50results about How to "Detection economy" patented technology

The invention relates to a nondestructive testing method of a concrete structure, which comprises the steps: based on the original infrared imaging system, an ultrasonic excitation source is additionally arranged on a tested object; after the ultrasonic excitation source is used, infrared pulse excitation continues to be adopted for combining ultrasound damage location with infrared depth analysis, which protects the tested object, avoids detection of the whole thermography area, aims at detection of the determined image processing area, and is beneficial to improving the detection efficiency; and then, a standard part is adopted for sample detection, and the depth information of the tested object is reversely deduced. The nondestructive testing method is feasible, and has low requirement on precision of detection apparatuses.

The invention discloses a retinol conjugated protein detection kit. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 is a buffer solution, the reagent R2 is the mixture of retinol conjugated protein antibody sensitized polystyrene latex particles and the buffer solution. The retinol conjugated protein detection kit has the advantages that the kit is simple and quick to detect, high in sensitivity, good in accuracy, good in anti-interference capacity and low in production cost; a lipoprotein a (retinol conjugated protein) detection method adopted by the kit is a latex enhanced immuno-turbidimetry and enables the lipoprotein a (retinol conjugated protein) detection to be more economical, convenient and quicker, and the retinol conjugated protein detection kit is applicable to automatic biochemical analyzers of most of hospitals and especially can achieve quick quantitative detection for emergency treatment.

The invention relates to a urine microalbumin detection kit and a preparation method thereof. The detection kit is prepared from a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from buffer solution (1), inorganic salt ions (1), accelerator (1), stabilizer (1) and preservative (1), and the reagent R2 is prepared from buffer solution (2), inorganic salt ions (2), preservative (2), stabilizer (2), suspending aid (1) and anti-human albumin antibody. The preparation method of the detection kit disclosed by the invention comprises the steps of preparing reagents according to constituent content; mixing a sample to be tested with the reagent R1 and the reagent R2 to enable the sample, the reagent R1 and the reagent R2 fully react; utilizing a full-automatic biochemical analyzer to measure an absorbance difference value after reaction; calculating out the concentration of urine microalbumin in the sample according to an absorbance change value. The detection kit disclosed by the invention has the advantages of low equipment cost, high sensibility, good accuracy and precision, easiness to store, good data repeatability and wide application to clinical biochemical analyzers.

The invention relates to a test tube test method specially aimed at the genetic toxicity effect of chemical pollutants, which belongs to the technical category of environmental detection and evaluation. Design and invent a special test tube for SOS/umu genotoxicity detection, use the test tube as a container for bacterial culture, toxicity exposure, damage repair, color development, and test, use a pipette (or pipette) to transfer the solution, bacteria The solution was mixed and oscillated by a constant temperature oscillator in a water bath, and the absorbance was measured by a visible spectrophotometer. The simple, economical, green and accurate detection of chemical pollutants and genotoxic effects of environmental samples is realized, and it is also convenient for the promotion and popularization of the method.

The invention provides a hybridoma cell strain capable of secreting a T-2 toxin monoclonal antibody and also provides the T-2 toxin monoclonal antibody secreted by the cell. The monoclonal antibody has the ascite potency ratio of 1:50000 which is superior to that of the existing monoclonal antibody. Meanwhile, the cross reaction rate of the monoclonal antibody and HT-2 toxin reaches up to 92.8%, and the cross reaction rate of the monoclonal antibody and various other antigens is lower than 0.1%. An immunoaffinity column-liquid chromatography containing the monoclonal antibody can be used for simultaneously and efficiently separating and extracting T-2 toxin and HT-2 toxin in samples and is accurate, economic and effective.

The invention belongs to the technical field of tetracycline detection, particularly relates to application of a sulfur quantum dot as a fluorescent probe in tetracycline detection, and provides new application of the sulfur quantum dot in tetracycline detection in order to establish an economic, rapid, sensitive and high-selectivity tetracycline detection method, namely application of the sulfur quantum dot as the fluorescent probe in tetracycline detection, the characteristic that the detection of the tetracycline by the sulfur quantum dot has relatively high selectivity is utilized, the tetracycline concentration by the sulfur quantum dot is taken as a fluorescent probe, the tetracycline concentration in a to-be-detected sample can be measured according to the linear relation between the fluorescence intensity ratio of the sulfur quantum dot fluorescence before and after the tetracycline to-be-detected sample is added, and the detection process is rapid and sensitive. Therefore, the defects of long detection time, low sensitivity and the like in a traditional tetracycline detection technology are overcome, economic, rapid and sensitive detection of tetracycline antibiotics is realized, and the sulfur quantum dot has huge application potential in rapid and sensitive detection of food matrix tetracycline.

The invention relates to a method for detecting material and capacity of a distribution transformer winding, which orderly comprises the following steps: step 1, counting the distribution of a material parameter and a capacity parameter of the winding; step 2, dividing a distribution interval; step 3, determining core data; step 4, dividing the risk grade, and step 5, judging whether the distribution transformer has a problem. The method for detecting material and capacity of the distribution transformer winding in the invention has the following advantages: through the detection method, the distribution transformer of the suspected winding material or the capacity faking problem in large-scale distribution transformer can be primarily selected quickly; then, the inspection personnel confirms the distribution transformers having the suspected problem one by one, thereby ensuring stable work of the distribution transformer and ensuring the safety performance of the whole power distribution system; the whole detection process is economic, reliable in result and short for the period; the detection means is intelligent and efficient; different core data are set to adapt to different detection targets, thereby meeting different detection requirements.

