404 results about "Signal transduction" patented technology

Disclosed is a system for detecting whether the breast cancer is benign or malignant based on multiple modalities, which comprises: an ultrasonomics model configured to extract breast tumor position information and breast tumor morphological features on the basis of an acquired grayscale breast ultrasound image, perform global interactive fusion on the breast tumor position information and breast tumor morphological feature information, and convert a Transformer encoder-based feature output into an output vector of the corresponding classification dimensions by means of a fully connected layer; a metabonomics model configured to obtain a metabolic fingerprint spectrogram and analyze the metabolic fingerprint spectrogram on the basis of a metabolic database and a predetermined significantly differential metabolite to obtain abnormal metabolic pathways, signal transduction pathways, and related biochemical reactions associated with breast cancer malignancy; and a detection module configured to perform joint modeling, based on the breast tumor position information, the breast tumor morphological features, and a peak value of the significantly differential metabolite of the breast cancer malignancy, to output a prediction result about whether the breast cancer is benign or malignant.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The invention relates to the technical field of agricultural irrigation, in particular to an intelligent biological irrigation system and method based on remote control, and the system comprises a biological sensing unit, a neural computing decision engine, a driving execution unit, and a communication optimization architecture. Wherein the biological sensing unit is used for monitoring multi-modal data of plant physiological status, rhizosphere microenvironment and meteorological parameters in real time; the neural computing decision engine is used for constructing a pulse neural network, fusing a plant dimensional management system signal transduction mechanism and a multi-agent optimization algorithm, and generating a millisecond-level dynamic irrigation strategy; the driving execution unit is used for millimeter-level water quantity adjustment and pipe network self-repairing of the irrigation execution unit; the communication optimization architecture is used for seamless coverage and secure transmission of irrigation data, and meanwhile, a strategy is optimized by applying algorithms such as reinforcement learning and the like. Therefore, the problems of water resource waste, poor irrigation effect, untimely irrigation strategy adjustment and the like in the prior art are solved.

The invention provides a preparation method of a recombinant collagen dressing, and belongs to the technical field of medical dressings. According to the technical scheme, stacked short sequences and continuous human collagen amino acid short sequences are repeatedly combined for multiple times to form recombinant collagen with specificity, and high similarity of the recombinant collagen with human collagen is ensured. On the basis, 4-FPBA is used as a cross-linking agent to respectively form a Schiff base bond responsive to pH and a boric acid ester bond responsive to glucose with CMCS and two kinds of HA with different molecular weights, and then the double-dynamic covalent cross-linked network adhesive hydrogel is synthesized. The triple-helix structure of the recombinant collagen is combined with a specific receptor on a cell membrane, and cell behaviors are regulated and controlled by inducing signal transduction through a cytoskeleton, so that skin microcirculation can be effectively improved, and a skin barrier can be repaired; the hydrogel dressing matrix has the effects of resisting inflammation, resisting oxidation, resisting bacteria, promoting healing and the like, the biological performance of the dressing is remarkably improved, and a better curative effect is achieved.

The invention belongs to the technical field of protein, and relates to a peptide for promoting synthesis of a brain-derived neurotrophic factor (BDNF) as well as a preparation method and application of the peptide. The amino acid sequence of the peptide for promoting synthesis of the brain-derived neurotrophic factor BDNF is SGFSKHF, and the peptide has the main effects that damage of CORT (corticosterone) to PC12 cells can be protected, the peptide can be spontaneously combined with a TrkB receptor, the structure of a compound formed after combination is more stable, BDNF-TrkB-CREB pathway signal transduction can be activated, generation of the BDNF is promoted, and the effect of promoting synthesis of the brain-derived neurotrophic factor BDNF is achieved. Therefore, the occurrence and development of neurodegenerative diseases can be delayed.

The invention discloses an anti-neurodegeneration exosome composition as well as a preparation method and application thereof. The anti-neurodegeneration exosome composition is prepared from the following components in parts by mass: 5 to 25 parts of human exfoliated deciduous tooth stem cell exosome, 25 to 85 parts of plant-derived exosome-like nano-vesicles, 12 to 10 parts of ginsenoside Rg, 2 to 10 parts of curcumin and 10 to 20 parts of auxiliary materials. The anti-neurodegeneration exosome composition contains a large amount of cell factors, growth factors, miRNA, mRNAs and other components, is easy to fuse with cell membranes of intestinal epithelial cells, can selectively deliver bioactive substances to recipient cells through a blood-brain barrier, carries out information transmission among different cells, regulates signal transduction among the cells, and has a good anti-neurodegeneration effect. The effects of resisting oxidation, resisting inflammation, resisting aging and the like are achieved.

