534 results about "Signal transduction" patented technology

Disclosed is a system for detecting whether the breast cancer is benign or malignant based on multiple modalities, which comprises: an ultrasonomics model configured to extract breast tumor position information and breast tumor morphological features on the basis of an acquired grayscale breast ultrasound image, perform global interactive fusion on the breast tumor position information and breast tumor morphological feature information, and convert a Transformer encoder-based feature output into an output vector of the corresponding classification dimensions by means of a fully connected layer; a metabonomics model configured to obtain a metabolic fingerprint spectrogram and analyze the metabolic fingerprint spectrogram on the basis of a metabolic database and a predetermined significantly differential metabolite to obtain abnormal metabolic pathways, signal transduction pathways, and related biochemical reactions associated with breast cancer malignancy; and a detection module configured to perform joint modeling, based on the breast tumor position information, the breast tumor morphological features, and a peak value of the significantly differential metabolite of the breast cancer malignancy, to output a prediction result about whether the breast cancer is benign or malignant.

The invention relates to bionic matrix hydrogel for constructing bone organs and a preparation method of the bionic matrix hydrogel. The bionic matrix hydrogel is prepared from a bone acellular extracellular matrix, a salmon DNA (Deoxyribose Nucleic Acid) network and a calcium phosphate oligomer under the combination of photo-crosslinking and self-assembly physical crosslinking. The acellular extracellular matrix in the bionic matrix hydrogel can simulate organic components in natural bones, the calcium phosphate oligomer can simulate inorganic components in the natural bones, and sufficient phosphate ions can be provided for biomineralization due to introduction of a DNA network. The biomimetic matrix hydrogel can replace Matrigel to act in bone organoid culture, provide a living environment similar to bone tissue for three-dimensional culture cells, enhance substance exchange and signal transduction between cells, promote proliferation, adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells, and further accelerate the process of biomineralization. The invention provides an optional new material for preparing the functional bone organ.

The invention relates to the technical field of agricultural irrigation, in particular to an intelligent biological irrigation system and method based on remote control, and the system comprises a biological sensing unit, a neural computing decision engine, a driving execution unit, and a communication optimization architecture. Wherein the biological sensing unit is used for monitoring multi-modal data of plant physiological status, rhizosphere microenvironment and meteorological parameters in real time; the neural computing decision engine is used for constructing a pulse neural network, fusing a plant dimensional management system signal transduction mechanism and a multi-agent optimization algorithm, and generating a millisecond-level dynamic irrigation strategy; the driving execution unit is used for millimeter-level water quantity adjustment and pipe network self-repairing of the irrigation execution unit; the communication optimization architecture is used for seamless coverage and secure transmission of irrigation data, and meanwhile, a strategy is optimized by applying algorithms such as reinforcement learning and the like. Therefore, the problems of water resource waste, poor irrigation effect, untimely irrigation strategy adjustment and the like in the prior art are solved.

The invention provides a nano photosensitizer modified by outer membrane vesicles of genetically engineered bacteria as well as a preparation method and application of the nano photosensitizer. The nano photosensitizer is a nano material with the particle size of 80-120 nm, and the nano photosensitizer modified by the genetically engineered bacterium outer membrane vesicle comprises dipalmitic acid phosphatidylcholine (DPPC), distearoyl phosphatidylcholine (DSPC), dioleoyl lecithin (DOPC), cholesterol, a small molecule dye and CD47nb-OMV membrane protein. The chemical structure of the small molecule dye is shown as a formula I or a formula II. The nano photosensitizer OBDlp modified by outer membrane vesicles of genetically engineered bacteria serves as a novel nano photosensitizer, tumor targeting PTT is mediated through NIR II FL / PA imaging, tumor immunogenicity is improved, antigen presenting cells are activated, and infiltration and functionalization of CD8 + T cells are caused. And OBDlp and laser are combined to induce release of a large amount of DAMPs and trigger ICD to promote maturation of cDC1s. Meanwhile, a CD47 nano antibody carried by the nano photosensitizer induces polarization of M1 phenotype macrophages by blocking a CD47-SIRP alpha signal transduction axis, and an immunosuppression tumor microenvironment is reversed.

