Retinol conjugated protein detection kit
A detection kit and protein-binding technology, which is applied in the field of medical immunity, can solve the problems of reagent quality degradation, easy coalescence, poor reagent stability, etc., and achieve the effect of simple detection, high sensitivity and low production cost
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Embodiment 2
[0037] The determination of embodiment 2 retinol binding protein
[0038] Detection tool: Hitachi 7060 automatic analyzer.
[0039] Analysis method: two-point endpoint method; main wavelength: 570nm, secondary wavelength: -: sample volume: 2ul (microliter); R1: 200ul; R2: 50ul; reaction direction: rising; measurement temperature: 37°C; sample mixed with R1 After mixing, read the absorbance A1 at 10 seconds; add R2 at 3-5 minutes, and read the absorbance A2 after 5 minutes. The reaction absorbance was calculated as the difference between A2 and A1.
[0040] Calculation method: multi-point calibration, the dose / response curve is made according to the absorbance and reference serum value, and the sample content can be calculated on the dose / response curve according to its absorbance value.
Embodiment 3
[0041] Example 3 Retinol-binding protein detection kit performance evaluation
[0042] 1. Analytical Sensitivity Assessment
[0043] Use 5% bovine serum albumin solution as a blank sample, and the blank sample should not contain the analyte. 20 consecutive detections were performed on the biochemical analyzer, and the mean and standard deviation SD of the 20 results were calculated. The detection limit of the reporting method is the mean value of the blank plus two standard deviations (+2SD). As can be seen from Table 1, the sensitivity is 2.68mg / L.
[0044] Table 1
[0045] 2. High value linear evaluation
[0046] 1 part of low-value serum (10mg / L) and 1 part of high-value serum (100mg / L) according to the ratio of 9:1, 4:1, 2:1, 1:2, 1:4, 1:9 (sample sequence number 1 to 6), prepared 6 samples with different concentrations, each sample was measured twice, as can be seen from Table 2,
[0047] The highest detection range of the detection kit of the present invention ca...
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