A test tube testing method and device for sos/umu genotoxic effect

A technology of genotoxicity and testing methods, which is applied in the field of environmental pollution detection and evaluation, can solve the problems of disposable experimental consumables, high price, and low penetration rate, and achieve the effects of saving construction costs, high penetration rate, and reducing mass use

Inactive Publication Date: 2011-12-28
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies such as having to use a large number of disposable experimental consumables and expensive, popularized instruments in the traditional SOS / umu genotoxicity detection method, to provide a method using test tubes, pipettes (or single-channel pipettes) liquid device), constant temperature water bath oscillator, visible spectrophotometer and other reusable materials and low-cost, high-pervasive instruments for genotoxicity testing methods, and open up a new path for the promotion of genotoxicity testing methods for chemical pollutants and environmental samples new way

Method used

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  • A test tube testing method and device for sos/umu genotoxic effect
  • A test tube testing method and device for sos/umu genotoxic effect
  • A test tube testing method and device for sos/umu genotoxic effect

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Experimental program
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Embodiment

[0013] Put 0.1 ml of bacteria into a test tube containing 20 ml of culture medium, shake and culture the bacteria overnight at 20°C for 10 hours, keep 0.1 ml of bacteria, add 30 ml of culture medium and different concentrations (0-60 mmol / L) of positive The reference substance 4-NQO solution was shaken and exposed at 20°C for 3.5 hours, 1.0ml of the exposure solution was reserved, 4.0ml of repair solution was added, repaired with shaker at 20°C for 3.5 hours, the absorbance at 595nm was measured and recorded, and 20ml of chromogenic solution was added to the Shake at 20°C for 1.5 hours to develop color, add 10 ml of reaction stop solution, shake at 20°C for 1.5 hours, measure and record the absorbance at 415nm, and substitute it into the formula to calculate the genotoxicity of the positive reference substance.

[0014] I R = A 415 , ...

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Abstract

The invention relates to a test tube test method specially aimed at the genetic toxicity effect of chemical pollutants, which belongs to the technical category of environmental detection and evaluation. Design and invent a special test tube for SOS / umu genotoxicity detection, use the test tube as a container for bacterial culture, toxicity exposure, damage repair, color development, and test, use a pipette (or pipette) to transfer the solution, bacteria The solution was mixed and oscillated by a constant temperature oscillator in a water bath, and the absorbance was measured by a visible spectrophotometer. The simple, economical, green and accurate detection of chemical pollutants and genotoxic effects of environmental samples is realized, and it is also convenient for the promotion and popularization of the method.

Description

technical field [0001] The invention relates to a test tube test method and device for SOS / umu genotoxic effect, belonging to the technical category of environmental pollution detection and evaluation. Background technique [0002] With the rapid development of industrial and agricultural production, environmental problems have become more and more serious. The harm caused by environmental pollution to human beings has far exceeded previous estimates, and the genotoxicity of environmental pollutants has received more widespread attention. Genotoxicity (or genotoxicity) refers to the direct or indirect toxicity of chemical substances and radiation to the genetic material of the body, mainly including gene mutation and chromosomal aberration, both of which are mutations caused by DNA damage. With the rapid development of genetic toxicology-related fields, especially molecular biology techniques, the molecular mechanism of genotoxicity has been elucidated in depth, and methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/03C12Q1/02
Inventor 魏东斌谭卓威杜宇国
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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