The invention provides a preparation method of a recombinant collagen dressing, and belongs to the technical field of medical dressings. According to the technical scheme, stacked short sequences and continuous human collagen amino acid short sequences are repeatedly combined for multiple times to form recombinant collagen with specificity, and high similarity of the recombinant collagen with human collagen is ensured. On the basis, 4-FPBA is used as a cross-linking agent to respectively form a Schiff base bond responsive to pH and a boric acid ester bond responsive to glucose with CMCS and two kinds of HA with different molecular weights, and then the double-dynamic covalent cross-linked network adhesive hydrogel is synthesized. The triple-helix structure of the recombinant collagen is combined with a specific receptor on a cell membrane, and cell behaviors are regulated and controlled by inducing signal transduction through a cytoskeleton, so that skin microcirculation can be effectively improved, and a skin barrier can be repaired; the hydrogel dressing matrix has the effects of resisting inflammation, resisting oxidation, resisting bacteria, promoting healing and the like, the biological performance of the dressing is remarkably improved, and a better curative effect is achieved.

The invention belongs to the technical field of protein, and relates to a peptide for promoting synthesis of a brain-derived neurotrophic factor (BDNF) as well as a preparation method and application of the peptide. The amino acid sequence of the peptide for promoting synthesis of the brain-derived neurotrophic factor BDNF is SGFSKHF, and the peptide has the main effects that damage of CORT (corticosterone) to PC12 cells can be protected, the peptide can be spontaneously combined with a TrkB receptor, the structure of a compound formed after combination is more stable, BDNF-TrkB-CREB pathway signal transduction can be activated, generation of the BDNF is promoted, and the effect of promoting synthesis of the brain-derived neurotrophic factor BDNF is achieved. Therefore, the occurrence and development of neurodegenerative diseases can be delayed.

The invention discloses an anti-neurodegeneration exosome composition as well as a preparation method and application thereof. The anti-neurodegeneration exosome composition is prepared from the following components in parts by mass: 5 to 25 parts of human exfoliated deciduous tooth stem cell exosome, 25 to 85 parts of plant-derived exosome-like nano-vesicles, 12 to 10 parts of ginsenoside Rg, 2 to 10 parts of curcumin and 10 to 20 parts of auxiliary materials. The anti-neurodegeneration exosome composition contains a large amount of cell factors, growth factors, miRNA, mRNAs and other components, is easy to fuse with cell membranes of intestinal epithelial cells, can selectively deliver bioactive substances to recipient cells through a blood-brain barrier, carries out information transmission among different cells, regulates signal transduction among the cells, and has a good anti-neurodegeneration effect. The effects of resisting oxidation, resisting inflammation, resisting aging and the like are achieved.

The invention discloses an E-CAR-NK cell for treating systemic lupus erythematosus and application of the E-CAR-NK cell, and belongs to the technical field of genetic engineering.A CD19 and BCMA double-target E-CAR is adopted for modifying an NK cell, and the E-CAR-NK cell is obtained; the CD19 and BCMA double-target E-CAR is composed of the following modules: a CD8 signal peptide, a CD19 scFv, a Linker, a BCMA scFv, a CD8 Hinge region, an NKG2D transmembrane region, a CD3 epsilon signal transduction region, a DAP12 costimulatory region, a 2B4 costimulatory region, and a CD3 zeta signal transduction region. Compared with a common CAR-NK cell, the E-CAR-NK cell disclosed by the invention has higher antigen sensitivity and better continuous killing ability, so that the treatment effect of SLE is improved, and the E-CAR-NK cell has lower side effects and higher safety.

The invention discloses a tea tree CsBZR1 gene and cold resistance application thereof, the tea tree CsBZR1 gene is a core gene in a tea tree brassinolide signal transduction pathway, and the nucleotide sequence of the tea tree CsBZR1 gene is shown as SEQ ID NO.1 in a sequence table. According to the application of the CsBZR1 gene of the tea tree to regulation and control of cold resistance of the tea tree, the low-temperature tolerance of the tea tree can be reduced by inhibiting expression of the CsBZR1 gene in vivo, and the cold resistance of arabidopsis thaliana is improved by overexpression of the CsBZR1 gene. Expression of CsBZR1 of a tea tree under a low-temperature condition is directly induced to be up-regulated by low-temperature stress of the tea tree, cold resistance of arabidopsis thaliana is improved by overexpression of the gene in the arabidopsis thaliana, and low-temperature damage of the tea tree can be remarkably aggravated by inhibiting expression of CsBZR1 in the tea tree through an antisense oligonucleotide technology. The cloning of the gene not only is beneficial to determining the effects of brassinolide and the signal transduction core factor BZR1 thereof in the cold-resistant process of the tea tree, but also is beneficial to promoting the genetic improvement process aiming at enhancing the cold resistance of the tea tree.

