50results about How to "Detection economy" patented technology

The invention relates to a nondestructive testing method of a concrete structure, which comprises the steps: based on the original infrared imaging system, an ultrasonic excitation source is additionally arranged on a tested object; after the ultrasonic excitation source is used, infrared pulse excitation continues to be adopted for combining ultrasound damage location with infrared depth analysis, which protects the tested object, avoids detection of the whole thermography area, aims at detection of the determined image processing area, and is beneficial to improving the detection efficiency; and then, a standard part is adopted for sample detection, and the depth information of the tested object is reversely deduced. The nondestructive testing method is feasible, and has low requirement on precision of detection apparatuses.

The invention relates to a method for long-term preservation and rapid recovery of luminous bacterium strain, and belongs to the technical field of environmental detection and evaluation. Through exploitation of a special bacterium freeze-stored medium and a recovery solution, the method provided by the invention realizes long-term preservation and rapid recovery of luminous bacterium strain, saves a testing cost, greatly shortens toxicity testing time, and provides possibility for chemical pollutant toxicity rapid testing. The method is simple and economic, can produce effects fast, prolongs strain preservation time, allows a low experimental operation technology level, and is convenient for popularization and utilization.

The invention discloses a beta 2-microglobulin detection kit which comprises a reagent R1 and a reagent R2, and the reagent R1 is a buffer solution; the reagent R2 is a mixture of beta 2-microglobulin antibody sensitization polystyrene latex particles and a buffer solution. The detection kit has the advantages that the detection is simple and rapid, the sensitivity is high, the accuracy is good, the disturbance resistance is high and the production cost is low. An adopted beta 2-microglobulin detection method is latex enhanced immunoturbidimetry, the beta 2-microglobulin detection is more economical, convenient and rapid with the method, and the beta 2-microglobulin detection kit is suitable for automatic biochemistry analyzers in vast majority of hospitals, and particularly suitable for realizing rapid quantitative detection on emergency treatment.

The invention discloses a kit for testing lipoprotein a(Lp(a)). The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, and the reagent R2 is a mixture of lipoprotein a(Lp(a)) antibody sensitized polystyrene latex particles and the buffer solution. The kit has the advantages of simplicity and rapidness in detection, high sensitivity, good accuracy, high anti-interference capacity and low production cost; a method for detecting the lipoprotein a(Lp(a)) is latex enhanced immunoturbidimetry, with the adoption of the method, lipoprotein a(Lp(a)) can be detected more economically, more conveniently and more quickly, and the kit is suitable for automatic biochemical analyzers in most hospitals, and in particular, quick and quantitative detection can be realized during emergency treatment.

The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.

The invention discloses a preparation method for phosphorescent quantum dots Mn-ZnS and a method for using phosphorescent quantum dots Mn-ZnS for detecting iron ions with different forms in a solution, aiming at selectively detecting the iron ions with different forms in the solution by virtue of phosphorescent changes at a room temperature. According to the method, a deoxidant and an inducer do not need to be added during detection for the iron ions with different forms in the solution, and the interferences of background fluorescence and scattered light can be avoided; meanwhile, a complex sample pre-treatment process is not needed during detection for the iron ions with different forms in the solution; and although quantum-dot fluorescence analysis method is already widely applied, attentions on the phosphorescent properties of quantum dots and the application thereof in analysis and detection are few. Therefore, the phosphorescent quantum dots Mn-ZnS prepared by the preparation method disclosed by the invention are good in water solubility and stability, and great in application prospect in the aspects of detection for a solution or water sample and the like.

The invention discloses a non-diagnostic purpose rapid detection method for Escherichia coli O157:H7. The rapid detection method comprises the following steps: combining an antibody with an enzyme byusing a Au-Pt-SiO2 composite nanosphere as a carrier to form a signal transduction probe; enriching and separating target substances in a sample by using the superparamagnetism of an immunomagnetic probe; and converting the signal of the detected target bacterium Escherichia coli O157:H7 into a glucose molecule signal, and reading the glucose molecule signal by a portable glucometer. The economic,simple, rapid and sensitive novel method for detecting Escherichia coli O157:H7 in foods is constructed on the basis of the glucometer and two nano-compounds capture probe and signal transduction probe.