The invention discloses an E-CAR-NK cell for treating systemic lupus erythematosus and application of the E-CAR-NK cell, and belongs to the technical field of genetic engineering.A CD19 and BCMA double-target E-CAR is adopted for modifying an NK cell, and the E-CAR-NK cell is obtained; the CD19 and BCMA double-target E-CAR is composed of the following modules: a CD8 signal peptide, a CD19 scFv, a Linker, a BCMA scFv, a CD8 Hinge region, an NKG2D transmembrane region, a CD3 epsilon signal transduction region, a DAP12 costimulatory region, a 2B4 costimulatory region, and a CD3 zeta signal transduction region. Compared with a common CAR-NK cell, the E-CAR-NK cell disclosed by the invention has higher antigen sensitivity and better continuous killing ability, so that the treatment effect of SLE is improved, and the E-CAR-NK cell has lower side effects and higher safety.

The multi-channel electrochemical micro-fluidic chip detection platform provided by the invention realizes rapid, high-throughput, low-cost, high-sensitivity and specific detection on genital tract pathogens. Specifically, when a target exists, a target recognition probe Cas12a-crRNA compound specifically recognizes a target and forms a Cas12a-crRNA-Target triad, the trans-cleavage activity is activated, an activated Cas12a system cuts a G1 / G2 single-chain region in the signal amplification probe, then encapsulated methylene blue is released, the methylene blue further promotes a methyl bromide redox reaction, signal transduction is promoted, and the target recognition probe Cas12a-crRNA compound can be used for recognizing the target. Therefore, high-sensitivity and high-specificity quantitative detection of pathogens is realized. On the basis, through a multi-channel micro-fluidic chip, high-sensitivity detection of multiple targets can be realized at the same time by placing different crRNA sequences in reaction tanks respectively. In the embodiment of the invention, the multi-target ultra-sensitive detection of CT, CA, TV and NG is successfully realized, and the detection limit is as low as 0.8 fM.

Provided are a recombinant human interleukin-11 mutant, and a conjugate of the mutant conjugated to a chemical molecule and a use thereof. The recombinant human interleukin-11 mutant achieves soluble expression in an Escherichia coli expression system by means of an SUMO sequence tandem fusion method. The conjugate is obtained by the site-specific conjugation of the mutant to the chemical molecule at a mutant amino acid residue in position W147 by means of a covalent bond. The chemical molecule is at least one of polyethylene glycol, an alkanoic acid aliphatic chain, and a hydrophilic high-molecular polymer containing an alkanoic acid aliphatic chain. The conjugate retains the binding capacity to human interleukin-11 receptor A while demonstrating significantly reduced binding capacity to human IL-6 signal transducer receptor. The conjugate shows the ability to block the signaling between human IL-11 and IL-11 RA and between human IL-11 and GP130 either in vivo or in vitro and can be used for the treatment of associated diseases with tissue fibrosis as a clinical manifestation caused by elevated primary or secondary IL-11.

The invention discloses a preparation method and application of a novel hybrid extracellular vesicle. According to the invention, vesicles derived from endothelial cells and neutrophil cells are combined with deferoxamine, and a biological mixed nano vesicle platform (DFO (HEVS)) is developed to solve the problem of diabetes wound healing. According to the dual-targeting system, DFO is accurately delivered to a wound part by utilizing CXCR4 mediated endothelial cell homing and beta2 integrin dependent inflammation tropism, DFO (at) HEVs activates and recovers vascular regeneration through HIF-1alpha / VEGF, ferroptosis is inhibited through Nrf2 / GPX4 signal transduction, macrophages are reprogrammed into a repair promoting M2 phenotype, and oxidative stress-inflammation-ferroptosis circulation is effectively broken.