The multi-channel electrochemical micro-fluidic chip detection platform provided by the invention realizes rapid, high-throughput, low-cost, high-sensitivity and specific detection on genital tract pathogens. Specifically, when a target exists, a target recognition probe Cas12a-crRNA compound specifically recognizes a target and forms a Cas12a-crRNA-Target triad, the trans-cleavage activity is activated, an activated Cas12a system cuts a G1 / G2 single-chain region in the signal amplification probe, then encapsulated methylene blue is released, the methylene blue further promotes a methyl bromide redox reaction, signal transduction is promoted, and the target recognition probe Cas12a-crRNA compound can be used for recognizing the target. Therefore, high-sensitivity and high-specificity quantitative detection of pathogens is realized. On the basis, through a multi-channel micro-fluidic chip, high-sensitivity detection of multiple targets can be realized at the same time by placing different crRNA sequences in reaction tanks respectively. In the embodiment of the invention, the multi-target ultra-sensitive detection of CT, CA, TV and NG is successfully realized, and the detection limit is as low as 0.8 fM.

The present invention relates to a cell comprising a chimeric antigen receptor (CAR) and a constitutively active or inducible Signal Transducer and Activator of Transcription (STAT) molecule.

Provided are a recombinant human interleukin-11 mutant, and a conjugate of the mutant conjugated to a chemical molecule and a use thereof. The recombinant human interleukin-11 mutant achieves soluble expression in an Escherichia coli expression system by means of an SUMO sequence tandem fusion method. The conjugate is obtained by the site-specific conjugation of the mutant to the chemical molecule at a mutant amino acid residue in position W147 by means of a covalent bond. The chemical molecule is at least one of polyethylene glycol, an alkanoic acid aliphatic chain, and a hydrophilic high-molecular polymer containing an alkanoic acid aliphatic chain. The conjugate retains the binding capacity to human interleukin-11 receptor A while demonstrating significantly reduced binding capacity to human IL-6 signal transducer receptor. The conjugate shows the ability to block the signaling between human IL-11 and IL-11 RA and between human IL-11 and GP130 either in vivo or in vitro and can be used for the treatment of associated diseases with tissue fibrosis as a clinical manifestation caused by elevated primary or secondary IL-11.

The invention discloses a preparation method and application of a novel hybrid extracellular vesicle. According to the invention, vesicles derived from endothelial cells and neutrophil cells are combined with deferoxamine, and a biological mixed nano vesicle platform (DFO (HEVS)) is developed to solve the problem of diabetes wound healing. According to the dual-targeting system, DFO is accurately delivered to a wound part by utilizing CXCR4 mediated endothelial cell homing and beta2 integrin dependent inflammation tropism, DFO (at) HEVs activates and recovers vascular regeneration through HIF-1alpha / VEGF, ferroptosis is inhibited through Nrf2 / GPX4 signal transduction, macrophages are reprogrammed into a repair promoting M2 phenotype, and oxidative stress-inflammation-ferroptosis circulation is effectively broken.

The provided long-acting kinin or the derivative thereof fundamentally solves the problem that kinin has no druggability, on one hand, the half-life period of kinin or the derivative thereof is prolonged through a long-acting technology, and it is prevented that kinin or the derivative thereof is rapidly degraded and inactivated in vivo; on the other hand, the action site of the kinin and the receptor is covered or partially covered through long-acting modification, so that the adverse reactions such as rapid blood pressure reduction and hypotension in a short time are effectively avoided. The long-acting kinin or the derivative thereof well retains receptor binding activity and signal transduction activity in an in-vitro cell experiment, can effectively treat hypertension and prevent or treat hypertension complications in an in-vivo experiment, and provides a new method for treating hypertension and hypertension complications.