The invention relates to a urine microalbumin detection kit and a preparation method thereof. The detection kit is prepared from a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from buffer solution (1), inorganic salt ions (1), accelerator (1), stabilizer (1) and preservative (1), and the reagent R2 is prepared from buffer solution (2), inorganic salt ions (2), preservative (2), stabilizer (2), suspending aid (1) and anti-human albumin antibody. The preparation method of the detection kit disclosed by the invention comprises the steps of preparing reagents according to constituent content; mixing a sample to be tested with the reagent R1 and the reagent R2 to enable the sample, the reagent R1 and the reagent R2 fully react; utilizing a full-automatic biochemical analyzer to measure an absorbance difference value after reaction; calculating out the concentration of urine microalbumin in the sample according to an absorbance change value. The detection kit disclosed by the invention has the advantages of low equipment cost, high sensibility, good accuracy and precision, easiness to store, good data repeatability and wide application to clinical biochemical analyzers.

The invention relates to a test tube test method specially aimed at the genetic toxicity effect of chemical pollutants, which belongs to the technical category of environmental detection and evaluation. Design and invent a special test tube for SOS / umu genotoxicity detection, use the test tube as a container for bacterial culture, toxicity exposure, damage repair, color development, and test, use a pipette (or pipette) to transfer the solution, bacteria The solution was mixed and oscillated by a constant temperature oscillator in a water bath, and the absorbance was measured by a visible spectrophotometer. The simple, economical, green and accurate detection of chemical pollutants and genotoxic effects of environmental samples is realized, and it is also convenient for the promotion and popularization of the method.

The invention discloses an alpha 1-microglobulin detection kit. The alpha 1-microglobulin detection kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution; the reagent R2 is a mixture of an alpha 1-microglobulin antibody-sensitization polystyrene latex particles and the buffer solution. The alpha 1-microglobulin detection kit disclosed by the invention has the advantages that 1, the detection is simple and quick, the sensitivity is high, the accuracy is good, the anti-jamming capacity is strong, and the production cost is low; 2, an alpha 1-microglobulin detection method adopted by the invention is a latex-enhanced turbidimetric immunoassay method, so that the alpha 1-microglobulin detection method enables the detection of alpha 1-microglobulin to be more economic, more convenient and quicker, is suitable for an automatic biochemical analyzer in most hospitals, and quick quantitative detection on emergency treatment can be specially realized.

The invention provides a hybridoma cell strain capable of secreting a T-2 toxin monoclonal antibody and also provides the T-2 toxin monoclonal antibody secreted by the cell. The monoclonal antibody has the ascite potency ratio of 1:50000 which is superior to that of the existing monoclonal antibody. Meanwhile, the cross reaction rate of the monoclonal antibody and HT-2 toxin reaches up to 92.8%, and the cross reaction rate of the monoclonal antibody and various other antigens is lower than 0.1%. An immunoaffinity column-liquid chromatography containing the monoclonal antibody can be used for simultaneously and efficiently separating and extracting T-2 toxin and HT-2 toxin in samples and is accurate, economic and effective.

The invention belongs to the technical field of tetracycline detection, particularly relates to application of a sulfur quantum dot as a fluorescent probe in tetracycline detection, and provides new application of the sulfur quantum dot in tetracycline detection in order to establish an economic, rapid, sensitive and high-selectivity tetracycline detection method, namely application of the sulfur quantum dot as the fluorescent probe in tetracycline detection, the characteristic that the detection of the tetracycline by the sulfur quantum dot has relatively high selectivity is utilized, the tetracycline concentration by the sulfur quantum dot is taken as a fluorescent probe, the tetracycline concentration in a to-be-detected sample can be measured according to the linear relation between the fluorescence intensity ratio of the sulfur quantum dot fluorescence before and after the tetracycline to-be-detected sample is added, and the detection process is rapid and sensitive. Therefore, the defects of long detection time, low sensitivity and the like in a traditional tetracycline detection technology are overcome, economic, rapid and sensitive detection of tetracycline antibiotics is realized, and the sulfur quantum dot has huge application potential in rapid and sensitive detection of food matrix tetracycline.

The invention relates to a method for detecting material and capacity of a distribution transformer winding, which orderly comprises the following steps: step 1, counting the distribution of a material parameter and a capacity parameter of the winding; step 2, dividing a distribution interval; step 3, determining core data; step 4, dividing the risk grade, and step 5, judging whether the distribution transformer has a problem. The method for detecting material and capacity of the distribution transformer winding in the invention has the following advantages: through the detection method, the distribution transformer of the suspected winding material or the capacity faking problem in large-scale distribution transformer can be primarily selected quickly; then, the inspection personnel confirms the distribution transformers having the suspected problem one by one, thereby ensuring stable work of the distribution transformer and ensuring the safety performance of the whole power distribution system; the whole detection process is economic, reliable in result and short for the period; the detection means is intelligent and efficient; different core data are set to adapt to different detection targets, thereby meeting different detection requirements.