The invention provides a naked eye visual detection method based on CRISPR / Cas reaction system nucleic acid detection and a kit thereof, in the method, a solution of a CRISPR / Cas reaction system is prepared, the solution contains a Cas enzyme with trans-cleavage ability, crRNA and an SSA capture probe, the SSA capture probe is constructed as a recognition element by combining a CRISPR / Cas signal amplification system, and the SSA capture probe is used for detecting nucleic acid in the CRISPR / Cas reaction system. An artificially programmed CG probe with a color developing function, which specifically responds to an SSA probe, is used as a signal transduction element, the SSA probe is used for effectively capturing the CG probe, target nucleic acid is used for activating the activity of CRISPR / Cas trans-cleavage, and the SSA and the CG probe are cleaved, so that colloidal gold is in a free state in a solution, and after magnetic separation is observed by naked eyes, the CG probe and the SSA probe are separated by magnetic force. When the detected sample is positive, the supernatant color is pink, the supernatant of the negative sample and the supernatant of the blank sample are in a colorless state, and the target nucleic acid is detected by distinguishing the colors.

Methods for interfering or controlling IFN induction, IFN production and / or IFN activity are disclosed, harnessing viral mechanisms for evading or suppressing host anti-viral immune reactions. These methods comprise administration of one or more viral messenger RNAs (mRNAs) coding for proteins or peptides that control or interfere with signal transduction upstream and / or downstream of IFN induction, thereby providing a means for preventing or treating diseases, disorders or conditions associated with increased IFN production, such as cancer, autoimmune diseases, inflammatory diseases and interferonopathies.

The invention discloses a method for screening a Wilson disease serum exosome miRNA biomarker based on a transcriptomics technology, and belongs to the field of bioinformatics analysis of diseases. According to the invention, the effect of miRNA in WD pathogenesis is discussed by identifying serum exosome miRNA, and a potential biomarker is provided for accurate diagnosis and treatment of the disease. According to the invention, 59 DE-miRNAs are identified by analyzing the expression of differential miRNAs of WD and control group patients, and the DE-miRNAs are related to key approaches such as metabolic regulation, cancer progression, signal transduction and the like. Besides, the reliability of the key DE-miRNA, including miR-451a, miR-204-5p and the like, is confirmed through experimental verification, and the potential of the key DE-miRNA as a non-invasive biomarker is indicated. Research results provide important theoretical basis and new insight for accurate diagnosis of WD and development of potential therapeutic targets.

The invention discloses an antibody combined with interleukin 4 receptor (IL-4R) protein and a computer optimization method, a compound structure analysis method and application thereof, the antibody comprises a Fa-2 sequence, a Fa-3 sequence, a Fa-4 sequence, a Fa-5 sequence, a Fa-7 sequence, a Fa-13 sequence, a Fa-15 sequence, a Fa-16 sequence, a Fa-17 sequence, a Fa-19 sequence and a Fa-23 sequence, and the Fa-2 sequence, the Fa-3 sequence, the Fa-4 sequence, the Fa-5 sequence, the Fa-7 sequence, the Fa-13 sequence, the Fa-15 sequence, the Fa-16 sequence, the Fa-17 sequence, the Fa-19 sequence and the Fa-23 sequence comprise a heavy chain variable region and a light chain variable region. The amino acid sequences of the amino acid sequences are respectively shown as SEQ ID NO: 7, 8, 9, 10, 11, 12, 13, 14, 17, 18, 29, 30, 33, 34, 35, 36, 37, 38, 41, 42, 49 and 50. According to the invention, a brand-new IL-4R epitope is found, and an antibody with a'non-ligand blocking 'characteristic is obtained for the first time. The invention opens up a brand new path for developing a therapy for intervening the IL-4R pathway through a new mechanism of regulating signal transduction or interacting with other coreceptors and the like, and is expected to overcome the limitation of the existing therapy. According to the method, computational biology and experimental structure biology are seamlessly connected, a set of repeatable and efficient research and development process is formed, and the method has a wide application prospect.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.