The invention discloses a light-activated chemiluminescence immunodetection method for rapidly quantifying C-reactive protein (CRP), and belongs to the technical field of marker detection. Comprising a luminous microsphere-CRP coated antibody complex, a biotin-CRP labeled antibody complex and a streptavidin coated photosensitive microsphere. The light-sensitive microspheres and the light-emitting microspheres are ingeniously combined, and key factors such as coupling efficiency of antibodies on the surfaces of the light-emitting microspheres, reagent dosage, incubation temperature and time and the like are elaborately optimized, so that an optimized light-activated chemiluminescence detection system is constructed. The system not only simplifies the experimental process, but also ensures the reliability and consistency of the detection result by enhancing the stability and specificity of signal transduction. By screening and optimizing conditions of the system, the invention successfully develops a novel CRP (C-reactive protein) light-activated chemiluminescence immunoassay kit, and the detection limit is 6.20 ng.mL <-1 >.

The invention relates to the technical field of biological medicine, in particular to a lymphatic metastatic breast cancer treatment target and application thereof. The exosome piR-hsa-28212 disclosed by the invention can synergistically enhance VEGFC / VEGFR3 signal transduction, so that lymphatic vessel generation in breast cancer is promoted, and the potential of the exosome piR-hsa-28212 serving as a treatment target of lymphatic metastatic breast cancer is indicated. Besides, piR-hsa-28212 in the serum exosome of the breast cancer patient with lymphatic metastasis is obviously up-regulated, so that the piR-hsa-28212 can be used as a serum marker for predicting the lymphatic metastasis of the breast cancer, and has the advantages of noninvasive property, in-vitro stability, acquisition of samples and the like.

The invention provides a naked eye visual detection method based on CRISPR / Cas reaction system nucleic acid detection and a kit thereof, in the method, a solution of a CRISPR / Cas reaction system is prepared, the solution contains a Cas enzyme with trans-cleavage ability, crRNA and an SSA capture probe, the SSA capture probe is constructed as a recognition element by combining a CRISPR / Cas signal amplification system, and the SSA capture probe is used for detecting nucleic acid in the CRISPR / Cas reaction system. An artificially programmed CG probe with a color developing function, which specifically responds to an SSA probe, is used as a signal transduction element, the SSA probe is used for effectively capturing the CG probe, target nucleic acid is used for activating the activity of CRISPR / Cas trans-cleavage, and the SSA and the CG probe are cleaved, so that colloidal gold is in a free state in a solution, and after magnetic separation is observed by naked eyes, the CG probe and the SSA probe are separated by magnetic force. When the detected sample is positive, the supernatant color is pink, the supernatant of the negative sample and the supernatant of the blank sample are in a colorless state, and the target nucleic acid is detected by distinguishing the colors.

Methods for interfering or controlling IFN induction, IFN production and / or IFN activity are disclosed, harnessing viral mechanisms for evading or suppressing host anti-viral immune reactions. These methods comprise administration of one or more viral messenger RNAs (mRNAs) coding for proteins or peptides that control or interfere with signal transduction upstream and / or downstream of IFN induction, thereby providing a means for preventing or treating diseases, disorders or conditions associated with increased IFN production, such as cancer, autoimmune diseases, inflammatory diseases and interferonopathies.

The invention discloses a method for screening a Wilson disease serum exosome miRNA biomarker based on a transcriptomics technology, and belongs to the field of bioinformatics analysis of diseases. According to the invention, the effect of miRNA in WD pathogenesis is discussed by identifying serum exosome miRNA, and a potential biomarker is provided for accurate diagnosis and treatment of the disease. According to the invention, 59 DE-miRNAs are identified by analyzing the expression of differential miRNAs of WD and control group patients, and the DE-miRNAs are related to key approaches such as metabolic regulation, cancer progression, signal transduction and the like. Besides, the reliability of the key DE-miRNA, including miR-451a, miR-204-5p and the like, is confirmed through experimental verification, and the potential of the key DE-miRNA as a non-invasive biomarker is indicated. Research results provide important theoretical basis and new insight for accurate diagnosis of WD and development of potential therapeutic targets.

The invention provides an anti-CD40 monoclonal antibody and application of the anti-CD40 monoclonal antibody. The anti-CD40 monoclonal antibody comprises three heavy chain complementarity determining regions (HCDR1, HCDR2 and HCDR3) as shown in SEQ ID NOs: 1-3, and three light chain complementarity determining regions (LCDR1, LCDR2 and LCDR3) as shown in SEQ ID NO: 4, YAS and SEQ ID NO: 5. The antibody has binding activity with in-vitro CD40 and CD40 on the cell surface, and can be competitively bound with CD40L to block binding of the antibody and CD40L so as to inhibit CD40 / CD40L signal transduction and inhibit B cell proliferation and activation, so that immune overexpression and rejection can be inhibited. Therefore, when the antibody is applied to preparation of drugs for treating immune diseases, the antibody has the advantages of high targeting specificity, high affinity, few side effects and the like, and has a great application prospect.

