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Urine microalbumin detection kit and preparation method thereof

A urine microalbumin and detection kit technology, which is applied in the field of medical immunology in vitro diagnosis, can solve the problem of low sensitivity, achieve high sensitivity, simple detection, and improve the effect of detection sensitivity

Inactive Publication Date: 2017-06-20
吉林省富生医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the detection principle of immunoturbidimetry is to react to generate insoluble antigen-antibody complexes and generate a certain turbidity, the level of turbidity reflects the content of microalbumin in the sample, resulting in the existing urinary microalbumin kits Inevitable lack of low sensitivity

Method used

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  • Urine microalbumin detection kit and preparation method thereof
  • Urine microalbumin detection kit and preparation method thereof
  • Urine microalbumin detection kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A urine microalbumin detection kit, comprising reagent R1 and reagent R2 independent of each other, its composition is as follows:

[0047] Reagent R1, its components are:

[0048]

[0049]

[0050] Reagent R2, its component is:

[0051]

Embodiment 2

[0053] A urine microalbumin detection kit, including reagent R1 and reagent R2 independent of each other, its composition is as follows: reagent R1, its components are:

[0054]

[0055] Reagent R2, its component is:

[0056]

[0057]

Embodiment 3

[0059] Preparation and use of the kit:

[0060] 1. Reagent preparation

[0061] A. Preparation of reagent R1:

[0062] Add NaCl 0.5%, PEG6000 25g / L, bovine serum albumin 10g / L, and sodium azide 1g / L to MES buffer 50mmol / L in sequence, stir and mix evenly, and filter with a 0.22 micron mixed cellulose membrane to obtain reagent R1 ;

[0063] B. Preparation of reagent R2:

[0064] Add NaCl 0.5%, sodium azide 1g / L, bovine serum albumin 10g / L, glycerin 30mmol / L, anti-human albumin antibody 3.0g / L to PBS buffer 50mmol / L and stir to mix evenly. The mixed cellulose membrane was filtered to obtain the reagent R2.

[0065] 2. Parameter setting of automatic biochemical analyzer

[0066] a. Detection temperature: 37°C;

[0067] b. Detection wavelength: main wavelength 340nm, secondary wavelength 700nm;

[0068] c. Reaction time: 10 minutes, among them, the incubation time is 5 minutes, read the absorbance A1 immediately after adding the reagent R2, read the absorbance A2 after 5 m...

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Abstract

The invention relates to a urine microalbumin detection kit and a preparation method thereof. The detection kit is prepared from a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from buffer solution (1), inorganic salt ions (1), accelerator (1), stabilizer (1) and preservative (1), and the reagent R2 is prepared from buffer solution (2), inorganic salt ions (2), preservative (2), stabilizer (2), suspending aid (1) and anti-human albumin antibody. The preparation method of the detection kit disclosed by the invention comprises the steps of preparing reagents according to constituent content; mixing a sample to be tested with the reagent R1 and the reagent R2 to enable the sample, the reagent R1 and the reagent R2 fully react; utilizing a full-automatic biochemical analyzer to measure an absorbance difference value after reaction; calculating out the concentration of urine microalbumin in the sample according to an absorbance change value. The detection kit disclosed by the invention has the advantages of low equipment cost, high sensibility, good accuracy and precision, easiness to store, good data repeatability and wide application to clinical biochemical analyzers.

Description

technical field [0001] The invention belongs to the field of in vitro diagnosis of medical immunity, and in particular relates to a urine microalbumin detection kit and a preparation method thereof. Background technique [0002] The albumin in the urine of normal people is usually below 20mg / L. When the urine albumin concentration rises at a low level, ranging from 20-200mg / L, it is called microalbuminuria, and the protein in the urine at this time is called urine. Microalbumin. When urinary microalbuminuria is greater than 300mg / 24h, it is called macroalbuminuria. Increased urinary microalbumin excretion is an early change in the disease, which has important reference value and clinical significance for early diagnosis and early treatment of the disease. [0003] Urinary microalbumin detection is an important diagnostic tool for screening high-risk groups of early kidney disease. Patients with diabetes (type 1, type 2) and hypertension should be the main population to be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6827
Inventor 张海悦程慧敏孙景春闫小娟魏滢王姝韩永双韩峰刘玉娟
Owner 吉林省富生医疗器械有限公司
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