The invention discloses a method for detecting the Fenghua21 watermelon seed authenticity and purity. The method comprises the following steps that 1, polymorphic stable SSR primers in Fenghua21 maleparents, female parents and hybrids are screened out; 2, an SSR fingerprint spectrum of Fenghua21 is prepared; 3, SSR amplification band patterns of to-be-detected watermelon seeds are compared with the Fenghua21 SSR fingerprint spectrum prepared in step 2, and if comparison results are consistent, the to-be-detected watermelon seeds are judged as true Fenghua21 watermelon hybrid seeds. The methodfor detecting the Fenghua21 watermelon seed authenticity and purity has the advantages that the method is not affected by an external environment, detection can be completed indoors, and watermelon hybrids which are closely related can be identified, so that the method for detecting the Fenghua21 watermelon seed authenticity and purity is accurate, reliable, fast, convenient and economic.

The invention belongs to the field of immunological detection and particularly relates to a method for evaluating the blocking activity of an immune checkpoint molecular blocking antibody by magneticbeads. Magnetic beads with solid-phase anti-tag molecular antibodies and immune checkpoint molecular protein expressing label molecules (label I) subjected to solid-phase on the surfaces of the magnetic beads are used; the magnetic beads can be specifically bound with a ligand of an immune checkpoint molecule with another label molecule (label II), and then a label II specific binding antibody labeled by a biologic substance (fluorescent or visible light) is used for quantitatively detecting the immune checkpoint molecular protein / ligand molecular protein compound on the surface of the magnetic bead. When the blocking antibody of the immune checkpoint molecule, the magnetic beads with the immune checkpoint molecule protein in the solid phase and the ligand protein coexist, the blocking activity of the antibody is inversely proportional to the final luminous intensity of the magnetic beads, so that quantitative analysis on the blocking activity of the antibody is realized through lightintensity detection. The magnetic bead method is simple to operate, quick in reaction, economical and time-saving.

The invention relates to a kit for detecting the methylation degree of DNA (Deoxyribonucleic Acid) based on a gold nanoparticle probe as well as a detection method and application of the kit. The kit comprises the gold nanoparticle probe, wherein the gold nanoparticle probe comprises gold nanoparticles and an upstream primer for performing methylation specific polymerease chain reaction; sulfydryl is modified at the 5'-terminal of the upstream primer; the upstream primer is coupled with the gold nanoparticles by the sulfydryl; the particle sizes of the gold nanoparticles are 10 to 15nm. According to the kit as well as the detection method and application thereof disclosed by the invention, by designing a standard color chart, the methylation degree of to-be-detected DNA can be detected quickly, simply, conveniently and economically in real time according to the color of the system; in addition, a standard curve of ultraviolet absorbancy degree and DNA methylation percentage is designed; related data are substituted into the standard curve by an ultraviolet visible light absorbance spectrum of a test system, so that an accurate DNA methylation percentage can be obtained; no valuable instrument is needed, and a detection method is simpler, more convenient and more economical; higher practical value is obtained.

The invention discloses a full-range C-reactive protein detection kit, which comprises a reagent R1 and a reagent R2. The reagent R1 is a proper buffer solution; the reagent R2 is formed by the following steps: sensitizing two or more styrene latex with different average grain diameters by using an anti-human C-reactive protein antibody; placing the sensitized styrene latex with the different average grain diameters into the proper buffer solution respectively to form reagents; and finally mixing the reagents in different proportions, wherein the reagent R2 contains 0.8 to 3.5 mg / mL of the styrene latex combined with the anti-human C-reactive protein antibody. The full-range C-reactive protein detection kit not only can measure lower CRP content but also can measure high CRP content, and has the advantages of high sensitivity and stability and accurate measurement.

The invention relates to a dioxin detection device and device method thereof. The method is based on biological affinity and comprises the following steps: contacting the detection device with the sample containing dioxin or dioxin-like material; generating a screening effect on the electrode surface using a biological identification element on the detection device and the affinity between the dioxin or dioxin-like material; measuring the current signal difference between the post-testing current signal and the before-testing current signal and comparing the standard solution containing the dioxin or dioxin-like material with the corresponding current signal difference, thereby determining the concentration of the dioxin or dioxin-like material in sample.