The invention relates to an IL5RA cell surface marker. A cell tag comprising an extracellular region, a transmembrane region, and optionally an intracellular region is provided. The extracellular region comprises an IL5 receptor alpha (IL5Ra) sequence linked to a transmembrane domain, and the recombinant polypeptide cannot function in signal transduction. The cell tag can be operably linked to a transgene. Expression of the cell tag allows for the identification, detection, selection, and ablation of cells expressing the transgene and the cell tag. In some embodiments, the genetically modified host cell comprises a transgene comprising a polynucleotide encoding a chimeric antigen receptor comprising a ligand binding domain and a polynucleotide encoding a cell tag. Pharmaceutical formulations produced by the methods and methods of using the pharmaceutical formulations are also described.

The invention provides a method for constructing an MDA-MB-231 cell strain for expressing moesin-EGFP (Enhanced Green Fluorescent Protein). According to the method, sgRNA of a targeted moesin gene terminator region is designed, a Donor plasmid containing LHA, Linker, EGFP and RHA sequences is constructed, and a connecting sequence is added between a moesin protein and an EGFP protein by the plasmid, so that the influence of the EGFP on the function of the moesin protein is avoided. The sgRNA, the Cas9 protein and the linearized Donor plasmid are jointly transferred into the MDA-MB-231 cell by utilizing a CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) gene editing technology. According to the method, homozygote cells for expressing moesin-EGFP (enhanced green fluorescent protein) are sorted through flow cytometry, and gene editing and protein expression conditions are verified through PCR (polymerase chain reaction) and Western blot. According to the method, stable expression of the moesin-EGFP fusion protein is achieved, and the purpose of monitoring endogenous moesin expression in cells in real time is achieved. The cell strain can be used for researching the function of moesin in cell migration and signal transduction, and has a wide application prospect.

The invention discloses an antibody combined with interleukin 4 receptor (IL-4R) protein and a computer optimization method, a compound structure analysis method and application thereof, the antibody comprises a Fa-2 sequence, a Fa-3 sequence, a Fa-4 sequence, a Fa-5 sequence, a Fa-7 sequence, a Fa-13 sequence, a Fa-15 sequence, a Fa-16 sequence, a Fa-17 sequence, a Fa-19 sequence and a Fa-23 sequence, and the Fa-2 sequence, the Fa-3 sequence, the Fa-4 sequence, the Fa-5 sequence, the Fa-7 sequence, the Fa-13 sequence, the Fa-15 sequence, the Fa-16 sequence, the Fa-17 sequence, the Fa-19 sequence and the Fa-23 sequence comprise a heavy chain variable region and a light chain variable region. The amino acid sequences of the amino acid sequences are respectively shown as SEQ ID NO: 7, 8, 9, 10, 11, 12, 13, 14, 17, 18, 29, 30, 33, 34, 35, 36, 37, 38, 41, 42, 49 and 50. According to the invention, a brand-new IL-4R epitope is found, and an antibody with a'non-ligand blocking 'characteristic is obtained for the first time. The invention opens up a brand new path for developing a therapy for intervening the IL-4R pathway through a new mechanism of regulating signal transduction or interacting with other coreceptors and the like, and is expected to overcome the limitation of the existing therapy. According to the method, computational biology and experimental structure biology are seamlessly connected, a set of repeatable and efficient research and development process is formed, and the method has a wide application prospect.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.

The invention relates to application of a paddy BR signal transduction haplotype (BSHs) in indica-japonica subspecies differentiation, yield increase and quality improvement. Specifically, the invention relates to the application of two main BR signal transduction haplotypes formed by co-separation or co-selection of main variations existing in four identified genetically non-linked key components (OsBRI1, OsBAK1, OsGSK3 and OsBZR1) of a rice brassinosteroid signal transduction pathway, and the application of the two main BR signal transduction haplotypes to differentiation, yield increase and quality improvement of indica-japonica subspecies of rice. The genetic contribution of the BSHs to the rice yield and quality is far higher than the contribution of the four components independently, the two main BSHs (BSH2-J and BSH6-I) can be used for genetic improvement of the rice yield and quality traits, and compared with the BSH2-J, the BSH6-I has weaker BR signal strength and BR sensitivity, so that the yield and quality traits of the BSH6-I are superior to those of the BSH2-J.

