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Application of sulfur quantum dot as fluorescent probe in tetracycline detection

A Fluorescent Probe, Sulfur Quantum Technology

Inactive Publication Date: 2021-09-14
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no application of using sulfur quantum dots as a fluorescent probe to detect tetracyclines.

Method used

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  • Application of sulfur quantum dot as fluorescent probe in tetracycline detection
  • Application of sulfur quantum dot as fluorescent probe in tetracycline detection
  • Application of sulfur quantum dot as fluorescent probe in tetracycline detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 sulfur quantum dot fluorescent probe

[0033] (1) Add 50mL of double distilled water into a 100mL round bottom flask, add 4.0g of sodium hydroxide and stir until completely dissolved, then add 3.0mL of polyethylene glycol 400, and finally add 1.4g of sublimated sulfur, stir to dissolve.

[0034] (2) The round bottom flask was placed in an oil bath, heated to 70°C at a stirring speed of 1200rpm, and then stirred and reacted at a stirring speed of 500rpm at 70°C for 72h to obtain a dark yellow sulfur quantum dot solution.

[0035] (3) After naturally cooling to room temperature, the H 2 o 2 Solution (30wt%) was quickly added in the resulting solution with a volume ratio of 2:5 (H 2 o 2 Solution: sulfur quantum dot solution=2:5), and continue to stir the mixture at this stirring speed for 30min; subsequently, the obtained light yellow solution is purified through a dialysis bag (500Da), and the obtained solution is heated at 45°C and 80rp...

Embodiment 2

[0037] Example 2 Fluorescence response of sulfur quantum dot fluorescent probes to three types of substances (metal ions, biomolecules and tetracyclines)

[0038] This example provides the fluorescent response of the sulfur quantum dot fluorescent probe of embodiment 1 to three types of substances, these three types of substances include common metal ions (Al 3+ 、Cr 3+ 、Cu 2+ , Hg 2+ 、Co 2+ , Pb 2+ 、Cd 2+ 、Ni 2+ 、Ba 2+ , Fe 3+ ), biomolecules [cysteine ​​(Cys), histidine (His), glycine (Gly), phenylalanine (Phe), arginine (Arg), lysine (Lys), tyrosine (Tyr) glutathione (GSH), dopamine (DA), uric acid (UA), ascorbic acid (AA), vitamin B1 (VB1), glucose (Glu)] and tetracycline (TC).

[0039] The specific test includes the following steps:

[0040] (1) get the nitrate of certain quality above-mentioned metal ion, be mixed with the metal ion solution of 0.01mol / L respectively;

[0041] (2) Take a certain mass of the above-mentioned biomolecules, and prepare 0.1mol / L amino...

Embodiment 3

[0048] Example 3 A method for the detection of tetracycline in milk samples by a sulfur quantum dot fluorescent probe

[0049] The concrete steps of the detection method of this example are as follows:

[0050] (1) Pre-determination of milk samples purchased from supermarkets: add 5 mL of 1% trichloroacetic acid solution to 5 mL of milk samples and mix well, then sonicate for 10 min and centrifuge at 12000 rpm for 5 min to remove protein, then use Filter the supernatant with a 0.22 μm filter membrane to remove fat, and obtain the milk sample solution to be tested;

[0051] (2) Accurately weigh 0.0481g of tetracycline hydrochloride powder, dissolve it in a 50mL beaker and transfer it to a 100mL volumetric flask, set the volume to 100mL to prepare a 0.001mol / L tetracycline hydrochloride solution, and take a certain amount of 0.001mol / L tetracycline solution is diluted to prepare 0.0001mol / L and 0.00001mol / L tetracycline solution;

[0052] (3) Add different amounts of the abov...

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Abstract

The invention belongs to the technical field of tetracycline detection, particularly relates to application of a sulfur quantum dot as a fluorescent probe in tetracycline detection, and provides new application of the sulfur quantum dot in tetracycline detection in order to establish an economic, rapid, sensitive and high-selectivity tetracycline detection method, namely application of the sulfur quantum dot as the fluorescent probe in tetracycline detection, the characteristic that the detection of the tetracycline by the sulfur quantum dot has relatively high selectivity is utilized, the tetracycline concentration by the sulfur quantum dot is taken as a fluorescent probe, the tetracycline concentration in a to-be-detected sample can be measured according to the linear relation between the fluorescence intensity ratio of the sulfur quantum dot fluorescence before and after the tetracycline to-be-detected sample is added, and the detection process is rapid and sensitive. Therefore, the defects of long detection time, low sensitivity and the like in a traditional tetracycline detection technology are overcome, economic, rapid and sensitive detection of tetracycline antibiotics is realized, and the sulfur quantum dot has huge application potential in rapid and sensitive detection of food matrix tetracycline.

Description

technical field [0001] The invention belongs to the technical field of tetracycline detection, and in particular relates to the application of sulfur quantum dots as fluorescent probes in tetracycline detection. Background technique [0002] Tetracycline (TC) has been widely used to treat bacterial infections in humans and animals due to its broad-spectrum antibacterial activity, low toxicity, low cost, and good oral absorption. In addition, TC is used as a feed additive in animal husbandry due to its ability to promote animal growth. Unfortunately, the long-term human abuse of tetracycline has resulted in its high residue in various environments. These residual tetracyclines will enter the human body through the food chain, causing adverse reactions such as allergies, gastrointestinal disorders, and liver poisoning. In addition, tetracycline can also make bacteria resistant, affecting the use of other antibiotics. Therefore, establishing an effective method to detect tet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643G01N2021/6432
Inventor 甘峰卢海欣
Owner SUN YAT SEN UNIV
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