The invention discloses a group of blocking monoclonal antibodies of an SH1 homologous cluster fully human thyrotropin receptor (TSHR) (a TSH receptor, TSHR) as well as a preparation method and application of the blocking monoclonal antibodies of the SH1 homologous cluster fully human thyrotropin receptor (TSHR). The method comprises the following steps: sorting plasma cells and memory single B cells for specifically recognizing TSHR in peripheral blood of a patient with high TSH receptor blocking antibody (TBAb) titer by using flow cytometry, cloning antibody light and heavy chains in vitro and performing recombinant expression, and performing antibody property screening verification by using hTSHR-CHO cells. The blocking monoclonal antibody specifically targeting the human TSHR is obtained. The fully human TSH receptor blocking monoclonal antibody disclosed by the invention can be specifically combined with TSHR, and can effectively block signal transduction after the TSH is combined with the receptor; the synthesis and secretion of thyroid hormone are inhibited; expression and fibrosis of effector cell orbital fibroblasts TSHR for inhibiting thyroid-related eye diseases are remarkably relieved, and wide application prospects are achieved in treatment of Graves' diseases and diseases such as thyroid eye diseases caused by hyperthyroidism.

The invention discloses a hydrogen peroxide sensing electrode, a preparation method thereof, a hydrogen peroxide electrochemical sensor and a monitoring method. The hydrogen peroxide sensing electrode comprises a tube body, the tube body is provided with a first end and a second end, the first end is provided with a first opening, the second end is a tip, the tip is provided with a second opening, the first opening is communicated with the second opening, and the caliber of the second opening is smaller than that of the first opening; the tip is filled with solid hydrogel embedded with catalase, and the aperture of the solid hydrogel is 20-50 nm; the tube body is filled with an electrolyte, and the electrolyte is a solution with ionic conductivity; the electrolyte is in contact with the solid hydrogel. According to the invention, nano confinement hydrogel and electrophoresis-like technologies are combined, oxygen nano bubbles are used as a signal transduction mechanism, ultrasensitive detection of intracellular H2O2 is realized through ionization sensing, and dynamic change of intracellular hydrogen peroxide can be monitored in situ in real time.

The invention provides a pathogen viable bacteria detection kit based on endogenous protein activated deoxyribozyme. The kit comprises a deoxyribozyme recognition element and a signal transduction element. The deoxyribozyme recognition element is composed of a substrate chain and an enzyme chain, and the signal transduction element is composed of two DNA reporter probes (Sub-F and Sub-Q), wherein the tail ends of the two DNA reporter probes are respectively marked with a fluorescence staining group and a fluorescence quenching group. The deoxyribozyme recognition element can recognize specific endogenous protein of target pathogenic viable bacteria and activate an enzyme chain to cut a substrate chain to generate two independent substrate chain fragments. And then a signal transduction element is introduced to realize specific output of a fluorescence signal. The kit provided by the invention can realize detection of living salmonella by targeting endogenous protein, can sensitively detect 190 CFU / mL of living salmonella with abundance as low as 0.1%, and has a good application prospect in the directions of food safety detection and clinical examination.

Described in several example embodiments herein are engineered programmable pattern recognition compositions and uses thereof. In an embodiment, the engineered protein contains an NTPase of a Signal Transduction ATPases with Numerous-associated Domains (STAND) superfamily (STAND NTPase), comprising a pathogen-associated molecular pattern (PAMP) recognition activity, wherein the STAND NTPase and the PAMP recognition activity are derived from the same or different prokaryotes.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a GhHAT18 gene in regulation and control of drought stress resistance of plants, the nucleotide sequence of the GhHAT18 gene is shown as SEQ ID NO.1. The invention discloses that the cotton histone acetylase gene GhHAT18 can be used for regulating and controlling the drought resistance of the plants for the first time, and experiments show that compared with a wild type plant, after the expression of the GhHAT18 gene is inhibited, the GhHAT18 gene can be used for regulating and controlling the drought stress resistance of the plants. The antioxidant enzyme activity and the drought resistance of plants can be obviously reduced; and the transgenic plant over-expressed with the GhHAT18 shows stronger and higher antioxidant enzyme activity and drought resistance under the drought stress. Transcriptome analysis shows that the GhHAT18 gene affects the expression of drought-resistant related genes through a secondary metabolite synthesis pathway and an MAPK signal transduction pathway, and the adaptive capacity of plants to drought stress is further regulated and controlled.

