50results about How to "Detection economy" patented technology

The invention discloses a myoglobin assay kit. The myoglobin assay kit is characterized in that the kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is of an appropriate buffer solution; and the reagent R2 is formed by placing myoglobin antibody-sensitized polystyrene latex particles in the appropriate buffer solution. The myoglobin assay kit has the advantages of simple and fast detection, high sensitivity, good accuracy, strong anti-interference ability and low production cost.

The invention discloses a full-range C-reactive protein detection kit, which comprises a reagent R1 and a reagent R2. The reagent R1 is a proper buffer solution; the reagent R2 is formed by the following steps: sensitizing two or more styrene latex with different average grain diameters by using an anti-human C-reactive protein antibody; placing the sensitized styrene latex with the different average grain diameters into the proper buffer solution respectively to form reagents; and finally mixing the reagents in different proportions, wherein the reagent R2 contains 0.8 to 3.5 mg / mL of the styrene latex combined with the anti-human C-reactive protein antibody. The full-range C-reactive protein detection kit not only can measure lower CRP content but also can measure high CRP content, and has the advantages of high sensitivity and stability and accurate measurement.

The invention relates to a pillararene modification cyanide ion fluorescence detection test paper and a preparation method thereof and a detection method thereof. The cyanide ion fluorescence detection test paper is oxidized by the filter paper and placed in the pillararene solution for immersion treatment, then immersed in the sodium borohydride solution after washing, washed, dried, and then immersed in the 10-methyl acridine solution, after cleaning and drying, cyanide ions used for detecting the 10-3~10-7M concentration range are obtained. The method is advantaged in that the cyanide ion fluorescence detection test paper is used for detecting the cyanide ion concentration in the solution, the to-be-detected solution is dropped to the test paper for detection, through observing change of fluorescence intensity on the test paper, the cyanide ion concentration can be quantitatively detected through naked eyes, the response speed is fast, the result can be rapidly determined, and the cyanide ion concentration in the solution can be simply, conveniently, rapidly and economically detected.

The invention provides a nucleic acid aptamer-based lung metastatic osteosarcoma tissue immunofluorescence kit. The nucleic acid aptamer-based lung metastatic osteosarcoma tissue immunofluorescence kit comprises a), an antigen repairing liquid; b), a washing liquid; c), a nucleic acid aptamer dilution liquid; d), a blocking liquid. In applications, Cy5 labelled nucleic acid aptamer is taken as a fluorescence probe for labeling of paraffin embedded lung metastatic osteosarcoma tissue. The nucleic acid aptamer is capable of realizing specific combination with lung metastatic osteosarcoma cells,and combination with other cells is impossible to realize, so that the nucleic acid aptamer is taken as a novel molecule probe with high specific recognition capacity on lung metastatic osteosarcoma,and possesses important research meaning in early stage diagnosis and treatment of osteosarcoma susceptible to pulmonary metastasis. In applications, paraffin section immunofluorescence dyeing is adopted, pathological diagnosis on lung metastatic osteosarcoma tissue can be realized without low temperature storage, the specificity is high, the sensitivity is high, operation is simple, equipment requirement is low, positioning performance is excellent, and reality meaning in domestic popularization is achieved.

The invention discloses a room-temperature phosphorescence detection method of lysozyme and an application, belongs to the technical field of detection of lysozyme, and can solve the problems of complex detection process, high cost and strong interference of lysozyme at present. A prepared Mn:ZnS room-temperature phosphorescence quantum dot modified with beta-cyclodextrin is used as a phosphorescence probe, a lysozyme nucleic acid aptamer is used as a molecular recognition unit, and analysis and detection of the lysozyme can be realized by detecting the room-temperature phosphorescence intensity of a system after the lysozyme is added. The response range of the phosphorescence detection system to the lysozyme is 5.5 nM-44.4 nM and the detection limit is 0.54 nM. The method can be used fordetecting lysozyme in serum, urine samples, honey and wine, and the complex sample pretreatment process during detection can be avoided. A deoxidant and an inducer are not required to be added for production of room-temperature phosphorescence, and interference of background fluorescence and scattered light of actual samples can be avoided.

The invention relates to a detection reagent for fast detecting cyanide in water. The detection reagent comprises a release reagent and a color developing reagent, wherein the release reagent comprises monopotassium phosphate, methylcellulose, lactic acid and acetone mixed solvents and chloramine T, the color developing reagent comprises sodium hydroxide, isonicotinic acid, barbituric acid and methylcellulose, and the release reagent and the color developing reagent are encapsulated in a separated way according to the consumption for each time. The release reagent comprises the preparation steps that: 1, major ingredients and deionized water are mixed and ground into paste; 2, the paste is placed in a vacuum drying box for drying; 3, dried materials are cooled and ground into powder; 4, the lactic acid and acetone mixed solvents are used for preparing 5% chloramine T solution; and 5, the chloramine T solution is added into the prepared powder, the materials are dried in the air, and the powdery release reagent is obtained. The preparation steps of the release reagent refer to the partial steps. The detection reagent disclosed by the invention can be matched with a colorimetric tube and a standard colorimetric card to be used, the carrying is convenient, the detection range is wide, the time is short, and convenience can be provided for the fast detection of the cyanide in water in various sites.