Method for detecting Fenghua21 watermelon seed authenticity and purity

An authentic and rich technology, applied in the field of molecular biology, can solve the problems of indistinguishable watermelon hybrids, easy to be affected by the environment, labor-intensive and land-consuming, and achieve the effect of abundant quantity, high polymorphism and large quantity.

Active Publication Date: 2018-11-16
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the defects that the purity detection of watermelon seeds in the prior art mostly relies on field phenotype observation, which is easily affected by the environment, has a long cycle, takes up a lot of labor and occupies an area, and is difficult to distinguish between watermelon hybrids with clos

Method used

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  • Method for detecting Fenghua21 watermelon seed authenticity and purity
  • Method for detecting Fenghua21 watermelon seed authenticity and purity
  • Method for detecting Fenghua21 watermelon seed authenticity and purity

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Experimental program
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Embodiment 1

[0058] Embodiment one: a kind of method for detecting the authenticity and purity of 'Fenghua 21' watermelon seeds provided by the invention comprises the following steps:

[0059] (1) Design SSR primers according to the published watermelon genome sequence information, and screen out the polymorphic and stable SSR primers in the parents and hybrids of ‘Fenghua 21’ among the designed SSR primers;

[0060] (2) Use the SSR primers finally screened in step (1) to prepare the SSR fingerprint of 'Fenghua 21';

[0061] (3) The watermelon seeds to be detected are sown, the genomic DNA of the cotyledon stage leaves is extracted by CTAB method, PCR amplification and polyacrylamide gel electrophoresis are carried out using the SSR primers finally screened out in step (1), and the DNA of the seeds to be detected is obtained. The SSR amplified band pattern is compared with the SSR fingerprint of 'Fenghua 21' prepared in step (2). If the comparison results are consistent, it can be determi...

Embodiment 2

[0067] Embodiment two: a kind of method for detecting the authenticity and purity of 'Fenghua 21' watermelon seeds provided by the invention comprises the following steps:

[0068] (1) Design SSR primers according to the published watermelon genome sequence information, and screen out the polymorphic and stable SSR primers in the parents and hybrids of ‘Fenghua 21’ among the designed SSR primers;

[0069] (2) Use the SSR primers finally screened in step (1) to prepare the SSR fingerprint of 'Fenghua 21';

[0070] (3) The watermelon seeds to be detected are sown, the genomic DNA of the cotyledon stage leaves is extracted by CTAB method, PCR amplification and polyacrylamide gel electrophoresis are carried out using the SSR primers finally screened out in step (1), and the DNA of the seeds to be detected is obtained. The SSR amplified band pattern is compared with the SSR fingerprint of 'Fenghua 21' prepared in step (2). If the comparison results are consistent, it can be determi...

Embodiment 3

[0100] Embodiment three: a kind of method for detecting the authenticity and purity of 'Fenghua 21' watermelon seeds provided by the invention comprises the following steps:

[0101] (1) Design SSR primers according to the published watermelon genome sequence information, and screen out the polymorphic and stable SSR primers in the father, mother and hybrid of 'Fenghua 21' among the designed SSR primers;

[0102] (2) Use the SSR primers finally screened in step (1) to prepare the SSR fingerprint of 'Fenghua 21';

[0103] (3) The watermelon seeds to be detected are sown, the genomic DNA of the cotyledon stage leaves is extracted by CTAB method, PCR amplification and polyacrylamide gel electrophoresis are carried out using the SSR primers finally screened out in step (1), and the DNA of the seeds to be detected is obtained. The SSR amplified band pattern is compared with the 'Fenghua 21' SSR fingerprint spectrum prepared in step (2). If the comparison results are consistent, it ...

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Abstract

The invention discloses a method for detecting the Fenghua21 watermelon seed authenticity and purity. The method comprises the following steps that 1, polymorphic stable SSR primers in Fenghua21 maleparents, female parents and hybrids are screened out; 2, an SSR fingerprint spectrum of Fenghua21 is prepared; 3, SSR amplification band patterns of to-be-detected watermelon seeds are compared with the Fenghua21 SSR fingerprint spectrum prepared in step 2, and if comparison results are consistent, the to-be-detected watermelon seeds are judged as true Fenghua21 watermelon hybrid seeds. The methodfor detecting the Fenghua21 watermelon seed authenticity and purity has the advantages that the method is not affected by an external environment, detection can be completed indoors, and watermelon hybrids which are closely related can be identified, so that the method for detecting the Fenghua21 watermelon seed authenticity and purity is accurate, reliable, fast, convenient and economic.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a method for detecting the authenticity and purity of 'Fenghua 21' watermelon seeds. Background technique [0002] Watermelon is an annual vine herb that is currently grown all over the world. Watermelon is rich in glucose, fructose, malic acid, amino acids, lycopene, carotenoids and a variety of vitamins and minerals, while the content of fatty acids and cholesterol is extremely low. It is both nutritious and healthy, and is very popular among consumers. According to the latest statistics from the Ministry of Agriculture, in 2016 my country's watermelon cultivation area was about 28.36 million mu, with a total output of 79.4 million tons, ranking first in the world. It is preliminarily estimated that its annual seed circulation is about 2.8 million kilograms. Seed authenticity and purity inspection is an indispensable and important step in seed production. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6895
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 孙玉燕范敏何艳军牛晓伟
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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