The invention discloses a group of blocking monoclonal antibodies of an SH1 homologous cluster fully human thyrotropin receptor (TSHR) (a TSH receptor, TSHR) as well as a preparation method and application of the blocking monoclonal antibodies of the SH1 homologous cluster fully human thyrotropin receptor (TSHR). The method comprises the following steps: sorting plasma cells and memory single B cells for specifically recognizing TSHR in peripheral blood of a patient with high TSH receptor blocking antibody (TBAb) titer by using flow cytometry, cloning antibody light and heavy chains in vitro and performing recombinant expression, and performing antibody property screening verification by using hTSHR-CHO cells. The blocking monoclonal antibody specifically targeting the human TSHR is obtained. The fully human TSH receptor blocking monoclonal antibody disclosed by the invention can be specifically combined with TSHR, and can effectively block signal transduction after the TSH is combined with the receptor; the synthesis and secretion of thyroid hormone are inhibited; expression and fibrosis of effector cell orbital fibroblasts TSHR for inhibiting thyroid-related eye diseases are remarkably relieved, and wide application prospects are achieved in treatment of Graves' diseases and diseases such as thyroid eye diseases caused by hyperthyroidism.

The invention discloses a chimeric antigen receptor targeting an HIV infected cell, a CAR-T cell and application. The chimeric antigen receptor comprises an antigen binding domain, a chimeric receptor hinge region, a transmembrane domain, a costimulatory signal transduction domain and a signal transduction domain, the antigen binding structural domain is an anti-HIV monoclonal antibody JSR771 and is composed of a heavy chain VH and a light chain VL, the amino acid sequence of the heavy chain VH is as shown in SEQ ID NO.1, and the amino acid sequence of the light chain VL is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the anti-HIV monoclonal antibody JSR771. By optimizing the separation, activation, transduction and culture conditions of the T cells and strictly controlling the quality control standard, the prepared anti-HIV-1CAR-T cells have high motility, high specificity and strong killing ability, and a new hope is provided for clinical treatment of HIV infection.

The invention discloses a hydrogen peroxide sensing electrode, a preparation method thereof, a hydrogen peroxide electrochemical sensor and a monitoring method. The hydrogen peroxide sensing electrode comprises a tube body, the tube body is provided with a first end and a second end, the first end is provided with a first opening, the second end is a tip, the tip is provided with a second opening, the first opening is communicated with the second opening, and the caliber of the second opening is smaller than that of the first opening; the tip is filled with solid hydrogel embedded with catalase, and the aperture of the solid hydrogel is 20-50 nm; the tube body is filled with an electrolyte, and the electrolyte is a solution with ionic conductivity; the electrolyte is in contact with the solid hydrogel. According to the invention, nano confinement hydrogel and electrophoresis-like technologies are combined, oxygen nano bubbles are used as a signal transduction mechanism, ultrasensitive detection of intracellular H2O2 is realized through ionization sensing, and dynamic change of intracellular hydrogen peroxide can be monitored in situ in real time.

The invention provides a pathogen viable bacteria detection kit based on endogenous protein activated deoxyribozyme. The kit comprises a deoxyribozyme recognition element and a signal transduction element. The deoxyribozyme recognition element is composed of a substrate chain and an enzyme chain, and the signal transduction element is composed of two DNA reporter probes (Sub-F and Sub-Q), wherein the tail ends of the two DNA reporter probes are respectively marked with a fluorescence staining group and a fluorescence quenching group. The deoxyribozyme recognition element can recognize specific endogenous protein of target pathogenic viable bacteria and activate an enzyme chain to cut a substrate chain to generate two independent substrate chain fragments. And then a signal transduction element is introduced to realize specific output of a fluorescence signal. The kit provided by the invention can realize detection of living salmonella by targeting endogenous protein, can sensitively detect 190 CFU / mL of living salmonella with abundance as low as 0.1%, and has a good application prospect in the directions of food safety detection and clinical examination.