipoprotein) and formation of foam cells, so that formation of atherosclerotic plaques is blocked; through phagocytosis mediated by the IgG monoclonal antibody, immune cells can be promoted to phagocytize foam cells, and then fading of atherosclerotic plaques is promoted. Based on the two mechanisms, the IgG monoclonal antibody has the prospect of being developed into a novel medicine for resisting atherosclerosis.

This invention discloses a rapid human interleukin-4 test strip based on a double-antibody sandwich method. The test strip includes a sample pad, a latex pad, a conjugate pad, a nitrocellulose membrane, and an absorbent pad. The latex pad is made of latex microspheres labeled with CKIgY monoclonal antibody and red polystyrene latex microspheres. The conjugate pad is made of latex microspheres labeled with IL-mouse monoclonal antibody and red polystyrene latex microspheres. A detection line and a control line are located in the center of the nitrocellulose membrane. The detection line contains a protein conjugate for binding with the IL-mouse monoclonal antibody, and the control line contains an IgG monoclonal antibody. An upper shell is located on the outside of the test strip, and a matching lower shell is provided. This invention provides a rapid human interleukin-4 test strip based on a double-antibody sandwich method. This test strip has high sensitivity, low risk of cross-reaction, simple operation, and low cost.

The present invention relates to the technical field of livestock immunology, and in particular to a mouse anti-bovine IgG monoclonal antibody and its application. The mouse anti-bovine IgG monoclonal antibody provided by the present invention can specifically bind to bovine IgG, has no cross reaction with other similar proteins, has high affinity with bovine IgG, and has high stability; based on the antibody, the double antibody sandwich ELISA method is used to detect bovine IgG with high sensitivity, specificity and accuracy, and can realize accurate and high-throughput detection of IgG content in bovine serum or other serum analogs or products containing bovine IgG, and has good application prospects in bovine IgG detection.

This invention relates to a fluorescent immunochromatographic test strip for detecting free IgE, comprising a base plate, a sample pad disposed on the base plate, a conjugate pad, a detection membrane, and an absorbent material sequentially overlapped on the base plate, as well as a detection line and a control line disposed on the detection membrane; the fluorescent immunochromatographic test strip for detecting free IgE further includes a filter membrane disposed on the base plate, with one end overlapping the sample pad and the other end overlapping the conjugate pad; the filter membrane is coated with mouse anti-human IgG monoclonal antibody, the conjugate pad is coated with fluorescent microsphere-labeled FcERI antigen and fluorescent microsphere-labeled DNP-BSA, the detection line is coated with mouse anti-human IgE antibody, and the control line is coated with DNP monoclonal antibody. This fluorescent immunochromatographic test strip has advantages such as high sensitivity, high accuracy, good stability, good repeatability, and ease of operation, and has broad application prospects.