The invention belongs to the technical field of genetic engineering and biological medicine, and particularly discloses a tumor stem cell targeting plasmid based on a logic gating circuit, a nano-carrier and a preparation method and application of the tumor stem cell targeting plasmid and the nano-carrier. The tumor stem cell targeting plasmid provided by the invention comprises a first plasmid pGal4 and a second plasmid pUAS, a logic AND gate circuit based on protein degradation is designed by utilizing two transcription factors Sox4 and Maff specifically expressed by liver cancer stem cells, and only the liver cancer stem cells with Sox4 and Maff simultaneously highly expressed can express stable target protein GAL4; meanwhile, a target protein GAL4 of an AND gate circuit is set as a primary signal by utilizing a cascade amplification principle of signal transduction, and the signal is used for driving expression (secondary signal) of downstream GSDMD-N and immune activation factors and improving the expression quantity of GSDMD-N in liver cancer stem cells, so that specific pyroptosis of tumor stem cells is induced, and the effect of cancer immunotherapy is enhanced.

The invention discloses a group of blocking monoclonal antibodies of a CHK36 homologous cluster fully human TSH receptor (TSHR) as well as a preparation method and application of the blocking monoclonal antibodies. The method comprises the following steps: sorting plasma cells and memory single B cells for specifically recognizing TSHR in peripheral blood of a patient with high TSH-blocking antibody (TBAb) titer by using flow cytometry, cloning antibody light and heavy chains in vitro and performing recombinant expression, and performing antibody property screening verification by using hTSHR-CHO cells, so as to obtain the TSHR-TBAb-TSHR-TBAb. The blocking monoclonal antibody specifically targeting the human TSHR is obtained. The fully human TSH receptor blocking monoclonal antibody disclosed by the invention can be specifically combined with TSHR, and can effectively block signal transduction after the TSH is combined with the receptor; the synthesis and secretion of thyroid hormone are inhibited; according to the present invention, with the application of the compound, the expression and the fibrosis of the effector cell orbit fibroblast TSHR inhibiting the thyroid-related eye disease can be significantly reduced, and the wide application prospect is provided in the treatment of Graves' disease (Graves' disease, GD) and diseases caused by hyperthyroidism, such as thyroid eye disease (thyroid eye disease, TED), and the like.

The invention belongs to the technical field of biological medicine, and particularly relates to a fluorescent probe targeting SHP2, a preparation method and application of the fluorescent probe. A core pharmacophore is determined by replacing an amino group in a central region B of SHP2-099 into a p-methyl group and introducing a sulfur atom as a connecting atom, an SBD fluorophore is integrated in the core pharmacophore, and a series of SHP-PS (1-4) targeting to SHP2 and having connecting arms with different lengths is developed. The series of SHP2-targeting fluorescent probes provided by the invention have high specificity and good target affinity, can selectively mark SHP2 protein in living cells, have an environmentally sensitive switch mechanism, provide a new tool for researching the effect of SHP2 in signal transduction and the significance of SHP2 in disease progression, and have broad application prospects. The research on diseases such as cancer is expected to be accelerated, and a new way is provided for diagnosis and treatment.

The invention relates to an anti-VEGF antibody, a heavy chain variable region sequence is shown as SEQ ID NO: 1, and a light chain variable region sequence is shown as SEQ ID NO: 2. According to the present invention, the monoclonal antibody capable of specifically binding to VEGF is prepared, the binding of VEGF and its receptor can be inhibited after the monoclonal antibody is bound to VEGF, the downstream signal transduction pathway of VEGF is blocked, and the animal test results show that the antibody can inhibit the growth of glioblastoma.

The invention discloses long-acting high-dynamic hydrazone bond hydrogel as well as a preparation method and application thereof, and particularly relates to the technical field of biomedical materials. The preparation method comprises the following steps: taking 1-adamantane acetic acid and adipic dihydrazide modified sodium hyaluronate and benzaldehyde-terminated eight-arm polyethylene glycol as carrier raw materials; the hydrazone bond hydrogel with relatively high stability is prepared through a coupling reaction of an aldehyde group of benzaldehyde-terminated eight-arm polyethylene glycol and a hydrazide group of 1-adamantane acetic acid and adipic dihydrazide modified sodium hyaluronate. The beta-cyclodextrin modified m-phenylenediamine catalyst is dynamically combined into the carrier through reversible host-guest complexation between beta-cyclodextrin and 1-adamantane acetic acid, so that the long-acting high-dynamic hydrazone bond hydrogel is obtained, and the requirement of three-dimensional culture of stem cells on mechanical properties can be met; and network dynamics can be maintained for a long time, so that stem cell spreading is supported, mechanical signal transduction is activated, osteogenesis-related gene expression is promoted, and stem cell osteogenesis differentiation capability is enhanced.