The invention provides a method for rapidly determining the content of mercaptoacetic acid. The method comprises the following steps: oxidizing sulfydryl by iodine under an alkaline condition; limiting the reaction speed through IO3<->generated by reaction; and determining the titration end point to obtain the content of mercaptoacetic acid. According to the method for rapidly determining the content of mercaptoacetic acid, the recovery rate of a mercaptoacetic acid titration method reaches 98%; meanwhile, the method is stable.

The invention provides an iodide ion recognition probe and a preparation method thereof. The preparation method comprises the following steps: reacting 9,9-bis(6-bromohexyl)-fluorene-2,7-dicarbaldehyde serving as a raw material with 2,5-diaminopyridine under the condition of anhydrous LiCl serving as a catalyst to obtain an intermediate product; and under the condition that N,N-dimethylacetamide is taken as a solvent, reacting the intermediate product with trimethylamine to obtain a pyridine fluorenyl fluorescent conjugated polymer. The synthesis method is simple, industrial production is easy, iodine ions can be rapidly, sensitively, accurately and economically detected, the efficiency is high in an actual application process, and the probe and method are particularly suitable for detection of micro and trace iodine ions. The fluorescence probe disclosed by the invention is high in iodine ion detection selectivity, the lowest detection limit is 4.68*10<-7> mol / L, a good linear quantitative relation is shown in a range of 0-25 [mu]mol / L, and the fluorescence probe is suitable for trace detection.

The invention discloses a workplace low-energy-consumption electrochemical sensor. The sensor comprises a shell, and a control device, a detecting device, a calibrating device and a sampling device which are arranged in the shell. The detecting device comprises a precheck chamber and a detecting chamber. An electric coupling detector and a spectrum detector are arranged in the detecting chamber. The calibrating device comprises a calibrating controller, a standard substance manager, a blank air sampler and a data processor. By means of the simple sensor, a plurality of same or different samples can be detected, the defects that when power is provided for an existing detector through a detecting pump, the structure is complicated and control is not flexible are avoided, mechanical failuresare greatly reduced, the loads on the low-energy-consumption electrochemical sensor are relieved, and sample detection is more precise, more economical and simpler.

The invention provides a system and a method for detecting a heat treatment state of a high-steel-grade thick-wall pipe fitting based on deep learning. The method comprises the following steps: 1, acquiring metallographic structure images of the inner surface and the outer surface of a to-be-detected area of the high-steel-grade thick-wall pipe fitting; 2, establishing a data set, and dividing the data set into a training set and a test set; 3, constructing a convolutional neural network, and training the constructed convolutional neural network to obtain a trained convolutional neural network; predicting the trained convolutional neural network, and classifying prediction results to obtain a classification result graph; 4, predicting the to-be-detected high-steel-grade thick-wall pipe fitting subjected to heat treatment, judging a prediction result, and further judging whether the to-be-detected high-steel-grade thick-wall pipe fitting subjected to heat treatment is qualified or not; the aim of economically, efficiently, objectively and accurately inspecting the heat treatment state of the high-steel-grade rear wall pipe fitting is achieved.

The invention relates to a pillararene modification cyanide ion fluorescence detection test paper and a preparation method thereof and a detection method thereof. The cyanide ion fluorescence detection test paper is oxidized by the filter paper and placed in the pillararene solution for immersion treatment, then immersed in the sodium borohydride solution after washing, washed, dried, and then immersed in the 10-methyl acridine solution, after cleaning and drying, cyanide ions used for detecting the 10-3~10-7M concentration range are obtained. The method is advantaged in that the cyanide ion fluorescence detection test paper is used for detecting the cyanide ion concentration in the solution, the to-be-detected solution is dropped to the test paper for detection, through observing change of fluorescence intensity on the test paper, the cyanide ion concentration can be quantitatively detected through naked eyes, the response speed is fast, the result can be rapidly determined, and the cyanide ion concentration in the solution can be simply, conveniently, rapidly and economically detected.