The invention discloses a method for detecting the Fenghua21 watermelon seed authenticity and purity. The method comprises the following steps that 1, polymorphic stable SSR primers in Fenghua21 maleparents, female parents and hybrids are screened out; 2, an SSR fingerprint spectrum of Fenghua21 is prepared; 3, SSR amplification band patterns of to-be-detected watermelon seeds are compared with the Fenghua21 SSR fingerprint spectrum prepared in step 2, and if comparison results are consistent, the to-be-detected watermelon seeds are judged as true Fenghua21 watermelon hybrid seeds. The methodfor detecting the Fenghua21 watermelon seed authenticity and purity has the advantages that the method is not affected by an external environment, detection can be completed indoors, and watermelon hybrids which are closely related can be identified, so that the method for detecting the Fenghua21 watermelon seed authenticity and purity is accurate, reliable, fast, convenient and economic.

The invention belongs to the field of immunological detection and particularly relates to a method for evaluating the blocking activity of an immune checkpoint molecular blocking antibody by magneticbeads. Magnetic beads with solid-phase anti-tag molecular antibodies and immune checkpoint molecular protein expressing label molecules (label I) subjected to solid-phase on the surfaces of the magnetic beads are used; the magnetic beads can be specifically bound with a ligand of an immune checkpoint molecule with another label molecule (label II), and then a label II specific binding antibody labeled by a biologic substance (fluorescent or visible light) is used for quantitatively detecting the immune checkpoint molecular protein/ligand molecular protein compound on the surface of the magnetic bead. When the blocking antibody of the immune checkpoint molecule, the magnetic beads with the immune checkpoint molecule protein in the solid phase and the ligand protein coexist, the blocking activity of the antibody is inversely proportional to the final luminous intensity of the magnetic beads, so that quantitative analysis on the blocking activity of the antibody is realized through lightintensity detection. The magnetic bead method is simple to operate, quick in reaction, economical and time-saving.

The invention relates to a dioxin detection device and device method thereof. The method is based on biological affinity and comprises the following steps: contacting the detection device with the sample containing dioxin or dioxin-like material; generating a screening effect on the electrode surface using a biological identification element on the detection device and the affinity between the dioxin or dioxin-like material; measuring the current signal difference between the post-testing current signal and the before-testing current signal and comparing the standard solution containing the dioxin or dioxin-like material with the corresponding current signal difference, thereby determining the concentration of the dioxin or dioxin-like material in sample.

The invention provides a method for rapidly determining the content of mercaptoacetic acid. The method comprises the following steps: oxidizing sulfydryl by iodine under an alkaline condition; limiting the reaction speed through IO3<->generated by reaction; and determining the titration end point to obtain the content of mercaptoacetic acid. According to the method for rapidly determining the content of mercaptoacetic acid, the recovery rate of a mercaptoacetic acid titration method reaches 98%; meanwhile, the method is stable.

The invention provides an iodide ion recognition probe and a preparation method thereof. The preparation method comprises the following steps: reacting 9,9-bis(6-bromohexyl)-fluorene-2,7-dicarbaldehyde serving as a raw material with 2,5-diaminopyridine under the condition of anhydrous LiCl serving as a catalyst to obtain an intermediate product; and under the condition that N,N-dimethylacetamide is taken as a solvent, reacting the intermediate product with trimethylamine to obtain a pyridine fluorenyl fluorescent conjugated polymer. The synthesis method is simple, industrial production is easy, iodine ions can be rapidly, sensitively, accurately and economically detected, the efficiency is high in an actual application process, and the probe and method are particularly suitable for detection of micro and trace iodine ions. The fluorescence probe disclosed by the invention is high in iodine ion detection selectivity, the lowest detection limit is 4.68*10<-7> mol/L, a good linear quantitative relation is shown in a range of 0-25 [mu]mol/L, and the fluorescence probe is suitable for trace detection.

The invention provides a system and a method for detecting a heat treatment state of a high-steel-grade thick-wall pipe fitting based on deep learning. The method comprises the following steps: 1, acquiring metallographic structure images of the inner surface and the outer surface of a to-be-detected area of the high-steel-grade thick-wall pipe fitting; 2, establishing a data set, and dividing the data set into a training set and a test set; 3, constructing a convolutional neural network, and training the constructed convolutional neural network to obtain a trained convolutional neural network; predicting the trained convolutional neural network, and classifying prediction results to obtain a classification result graph; 4, predicting the to-be-detected high-steel-grade thick-wall pipe fitting subjected to heat treatment, judging a prediction result, and further judging whether the to-be-detected high-steel-grade thick-wall pipe fitting subjected to heat treatment is qualified or not; the aim of economically, efficiently, objectively and accurately inspecting the heat treatment state of the high-steel-grade rear wall pipe fitting is achieved.

The disclosed method comprises: detecting electrolyte with flame reaction, detecting anion with Cl- and CO3(2-); using diluted HCl and KCNS solution to detect iron element and its existing form; igniting in crucible, then reacting with CuO to detect the carbon power. This invention is simple and fast.

The invention discloses a detection process for a high-precision elbow. The detection process is characterized in that the detection process comprises the following requirements that: (1) the elbow appearance has no defects such as cracks, delamination, wrinkles and over-burning, (2) the elbow wall thickness reduction shall be smaller than 10% of the thickness, and the measured thickness shall notbe smaller than a designed calculation thickness, (3) the ellipticity of a curved portion shall be smaller than 1% of a nominal diameter, (4) the deviation of the slopes of two end faces of the elbowshall be smaller than 1% of the outer diameter of a steel pipe and shall be not larger than 1.5mm, and (5) the bending angle error of the elbow shall not exceed +/-1 degree. The detection process hasthe advantages of reasonable process, low cost, reasonable and coordinated match of various parameters, short time and simple operation.