The invention discloses an MT (melatonin) and SNP (single nucleotide polymorphism) compound preservative as well as preparation and application thereof, and relates to the field of compound preservative films. A nitric oxide donor sodium nitroprusside (SNP); tween-80 (Tween-80) is added into auxiliary components to serve as an emulsifying agent, MT and SNP are dissolved in a proper amount of water and evenly stirred, then Tween-80 is added, and the MT and SNP compound preservative is prepared, according to the MT and SNP compound preservative and preparation and application thereof, MT and SNP are natural or quasi-natural components, compared with chemical preservatives, the MT and SNP have smaller potential harm to the environment and the human body, MT and SNP are used in a compound mode, and therefore the MT and SNP compound preservative has the beneficial effects that the MT and the SNP are more environmentally friendly and more environmentally friendly. MT and SNP cooperate with each other to improve the fresh-keeping effect, the antioxidant effect of MT can reduce oxidative damage in the fruits, SNP promotes endogenous NO generation, can regulate signal transduction in the fruits and further inhibits respiration and ethylene synthesis of the fruits, maturing and aging of the fruits are delayed from different angles through cooperation of MT and SNP, and the fresh-keeping effect of the fruits is improved. Meanwhile, the MT and the NO can cooperatively regulate and control signal transduction in the plant body, and the action time of the NO in the fruit body is prolonged.

The invention discloses a rice bakanae disease prevention and treatment medicine and a preparation method thereof, and belongs to the technical field of agricultural disease prevention and treatment. The medicine for preventing and treating the rice bakanae disease is prepared from the following raw materials in parts by mass: 4 to 8 parts of cyrtomium rhizome extract, 0.5 to 1.5 parts of pydiflumetofen hydroxylamine, 0.1 to 0.5 part of isoxazole formic acid, 0.01 to 0.1 part of compound sodium nitrophenolate, 1 to 2 parts of surfactant, 0.1 to 2 parts of dispersant, 0.1 to 2 parts of stabilizer, 0.1 to 1 part of antioxidant and 90 to 100 parts of carrier. Through the synergistic effect of the cyrtomium fortunei extract, the pydiflumetofen and the isoxadifen carboxylic acid with different action mechanisms, the rice bakanae disease morbidity is remarkably reduced in a mode of simultaneously inhibiting growth and reproduction of pathogenic bacteria and transduction of a plant spindling signal, and the generation of resistance of the pathogenic bacteria is delayed.

Provided is a bispecific chimeric antigen receptor targeting CD38 and CLL1. The chimeric antigen receptor at least comprises, from the N-terminus to the C-terminus: i) an antigen-binding domain that specifically recognizes CLL1 and CD38; ii) a transmembrane domain; iii) at least one co-stimulatory domain; and iv) a signaling domain. A dual-targeting CAR-T cell prepared by using the chimeric antigen receptor described above shows highly specific in-vitro cytotoxicity against CLL1-positive and / or CD38-positive target cells.

The invention belongs to the technical field of biology, and particularly relates to application of baicalin to preparation of a medicine for treating preeclampsia by inhibiting trophoblast ferroptosis induced by NETs. The baicalin inhibits downstream signal transduction by specifically interfering a key activation step of PKC [beta] 2 and a membrane translocation process coupled with a second signal, and finally plays a role in inhibiting ferroptosis of trophoblasts. The discovery provides a brand new perspective for understanding the molecular action mechanism of the medicine. According to the application, baicalin influences ferroptosis induced by NETs in trophoblast cells by inhibiting membrane translocation and activation of PKC beta2, so that the biological function of baicalin is exerted. The discovery of the mechanism further deepens the important significance of people on NETs and baicalin in disease induction and treatment, and a thought is provided for research and development of a new treatment scheme for preeclampsia.