The invention provides a nucleic acid aptamer-based lung metastatic osteosarcoma tissue immunofluorescence kit. The nucleic acid aptamer-based lung metastatic osteosarcoma tissue immunofluorescence kit comprises a), an antigen repairing liquid; b), a washing liquid; c), a nucleic acid aptamer dilution liquid; d), a blocking liquid. In applications, Cy5 labelled nucleic acid aptamer is taken as a fluorescence probe for labeling of paraffin embedded lung metastatic osteosarcoma tissue. The nucleic acid aptamer is capable of realizing specific combination with lung metastatic osteosarcoma cells,and combination with other cells is impossible to realize, so that the nucleic acid aptamer is taken as a novel molecule probe with high specific recognition capacity on lung metastatic osteosarcoma,and possesses important research meaning in early stage diagnosis and treatment of osteosarcoma susceptible to pulmonary metastasis. In applications, paraffin section immunofluorescence dyeing is adopted, pathological diagnosis on lung metastatic osteosarcoma tissue can be realized without low temperature storage, the specificity is high, the sensitivity is high, operation is simple, equipment requirement is low, positioning performance is excellent, and reality meaning in domestic popularization is achieved.

The invention relates to a detection reagent for fast detecting cyanide in water. The detection reagent comprises a release reagent and a color developing reagent, wherein the release reagent comprises monopotassium phosphate, methylcellulose, lactic acid and acetone mixed solvents and chloramine T, the color developing reagent comprises sodium hydroxide, isonicotinic acid, barbituric acid and methylcellulose, and the release reagent and the color developing reagent are encapsulated in a separated way according to the consumption for each time. The release reagent comprises the preparation steps that: 1, major ingredients and deionized water are mixed and ground into paste; 2, the paste is placed in a vacuum drying box for drying; 3, dried materials are cooled and ground into powder; 4, the lactic acid and acetone mixed solvents are used for preparing 5% chloramine T solution; and 5, the chloramine T solution is added into the prepared powder, the materials are dried in the air, and the powdery release reagent is obtained. The preparation steps of the release reagent refer to the partial steps. The detection reagent disclosed by the invention can be matched with a colorimetric tube and a standard colorimetric card to be used, the carrying is convenient, the detection range is wide, the time is short, and convenience can be provided for the fast detection of the cyanide in water in various sites.

The disclosed method comprises: detecting electrolyte with flame reaction, detecting anion with Cl- and CO3(2-); using diluted HCl and KCNS solution to detect iron element and its existing form; igniting in crucible, then reacting with CuO to detect the carbon power. This invention is simple and fast.

The invention discloses a detection process for a high-precision elbow. The detection process is characterized in that the detection process comprises the following requirements that: (1) the elbow appearance has no defects such as cracks, delamination, wrinkles and over-burning, (2) the elbow wall thickness reduction shall be smaller than 10% of the thickness, and the measured thickness shall notbe smaller than a designed calculation thickness, (3) the ellipticity of a curved portion shall be smaller than 1% of a nominal diameter, (4) the deviation of the slopes of two end faces of the elbowshall be smaller than 1% of the outer diameter of a steel pipe and shall be not larger than 1.5mm, and (5) the bending angle error of the elbow shall not exceed + / -1 degree. The detection process hasthe advantages of reasonable process, low cost, reasonable and coordinated match of various parameters, short time and simple operation.

The invention discloses a method for detecting thrombin by using nanogold. According to the method, two oligonucleotide sequences are designed and are respectively marked as TB-Apt1 and TB-cApt1, the TB-Apt1 is a thrombin nucleic acid aptamer, and the TB-cApt1 is a complementary chain of the thrombin nucleic acid aptamer. The oligonucleotides are anchored on the surface of the nanogold through a plurality of adenine fragments respectively. And the TB-Apt1 and the TB-cApt1 are hybridized with each other, so that the nanogold is agglomerated. After the thrombin is added, the TB-Apt1 is combined with the thrombin, a hybrid chain formed by the TB-Apt1 and the TB-cApt1 is separated, and the nanogold is changed into a dispersed state from an agglomerated state. The degree of the dispersion state of the nanogold in the solution is in positive correlation with the thrombin concentration. And the thrombin concentration can be calculated by observing the absorption light spectrum intensity of the nanogold solution. According to the method, the surface of the nanogold is modified with oligonucleotide by using an adenine fragment, so that the cost of nucleic acid functionalized nanogold is reduced, low-concentration detection of thrombin is realized in the process of inducing the nanogold from agglomeration to depolymerization by using thrombin, and the sensitivity of the detection method is improved.