Novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses

A coronavirus and sars-cov-2 technology, applied in the field of human medicine and health, can solve the problems of large matrix effect background interference, low detection sensitivity, and incapable of quantitative detection, and achieve high sensitivity, simple operation, and easy promotion and use.

Pending Publication Date: 2020-07-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ELISA requires expensive equipment, professionals proficient in complex operations, and takes a long time; while the immunocolloidal gold method has the advantages of rapid detection, low cost, and convenient operation, but there are still some defects, such as quantitative detection and low detection sensitivity. , the matrix effect is obvious, the background interference is large, etc.

Method used

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  • Novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Such as figure 1 As shown, a quantum dot microsphere immunochromatographic test strip for detecting antibodies against novel coronavirus SARS-CoV-2, the test strip includes a bottom plate 8, a sample pad 1, a binding pad 2, a nitrocellulose membrane 6 and a water-absorbent The pad 7, the sample pad 1, the binding pad 2, the nitrocellulose membrane 6 and the water-absorbing pad 7 are pasted end to end on the bottom plate 8 in sequence, overlapping by 2 mm. The binding pad 2 is coated with SARS-CoV-2N protein-quantum dot microspheres and goat anti-rabbit IgG-quantum dot microsphere markers, and the nitrocellulose membrane 6 is sequentially coated with mouse anti- The detection line T1 3 of human IgG monoclonal antibody, the detection line T2 4 of coated mouse anti-human IgM monoclonal antibody and the quality control line C 5 of coated rabbit IgG.

[0034] The material of the bottom plate 8 is PVC or hard non-absorbent material; the material of the sample pad 1 is glass ...

Embodiment 2

[0057] Example 2 Utilize the test strip prepared in Example 1 to detect novel coronavirus SARS-CoV-2 antibody

[0058] (1) Dilute the sample to be tested by different multiples, add it to colloidal gold test strip chromatography for 10-15 minutes, and judge the result visually to determine the detection limit (maximum dilution factor, 15 times);

[0059] (2) Add the sample to be tested with the same dilution factor to the sample pad of the above-mentioned test strip and perform chromatography for 10-15 minutes, then use a fluorescence detector to measure the corresponding fluorescence intensity value of the sample for result judgment, and determine the detection limit (maximum dilution multiple, 200 times);

[0060] (3) The results show that the above-mentioned test strips are an order of magnitude more sensitive than the colloidal gold test strips;

[0061] (4) And a total of 60 other non-new coronavirus pneumonia patient serum samples were tested, and the results showed tha...

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Abstract

The invention discloses a novel fluorescence immunochromatography test strip for joint inspection of SARS-CoV-2 IgG-IgM antibodies of coronaviruses. The test strip comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are sequentially connected end to end and adhered to the bottom plate; the combination pad is coated with an SARS-CoV-2 structural protein-marker and a goat anti-rabbit IgG-marker, and the nitrocellulose membrane is provided with a detection line T1 coated with a mouse anti-human IgG monoclonal antibody, a detection line T2 coated with a mouse anti-human IgM monoclonal antibody and a quality control line C coated withrabbit IgG. When the test strip is used for quantitatively detecting SARS-CoV-2 IgG and IgM antibodies, the detection sensitivity is high, and the specificity is good and can reach 96%; the batch-to-batch difference is small, and good repeatability is achieved; the test strip can be stored for half a year at normal temperature without reducing the sensitivity and has good stability; the test strip is simple to operate and low in cost, can quickly and quantitatively detect the levels of SARS-CoV-2 IgG and IgM antibodies in a human body, assists a nucleic acid detection means, and provides powerful support for epidemic situations.

Description

technical field [0001] The invention belongs to the field of human medicine and hygiene, and in particular relates to a fluorescent immunochromatographic test strip for joint detection of novel coronavirus SARS-CoV-2 IgG-IgM antibodies. Background technique [0002] Novel coronavirus pneumonia (Novel coronavirus pneumonia) is an acute respiratory infectious disease caused by 2019 novel coronavirus (SARS-CoV-2) infection. Since the outbreak in Wuhan at the end of 2019, as of February 25, the cumulative number of confirmed cases has exceeded 77,000. Thanks to the joint efforts of medical and other industries and friendly assistance from foreign countries, the epidemic has been gradually brought under control. The detection and diagnosis of the new coronavirus is an important link in the effective control of the epidemic, and efficient virus detection technology has also become a hot spot in research and development. [0003] The Fifth Trial Edition of the Diagnosis and Treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/58G01N33/577G01N33/569G01N33/558
CPCG01N33/6854G01N33/588G01N33/582G01N33/587G01N33/577G01N33/56983G01N33/558G01N2333/165G01N2469/20
Inventor 刘斐王彬单衍可刘婧李嘉豪
Owner NANJING AGRICULTURAL UNIVERSITY
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