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Method for long-term preservation and rapid recovery of luminous bacterium strain

A technology of luminescent bacteria and strains, applied in the field of environmental detection and evaluation, can solve the problems of high cost, waste, and short effective storage time, and achieve the effects of reducing experimental costs, long storage time, and shortening the time of toxicity testing

Inactive Publication Date: 2012-05-23
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

For the lyophilized powder, each toxicity test needs to use a lyophilized powder, which is costly and wasteful, and sometimes the lyophilized powder is not bright enough to carry out the toxicity test after the recovery of the lyophilized powder, and it usually needs to be cultivated for several generations before it can be used. Toxicity test; For bacteria stored on solid slant medium, experimental operators often need to have a certain technical level, and the effective storage time is relatively short

Method used

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  • Method for long-term preservation and rapid recovery of luminous bacterium strain

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Embodiment

[0011] Divide 250 ml of the bacteria liquid that has been cultured for several generations into six sterilized 50 mL centrifuge tubes, centrifuge at 4°C and 3000 rpm for 30 minutes, discard the supernatant, and keep the bacteria at the bottom. Add 50ml of cryopreservation medium that has been sterilized by high temperature into each centrifuge tube, mix well, and divide into 1.5mL sterile cryopreservation tubes under aseptic conditions, and place the subpackaged cryopreservation tubes at -20 ℃ refrigerator. After storage for a period of time, take out a cryopreservation tube, thaw it quickly, centrifuge at 4000 rpm for 10 minutes at 4°C, discard the supernatant, add 1.0mL resuscitation solution, mix well, and then centrifuge at 4000 rpm Centrifuge at the speed of 10 minutes, discard the supernatant, keep the bacteria at the bottom, add 1.0mL of resuscitation solution to the centrifuge tube again, mix well, carefully transfer the mixture into 100mL of resuscitation solution, an...

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Abstract

The invention relates to a method for long-term preservation and rapid recovery of luminous bacterium strain, and belongs to the technical field of environmental detection and evaluation. Through exploitation of a special bacterium freeze-stored medium and a recovery solution, the method provided by the invention realizes long-term preservation and rapid recovery of luminous bacterium strain, saves a testing cost, greatly shortens toxicity testing time, and provides possibility for chemical pollutant toxicity rapid testing. The method is simple and economic, can produce effects fast, prolongs strain preservation time, allows a low experimental operation technology level, and is convenient for popularization and utilization.

Description

technical field [0001] The invention relates to a preservation and recovery method specially aimed at luminescent bacterial strains, and belongs to the technical category of environmental detection and evaluation. Background technique [0002] With the rapid development of social economy and the intensification of industrial and agricultural production activities, more and more chemicals are produced and used. According to reports, the number of registered chemicals exceeds tens of millions. Daily life and production The number of chemicals used in more than 100,000 species. These chemicals may enter the environment or remain in the human body and organisms through various channels during the production and use process, posing a serious threat to the ecological environment and human health. In order to accurately evaluate the safety of chemicals and environmental samples, biological toxicity effects are essential and the most important basic data. [0003] There are dozens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/20C12R1/01C12R1/63
Inventor 魏东斌谭卓威杜宇国
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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