7 results about "IgG.monoclonal" patented technology

The present application relates to the technical field of immunology, and particularly relates to a murine IgG monoclonal antibody, a preparation method and application thereof. The heavy chain variable region of the monoclonal antibody has an amino acid sequence as shown in SEQ ID NO:1; and the light chain variable region has an amino acid sequence as shown in SEQ ID NO:2. The present application further discloses a DNA molecule encoding the antibody, expression of the antibody, and a eukaryotic host cell transformed by the expression vector. The murine IgG monoclonal antibody provided by the present application is preliminarily verified in a 5L bioreactor, and the expression amount is 5.33g / L. It is identified that the monoclonal antibody is used as a blocking agent for evaluation, and the blocking effect is obvious, and plays an important role in the use of blocking agents.

The invention provides an HbA1c antibody compound which comprises an anti-human HbA1c protein monoclonal antibody I, an anti-mouse IgG protein monoclonal antibody II and an anti-mouse IgG monoclonal antibody III which respectively comprise a light chain and a heavy chain, the light chain sequence of the antibody I is shown as SEQ ID NO.1, and the heavy chain sequence of the antibody I is shown as SEQ ID NO.2; the light chain sequence of the antibody II is as shown in SEQ ID NO.3, and the heavy chain sequence of the antibody II is as shown in SEQ ID NO.4; the light chain sequence of the antibody III is as shown in SEQ ID NO.5, and the heavy chain sequence of the antibody III is as shown in SEQ ID NO.6. The invention further provides a latex enhanced immunoturbidimetric kit for quantitatively detecting the content of HbA1c and a preparation method of the latex enhanced immunoturbidimetric kit. The antibody is high in affinity, good in specificity and high in stability, and a kit based on the antibody is excellent in stability, high in sensitivity, accurate in measured value and wide in linearity.

The application discloses an anti-MPO human IgG monoclonal antibody and application thereof. Amino acid sequences of CDR1, CDR2 and CDR3 in a heavy chain variable region of the anti-MPO human IgG monoclonal antibody are sequentially shown as SEQ ID No. 1 26-33, 51-58 and 97-111, and amino acid sequences of CDR1, CDR2 and CDR3 in a light chain variable region are sequentially shown as SEQ ID No. 2 26-34, 52-54 and 91-102. Experiments prove that the monoclonal antibody not only has good binding activity with MPO, with a Kd of 0.0088 nM and an EC50 of 1.33 ng / mL, but also has good universality and stability, and can be used as MPO-ANCA quality control instead of a clinical positive sample, and then used for AAV diagnosis, activity evaluation and treatment effect monitoring.

The present application belongs to the field of rapid detection, and provides a small molecule detection kit. The traditional detection of small molecules by immunochromatographic competition inhibition mode is converted into a double-antibody sandwich mode through a conversion column and a general mouse IgG immunochromatographic test strip. The reverse reading signal on the detection line is converted into a positive reading signal, so that the trace existence of small molecules can make the detection line of the test strip appear a signal, and compared with the traditional competitive immunochromatographic method, the present application has the characteristics of high detection sensitivity. The conversion column is filled and formed after the target small molecule or its artificial antigen is covalently coupled with agarose or cellulose gel; the mouse IgG immunochromatographic test strip is composed of five parts, i.e. filter paper, sample pad, combination and release pad (fixed with a probe having signal output capability, i.e. labeled anti-mouse IgG monoclonal antibody 1 colloidal gold or fluorescent microspherical nanomaterial), NC membrane (anti-mouse IgG monoclonal antibody 2 is fixed on the detection line, and Protein G is fixed on the control line), and water absorption paper.

The application relates to the technical field of biological detection, and particularly discloses a bovine brucella antibody immunochromatographic detection test strip as well as a preparation method and application thereof. The bovine brucella antibody immunochromatographic detection test strip provided by the application comprises a PVC bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are arranged on the PVC bottom plate, the combination pad contains quantum dot microsphere labeled mouse anti-bovine IgG monoclonal antibody, the nitrocellulose membrane is provided with a detection T line and a quality control C line, the detection T line is coated with a brucella LPS antigen, and the quality control C line is coated with a goat anti-mouse IgG polyclonal antibody. The bovine brucella antibody immunochromatographic detection test strip provided by the application has the advantages of being not influenced by subjective factors of a tester, simple and convenient operation, high detection sensitivity and specificity and the like.

This invention discloses a rapid human interleukin-4 test strip based on a double-antibody sandwich method. The test strip includes a sample pad, a latex pad, a conjugate pad, a nitrocellulose membrane, and an absorbent pad. The latex pad is made of latex microspheres labeled with CKIgY monoclonal antibody and red polystyrene latex microspheres. The conjugate pad is made of latex microspheres labeled with IL-mouse monoclonal antibody and red polystyrene latex microspheres. A detection line and a control line are located in the center of the nitrocellulose membrane. The detection line contains a protein conjugate for binding with the IL-mouse monoclonal antibody, and the control line contains an IgG monoclonal antibody. An upper shell is located on the outside of the test strip, and a matching lower shell is provided. This invention provides a rapid human interleukin-4 test strip based on a double-antibody sandwich method. This test strip has high sensitivity, low risk of cross-reaction, simple operation, and low cost.

This invention relates to a fluorescent immunochromatographic test strip for detecting free IgE, comprising a base plate, a sample pad disposed on the base plate, a conjugate pad, a detection membrane, and an absorbent material sequentially overlapped on the base plate, as well as a detection line and a control line disposed on the detection membrane; the fluorescent immunochromatographic test strip for detecting free IgE further includes a filter membrane disposed on the base plate, with one end overlapping the sample pad and the other end overlapping the conjugate pad; the filter membrane is coated with mouse anti-human IgG monoclonal antibody, the conjugate pad is coated with fluorescent microsphere-labeled FcERI antigen and fluorescent microsphere-labeled DNP-BSA, the detection line is coated with mouse anti-human IgE antibody, and the control line is coated with DNP monoclonal antibody. This fluorescent immunochromatographic test strip has advantages such as high sensitivity, high accuracy, good stability, good repeatability, and ease of operation, and has broad application prospects.